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García-Gómez, Candela; Gordillo, Francisco J L; Palma, Armando; Lorenzo, M Rosario; Segovia, María (2014): Seawater carbonate chemistry and the physiological performance of the chlorophyte Dunaliella tertiolecta [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.933580

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Abstract:
The effects of increased CO2 and irradiance on the physiological performance of the chlorophyte Dunaliella tertiolecta were studied at different PAR and UVR (UVA+UVB) irradiances, simulating the solar radiation at different depths, under present (390 ppmv, LC) and predicted CO2 levels for the year 2100 (1000 ppmv, HC). Elevated CO2 resulted in higher optimum and effective quantum yields (Fv/Fm and Phi PSII, respectively), electron transport rates (ETR) and specific growth rates (µ). Cell stress was alleviated in HC respect to LC as evidenced by a decrease in reactive oxygen species (ROS) accumulation. DNA damage showed a 42-fold increase in cyclobutane-pyrimidine dimers (CPDs) formation under the highest irradiance (1100 µmol quanta/m**2/s) in LC with respect to the lowest irradiance (200 µmol quanta/m**2/s). Photolyase (CII-PCD-PL) gene expression was upregulated under HC resulting in a drastic decrease in CPDs accumulation to only 25% with respect to LC. Proliferating cell nuclear antigen (PCNA) accumulation was always higher in HC and the accumulation pattern indicated its involvement in repair or growth depending on the irradiance dose. The repressor of silencing (ROS1) was only marginally involved in the response, suggesting that photoreactivation was the most relevant mechanism to overcome UVR damage. Our results demonstrate that future scenarios of global change result in alleviation of irradiance stress by CO2–induced photoprotection in D. tertiolecta.
Keyword(s):
Bottles or small containers/Aquaria (<20 L); Chlorophyta; Dunaliella tertiolecta; Gene expression (incl. proteomics); Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Macroalgae; Not applicable; Other studied parameter or process; Pelagos; Plantae; Primary production/Photosynthesis; Single species
Supplement to:
García-Gómez, Candela; Gordillo, Francisco J L; Palma, Armando; Lorenzo, M Rosario; Segovia, María (2014): Elevated CO2 alleviates high PAR and UV stress in the unicellular chlorophyte Dunaliella tertiolecta. Photochemical & Photobiological Sciences, 13(9), 1347, https://doi.org/10.1039/c4pp00044g
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-07-06.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
TypeTypeGarcía-Gómez, Candelastudy
SpeciesSpeciesGarcía-Gómez, Candela
Registration number of speciesReg spec noGarcía-Gómez, Candela
Uniform resource locator/link to referenceURL refGarcía-Gómez, CandelaWoRMS Aphia ID
Time point, descriptiveTime pointGarcía-Gómez, Candela
Experiment durationExp durationdaysGarcía-Gómez, Candela
ReplicateReplGarcía-Gómez, Candela
PositionPositionGarcía-Gómez, Candela
TreatmentTreatGarcía-Gómez, Candela
10 IrradianceEµmol/m2/sGarcía-Gómez, Candela
11 Growth rateµ1/dayGarcía-Gómez, Candela
12 Maximum quantum yield of photosystem IIFv/FmGarcía-Gómez, Candela
13 Maximum quantum yield of photosystem II, standard deviationFv/Fm std dev±García-Gómez, Candela
14 Reactive oxygen speciesROSGarcía-Gómez, Candela
15 Cyclobutane pyrimidine dimers accumulationCPD accGarcía-Gómez, Candela
16 Proliferating cell nuclear antigen accumulationPCNA accGarcía-Gómez, Candela
17 Repressor of silencing accumulationROS1 accGarcía-Gómez, Candela
18 Gene expressionGene expressionGarcía-Gómez, CandelaClass II-CPD-photolyase
19 SalinitySalGarcía-Gómez, Candela
20 Temperature, waterTemp°CGarcía-Gómez, Candela
21 Alkalinity, totalATµmol/lGarcía-Gómez, Candela
22 Alkalinity, totalATµmol/kgGarcía-Gómez, Candela
23 pHpHGarcía-Gómez, Candelatotal scale
24 pH, standard deviationpH std dev±García-Gómez, Candelatotal scale
25 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmGarcía-Gómez, Candela
26 Partial pressure of carbon dioxide, standard deviationpCO2 std dev±García-Gómez, Candela
27 Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
28 Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
29 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
30 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
31 Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32 Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33 Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34 Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35 Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
696 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Type
(study)

Species

Reg spec no

URL ref
(WoRMS Aphia ID)

Time point

Exp duration [days]

Repl

Position

Treat
10 
E [µmol/m2/s]
11 
µ [1/day]
12 
Fv/Fm
13 
Fv/Fm std dev [±]
14 
ROS
15 
CPD acc
16 
PCNA acc
17 
ROS1 acc
18 
Gene expression
(Class II-CPD-photolyase)
19 
Sal
20 
Temp [°C]
21 
AT [µmol/l]
22 
AT [µmol/kg]
23 
pH
(total scale)
24 
pH std dev [±]
(total scale)
25 
pCO2water_SST_wet [µatm]
26 
pCO2 std dev [±]
27 
CSC flag
28 
CO2 [µmol/kg]
29 
fCO2water_SST_wet [µatm]
30 
pCO2water_SST_wet [µatm]
31 
[HCO3]- [µmol/kg]
32 
[CO3]2- [µmol/kg]
33 
DIC [µmol/kg]
34 
Omega Arg
35 
Omega Cal
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgInitial0ALow CO25000.420.6550.0062442.5587471.2178.6655.901.0034.51622002145.588.110.003257811.41313.14314.261721.11169.221901.742.604.05
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgInitial0BLow CO25000.450.5980.0182200.2284603.6366.7237.471.0034.51622002145.588.110.003257811.41313.14314.261721.11169.221901.742.604.05
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgInitial0CHigh CO25000.670.6560.0031677.1556501.6639.5621.221.0034.51622002145.587.700.0095617833.38915.59918.871957.8374.892066.091.151.79
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgInitial0DHigh CO25000.630.6570.0102191.8346446.5584.2337.111.0034.51622002145.587.700.0095617833.38915.59918.871957.8374.892066.091.151.79
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6A1Low CO211000.100.0490.0324672.464488282.2628.1746.55592.0434.51622002145.588.020.13432146814.58400.07401.501787.33142.841944.752.203.42
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6B1Low CO211000.050.0060.0065636.169238280.2626.8648.41334.9634.51622002145.588.020.13432146814.58400.07401.501787.33142.841944.752.203.42
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6C1High CO211000.180.0950.0543255.73246180.7268.4146.005906.6434.51622002145.587.830.0168713824.01658.61660.971899.7898.032021.821.512.35
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6D1High CO211000.290.2360.0143760.63448160.5861.1229.535631.7234.51622002145.587.830.0168713824.01658.61660.971899.7898.032021.821.512.35
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6A2Low CO28000.310.1670.0065048.04322435.7037.6530.1479.3434.51622002145.588.330.00175786.08166.87167.471522.17248.371776.623.825.94
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6B2Low CO28000.180.1730.0114494.08586434.7281.3660.44468.8734.51622002145.588.330.00175786.08166.87167.471522.17248.371776.623.825.94
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6C2High CO28000.390.3010.0254780.49159639.7283.3635.0927.6334.51622002145.587.920.0455161819.01521.57523.441850.93117.501987.441.812.81
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6D2High CO28000.490.3820.0012826.87186816.8196.4423.89128.2034.51622002145.587.920.0455161819.01521.57523.441850.93117.501987.441.812.81
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6A3Low CO24000.410.2230.0054077.42758517.3126.9623.2625.6734.51622002145.588.390.04146685.08139.33139.831459.22273.371737.684.216.54
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6B3Low CO24000.370.2500.0163793.56525854.3740.8942.5156.4634.51622002145.588.390.04146685.08139.33139.831459.22273.371737.684.216.54
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6C3High CO24000.510.3990.0071449.1913144.2374.4926.096.8734.51622002145.588.120.0531844811.10304.60305.691713.20172.361896.672.654.13
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6D3High CO24000.450.3980.0011773.81731220.8069.0324.0343.3934.51622002145.588.120.0531844811.10304.60305.691713.20172.361896.672.654.13
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6A4Low CO22000.400.4270.0053760.6344812.3864.6635.1414.9534.51622002145.588.300.01189486.65182.35183.001552.32236.381795.353.645.66
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6B4Low CO22000.470.5140.0062352.52665010.9465.7854.4715.0634.51622002145.588.300.01189486.65182.35183.001552.32236.381795.353.645.66
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6C4High CO22000.470.5910.0111737.4809426.0056.8729.6274.2434.51622002145.588.100.0633759811.73321.88323.031728.90166.111906.752.563.98
laboratoryDunaliella tertiolecta (macroalga)178590marinespecies.orgFinal6D4High CO22000.520.5810.0061201.7709772.7750.2016.87215.5234.51622002145.588.100.0633759811.73321.88323.031728.90166.111906.752.563.98