Citation:

PANGAEA.934302

Name: Seawater carbonate chemistry and microbial abundances, bacterial activity and extracellular enzyme activities
Published: 2016
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Biomass/Abundance/Elemental composition; Coast and continental shelf; Community composition and diversity; Containers and aquaria (20-1000 L or < 1 m**2); Entire community; Laboratory experiment; Macro-nutrients; Mediterranean Sea; Other metabolic rates; Pelagos; Temperate; Temperature

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Abstract:

We investigated the effects of an increase in dissolved CO2 on the microbial communities of the Mediterranean Sea during two mesocosm experiments in two contrasting seasons: winter, at the peak of the annual phytoplankton bloom, and summer, under low nutrient conditions. The experiments included treatments with acidification and nutrient addition, and combinations of the two. We followed the effects of ocean acidification (OA) on the abundance of the main groups of microorganisms (diatoms, dinoflagellates, nanoeukaryotes, picoeukaryotes, cyanobacteria, and heterotrophic bacteria) and on bacterial activity, leucine incorporation, and extracellular enzyme activity. Our results showed a clear stimulation effect of OA on the abundance of small phytoplankton (pico- and nanoeukaryotes), independently of the season and nutrient availability. A large number of the measured variables showed significant positive effects of acidification in summer compared with winter, when the effects were sometimes negative. Effects of OA were more conspicuous when nutrient concentrations were low. Our results therefore suggest that microbial communities in oligotrophic waters are considerably affected by OA, whereas microbes in more productive waters are less affected. The overall enhancing effect of acidification on eukaryotic pico- and nanophytoplankton, in comparison with the non-significant or even negative response to nutrient-rich conditions of larger groups and autotrophic prokaryotes, suggests a shift towards medium-sized producers in a future acidified ocean.

Keyword(s):

Biomass/Abundance/Elemental composition; Coast and continental shelf; Community composition and diversity; Containers and aquaria (20-1000 L or < 1 m**2); Entire community; Laboratory experiment; Macro-nutrients; Mediterranean Sea; Other metabolic rates; Pelagos; Temperate; Temperature

Supplement to:

Sala, M M; Aparicio, F L; Balagué, Vanessa; Boras, J A; Borrull, E; Cardelus, C; Cros, Lluisa; Gomes, Ana; Lopez-Sanz, Angel; Malits, A; Martinez, R A; Mestre, M; Movilla, Juancho; Sarmento, Hugo; Vazquez-Dominguez, E; Vaqué, Dolors; Pinhassi, Jarone; Calbet, Albert; Calvo, Eva; Gasol, Josep M; Pelejero, Carles; Marrasé, Celia (2016): Contrasting effects of ocean acidification on the microbial food web under different trophic conditions. ICES Journal of Marine Science, 73(3), 670-679, https://doi.org/10.1093/icesjms/fsv130

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 41.666600 * Longitude: 2.800000

Event(s):

Blanes_Bay_Microbial_Observatory * Latitude: 41.666600 * Longitude: 2.800000 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-07-28.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Sala, M M		study
2	Treatment	Treat		Sala, M M
3	Replicate	Repl		Sala, M M
4	Experiment day	Exp day	day	Sala, M M
5	Alkalinity, total	AT	µmol/kg	Sala, M M	Potentiometric titration
6	Salinity	Sal		Sala, M M
7	Temperature, water	Temp	°C	Sala, M M
8	pH	pH		Sala, M M	Spectrophotometric	total scale
9	Chlorophyll a	Chl a	µg/l	Sala, M M
10	Chlorophyll a	Chl a	µg/l	Sala, M M		<3
11	Abundance per volume	Abund v	#/ml	Sala, M M		Diatoms
12	Abundance per volume	Abund v	#/ml	Sala, M M		Dinoflagellates
13	Abundance per volume	Abund v	#/ml	Sala, M M		Nanoeukaryotes
14	Abundance per volume	Abund v	#/ml	Sala, M M		Large picoeukaryotes
15	Abundance per volume	Abund v	#/ml	Sala, M M		Small picoeukaryotes
16	Abundance per volume	Abund v	#/ml	Sala, M M		Synechococcus
17	Abundance per volume	Abund v	#/ml	Sala, M M		Prochlorococcus
18	Abundance per volume	Abund v	#/ml	Sala, M M		Bacteria
19	Leucine incorporation rate	Leuc inc rate	nmol/l/h	Sala, M M
20	Chitobiase activity	Chitobiase activity	µmol/l/h	Sala, M M
21	alpha-glucosidase activity	MUF-aglu	µmol/l/h	Sala, M M
22	beta-glucosidase activity	MUF-bglu	µmol/l/h	Sala, M M
23	Leucine aminopeptidase activity	Leu aminopep	nmol/l/h	Sala, M M
24	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
25	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3914 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Treat	3 Repl	4 Exp day [day]	5 AT [µmol/kg] (Potentiometric titration)	6 Sal	7 Temp [°C]	8 pH (total scale, Spectrophotometric)	9 Chl a [µg/l]	10 Chl a [µg/l] (<3)	11 Abund v [#/ml] (Diatoms)	12 Abund v [#/ml] (Dinoflagellates)	13 Abund v [#/ml] (Nanoeukaryotes)	14 Abund v [#/ml] (Large picoeukaryotes)	15 Abund v [#/ml] (Small picoeukaryotes)	16 Abund v [#/ml] (Synechococcus)	17 Abund v [#/ml] (Prochlorococcus)	18 Abund v [#/ml] (Bacteria)	19 Leuc inc rate [nmol/l/h]	20 Chitobiase activity [µmol/l/h]	21 MUF-aglu [µmol/l/h]	22 MUF-bglu [µmol/l/h]	23 Leu aminopep [nmol/l/h]	24 CSC flag (Calculated using seacarb afte...)	25 CO2 [µmol/kg] (Calculated using seacarb afte...)	26 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	27 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	28 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	29 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	30 DIC [µmol/kg] (Calculated using seacarb afte...)	31 Omega Arg (Calculated using seacarb afte...)	32 Omega Cal (Calculated using seacarb afte...)
laboratory	winter, nutrients added, acidified	1	0	2534	37.92	15.0	8.06	746.60	345.22			173	1316	3400	2401	1436	495499	7.0	0.00000	0.00000	0.00000	0.57	8	15.38	417.24	418.75	2070.58	188.45	2274.41	2.84	4.40
laboratory	winter, nutrients added, acidified	1	1	2547	37.92	15.0	7.99	560.97	252.75	7.1500	0.8000	85	1351	4788	3063	1714	544978	44.0					8	18.67	506.60	508.44	2139.81	165.76	2324.25	2.49	3.87
laboratory	winter, nutrients added, acidified	1	2		37.92	15.3	7.85	964.26	462.34	22.5000	0.5000	134	2936	9654	4675	2310	2279620	575.3	0.00001	0.00003	0.01467	7.22
laboratory	winter, nutrients added, acidified	1	3		37.92	15.6	7.78	2034.30	808.42	48.2000	0.6000	278	5353	27672	6430	2880	3658502	777.0
laboratory	winter, nutrients added, acidified	1	4		37.92	15.6	7.80	5116.58	1061.66	206.7000	1.3000	835	10126	43791	8700	3697	4221224	393.1	0.00025	0.00010	0.08799	10.11
laboratory	winter, nutrients added, acidified	1	5	2550	37.92	15.7	7.83	10519.20	1100.62	854.1950	3.3550	2069	16152	28978	10581	4388	4191618	364.2	0.00079	0.00005	0.07215	14.96	8	27.87	772.41	775.19	2246.58	123.68	2398.13	1.86	2.89
laboratory	winter, nutrients added, acidified	1	6		37.92	15.6	7.93	20959.49	749.98	1981.9300	2.5300	2510	15692	8918	6275	3464	2776464	184.0	0.00220	0.00011	0.14429	12.95
laboratory	winter, nutrients added, acidified	1	7		37.92	15.6	8.00	25891.14	457.78	2523.7700	3.0000	1561	9650	1630	2848	1610	1282241	140.1	0.00332	0.00009	0.08511	23.17
laboratory	winter, nutrients added, acidified	1	8		37.92	15.6	8.07	17877.22	487.00	4931.1000	4.4900	1653	7556	819	3820		1973886	329.8	0.00433	0.00010	0.32932	30.31
laboratory	winter, nutrients added, acidified	1	9	2517	37.92	15.6	8.10	16027.85	3205.57	5189.5000	5.4450	989	4796	592	5242	1926	3124576	499.6	0.00360	0.00020	0.46359	27.26	8	13.43	371.16	372.49	2011.50	205.41	2230.35	3.09	4.80
laboratory	winter, nutrients added, acidified	2	0	2534	37.92	15.0	8.06	746.60	345.22			173	1316	3400	2401	1436	511400	7.0	0.00000	0.00000	0.00000	0.57	8	15.38	417.24	418.75	2070.58	188.45	2274.41	2.84	4.40
laboratory	winter, nutrients added, acidified	2	1	2547	37.92	14.9	7.93	517.82	215.76			87	1989	3396	3156	1815	519732	36.4					8	21.95	593.83	595.98	2186.06	146.93	2354.94	2.21	3.43
laboratory	winter, nutrients added, acidified	2	2		37.92	15.1	7.85	788.94	326.72			103	2581	6205	3856	2090	1870974	584.2	0.00001	0.00002	0.01077	5.05
laboratory	winter, nutrients added, acidified	2	3		37.92	15.4	7.78	1470.74	457.78			116	4262	20246	4565	2513	3110939	786.8
laboratory	winter, nutrients added, acidified	2	4		37.92	15.4	7.80	4068.61	944.78			529	7223	41395	5511	2441	2949067	323.8	0.00026	0.00004	0.07357	8.44
laboratory	winter, nutrients added, acidified	2	5	2557	37.92	15.4	7.83	10714.00	1373.34			1215	15036	42850	6257	2817	3731200	244.7	0.00069	0.00008	0.06284	12.22	8	28.18	774.05	776.84	2255.85	122.77	2406.80	1.85	2.87
laboratory	winter, nutrients added, acidified	2	6		37.92	15.5	7.91	20959.49	1256.46			1444	15343	16497	3011	1798	2720329	226.1	0.00282	0.00010	0.12514	9.64
laboratory	winter, nutrients added, acidified	2	7		37.92	15.5	8.00	28356.96	847.38			2257	18879	4516	1675	0	1461626	141.1	0.00373	0.00005	0.06880	23.40
laboratory	winter, nutrients added, acidified	2	8		37.92	15.7	8.08	22808.86	511.66			1011	9678	2435	2298		2530423	292.7	0.00475	0.00009	0.37468	36.11
laboratory	winter, nutrients added, acidified	2	9	2520	37.92	15.6	8.11	14178.48	5844.00			909	7646	1614	2169	0	4586943	333.8	0.00468	0.00017	0.49413	48.91	8	13.08	361.47	362.77	2004.61	209.48	2227.17	3.16	4.89
laboratory	winter, nutrients added, not acidified	1	0	2534	37.92	15.0	8.06	746.60	345.22			173	1316	3400	2401	1436	495499	7.0	0.00000	0.00000	0.00000	0.57	8	15.38	417.24	418.75	2070.58	188.45	2274.41	2.84	4.40
laboratory	winter, nutrients added, not acidified	1	1	2542	37.92	15.0	8.06	633.10	209.59	28.9750	0.5000	118	1617	3625	3103	1819	541835	34.3					8	15.43	418.60	420.12	2077.34	189.07	2281.84	2.84	4.42
laboratory	winter, nutrients added, not acidified	1	2		37.92	14.8	8.02	711.02	252.75	20.7000	0.9000	89	2067	5166	4295	2539	2044575	605.7	0.00001	0.00004	0.00943	6.69
laboratory	winter, nutrients added, not acidified	1	3		37.92	14.9	8.00	1295.42	394.53	63.0000	1.2000	155	3284	14273	6385	2799	3478729	768.3
laboratory	winter, nutrients added, not acidified	1	4		37.92	14.9	7.98	3328.86	691.54	231.6500	1.8200	406	4512	30003	9809	3011	4172354	489.1	0.00027	0.00007	0.08998	9.92
laboratory	winter, nutrients added, not acidified	1	5	2561	37.92	14.9	7.98	8766.00	1042.18	607.1550	3.0400	788	8992	35672	15902	3804	4585219	313.3	0.00053	0.00011	0.08076	14.15	8	19.34	523.19	525.09	2161.05	162.97	2343.36	2.45	3.81
laboratory	winter, nutrients added, not acidified	1	6		37.92	15.0	8.02	19726.58	1178.54	2167.1050	8.8500	983	11982	31355	15173	3407	3877885	281.3	0.00191	0.00015	0.12121	17.24
laboratory	winter, nutrients added, not acidified	1	7		37.92	14.9	8.17	30822.78	672.06	2544.9450	14.5700	874	8847	5321	5299	0	505537	116.6	0.00332	0.00003	0.07374	18.75
laboratory	winter, nutrients added, not acidified	1	8		37.92	14.9	8.25	28973.42	289.73	3270.9900	13.6200	564	4929	1121	4004		775948	280.1	0.00380	0.00007	0.20630	31.93
laboratory	winter, nutrients added, not acidified	1	9	2532	37.92	14.9	8.24	25274.68	357.54	5055.7050	18.3000	466	2741	842	5434	0	1082253	455.4	0.00424	0.00015	0.47542	57.34	8	9.31	251.83	252.75	1892.85	259.75	2161.92	3.91	6.07
laboratory	winter, nutrients added, not acidified	2	0	2534	37.92	15.0	8.06	746.60	345.22			173	1316	3400	2401	1436	511400	7.0	0.00000	0.00000	0.00000	0.57	8	15.38	417.24	418.75	2070.58	188.45	2274.41	2.84	4.40
laboratory	winter, nutrients added, not acidified	2	1	2543	37.92	14.8	8.07	555.18	228.09			76	1654	3404	3125	1980	521548	39.3					8	15.11	407.52	409.00	2072.24	191.52	2278.87	2.88	4.47
laboratory	winter, nutrients added, not acidified	2	2		37.92	15.0	8.04	613.62	246.58			76	2473	4089	4510	2277	1882330	525.9	0.00000	0.00002	0.01057	5.88
laboratory	winter, nutrients added, not acidified	2	3		37.92	15.3	8.00	1217.50	376.04			155	3845	10669	7257	2766	3335267	687.6
laboratory	winter, nutrients added, not acidified	2	4		37.92	15.3	7.99	3082.28	613.62			423	5801	26377	12663	3221	3992487	265.7	0.00023	0.00006	0.07970	9.93
laboratory	winter, nutrients added, not acidified	2	5	2560	37.92	15.4	7.98	9740.00	1003.22			880	10906	38413	20944	4235	4200368	225.9	0.00063	0.00009	0.06657	13.81	8	19.05	523.21	525.10	2153.86	165.58	2338.49	2.49	3.87
laboratory	winter, nutrients added, not acidified	2	6		37.92	15.3	8.05	22192.41	1295.42			737	11643	24298	15684	2827	3345406	284.7	0.00227	0.00012	0.12329	15.40
laboratory	winter, nutrients added, not acidified	2	7		37.92	15.3	8.22	28356.96	691.54			1426	13737	7993	8804	1622	954941	140.5	0.00356	0.00005	0.13838	21.46
laboratory	winter, nutrients added, not acidified	2	8		37.92	15.3	8.33	22808.86	191.10			788	6767	3961	10877		1303460	293.0	0.00428	0.00009	0.23781	33.66
laboratory	winter, nutrients added, not acidified	2	9	2515	37.92	15.3	8.33	20343.04	720.76			848	5664	2794	14481	0	2661623	340.7	0.00436	0.00014	0.37555	38.43	8	7.00	191.73	192.42	1768.16	303.14	2078.30	4.56	7.08
laboratory	winter, no nutrients added, acidified	1	0	2534	37.92	15.0	8.06	746.60	345.22			173	1316	3400	2401	1436	495499	7.0	0.00000	0.00000	0.00000	0.57	8	15.38	417.24	418.75	2070.58	188.45	2274.41	2.84	4.40
laboratory	winter, no nutrients added, acidified	1	1	2525	37.92	15.1	7.94	672.06	265.08	4.6000	1.4000	192	1782	3722	3222	1634	551275	45.3					8	21.07	573.42	575.50	2157.19	149.51	2327.77	2.25	3.49
laboratory	winter, no nutrients added, acidified	1	2		37.92	15.4	7.82	925.30	382.20	13.9000	1.3000	254	2771	8765	4305	2527	2065341	570.9	0.00001	0.00003	0.01496	5.00
laboratory	winter, no nutrients added, acidified	1	3		37.92	15.8	7.78	1334.38	633.10	39.8000	0.9000	227	3845	21516	3451	2671	3481804	635.3
laboratory	winter, no nutrients added, acidified	1	4		37.92	15.7	7.80	1587.62	672.06	66.4000	2.6000	337	2905	19075	2290	2120	3481879	176.4	0.00007	0.00008	0.05667	8.14
laboratory	winter, no nutrients added, acidified	1	5	2542	37.92	15.7	7.76	1568.14	457.78	78.4950	1.9800	576	2261	10332	1934	2004	2733611	177.0	0.00007	0.00001	0.02338	6.35	8	33.23	920.93	924.24	2279.79	106.82	2419.85	1.61	2.49
laboratory	winter, no nutrients added, acidified	1	6		37.92	15.8	7.76	1412.30	425.35	55.6000	2.9000	374	1928	2388	887	690	1241854	74.6	0.00019	0.00002	0.03858	3.08
laboratory	winter, no nutrients added, acidified	1	7		37.92	15.7	7.78	1782.42	394.53	131.5000	5.0000	768	1798	478	255	210	804771	25.4	0.00027	0.00000	0.02150	3.06
laboratory	winter, no nutrients added, acidified	1	8		37.92	15.7	7.78	2157.59	184.94	176.7000	4.3000	396	1293	364	206		977311	67.7	0.00040	0.00002	0.08225	3.98
laboratory	winter, no nutrients added, acidified	1	9	2556	37.92	15.7	7.77	2465.82	314.39	219.9000	5.5000	276	1072	476	353	0	1143039	144.2	0.00044	0.00005	0.13218	4.12	8	32.58	902.85	906.09	2287.10	109.66	2429.35	1.65	2.56
laboratory	winter, no nutrients added, acidified	2	0	2534	37.92	15.0	8.06	746.60	345.22			173	1316	3400	2401	1436	511400	7.0	0.00000	0.00000	0.00000	0.57	8	15.38	417.24	418.75	2070.58	188.45	2274.41	2.84	4.40
laboratory	winter, no nutrients added, acidified	2	1	2527	37.92	14.5	7.88	594.14	246.58			47	970	2676	2232	1122	549744	40.8					8	25.10	670.73	673.18	2206.62	130.16	2361.88	1.96	3.04
laboratory	winter, no nutrients added, acidified	2	2		37.92	15.1	7.84	730.50	320.56			107	2593	5727	4153	2387	2192285	615.2	0.00002	0.00003	0.00734	7.11
laboratory	winter, no nutrients added, acidified	2	3		37.92	15.4	7.78	1042.18	477.26			456	3612	12889	3874	2645	3679085	678.7
laboratory	winter, no nutrients added, acidified	2	4		37.92	15.4	7.78	1217.50	535.70			394	3840	15087	2845	2625	3987517	307.6	0.00006	0.00007	0.06533	9.87
laboratory	winter, no nutrients added, acidified	2	5	2544	37.92	15.5	7.76	1022.70	438.30			196	2474	12887	2374	2496	3661906	213.6	0.00004	0.00002	0.03888	8.63	8	33.44	921.14	924.46	2283.36	106.17	2422.98	1.60	2.48
laboratory	winter, no nutrients added, acidified	2	6		37.92	15.5	7.76	1178.54	351.38			206	1348	6434	1365	1244	1916352	119.2	0.00016	0.00005	0.04708	4.87
laboratory	winter, no nutrients added, acidified	2	7		37.92	15.5	7.77	1373.34	394.53			198	1107	1898	380	204	495688	25.1	0.00020	0.00000	0.00939	3.28
laboratory	winter, no nutrients added, acidified	2	8		37.92	15.6	7.78	1787.72	314.39			415	1166	1489	270		584115	108.7	0.00027	0.00001	0.04189	6.33
laboratory	winter, no nutrients added, acidified	2	9	2556	37.92	15.5	7.76	2527.47	363.71			429	1579	1650	380	253	951969	109.3	0.00036	0.00004	0.09962	3.92	8	33.60	925.56	928.90	2294.34	106.68	2434.63	1.61	2.49
laboratory	winter, no nutrients added, no acidified	1	0	2534	37.92	15.0	8.06	746.60	345.22			173	1316	3400	2401	1436	495499	7.0	0.00000	0.00000	0.00000	0.57	8	15.38	417.24	418.75	2070.58	188.45	2274.41	2.84	4.40
laboratory	winter, no nutrients added, no acidified	1	1	2527	37.92	14.9	8.02	652.58	258.91	2.4000	0.5000	130	1512	4415	3169	1675	540923	55.9					8	17.14	463.63	465.31	2099.78	173.63	2290.55	2.61	4.05
laboratory	winter, no nutrients added, no acidified	1	2		37.92	15.1	8.01	730.50	308.23	8.3000	0.6000	91	2385	7041	4743	2494	1910292	562.8	0.00001	0.00002	0.01788	5.69
laboratory	winter, no nutrients added, no acidified	1	3		37.92	15.4	7.97	1003.22	326.72	26.7000	1.1000	252	3051	16258	6566	2880	3441109	617.6
laboratory	winter, no nutrients added, no acidified	1	4		37.92	15.3	7.94	1392.82	535.70	33.1000	2.5000	217	3452	16632	7791	3101	3842931	268.6	0.00005	0.00000	0.05950	8.10
laboratory	winter, no nutrients added, no acidified	1	5	2549	37.92	15.3	7.89	1139.58	457.78	61.5650	2.9800	208	2086	13126	8876	3283	3414253	223.7	0.00005	0.00000	0.03418	7.22	8	24.12	660.36	662.74	2211.12	137.63	2372.87	2.07	3.21
laboratory	winter, no nutrients added, no acidified	1	6		37.92	15.4	7.87	1139.58	258.91	56.6000	1.1000	235	1663	5521	5881	2090	1831056	110.2	0.00020	0.00006	0.05035	3.09
laboratory	winter, no nutrients added, no acidified	1	7		37.92	15.3	7.88	1178.54	147.95	74.9000	2.6000	180	1087	1001	1367	331	2446369	17.7	0.00022	0.00000	0.08231	2.80
laboratory	winter, no nutrients added, no acidified	1	8		37.92	15.3	7.86	1236.98	394.53	122.4000	2.6000	106	513	404	456		1232219	96.1	0.00034	0.00002	0.06060	3.25
laboratory	winter, no nutrients added, no acidified	1	9	2566	37.92	15.3	7.83	2157.59	117.13	218.2000	6.2000	200	725	603	1005	323	1993252	152.8	0.00050	0.00006	0.12000	2.84	8	28.36	776.65	779.46	2264.95	122.79	2416.11	1.85	2.87
laboratory	winter, no nutrients added, no acidified	2	0	2534	37.92	15.0	8.06	746.60	345.22			173	1316	3400	2401	1436	511400	7.0	0.00000	0.00000	0.00000	0.57	8	15.38	417.24	418.75	2070.58	188.45	2274.41	2.84	4.40
laboratory	winter, no nutrients added, no acidified	2	1	2526	37.92	14.7	8.03	633.10	265.08			68	1194	4146	3183	1646	556030	44.1					8	16.78	451.08	452.72	2093.37	175.77	2285.91	2.64	4.11
laboratory	winter, no nutrients added, no acidified	2	2		37.92	14.7	8.03	613.62	277.41			76	2364	5040	3847	2063	2235645	665.0	0.00002	0.00005	0.02930	10.07
laboratory	winter, no nutrients added, no acidified	2	3		37.92	14.9	7.98	886.34	351.38			144	3113	9842	3282	2354	3764514	740.1
laboratory	winter, no nutrients added, no acidified	2	4		37.92	14.9	7.96	964.26	457.78			174	3407	13190	2564	2063	4168898	241.6	0.00006	0.00007	0.09639	11.20
laboratory	winter, no nutrients added, no acidified	2	5	2538	37.92	14.9	7.91	1081.14	379.86			141	3166	12154	2110	1816	3941283	272.3	0.00002	0.00006	0.04479	10.81	8	23.05	623.54	625.80	2192.12	140.71	2355.88	2.12	3.29
laboratory	winter, no nutrients added, no acidified	2	6		37.92	14.9	7.89	1061.66	332.89			163	1720	9161	1516	1273	2948054	196.9	0.00012	0.00009	0.05582	5.84
laboratory	winter, no nutrients added, no acidified	2	7		37.92	14.8	7.90	1042.18	221.92			186	1365	3148	601	347	479175	41.6	0.00013	0.00000	0.00823	3.41
laboratory	winter, no nutrients added, no acidified	2	8		37.92	14.9	7.87	1022.70	413.03			141	1064	2165	486		675921	115.4	0.00026	0.00000	0.03633	4.26
laboratory	winter, no nutrients added, no acidified	2	9	2552	37.92	14.8	7.84	2095.95	271.24			294	1608	2098	253	231	1382166	145.4	0.00046	0.00006	0.09961	5.45	8	27.87	751.81	754.54	2251.14	122.51	2401.53	1.84	2.86
laboratory	summer, nutrients added, acidified	1	0	2541	37.83	22.0	8.08	327.73	35.03	0.7000	2.7150	148	551	1990	11807	1507	855480	926.1	0.00009	0.00007	0.09932	3.10	8	11.87	392.58	393.88	1950.70	241.76	2204.33	3.71	5.67
laboratory	summer, nutrients added, acidified	1	1	2535	37.83	22.0	7.87	499.54	335.45	0.3000	1.1600	162	1340	7457	4780	1036	677000	1009.0	0.00010	0.00012	0.34946	3.92	8	21.08	697.40	699.72	2136.73	163.28	2321.10	2.51	3.83
laboratory	summer, nutrients added, acidified	1	2		37.83	22.0	7.77	1011.27	192.78	0.4800	1.6600						442000	492.2	0.00010	0.00003	0.11149	3.10
laboratory	summer, nutrients added, acidified	1	3		37.83	21.6	7.68	1542.28	731.04	2.5760	0.9200	562	4571	9017	2864	474	427000	657.7	0.00015	0.00006	0.26905	5.52
laboratory	summer, nutrients added, acidified	1	4		37.83	21.6	7.70	2197.75	901.62	14.0450	2.2580	967	6731	9639	4155	589	688000	646.2	0.00029	0.00034	0.50093	12.87
laboratory	summer, nutrients added, acidified	1	5		37.83	21.8	7.58	3084.56	1349.49	47.7370	3.4750	1485	8115	10031	6728	967	995000	693.6	0.00018	0.00021	0.31375	0.10
laboratory	summer, nutrients added, acidified	1	6	2546	37.83	21.9	7.66	3547.24	1696.51	39.1910	2.8650	1880	9066	6909	11209	1938	1350000	650.7	0.00023	0.00024	0.21859	14.11	8	36.64	1208.71	1212.74	2284.83	107.26	2428.73	1.65	2.51
laboratory	summer, nutrients added, acidified	1	7		37.83	21.5	7.66	2853.22	1002.48	196.6820	9.5150	1409	8455	5007	16271	3001	1530000	567.6	0.00020	0.00020	0.30254	0.06
laboratory	summer, nutrients added, acidified	1	8		37.83	21.5	7.65	1850.73	791.96	87.3310	11.4110	787	7888	2867	22873	4138	1310000	631.2	0.00020	0.00014	0.25448	0.12
laboratory	summer, nutrients added, acidified	1	9		37.83	21.3	7.56	1542.28	269.90	171.9970	20.9330	452	6002	1683	28196	4188	1300000	381.1	0.00024	0.00009	0.21746	0.16
laboratory	summer, nutrients added, acidified	2	0	2541	37.83	22.0	8.08	303.64	33.51	0.4000	2.2400	132	556	2401	13761	1538	833430	1074.0	0.00009	0.00008	0.11823	3.46	8	11.87	392.58	393.88	1950.70	241.76	2204.33	3.71	5.67
laboratory	summer, nutrients added, acidified	2	1	2539	37.83	22.0	7.87	377.70	285.32	0.3200	1.3000	132	924	6583	2601	1305	541000	1157.3	0.00009	0.00013	0.33797	3.48	8	21.12	698.53	700.85	2140.20	163.55	2324.87	2.51	3.83
laboratory	summer, nutrients added, acidified	2	2		37.83	22.0	7.74	865.06	192.78	0.2400	1.6000	195	1740	14953	2001	1063	440000	459.7	0.00009	0.00004	0.09228	2.50
laboratory	summer, nutrients added, acidified	2	3		37.83	22.0	7.67	1465.16	901.62	4.5920	1.7600	477	4703	18151	2061	458	414000	521.7	0.00014	0.00004	0.30166	4.60
laboratory	summer, nutrients added, acidified	2	4		37.83	22.0	7.69	2313.42	1233.82	6.1790	1.1350	850	6863	26908	2842	515	565000	623.0	0.00023	0.00033	0.47935	9.05
laboratory	summer, nutrients added, acidified	2	5		37.83	22.3	7.57	3354.46	1850.73	8.9440	1.8050	1510	7882	25093	4300	858	988000	682.5	0.00018	0.00021	0.30024	0.09
laboratory	summer, nutrients added, acidified	2	6	2551	37.83	22.2	7.65	3662.91	1889.29	37.3920	3.1760	2212	8351	11823	7359	1524	1080000	614.2	0.00020	0.00019	0.22473	11.44	8	37.38	1243.13	1247.25	2292.25	106.34	2435.97	1.63	2.49
laboratory	summer, nutrients added, acidified	2	7		37.83	22.3	7.62	3238.78	1580.84	20.3530	4.8430	1943	6819	7071	10579	2291	1540000	546.6	0.00017	0.00012	0.23273	0.03
laboratory	summer, nutrients added, acidified	2	8		37.83	21.8	7.60	1927.85	755.41	18.1970	6.4900	798	5553	5021	14421	2721	1360000	705.3	0.00020	0.00011	0.23629	0.13
laboratory	summer, nutrients added, acidified	2	9		37.83	21.7	7.51	1310.94	523.91	41.7240	14.3250	529	3431	3237	19686	2439	1250000	386.9	0.00030	0.00009	0.22707	0.18
laboratory	summer, nutrients added, no acidified	1	0	2541	37.83	22.0	8.07	313.28	38.08	0.5600	2.4800	101	893	2617	13629	1655	729350	788.9	0.00011	0.00005	0.08310	3.23	8	12.21	403.86	405.20	1961.11	237.51	2210.84	3.65	5.57
laboratory	summer, nutrients added, no acidified	1	1	2547	37.83	22.0	8.03	450.81	258.33	0.2600	1.1200	170	1436	6583	5363	1050	697000	1097.7	0.00010	0.00011	0.28029	3.81	8	13.70	453.04	454.55	2006.35	221.61	2241.66	3.40	5.19
laboratory	summer, nutrients added, no acidified	1	2		37.83	22.0	8.04	865.06	165.79	0.3200	1.3400	181	2179	12067	2160	748	411000	736.6	0.00011	0.00007	0.16270	3.09
laboratory	summer, nutrients added, no acidified	1	3		37.83	20.3	7.99	1156.71	682.30	1.7180	1.0800	436	3826	8526	2201	584	397000	361.4	0.00012	0.00006	0.19795	3.88
laboratory	summer, nutrients added, no acidified	1	4		37.83	20.4	7.96	1619.39	804.14	7.3770	2.8640	570	4349	7076	3078	417	585000	546.9	0.00022	0.00029	0.40730	7.48
laboratory	summer, nutrients added, no acidified	1	5		37.83	20.7	7.94	2313.42	1233.82	24.5230	4.7750	904	5747	9444	4766	603	968000	613.6	0.00018	0.00018	0.25511	0.02
laboratory	summer, nutrients added, no acidified	1	6	2555	37.83	20.7	7.93	2621.87	1426.61	33.0720	2.0450	1642	6879	8962	9080	866	1110000	723.6	0.00022	0.00015	0.41864	10.99	8	18.74	598.55	600.57	2122.30	177.39	2318.43	2.71	4.15
laboratory	summer, nutrients added, no acidified	1	7		37.83	20.2	7.91	2197.75	597.02	62.0970	3.6160	959	7052	6534	14205	1513	1440000	733.0	0.00022	0.00022	0.30785	0.11
laboratory	summer, nutrients added, no acidified	1	8		37.83	20.2	7.89	1812.18	262.19	53.3330	7.3790	847	6282	4448	17869	2598	1520000	558.0	0.00023	0.00029	0.41654	0.10
laboratory	summer, nutrients added, no acidified	1	9		37.83	20.1	7.88	1310.94	146.52	55.1400	11.1220	554	4174	3571	16948	2801	1470000	540.3	0.00028	0.00029	0.40257	0.06
laboratory	summer, nutrients added, no acidified	2	0	2541	37.83	22.0	8.07	322.91	39.60	0.5600	1.5800	60	584	2491	12053	1727	732040	1082.8	0.00010	0.00010	0.12090	3.80	8	12.21	403.86	405.20	1961.11	237.51	2210.84	3.65	5.57
laboratory	summer, nutrients added, no acidified	2	1	2534	37.83	22.0	8.03	450.81	293.03	0.4600	1.2400	85	1321	7364	3305	1028	720000	970.6	0.00009	0.00010	0.27382	4.04	8	13.62	450.64	452.13	1995.70	220.44	2229.76	3.39	5.17
laboratory	summer, nutrients added, no acidified	2	2		37.83	22.0	8.01	925.98	208.21	0.1800	0.8200	175	2398	14059	2031	559	424000	596.6	0.00009	0.00006	0.14830	2.94
laboratory	summer, nutrients added, no acidified	2	3		37.83	21.6	7.96	1349.49	901.62	3.5140	1.1800	425	4306	13506	2469	384	393000	336.3	0.00012	0.00005	0.18228	3.87
laboratory	summer, nutrients added, no acidified	2	4		37.83	21.6	7.96	2004.96	1072.19	4.4320	0.8750	737	5257	15435	3905	384	580000	604.3	0.00024	0.00030	0.40027	9.01
laboratory	summer, nutrients added, no acidified	2	5		37.83	21.8	7.94	2814.66	1503.72	8.3150	1.5100	1033	6747	16808	7115	1584	837000	631.2	0.00017	0.00019	0.25071	0.02
laboratory	summer, nutrients added, no acidified	2	6	2557	37.83	21.9	7.92	2621.87	1195.27	85.1550	3.5650	959	7797	10530	13594	1263	913000	653.0	0.00019	0.00014	0.32416	10.23	8	18.65	615.31	617.36	2116.54	180.80	2315.99	2.78	4.24
laboratory	summer, nutrients added, no acidified	2	7		37.83	21.9	7.92	1696.51	670.12	39.6280	5.2920	504	7106	7071	23841	2672	1350000	694.1	0.00017	0.00015	0.33432	0.10
laboratory	summer, nutrients added, no acidified	2	8		37.83	21.5	7.88	1233.82	402.07	12.6270	5.2320	671	5032	4073	27267	3420	1590000	677.8	0.00019	0.00019	0.30701	0.19
laboratory	summer, nutrients added, no acidified	2	9		37.83	21.3	7.86	974.72	246.76	15.4320	3.8940	671	3420	3237	33285	3059	1720000	302.2	0.00025	0.00022	0.32163	0.12
laboratory	Summer, no nutrients added, acidified	1	0	2541	37.83	22.0	8.05	192.78	35.03	0.5600	1.8700	112	644	2055	16066	2140	798990	606.1	0.00012	0.00004	0.06534	2.89	8	12.92	427.30	428.72	1981.54	229.19	2223.64	3.52	5.37
laboratory	Summer, no nutrients added, acidified	1	1	2521	37.83	22.0	7.89	377.70	212.06	0.4000	1.0400	200	1639	4075	4645	1099	521000	292.0	0.00010	0.00002	0.07975	2.81	8	19.87	657.34	659.53	2108.92	168.75	2297.54	2.59	3.95
laboratory	Summer, no nutrients added, acidified	1	2		37.83	22.0	7.80	633.57	100.25	0.2000	1.5000	167	1014	2645	4177		372000	239.7	0.00010	0.00002	0.04937	1.79
laboratory	Summer, no nutrients added, acidified	1	3		37.83	22.0	7.71	706.67	273.75	0.1000	1.1400	384	2398	2182	2612	1973	317000	210.9	0.00012	0.00002	0.10851	1.52
laboratory	Summer, no nutrients added, acidified	1	4		37.83	22.0	7.73	560.46	289.18	0.1200	1.1600	348	1568	1110	2700	888	379000	159.5	0.00018	0.00004	0.14653	2.19
laboratory	Summer, no nutrients added, acidified	1	5		37.83	22.0	7.58	633.57	292.42	0.1000	0.8170	573	1236	628	2752	258	484000	198.2	0.00014	0.00003	0.09178	0.04
laboratory	Summer, no nutrients added, acidified	1	6	2536	37.83	22.2	7.63	645.75	414.26	0.1600	0.9800	540	2171	603	3508	307	538000	169.1	0.00018	0.00006	0.09708	3.19	8	39.09	1299.87	1304.19	2289.01	101.41	2429.50	1.56	2.38
laboratory	Summer, no nutrients added, acidified	1	7		37.83	22.0	7.62	584.83	323.88	0.0200	0.8800	666	2042	608	4237	573	683000	205.9	0.00017	0.00004	0.08968	0.07
laboratory	Summer, no nutrients added, acidified	1	8		37.83	21.6	7.60	584.83	239.05	0.1200	1.0800	425	1373	482	4763	825	674000	185.9	0.00015	0.00003	0.06832	0.09
laboratory	Summer, no nutrients added, acidified	1	9		37.83	21.7	7.53	511.73	269.90	0.3550	1.4050	392	926	428	4451	956	645000	121.7	0.00015	0.00004	0.10160	0.12
laboratory	Summer, no nutrients added, acidified	2	0	2541	37.83	22.0	8.06	279.54	36.55	0.2800	2.1200	107	1009	2458	13087	1661	818800	585.0	0.00012	0.00005	0.06362	3.11	8	12.56	415.44	416.82	1971.39	233.32	2217.27	3.58	5.47
laboratory	Summer, no nutrients added, acidified	2	1	2525	37.83	22.0	7.82	365.52	246.76	0.2800	1.4400	121	795	4445	4898	1041	572000	315.3	0.00009	0.00002	0.06611	2.72	8	23.97	792.91	795.54	2165.16	147.46	2336.59	2.27	3.46
laboratory	Summer, no nutrients added, acidified	2	2		37.83	22.0	7.73	621.38	131.09	0.0600	1.6000	208	1568	5133	2974	3338	422000	251.1	0.00009	0.00002	0.04658	1.89
laboratory	Summer, no nutrients added, acidified	2	3		37.83	21.8	7.67	609.20	366.29	0.1400	1.3600	258	1839	2354	2250	507	350000	177.2	0.00010	0.00002	0.09786	1.46
laboratory	Summer, no nutrients added, acidified	2	4		37.83	21.7	7.70	597.02	487.36	0.0800	1.4000	334	2075	1225	2445	230	395000	159.5	0.00018	0.00004	0.11735	2.42
laboratory	Summer, no nutrients added, acidified	2	5		37.83	22.1	7.55	816.33	572.65	0.0000	0.9000	343	1727	869	2636	291	496000	140.5	0.00013	0.00003	0.07350	0.05
laboratory	Summer, no nutrients added, acidified	2	6	2545	37.83	22.1	7.61	901.62	682.30	0.0400	1.1400	619	2609	691	3072	293	555000	121.0	0.00018	0.00004	0.13243	2.79	8	41.36	1371.71	1376.27	2308.18	97.29	2446.83	1.50	2.28
laboratory	Summer, no nutrients added, acidified	2	7		37.83	22.1	7.61	877.25	215.92	0.0200	1.3200	688	2020	822	3571	724	773000	157.8	0.00017	0.00004	0.08941	0.09
laboratory	Summer, no nutrients added, acidified	2	8		37.83	21.6	7.57	731.04	365.52	0.0200	1.3200	433	1844	625	3996	422	911000	228.6	0.00016	0.00002	0.09285	0.08
laboratory	Summer, no nutrients added, acidified	2	9		37.83	21.5	7.50	609.20	331.59	0.2950	1.2800	463	1759	608	4569	354	880000	144.0	0.00017	0.00004	0.12747	0.15
laboratory	Summer, no nutrients added, no acidified	1	0	2541	37.83	22.0	8.06	308.46	36.55	0.5600	2.2700	132	803	2719	14884	1611	818440	459.1	0.00008	0.00003	0.05754	2.70	8	12.56	415.44	416.82	1971.39	233.32	2217.27	3.58	5.47
laboratory	Summer, no nutrients added, no acidified	1	1	2529	37.83	22.0	8.02	365.52	212.06	0.1800	0.9200	90	861	3985	4996	1000	563000	378.2	0.00009	0.00001	0.05195	2.42	8	13.98	462.43	463.97	2001.32	216.02	2231.33	3.32	5.06
laboratory	Summer, no nutrients added, no acidified	1	2		37.83	22.0	8.01	0.00	96.39	0.0600	0.5200	115	1296	4966	2658	2634	373000	242.8	0.00009	0.00001	0.04201	1.56
laboratory	Summer, no nutrients added, no acidified	1	3		37.83	21.0	7.96	523.91	289.18	0.1600	0.9800	217	1447	2576	2217	600	328000	183.3	0.00014	0.00001	0.09402	1.35
laboratory	Summer, no nutrients added, no acidified	1	4		37.83	21.0	7.97	462.99	231.34	0.0600	0.7200	195	1228	1217	1998	219	371000	146.8	0.00018	0.00003	0.11892	2.22
laboratory	Summer, no nutrients added, no acidified	1	5		37.83	21.1	7.94	462.99	254.48	0.1600	0.7200	217	1203	704	2395	178	420000	104.6	0.00015	0.00002	0.07448	0.00
laboratory	Summer, no nutrients added, no acidified	1	6	2533	37.83	21.1	7.91	511.73	323.88	0.0800	0.7200	356	1477	551	2434	263	456000	132.0	0.00018	0.00004	0.07939	2.32	8	19.39	626.02	628.12	2114.60	171.33	2305.32	2.62	4.01
laboratory	Summer, no nutrients added, no acidified	1	7		37.83	20.8	7.88	536.10	268.05	0.1200	1.0000	384	1491	463	2513	266	537000	101.3	0.00018	0.00002	0.06668	0.02
laboratory	Summer, no nutrients added, no acidified	1	8		37.83	20.8	7.87	621.38	200.50	0.0400	0.7800	543	1384	381	2160	249	545000	114.2	0.00016	0.00001	0.05706	0.04
laboratory	Summer, no nutrients added, no acidified	1	9		37.83	20.6	7.84	645.75	308.46	0.2750	1.1900	499	1184	466	1620	266	572000	81.9	0.00019	0.00003	0.07766	0.04
laboratory	Summer, no nutrients added, no acidified	2	0	2541	37.83	22.0	8.06	371.11	39.60	0.3600	1.8600	151	765	2647	12914	1535	793790	637.6	0.00011	0.00004	0.06092	3.03	8	12.56	415.44	416.82	1971.39	233.32	2217.27	3.58	5.47
laboratory	Summer, no nutrients added, no acidified	2	1	2524	37.83	22.0	8.01	365.52	242.91	0.3200	1.6200	189	1009	4574	6471	1020	485000	283.9	0.00009	0.00001	0.05598	2.35	8	14.35	474.49	476.07	2006.77	211.68	2232.80	3.25	4.96
laboratory	Summer, no nutrients added, no acidified	2	2		37.83	22.0	8.01	536.10	111.82	0.0400	0.8200	121	1233	4706	2415	1118	399000	226.6	0.00009	0.00001	0.04189	1.65
laboratory	Summer, no nutrients added, no acidified	2	3		37.83	21.1	7.96	523.91	320.02	0.1200	1.2600	118	1581	2256	2149	477	315000	192.0	0.00013	0.00001	0.10762	1.33
laboratory	Summer, no nutrients added, no acidified	2	4		37.83	21.0	7.92	475.18	389.89	0.0400	1.1800	181	1288	1088	2182	266	389000	127.5	0.00018	0.00003	0.11307	2.08
laboratory	Summer, no nutrients added, no acidified	2	5		37.83	21.7	7.89	487.36	320.02	0.0400	1.1000	299	1231	677	2280	200	446000	115.1	0.00015	0.00002	0.07082	0.00
laboratory	Summer, no nutrients added, no acidified	2	6	2539	37.83	21.6	7.86	523.91	269.90	0.0800	0.6800	452	1417	537	2557	291	495000	107.7	0.00019	0.00004	0.07271	2.33	8	21.92	717.23	719.63	2152.68	158.38	2332.98	2.43	3.71
laboratory	Summer, no nutrients added, no acidified	2	7		37.83	21.7	7.86	597.02	353.34	0.0400	0.8800	425	1431	521	2650	244	541000	88.4	0.00018	0.00003	0.06314	0.02
laboratory	Summer, no nutrients added, no acidified	2	8		37.83	21.1	7.85	682.30	343.16	0.0400	0.7600	688	1729	469	1932	274	532000	97.7	0.00015	0.00001	0.04836	0.05
laboratory	Summer, no nutrients added, no acidified	2	9		37.83	20.9	7.84	718.86	414.26	0.3150	0.8000	641	1357	496	1661	375	533000	64.0	0.00019	0.00003	0.07603	0.06