Citation:

PANGAEA.936683

Name: Seawater carbonate chemistry and gene expression (RT-PCR) and enzyme activity of the Antarctic coral Malacobelemnon daytoni
Published: 2021-10-05
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Antarctic; Benthic animals; Benthos; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Laboratory experiment; Malacobelemnon daytoni; Other metabolic rates; Polar; Single species

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Abstract:

Benthic organisms of the Southern Ocean are particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. OA most strongly affects animals with calcium carbonate skeletons or shells, such as corals and mollusks. We exposed the abundant cold-water coral Malacobelemnon daytoni from an Antarctic fjord to low pH seawater (LpH) (7.68 +/- 0.17) to test its physiological responses to OA, at the level of gene expression (RT-PCR) and enzyme activity. Corals were exposed in short- (3 days) and long-term (54 days) experiments to two pCO2 conditions (ambient and elevated pCO2 equaling RCP 8.5, IPCC 2019, approximately 372.53 and 956.78 μatm, respectively).

Of the eleven genes studied through RT-PCR, six were significantly upregulated compared with control in the short-term in the LpH condition, including the antioxidant enzyme superoxide dismutase (SOD), Heat Shock Protein 70 (HSP70), Toll-like receptor (TLR), galaxin and ferritin. After long-term exposure to low pH conditions, RT-PCR analysis showed seven genes were upregulated. These include the mannose-binding C-Lectin and HSP90. Also, the expression of TLR and galaxin, among others, continued to be upregulated after long-term exposure to low pH. Expression of carbonic anhydrase (CA), a key enzyme involved in calcification, was also significantly upregulated after long-term exposure. Our results indicated that, after two months, M. daytoni is not acclimatized to this experimental LpH condition. Gene expression profiles revealed molecular impacts that were not evident at the enzyme activity level. Consequently, understanding the molecular mechanisms behind the physiological processes in the response of a coral to LpH is critical to understanding the ability of polar species to cope with future environmental changes. Approaches integrating molecular tools into Antarctic ecological and/or conservation research make an essential contribution given the current ongoing OA processes.

Keyword(s):

Animalia; Antarctic; Benthic animals; Benthos; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Laboratory experiment; Malacobelemnon daytoni; Other metabolic rates; Polar; Single species

Supplement to:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -62.233300 * Longitude: -58.666700

Event(s):

King_George_Island_OA * Latitude: -62.233300 * Longitude: -58.666700 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-10-05.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Servetto, Natalia		study
2	Species	Species		Servetto, Natalia
3	Registration number of species	Reg spec no		Servetto, Natalia
4	Uniform resource locator/link to reference	URL ref		Servetto, Natalia		WoRMS Aphia ID
5	Treatment	Treat		Servetto, Natalia
6	Day of experiment	DOE	day	Servetto, Natalia
7	Sample ID	Sample ID		Servetto, Natalia
8	Sample ID	Sample ID		Servetto, Natalia		Smaller Tank
9	Catalase activity, unit per protein mass	CAT/prot	U/mg	Servetto, Natalia
10	Superoxide dismutase activity, unit per protein mass	SOD/prot	U/mg	Servetto, Natalia
11	Gene expression	Gene expression		Servetto, Natalia		Ub L 40
12	Gene expression	Gene expression		Servetto, Natalia		Ub L 40
13	Gene expression	Gene expression		Servetto, Natalia		18S
14	Gene expression	Gene expression		Servetto, Natalia		18S
15	Gene expression	Gene expression		Servetto, Natalia		Ferritin
16	Gene expression	Gene expression		Servetto, Natalia		Galaxin
17	Gene expression	Gene expression		Servetto, Natalia		Perox
18	Gene expression	Gene expression		Servetto, Natalia		Metal 1
19	Gene expression	Gene expression		Servetto, Natalia		Serin Protease
20	Gene expression	Gene expression		Servetto, Natalia		SOD
21	Gene expression	Gene expression		Servetto, Natalia		TLR-2
22	Gene expression	Gene expression		Servetto, Natalia		HSP90 1
23	Gene expression	Gene expression		Servetto, Natalia		Lectin 1
24	Gene expression	Gene expression		Servetto, Natalia		Anhyd Carbonic
25	Gene expression	Gene expression		Servetto, Natalia		HSP70 2
26	Temperature, water	Temp	°C	Servetto, Natalia
27	Temperature, water, standard deviation	Temp std dev	±	Servetto, Natalia
28	Salinity	Sal		Servetto, Natalia
29	Salinity, standard deviation	Sal std dev	±	Servetto, Natalia
30	Alkalinity, total	AT	µmol/kg	Servetto, Natalia	Potentiometric titration
31	Alkalinity, total, standard deviation	AT std dev	±	Servetto, Natalia	Potentiometric titration
32	pH	pH		Servetto, Natalia	Potentiometric	total scale
33	pH, standard deviation	pH std dev	±	Servetto, Natalia	Potentiometric	total scale
34	Bicarbonate ion	[HCO3]-	µmol/kg	Servetto, Natalia	Calculated using CO2SYS
35	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Servetto, Natalia	Calculated using CO2SYS
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Servetto, Natalia	Calculated using CO2SYS
37	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Servetto, Natalia	Calculated using CO2SYS
38	Dry air column-averaged mixing ratio of carbon dioxide	XCO2	ppmv	Servetto, Natalia	Calculated using CO2SYS
39	Dry air column-averaged mixing ratio of carbon dioxide, standard deviation	XCO2 std dev	±	Servetto, Natalia	Calculated using CO2SYS
40	Carbon dioxide	CO2	µmol/kg	Servetto, Natalia	Calculated using CO2SYS
41	Carbon dioxide, standard deviation	CO2 std dev	±	Servetto, Natalia	Calculated using CO2SYS
42	Calcite saturation state	Omega Cal		Servetto, Natalia	Calculated using CO2SYS
43	Calcite saturation state, standard deviation	Omega Cal std dev	±	Servetto, Natalia	Calculated using CO2SYS
44	Aragonite saturation state	Omega Arg		Servetto, Natalia	Calculated using CO2SYS
45	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Servetto, Natalia	Calculated using CO2SYS
46	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
49	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
51	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
53	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
55	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
57	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
59	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
61	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3461 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 DOE [day]	7 Sample ID	8 Sample ID (Smaller Tank)	9 CAT/prot [U/mg]	10 SOD/prot [U/mg]	11 Gene expression (Ub L 40)	12 Gene expression (Ub L 40)	13 Gene expression (18S)	14 Gene expression (18S)	15 Gene expression (Ferritin)	16 Gene expression (Galaxin)	17 Gene expression (Perox)	18 Gene expression (Metal 1)	19 Gene expression (Serin Protease)	20 Gene expression (SOD)	21 Gene expression (TLR-2)	22 Gene expression (HSP90 1)	23 Gene expression (Lectin 1)	24 Gene expression (Anhyd Carbonic)	25 Gene expression (HSP70 2)	26 Temp [°C]	27 Temp std dev [±]	28 Sal	29 Sal std dev [±]	30 AT [µmol/kg] (Potentiometric titration)	31 AT std dev [±] (Potentiometric titration)	32 pH (total scale, Potentiometric)	33 pH std dev [±] (total scale, Potentiometric)	34 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	35 [HCO3]- std dev [±] (Calculated using CO2SYS)	36 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	37 pCO2 std dev [±] (Calculated using CO2SYS)	38 XCO2 [ppmv] (Calculated using CO2SYS)	39 XCO2 std dev [±] (Calculated using CO2SYS)	40 CO2 [µmol/kg] (Calculated using CO2SYS)	41 CO2 std dev [±] (Calculated using CO2SYS)	42 Omega Cal (Calculated using CO2SYS)	43 Omega Cal std dev [±] (Calculated using CO2SYS)	44 Omega Arg (Calculated using CO2SYS)	45 Omega Arg std dev [±] (Calculated using CO2SYS)	46 CSC flag (Calculated using seacarb afte...)	47 CO2 [µmol/kg] (Calculated using seacarb afte...)	48 CO2 std dev [±] (Calculated using seacarb afte...)	49 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	50 fCO2 std dev [±] (Calculated using seacarb afte...)	51 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	52 pCO2 std dev [±] (Calculated using seacarb afte...)	53 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	54 [HCO3]- std dev [±] (Calculated using seacarb afte...)	55 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	56 [CO3]2- std dev [±] (Calculated using seacarb afte...)	57 DIC [µmol/kg] (Calculated using seacarb afte...)	58 DIC std dev [±] (Calculated using seacarb afte...)	59 Omega Arg (Calculated using seacarb afte...)	60 Omega Arg std dev [±] (Calculated using seacarb afte...)	61 Omega Cal (Calculated using seacarb afte...)	62 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	7	1	0.00013316																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	8	1	0.00045877																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	9	2	0.00011995																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	11	3	0.00009982																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	12	3	0.00059823																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	13	1	0.00024661																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	14	1	0.00001617																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	16	2	0.00017628																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	17	3	0.00038365																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	18	3	0.00027294																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	25	1	0.00044690																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	27	2	0.00047087																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	29	3	0.00015753																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	30	3	0.00046022																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	31	1	0.00220758																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	33	2	0.00061345																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	35	3	0.00049163																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	36	3	0.00058155																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	40	2	0.00145453																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	41	2	0.00041410																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	42	3	0.00064289																	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	48	2	0.00034996																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	51	3	0.00093956																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	52	3	0.00053162																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	54	3	0.00052955																	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	1b		0.00013993
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	5		0.00021757
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	6		0.00010065
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	19		0.00053423
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	21		0.00044526
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	23		0.00036791
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	24		0.00006027
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	7	1		0.00001544																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	8	1		0.00002728																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	9	2		0.00002394																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	10	2		0.00007958																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	11	3		0.00001433																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	12	3		0.00000874																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	13	1		0.00003668																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	14	1		0.00002075																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	15	2		0.00001492																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	16	2		0.00003199																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	17	3		0.00001818																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	18	3		0.00005511																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	25	1		0.00014998																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	27	2		0.00008678																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	29	3		0.00013259																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	30	3		0.00008182																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	31	1		0.00008311																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	35	3		0.00006397																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	36	3		0.00010129																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	40	2		0.00006455																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	41	2		0.00005387																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	42	3		0.00000003																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	43	3		0.00006946																0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	48	2		0.00006342																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	51	3		0.00004086																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	52	3		0.00003511																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	54	3		0.00004333																0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	1 (Bca)			0.00004589
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	2 (Mo)			0.00006588
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	5			0.00003508
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	19			0.00007856
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	21			0.00010740
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	23			0.00004758
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	24			0.00003652
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	1				28.481		23.258		27.875	26.265	23.590	27.610	28.847	27.550	25.987	25.155	42.132	42.132	40.318
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	2				27.616		27.170		27.583	27.101	23.817	29.131	32.578	29.503	27.104	28.699	25.993	32.786	39.541
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	3				27.137		29.971		26.036	24.334	23.660	27.614	37.339	26.803	27.169	35.526	24.486	28.091	41.654
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	19				28.241		32.934		27.503	26.300	25.618	29.180	42.130	29.757	27.685	34.599	25.598	29.841	44.458
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	T0	0	21				26.816		34.221		25.453	24.183	23.182	27.511	37.951	26.688	25.249	39.825	25.384	28.736	43.120
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	7				28.115	28.306	28.496	27.757	28.222	28.358	24.445	28.044	31.976	28.677	28.677	29.134	26.624	32.652	39.707	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	9				29.341		33.586		27.219	30.904	24.720	29.069	40.151	28.282	28.051	34.772	26.876	32.754	41.521	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	3	11				28.397		29.932		26.834	28.591	24.746	28.008	34.449	28.366	27.779	31.872	26.642	32.394	39.887	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	13				28.646		28.244		27.437	28.628	24.898	29.278	32.982	28.375	28.204	29.370	26.560	33.137	39.135	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	15				30.338		35.862		28.393	30.728	26.696	31.959	38.780	30.728	29.745	40.108	28.853	35.360	40.304	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	3	17				30.217		35.159		28.126	30.527	26.992	31.687	41.179	30.937	28.186	39.640	27.434	34.526	41.474	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	25				28.085		30.282		26.912	25.750	24.989	28.124	34.993	28.968	28.996	32.047	26.508	32.247	39.268	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	27				25.194		31.394		27.671	26.992	25.092	29.007	36.001	29.830	29.584	32.590	27.283	32.947	38.998	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	29				30.019		24.367		28.306	27.998	26.841	30.436	29.131	30.288	28.045	25.581	27.678	33.323	42.531	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	41				29.842		34.263		28.313	27.331	26.660	31.115	39.717	31.112	30.893	41.561	28.835	34.292	40.392	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Control	54	42				28.922		34.140		27.736	26.542	25.964	27.853	41.143	30.394	30.758	39.710	28.086	34.358	40.561	0.87	0.86	33.55	0.16	2441.76	23.91	8.00	0.18	2223.37	62.12	372.53	44.32	374.74	44.59	23.53	2.81	2.54	0.22	1.59	0.14	8	28.47810976	12.93111952	464.4472726	210.4787680	466.4740347	211.3974914	2215.853045	88.02046117	90.79665046	34.42171526	2335.127805	67.83166098	1.37393371	0.524688094	2.190766922	0.836494049
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	33				30.110		26.727		28.011	26.320	25.751	28.543	31.478	29.975	29.147	27.921	27.993	32.594	39.740	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	35				28.755		27.081		27.560	26.603	25.763	29.362	32.003	29.562	28.503	28.751	27.366	33.245	38.433	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	51				29.675		33.183		27.175	26.644	26.153	28.464	39.279	29.880	28.203	36.427	27.460	32.989	39.847	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063
laboratory	Malacobelemnon daytoni (coral)	766545	marinespecies.org	Elevated pCO2	54	52				29.147		26.115		27.232	28.748	26.254	29.008	30.695	30.409	30.109	27.578	28.278	33.154	40.846	0.87	0.86	33.55	0.16	2450.60	31.70	7.69	0.17	2447.94	324.27	956.78	172.08	962.47	173.10	60.41	10.84	1.33	0.28	0.83	0.18	8	61.24400634	25.20457103	998.8237262	410.2008567	1003.1848260	411.9930583	2333.959888	53.28527954	46.84057964	17.61530349	2442.044474	60.37476919	0.70879103	0.268540302	1.130182578	0.428121063