Citation:

PANGAEA.933100

Name: Seawater carbonate chemistry and ion-transporting capacity and aerobic respiration of larval white seabass (Atractoscion nobilis)
Published: 2021-06-28
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Atractoscion nobilis; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Nekton; North Atlantic; Other studied parameter or process; Pelagos; Respiration; Single species; Temperate

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Abstract:

Ocean acidification (OA) has been proposed to increase the energetic demand for acid-base regulation at the expense of larval fish growth. Here, white seabass (Atractoscion nobilis) eggs and larvae were reared at control (542 +/- 28 μatm) and elevated pCO2 (1,831 +/- 105 μatm) until five days post-fertilization (dpf). Skin ionocytes were identified by immunodetection of the Na+/K+-ATPase (NKA) enzyme. Larvae exposed to elevated pCO2 possessed significantly higher skin ionocyte number and density compared to control larvae. However, when ionocyte size was accounted for, the relative ionocyte area (a proxy for total ionoregulatory capacity) was unchanged. Similarly, there were no differences in relative NKA abundance, resting O2 consumption rate, and total length between control and treatment larvae at 5 dpf, nor in the rate at which relative ionocyte area and total length changed between 2–5 dpf. Altogether, our results suggest that OA conditions projected for the next century do not significantly affect the ionoregulatory capacity or energy consumption of larval white seabass. Finally, a retroactive analysis of the water in the recirculating aquarium system that housed the broodstock revealed the parents had been exposed to average pCO2 of 1,200 μatm for at least 3.5 years prior to this experiment. Future studies should investigate whether larval white seabass are naturally resilient to OA, or if this resilience is the result of parental chronic acclimation to OA, and/or from natural selection during spawning and fertilization in elevated pCO2.

Keyword(s):

Animalia; Atractoscion nobilis; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Nekton; North Atlantic; Other studied parameter or process; Pelagos; Respiration; Single species; Temperate

Supplement to:

Kwan, Garfield Tsz; Shen, Sara G; Drawbridge, Mark; Checkley, David M; Tresguerres, Martin (2021): Ion-transporting capacity and aerobic respiration of larval white seabass (Atractoscion nobilis) may be resilient to ocean acidification conditions. Science of the Total Environment, 791, 148285, https://doi.org/10.1016/j.scitotenv.2021.148285

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-06-24.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Shen, Sara G		study
2	Species	Species		Shen, Sara G
3	Registration number of species	Reg spec no		Shen, Sara G
4	Uniform resource locator/link to reference	URL ref		Shen, Sara G		WoRMS Aphia ID
5	Treatment	Treat		Shen, Sara G
6	pH	pH		Shen, Sara G		Experimental vessel
7	pH, standard error	pH std e	±	Shen, Sara G		Experimental vessel
8	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Shen, Sara G		Experimental vessel
9	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Shen, Sara G		Experimental vessel
10	Length, total	TL	mm	Shen, Sara G		Fresh
11	Length, total, standard error	TL std e	±	Shen, Sara G		Fresh
12	Growth rate	µ	mm/day	Shen, Sara G
13	Growth rate, standard error	µ std e	±	Shen, Sara G
14	Ionocyte number	Ionocyte No	#	Shen, Sara G		per mm2
15	Ionocyte number, standard error	Ionocyte No std e	±	Shen, Sara G		per mm2
16	Surface area	SA	mm²	Shen, Sara G		Larval
17	Surface area, standard error	SA std e	±	Shen, Sara G		Larval
18	Ionocyte density	Ionocyte dens	#/µm²	Shen, Sara G
19	Ionocyte density, standard error	Ionocyte dens std e	±	Shen, Sara G
20	Ionocyte size	Ionocyte size	µm²	Shen, Sara G
21	Ionocyte size, standard error	Ionocyte size std e	±	Shen, Sara G
22	Relative ionocyte area	RIA	%	Shen, Sara G
23	Relative ionocyte area, standard error	RIA std e	±	Shen, Sara G
24	Relative Na+/K+-ATPase-rich abundance	Rel NKA abund		Shen, Sara G
25	Relative Na+/K+-ATPase-rich abundance, standard error	Rel NKA abund std e	±	Shen, Sara G
26	Relative ionocyte area per day	RIA/day	%/day	Shen, Sara G		over 2-5 days post-fertilization
27	Relative ionocyte area per day, standard error	RIA/day std e	±	Shen, Sara G		over 2-5 days post-fertilization
28	Oxygen consumption per individual	O2 con/ind	nl/#/h	Shen, Sara G		Resting
29	Oxygen consumption, standard error	O2 con std e	±	Shen, Sara G		Resting
30	Experiment	Exp		Shen, Sara G
31	Vessel	Vessel		Shen, Sara G		Vessel
32	Time in days	Time	days	Shen, Sara G		Days post-fertilization
33	Length, total	TL	mm	Shen, Sara G		Average
34	Length, total	TL	mm	Shen, Sara G
35	Relative ionocyte area	RIA	%	Shen, Sara G
36	Ionocyte number	Ionocyte No	#	Shen, Sara G
37	Ionocyte size	Ionocyte size	µm²	Shen, Sara G
38	Surface area	SA	mm²	Shen, Sara G
39	Ionocyte density	Ionocyte dens	#/µm²	Shen, Sara G
40	Relative Na+/K+-ATPase-rich abundance	Rel NKA abund		Shen, Sara G
41	Oxygen consumption per individual	O2 con/ind	nl/#/h	Shen, Sara G		Resting
42	Salinity	Sal		Shen, Sara G
43	Temperature, water	Temp	°C	Shen, Sara G
44	Alkalinity, total	AT	µmol/kg	Shen, Sara G
45	Carbon, inorganic, dissolved	DIC	µmol/kg	Shen, Sara G
46	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
48	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1972 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 pH (Experimental vessel)	7 pH std e [±] (Experimental vessel)	8 pCO2water_SST_wet [µatm] (Experimental vessel)	9 pCO2water_SST_wet std e [±] (Experimental vessel)	10 TL [mm] (Fresh)	11 TL std e [±] (Fresh)	12 µ [mm/day]	13 µ std e [±]	14 Ionocyte No [#] (per mm2)	15 Ionocyte No std e [±] (per mm2)	16 SA [mm²] (Larval)	17 SA std e [±] (Larval)	18 Ionocyte dens [#/µm²]	19 Ionocyte dens std e [±]	20 Ionocyte size [µm²]	21 Ionocyte size std e [±]	22 RIA [%]	23 RIA std e [±]	24 Rel NKA abund	25 Rel NKA abund std e [±]	26 RIA/day [%/day] (over 2-5 days post-fertilization)	27 RIA/day std e [±] (over 2-5 days post-fertilization)	28 O2 con/ind [nl/#/h] (Resting)	29 O2 con std e [±] (Resting)	30 Exp	31 Vessel (Vessel)	32 Time [days] (Days post-fertilization)	33 TL [mm] (Average)	34 TL [mm]	35 RIA [%]	36 Ionocyte No [#]	37 Ionocyte size [µm²]	38 SA [mm²]	39 Ionocyte dens [#/µm²]	40 Rel NKA abund	41 O2 con/ind [nl/#/h] (Resting)	42 Sal	43 Temp [°C]	44 AT [µmol/kg]	45 DIC [µmol/kg]	46 CSC flag	47 pH (total scale)	48 CO2 [µmol/kg]	49 fCO2water_SST_wet [µatm]	50 pCO2water_SST_wet [µatm]	51 [HCO3]- [µmol/kg]	52 [CO3]2- [µmol/kg]	53 Omega Arg	54 Omega Cal
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control	7.92	0.02	560	32	3.57	0.04	0.129	0.083	564.6	52.43	1.833	0.04	309.6	11.62	205.94	28.34	6.14	0.74	1.00	0.14	-1.46	0.51	180	30
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA	7.42	0.03	1971	55	3.59	0.03	0.060	0.019	688.2	18.59	1.833	0.17	379.7	12.51	152.09	7.44	5.73	0.27	0.73	0.15	-1.22	0.30	190	30
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1	1	5	3.74									33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1	2	5	3.58									33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1	3	5	3.61									33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1	1	5	3.58									33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1	2	5	3.63									33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1	3	5	3.67									33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2	1	5	3.49									33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2	2	5	3.56									33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2	3	5	3.43									33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2	1	5	3.64									33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2	2	5	3.54									33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2	3	5	3.50									33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		2		2.9	8.6957							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		2		2.7	10.7508							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		2		2.9	8.6773							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		2		2.9	7.3310							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		2		3.0	8.3506							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		2		2.9	8.0661							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		3		2.9	5.0334							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		3		3.1	9.9453							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		3		2.9	5.7583							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		3		3.1	9.3398							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		3		2.9	9.7218							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		3		2.8	6.6251							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		4		3.1	9.0471							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		4		3.1	4.5578							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		4		2.8	8.6746							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		4		3.1	5.0003							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		4		3.1	5.9174							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		4		3.1	6.1165							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		5		3.1	3.4099							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		5		3.3	3.7067							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		5		3.2	5.8826							33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		5		3.2	4.7101							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		5		3.1	4.6186							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		5		3.1	5.1401							33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1		5			7.4500	680	274	2.5	272			33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1		5			7.1400	654	251	2.3	283			33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1		5			10.7700	829	364	2.6	315			33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1		5			6.4500	733	167	1.9	392			33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1		5			7.1500	609	199	1.7	359			33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1		5			4.7700	690	145	2.1	329			33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2		5			5.5900	472	166	1.4	336			33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2		5			4.9500	376	171	1.3	296			33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2		5			6.3800	379	253	1.5	261			33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2		5			5.7400	748	130	1.7	440			33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2		5			5.9800	746	144	1.8	416			33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2		5			6.9800	743	169	1.8	413			33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		5			3.4100	568	102	1.7	329			33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		5			3.7100	443	117	1.4	323			33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		5			5.8800	680	156	1.8	371			33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		5			4.7100	635	141	1.9	341			33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		5			4.6200	637	131	1.8	359			33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		5			5.1400	653	142	1.8	368			33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1		5								1.280		33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1		5								0.384		33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1		5								1.296		33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1		5								0.592		33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1		5								0.576		33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1		5								1.504		33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2		5								1.088		33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2		5								1.104		33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2		5								1.504		33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2		5								1.168		33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2		5								0.464		33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2		5								0.912		33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		5								0.832		33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		5								0.512		33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		5								0.448		33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		5								0.192		33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1		5									240	33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1		5									130	33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									1		5									200	33.64	18.00	2267.37	2096.83	15	7.92	19.47	563.45	565.41	1947.74	129.63	2.02	3.13
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1		5									160	33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1		5									190	33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									1		5									150	33.63	18.03	2262.07	2272.37	15	7.39	72.63	2104.59	2111.95	2157.17	42.53	0.66	1.03
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2		5									110	33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2		5									90	33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									2		5									100	33.62	18.00	2266.90	2078.30	15	7.96	17.50	507.11	508.88	1920.16	140.62	2.19	3.39
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2		5									150	33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2		5									120	33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									2		5									110	33.61	18.03	2265.37	2254.37	15	7.46	61.53	1783.44	1789.67	2143.25	49.57	0.77	1.20
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		5									310	33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	Control																									3		5									270	33.63	18.05	2252.80	2096.80	15	7.88	21.65	627.36	629.54	1954.19	120.98	1.88	2.92
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		5									340	33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06
laboratory	Atractoscion nobilis (fish)	278535	marinespecies.org	OA																									3		5									260	33.63	18.05	2253.55	2259.25	15	7.40	69.90	2025.97	2033.05	2145.59	43.80	0.68	1.06