Citation:

PANGAEA.937391

Name: Seawater carbonate chemistry and bioinformatic quality statistics
Published: 2021-10-15
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Crassostrea gigas; Gene expression (incl. proteomics); Laboratory experiment; Mollusca; North Pacific; Single species; Temperate

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Abstract:

The adaptive capacity of marine calcifiers to ocean acidification (OA) is a topic of great interest to evolutionary biologists and ecologists. Previous studies have provided evidence to suggest that larval resilience to high pCO2 seawater for these species is a trait with a genetic basis and variability in natural populations. To date, however, it remains unclear how the selective effects of OA occur within the context of complex genetic interactions underpinning larval development in many of the most vulnerable taxa. Here we evaluated phenotypic and genetic changes during larval development of Pacific oysters (Crassostrea gigas) reared in ambient (400 µatm) and high (1600 µatm) pCO2 conditions, both in domesticated and naturalized 'wild' oysters from the Pacific Northwest, USA. Using pooled DNA samples, we determined changes in allele frequencies across larval development, from early “D-stage” larvae to metamorphosed juveniles (spat), in both groups and environments. Domesticated larvae had 26% fewer loci with changing allele frequencies across developmental stages and < 50% as many loci affected by acidified culture conditions, compared to larvae from wild brood stock. Functional enrichment analyses of genetic markers with significant changes in allele frequency revealed that the structure and function of cellular membranes were disproportionately affected by high pCO2 conditions in both groups. These results indicate the potential for a rapid adaptive response of oyster populations to OA conditions; however, underlying genetic changes associated with larval development differ between these wild and domesticated oyster stocks and influence their adaptive responses to OA conditions.

Keyword(s):

Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Crassostrea gigas; Gene expression (incl. proteomics); Laboratory experiment; Mollusca; North Pacific; Single species; Temperate

Related to:

Durland, Evan; de Wit, Pierre; Meyer, Eli; Langdon, Chris (2021): Larval development in the Pacific oyster and the impacts of ocean acidification: Differential genetic effects in wild and domesticated stocks. Evolutionary Applications, 14(9), 2258-2272, https://doi.org/10.1111/eva.13289

Source:

Durland, Evan; de Wit, Pierre; Meyer, Eli; Langdon, Chris (2021): Full output of functional enrichment analyses and gene annotations for membrane functions. Durlan_etal_2021_Raw_Data.rar

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-10-14.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Durland, Evan		study
2	Species	Species		Durland, Evan
3	Registration number of species	Reg spec no		Durland, Evan
4	Uniform resource locator/link to reference	URL ref		Durland, Evan		WoRMS Aphia ID
5	Group	Group		Durland, Evan
6	Treatment	Treat		Durland, Evan
7	Age	Age	days	Durland, Evan
8	Replicate	Repl		Durland, Evan
9	Reads	Reads	#	Durland, Evan		Raw
10	Reads	Reads	#	Durland, Evan		Quality
11	Reads	Reads	#	Durland, Evan		matched to illumina adaptor seq
12	Reads	Reads	#	Durland, Evan		Quality and clean
13	Reads	Reads	#	Durland, Evan		Mapped
14	Percentage	Perc	%	Durland, Evan		mapped
15	Ratio	Ratio		Durland, Evan		Avg hits/ matched read
16	Reads	Reads	#	Durland, Evan		Raw mappings
17	Reads	Reads	#	Durland, Evan		>=1 match
18	Reads	Reads	#	Durland, Evan		Weak match
19	Reads	Reads	#	Durland, Evan		Ambiguous match
20	Reads	Reads	#	Durland, Evan		Unique remaining
21	Reads	Reads	#	Durland, Evan		Net mapped loci
22	Temperature, water	Temp	°C	Durland, Evan
23	Temperature, water, standard error	T std e	±	Durland, Evan
24	Salinity	Sal		Durland, Evan
25	Salinity, standard error	Sal std e	±	Durland, Evan
26	Alkalinity, total	AT	µmol/kg	Durland, Evan
27	Alkalinity, total, standard error	AT std e	±	Durland, Evan
28	Carbon, inorganic, dissolved	DIC	µmol/kg	Durland, Evan
29	Carbon, inorganic, dissolved, standard error	DIC std e	±	Durland, Evan
30	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Durland, Evan
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Durland, Evan
32	Bicarbonate ion	[HCO3]-	µmol/kg	Durland, Evan
33	Bicarbonate ion, standard error	[HCO3]- std e	±	Durland, Evan
34	Carbonate ion	[CO3]2-	µmol/kg	Durland, Evan
35	Carbonate ion, standard error	[CO3]2- std e	±	Durland, Evan
36	pH	pH		Durland, Evan		total scale
37	pH, standard error	pH std e	±	Durland, Evan		total scale
38	Calcite saturation state	Omega Cal		Durland, Evan
39	Calcite saturation state, standard error	Omega Cal std e	±	Durland, Evan
40	Aragonite saturation state	Omega Arg		Durland, Evan
41	Aragonite saturation state, standard error	Omega Arg std e	±	Durland, Evan
42	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1950 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Group	6 Treat	7 Age [days]	8 Repl	9 Reads [#] (Raw)	10 Reads [#] (Quality)	11 Reads [#] (matched to illumina adaptor seq)	12 Reads [#] (Quality and clean)	13 Reads [#] (Mapped)	14 Perc [%] (mapped)	15 Ratio (Avg hits/ matched read)	16 Reads [#] (Raw mappings)	17 Reads [#] (>=1 match)	18 Reads [#] (Weak match)	19 Reads [#] (Ambiguous match)	20 Reads [#] (Unique remaining)	21 Reads [#] (Net mapped loci)	22 Temp [°C]	23 T std e [±]	24 Sal	25 Sal std e [±]	26 AT [µmol/kg]	27 AT std e [±]	28 DIC [µmol/kg]	29 DIC std e [±]	30 pCO2water_SST_wet [µatm]	31 pCO2water_SST_wet std e [±]	32 [HCO3]- [µmol/kg]	33 [HCO3]- std e [±]	34 [CO3]2- [µmol/kg]	35 [CO3]2- std e [±]	36 pH (total scale)	37 pH std e [±] (total scale)	38 Omega Cal	39 Omega Cal std e [±]	40 Omega Arg	41 Omega Arg std e [±]	42 CSC flag	43 CO2 [µmol/kg]	44 fCO2water_SST_wet [µatm]	45 pCO2water_SST_wet [µatm]	46 [HCO3]- [µmol/kg]	47 [CO3]2- [µmol/kg]	48 AT [µmol/kg]	49 Omega Arg	50 Omega Cal
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	Ambient	2	1	13946577	13732377	123041	13609336	8454632	0.621237656	2.04	17209776	6976343	2504710	4153470	2822873	340824	24.5	0.86	29.8	0.27	2249	34	2069	50	651	215.1	1911	72	139.3	32.1	7.89	0.13	3.43	0.79	2.28	0.53	9	24.56	845.67	848.38	1959.72	112.86	2233.43	1.84	2.82
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	Ambient	2	2	1878491	1841248	179679	1661569	373398	0.224726147	2.08	776017	308034	107756	190341	117693	89955	24.5	0.86	29.8	0.27	2249	34	2069	50	651	215.1	1911	72	139.3	32.1	7.89	0.13	3.43	0.79	2.28	0.53	9	24.56	845.67	848.38	1959.72	112.86	2233.43	1.84	2.82
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	Ambient	2	3	5030559	4968782	12007	4956775	3258779	0.657439363	2.06	6701779	2681624	959617	1631061	1050563	333172	24.5	0.86	29.8	0.27	2249	34	2069	50	651	215.1	1911	72	139.3	32.1	7.89	0.13	3.43	0.79	2.28	0.53	9	24.56	845.67	848.38	1959.72	112.86	2233.43	1.84	2.82
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	Ambient	2	4	5145627	5092680	13813	5078867	3311382	0.651992265	2.11	6972432	2719581	981053	1731499	988082	245130	24.5	0.86	29.8	0.27	2249	34	2069	50	651	215.1	1911	72	139.3	32.1	7.89	0.13	3.43	0.79	2.28	0.53	9	24.56	845.67	848.38	1959.72	112.86	2233.43	1.84	2.82
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	Ambient	2	5	3551893	3513314	15574	3497740	1860992	0.532055556	2.13	3955306	1559796	497069	1001375	558421	161773	24.5	0.86	29.8	0.27	2249	34	2069	50	651	215.1	1911	72	139.3	32.1	7.89	0.13	3.43	0.79	2.28	0.53	9	24.56	845.67	848.38	1959.72	112.86	2233.43	1.84	2.82
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	Ambient	22	1	6372958	6283870	419610	5864260	3417879	0.582832105	2.02	6918486	2823476	1000769	1663363	1160113	261163	24.5	0.86	29.8	0.27	2249	34	2069	50	651	215.1	1911	72	139.3	32.1	7.89	0.13	3.43	0.79	2.28	0.53	9	24.56	845.67	848.38	1959.72	112.86	2233.43	1.84	2.82
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	Ambient	22	3	4322480	4281461	87145	4194316	2538489	0.605221209	2.00	5069553	2087310	758119	1201759	885551	299614	24.5	0.86	29.8	0.27	2249	34	2069	50	651	215.1	1911	72	139.3	32.1	7.89	0.13	3.43	0.79	2.28	0.53	9	24.56	845.67	848.38	1959.72	112.86	2233.43	1.84	2.82
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	Ambient	22	4	5226179	5182456	92207	5090249	3062730	0.601685694	2.02	6172169	2533891	888945	1478767	1055124	285365	24.5	0.86	29.8	0.27	2249	34	2069	50	651	215.1	1911	72	139.3	32.1	7.89	0.13	3.43	0.79	2.28	0.53	9	24.56	845.67	848.38	1959.72	112.86	2233.43	1.84	2.82
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	Ambient	22	5	5068494	5020986	45763	4975223	3161363	0.635421367	2.05	6484329	2639391	876745	1587284	1052107	296861	24.5	0.86	29.8	0.27	2249	34	2069	50	651	215.1	1911	72	139.3	32.1	7.89	0.13	3.43	0.79	2.28	0.53	9	24.56	845.67	848.38	1959.72	112.86	2233.43	1.84	2.82
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	High pCO2	2	1	5348346	5288529	22769	5265760	3262127	0.619497850	2.09	6834116	2692901	940230	1695562	997339	231149	24.4	1.02	30.5	0.26	2294	31	2260	18	1791	364.3	2146	17	61.0	10.3	7.48	0.09	1.50	0.25	1.00	0.16	9	60.88	2099.55	2106.28	2146.74	54.62	2278.54	0.89	1.36
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	High pCO2	2	2	3630852	3587004	19841	3567163	2314608	0.648865219	2.12	4906446	1935931	641148	1233186	702745	210175	24.4	1.02	30.5	0.26	2294	31	2260	18	1791	364.3	2146	17	61.0	10.3	7.48	0.09	1.50	0.25	1.00	0.16	9	60.88	2099.55	2106.28	2146.74	54.62	2278.54	0.89	1.36
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	High pCO2	2	3	3393679	3345630	101380	3244250	1772359	0.546307775	2.05	3631239	1477732	496477	887255	590477	213617	24.4	1.02	30.5	0.26	2294	31	2260	18	1791	364.3	2146	17	61.0	10.3	7.48	0.09	1.50	0.25	1.00	0.16	9	60.88	2099.55	2106.28	2146.74	54.62	2278.54	0.89	1.36
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	High pCO2	2	4	8565784	8476538	38603	8437935	5518882	0.654055998	2.11	11638519	4548491	1641276	2889090	1659401	312304	24.4	1.02	30.5	0.26	2294	31	2260	18	1791	364.3	2146	17	61.0	10.3	7.48	0.09	1.50	0.25	1.00	0.16	9	60.88	2099.55	2106.28	2146.74	54.62	2278.54	0.89	1.36
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	High pCO2	2	5	5606621	5541274	40282	5500992	3177689	0.577657448	2.14	6792579	2684745	825894	1731283	953462	186084	24.4	1.02	30.5	0.26	2294	31	2260	18	1791	364.3	2146	17	61.0	10.3	7.48	0.09	1.50	0.25	1.00	0.16	9	60.88	2099.55	2106.28	2146.74	54.62	2278.54	0.89	1.36
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	High pCO2	22	1	2929883	2904645	42745	2861900	1737413	0.607083756	1.99	3462043	1426954	520923	816501	610453	287675	24.4	1.02	30.5	0.26	2294	31	2260	18	1791	364.3	2146	17	61.0	10.3	7.48	0.09	1.50	0.25	1.00	0.16	9	60.88	2099.55	2106.28	2146.74	54.62	2278.54	0.89	1.36
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	High pCO2	22	2	6158540	6079948	36990	6042958	3761322	0.622430604	2.03	7637542	3093510	1135480	1826738	1266772	303578	24.4	1.02	30.5	0.26	2294	31	2260	18	1791	364.3	2146	17	61.0	10.3	7.48	0.09	1.50	0.25	1.00	0.16	9	60.88	2099.55	2106.28	2146.74	54.62	2278.54	0.89	1.36
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	High pCO2	22	3	5106231	5056841	125250	4931591	3081673	0.624884140	2.00	6173756	2543944	910198	1467920	1076024	278556	24.4	1.02	30.5	0.26	2294	31	2260	18	1791	364.3	2146	17	61.0	10.3	7.48	0.09	1.50	0.25	1.00	0.16	9	60.88	2099.55	2106.28	2146.74	54.62	2278.54	0.89	1.36
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	High pCO2	22	4	5474384	5425882	56657	5369225	3318855	0.618125521	1.97	6548697	2705934	1022501	1524820	1181114	369039	24.4	1.02	30.5	0.26	2294	31	2260	18	1791	364.3	2146	17	61.0	10.3	7.48	0.09	1.50	0.25	1.00	0.16	9	60.88	2099.55	2106.28	2146.74	54.62	2278.54	0.89	1.36
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	MBP	High pCO2	22	5	5453414	5385474	72529	5312945	3236995	0.609265671	2.02	6554175	2683619	936943	1579342	1104277	295178	24.4	1.02	30.5	0.26	2294	31	2260	18	1791	364.3	2146	17	61.0	10.3	7.48	0.09	1.50	0.25	1.00	0.16	9	60.88	2099.55	2106.28	2146.74	54.62	2278.54	0.89	1.36
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	Ambient	2	1	12125947	11990480	40011	11950469	7952953	0.665492961	2.11	16763388	6571723	2353809	4162912	2408811	343709	24.5	0.90	29.8	2.27	2267	30	2083	56	640	203.1	1922	77	142.0	29.7	7.89	0.12	3.50	0.73	2.33	0.48	9	23.95	824.85	827.49	1982.87	118.37	2269.08	1.93	2.95
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	Ambient	2	2	2229377	2199219	40660	2158559	1295826	0.600319936	2.10	2714863	1070686	384702	669381	401305	162203	24.5	0.90	29.8	2.27	2267	30	2083	56	640	203.1	1922	77	142.0	29.7	7.89	0.12	3.50	0.73	2.33	0.48	9	23.95	824.85	827.49	1982.87	118.37	2269.08	1.93	2.95
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	Ambient	2	3	4396436	4350110	17557	4332553	2536505	0.585452734	2.08	5265479	2089392	753353	1289728	799664	214750	24.5	0.90	29.8	2.27	2267	30	2083	56	640	203.1	1922	77	142.0	29.7	7.89	0.12	3.50	0.73	2.33	0.48	9	23.95	824.85	827.49	1982.87	118.37	2269.08	1.93	2.95
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	Ambient	2	4	2357303	2331181	8366	2322815	1421060	0.611783547	2.10	2989325	1185307	401070	744084	441223	193189	24.5	0.90	29.8	2.27	2267	30	2083	56	640	203.1	1922	77	142.0	29.7	7.89	0.12	3.50	0.73	2.33	0.48	9	23.95	824.85	827.49	1982.87	118.37	2269.08	1.93	2.95
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	Ambient	2	5	3912891	3858573	84178	3774395	1323729	0.350712896	2.13	2817945	1113036	344743	713152	399884	200158	24.5	0.90	29.8	2.27	2267	30	2083	56	640	203.1	1922	77	142.0	29.7	7.89	0.12	3.50	0.73	2.33	0.48	9	23.95	824.85	827.49	1982.87	118.37	2269.08	1.93	2.95
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	Ambient	22	1	8761300	8666230	90015	8576215	5074431	0.591686542	2.07	10494511	4250854	1397947	2595811	1655043	274894	24.5	0.90	29.8	2.27	2267	30	2083	56	640	203.1	1922	77	142.0	29.7	7.89	0.12	3.50	0.73	2.33	0.48	9	23.95	824.85	827.49	1982.87	118.37	2269.08	1.93	2.95
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	Ambient	22	2	4693999	4640630	52350	4588280	2763362	0.602265337	2.02	5576166	2288543	804410	1337684	950859	284362	24.5	0.90	29.8	2.27	2267	30	2083	56	640	203.1	1922	77	142.0	29.7	7.89	0.12	3.50	0.73	2.33	0.48	9	23.95	824.85	827.49	1982.87	118.37	2269.08	1.93	2.95
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	Ambient	22	3	8970549	8886456	50614	8835842	5614330	0.635404074	2.04	11427594	4646368	1628906	2761730	1884638	396450	24.5	0.90	29.8	2.27	2267	30	2083	56	640	203.1	1922	77	142.0	29.7	7.89	0.12	3.50	0.73	2.33	0.48	9	23.95	824.85	827.49	1982.87	118.37	2269.08	1.93	2.95
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	Ambient	22	4	5238631	5178774	81608	5097166	2864427	0.561964629	2.04	5834923	2384219	813295	1416429	967790	241909	24.5	0.90	29.8	2.27	2267	30	2083	56	640	203.1	1922	77	142.0	29.7	7.89	0.12	3.50	0.73	2.33	0.48	9	23.95	824.85	827.49	1982.87	118.37	2269.08	1.93	2.95
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	Ambient	22	5	5369862	5314699	92454	5222245	3278707	0.627834772	2.03	6670811	2715737	948032	1614616	1101121	337526	24.5	0.90	29.8	2.27	2267	30	2083	56	640	203.1	1922	77	142.0	29.7	7.89	0.12	3.50	0.73	2.33	0.48	9	23.95	824.85	827.49	1982.87	118.37	2269.08	1.93	2.95
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	High pCO2	2	1	4873652	4816995	35516	4781479	3095205	0.647332133	2.03	6291917	2549797	928002	1511424	1038373	354941	24.3	1.00	30.5	0.36	2304	23	2270	24	1790	326.8	2156	23	61.1	9.2	7.48	0.08	1.50	0.22	1.00	0.15	9	61.00	2096.76	2103.49	2167.23	54.76	2299.12	0.89	1.37
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	High pCO2	2	2	6753058	6675977	42073	6633904	4180111	0.630113279	2.03	8506196	3455492	1241448	2053659	1401833	282888	24.3	1.00	30.5	0.36	2304	23	2270	24	1790	326.8	2156	23	61.1	9.2	7.48	0.08	1.50	0.22	1.00	0.15	9	61.00	2096.76	2103.49	2167.23	54.76	2299.12	0.89	1.37
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	High pCO2	2	3	4961648	4902193	74251	4827942	2196793	0.455016444	2.17	4773860	1880854	526028	1239367	641487	146499	24.3	1.00	30.5	0.36	2304	23	2270	24	1790	326.8	2156	23	61.1	9.2	7.48	0.08	1.50	0.22	1.00	0.15	9	61.00	2096.76	2103.49	2167.23	54.76	2299.12	0.89	1.37
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	High pCO2	2	4	4476514	4429801	39608	4390193	2803751	0.638639577	2.12	5949514	2335600	794981	1492469	843131	222740	24.3	1.00	30.5	0.36	2304	23	2270	24	1790	326.8	2156	23	61.1	9.2	7.48	0.08	1.50	0.22	1.00	0.15	9	61.00	2096.76	2103.49	2167.23	54.76	2299.12	0.89	1.37
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	High pCO2	2	5	10911586	10785633	72203	10713430	6573287	0.613555789	2.06	13572912	5435690	1937025	3306478	2129212	265857	24.3	1.00	30.5	0.36	2304	23	2270	24	1790	326.8	2156	23	61.1	9.2	7.48	0.08	1.50	0.22	1.00	0.15	9	61.00	2096.76	2103.49	2167.23	54.76	2299.12	0.89	1.37
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	High pCO2	22	1	5668343	5602846	211898	5390948	2989686	0.554575188	2.12	6344654	2535571	762723	1608676	926895	179327	24.3	1.00	30.5	0.36	2304	23	2270	24	1790	326.8	2156	23	61.1	9.2	7.48	0.08	1.50	0.22	1.00	0.15	9	61.00	2096.76	2103.49	2167.23	54.76	2299.12	0.89	1.37
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	High pCO2	22	2	7069057	6994474	105142	6889332	4152001	0.602671057	2.07	8588065	3483692	1140072	2124249	1359443	252823	24.3	1.00	30.5	0.36	2304	23	2270	24	1790	326.8	2156	23	61.1	9.2	7.48	0.08	1.50	0.22	1.00	0.15	9	61.00	2096.76	2103.49	2167.23	54.76	2299.12	0.89	1.37
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	High pCO2	22	3	2176120	2153021	203352	1949669	1128344	0.578736185	2.14	2415609	951198	302426	614564	336634	216265	24.3	1.00	30.5	0.36	2304	23	2270	24	1790	326.8	2156	23	61.1	9.2	7.48	0.08	1.50	0.22	1.00	0.15	9	61.00	2096.76	2103.49	2167.23	54.76	2299.12	0.89	1.37
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	High pCO2	22	4	2757234	2728897	36753	2692144	1455484	0.540641214	2.06	2993593	1220045	398015	736278	483767	217103	24.3	1.00	30.5	0.36	2304	23	2270	24	1790	326.8	2156	23	61.1	9.2	7.48	0.08	1.50	0.22	1.00	0.15	9	61.00	2096.76	2103.49	2167.23	54.76	2299.12	0.89	1.37
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Willapa	High pCO2	22	5	4509721	4472129	73719	4398410	2680780	0.609488429	2.00	5373349	2198311	816059	1270378	927933	319052	24.3	1.00	30.5	0.36	2304	23	2270	24	1790	326.8	2156	23	61.1	9.2	7.48	0.08	1.50	0.22	1.00	0.15	9	61.00	2096.76	2103.49	2167.23	54.76	2299.12	0.89	1.37