Citation:

PANGAEA.932922

Name: Seawater carbonate chemistry and acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata)
Published: 2021-06-23
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Haliotis tuberculata; Laboratory experiment; Mollusca; North Atlantic; Single species; Temperate

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Abstract:

Ocean acidification (OA) and the associated changes in seawater carbonate chemistry pose a threat to calcifying organisms. This is particularly serious for shelled molluscs, in which shell growth and microstructure has been shown to be highly sensitive to OA. To improve our understanding of the responses of abalone to OA, this study investigated the effects of CO2-induced ocean acidification on extra-cellular acid–base parameters in the European abalone Haliotis tuberculata. Three-year-old adult abalone were exposed for 15 days to three different pH levels (7.9, 7.7, 7.4) representing current and predicted near-future conditions. Hæmolymph pH and total alkalinity were measured at different time points during exposure and used to calculate the carbonate parameters of the extracellular fluid. Total protein content was also measured to determine whether seawater acidification influences the composition and buffer capacity of hæmolymph. Extracellular pH was maintained at seawater pH 7.7 indicating that abalones are able to buffer moderate acidification (−0.2 pH units). This was not due to an accumulation of HCO3− ions but rather to a high hæmolymph protein concentration. By contrast, hæmolymph pH was significantly decreased after 5 days of exposure to pH 7.4, indicating that abalone do not compensate for higher decreases in seawater pH. Total alkalinity and dissolved inorganic carbon were also significantly decreased after 15 days of low pH exposure. It is concluded that changes in the acid–base balance of the hæmolymph might be involved in deleterious effects recorded in adult H. tuberculata facing severe OA stress. This would impact both the ecology and aquaculture of this commercially important species.

Keyword(s):

Acid-base regulation; Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Haliotis tuberculata; Laboratory experiment; Mollusca; North Atlantic; Single species; Temperate

Supplement to:

Auzoux-Bordenave, Stephanie; Chevret, Sandra; Badou, Aïcha; Martin, Sophie; Di Giglio, Sarah; Dubois, Philippe (2021): Acid–base balance in the hæmolymph of European abalone (Haliotis tuberculata) exposed to CO2-induced ocean acidification. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 259, 110996, https://doi.org/10.1016/j.cbpa.2021.110996

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 48.613800 * Longitude: -4.600800

Event(s):

France_Haliotis_abalone_farm * Latitude: 48.613800 * Longitude: -4.600800 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-06-22.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Auzoux-Bordenave, Stephanie		study
2	Species	Species		Auzoux-Bordenave, Stephanie
3	Registration number of species	Reg spec no		Auzoux-Bordenave, Stephanie
4	Uniform resource locator/link to reference	URL ref		Auzoux-Bordenave, Stephanie		WoRMS Aphia ID
5	Experiment	Exp		Auzoux-Bordenave, Stephanie
6	Experiment duration	Exp duration	days	Auzoux-Bordenave, Stephanie
7	Treatment	Treat		Auzoux-Bordenave, Stephanie
8	Salinity	Sal		Auzoux-Bordenave, Stephanie
9	Salinity, standard deviation	Sal std dev	±	Auzoux-Bordenave, Stephanie
10	pH	pH		Auzoux-Bordenave, Stephanie	Potentiometric	total scale
11	pH, standard deviation	pH std dev	±	Auzoux-Bordenave, Stephanie	Potentiometric	total scale
12	Temperature, water	Temp	°C	Auzoux-Bordenave, Stephanie
13	Temperature, water, standard deviation	Temp std dev	±	Auzoux-Bordenave, Stephanie
14	Alkalinity, total	AT	µmol/kg	Auzoux-Bordenave, Stephanie	Potentiometric titration
15	Alkalinity, total, standard deviation	AT std dev	±	Auzoux-Bordenave, Stephanie	Potentiometric titration
16	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
17	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
18	Carbon, inorganic, dissolved	DIC	µmol/kg	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
19	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
20	Bicarbonate ion	[HCO3]-	µmol/kg	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
21	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
22	Carbonate ion	[CO3]2-	µmol/kg	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
23	Carbonate ion, standard deviation	[CO3]2- std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
24	Aragonite saturation state	Omega Arg		Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
25	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
26	Calcite saturation state	Omega Cal		Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
27	Calcite saturation state, standard deviation	Omega Cal std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
28	Haemolymph, pH	pH (ha)		Auzoux-Bordenave, Stephanie	Potentiometric	total scale
29	Haemolymph, pH, standard deviation	pH (ha) std dev	±	Auzoux-Bordenave, Stephanie	Potentiometric	total scale
30	Haemolymph, temperature	Temp (ha) std dev	°C	Auzoux-Bordenave, Stephanie
31	Haemolymph, temperature, standard deviation	Temp (ha) std dev	±	Auzoux-Bordenave, Stephanie
32	Haemolymph, alkalinity, total	AT (ha)	µmol/kg	Auzoux-Bordenave, Stephanie	Potentiometric titration
33	Haemolymph, alkalinity, total, standard deviation	AT (ha) std dev	±	Auzoux-Bordenave, Stephanie	Potentiometric titration
34	Haemolymph, partial pressure of carbon dioxide	pCO2 (ha)	µatm	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
35	Haemolymph, partial pressure of carbon dioxide, standard deviation	pCO2 (ha) std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
36	Haemolymph, total dissolved inorganic carbon	DIC (ha)	µmol/kg	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
37	Haemolymph, total dissolved inorganic carbon, standard deviation	DIC (ha) std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
38	Haemolymph, bicarbonate ion	[HCO3]- (ha)	µmol/kg	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
39	Haemolymph, bicarbonate ion, standard deviation	[HCO3]- (ha) std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
40	Haemolymph, carbonate ion	[CO3]2- (ha)	µmol/kg	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
41	Haemolymph, carbonate ion, standard deviation	[CO3]2- (ha) std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
42	Haemolymph, aragonite saturation state	Omega Arg (ha)		Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
43	Haemolymph, aragonite saturation state, standard deviation	Omega Arg (ha) std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
44	Haemolymph, calcite saturation state	Omega Cal (ha)		Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
45	Haemolymph, calcite saturation state, standard deviation	Omega Cal (ha) std dev	±	Auzoux-Bordenave, Stephanie	Calculated using CO2SYS
46	Proteins, total	TPRT	g/l	Auzoux-Bordenave, Stephanie
47	Proteins, total, standard deviation	TPRT std dev	±	Auzoux-Bordenave, Stephanie
48	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
51	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
53	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
55	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
57	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
59	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
61	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
63	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

637 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp	6 Exp duration [days]	7 Treat	8 Sal	9 Sal std dev [±]	10 pH (total scale, Potentiometric)	11 pH std dev [±] (total scale, Potentiometric)	12 Temp [°C]	13 Temp std dev [±]	14 AT [µmol/kg] (Potentiometric titration)	15 AT std dev [±] (Potentiometric titration)	16 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	17 pCO2 std dev [±] (Calculated using CO2SYS)	18 DIC [µmol/kg] (Calculated using CO2SYS)	19 DIC std dev [±] (Calculated using CO2SYS)	20 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	21 [HCO3]- std dev [±] (Calculated using CO2SYS)	22 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	23 [CO3]2- std dev [±] (Calculated using CO2SYS)	24 Omega Arg (Calculated using CO2SYS)	25 Omega Arg std dev [±] (Calculated using CO2SYS)	26 Omega Cal (Calculated using CO2SYS)	27 Omega Cal std dev [±] (Calculated using CO2SYS)	28 pH (ha) (total scale, Potentiometric)	29 pH (ha) std dev [±] (total scale, Potentiometric)	30 Temp (ha) std dev [°C]	31 Temp (ha) std dev [±]	32 AT (ha) [µmol/kg] (Potentiometric titration)	33 AT (ha) std dev [±] (Potentiometric titration)	34 pCO2 (ha) [µatm] (Calculated using CO2SYS)	35 pCO2 (ha) std dev [±] (Calculated using CO2SYS)	36 DIC (ha) [µmol/kg] (Calculated using CO2SYS)	37 DIC (ha) std dev [±] (Calculated using CO2SYS)	38 [HCO3]- (ha) [µmol/kg] (Calculated using CO2SYS)	39 [HCO3]- (ha) std dev [±] (Calculated using CO2SYS)	40 [CO3]2- (ha) [µmol/kg] (Calculated using CO2SYS)	41 [CO3]2- (ha) std dev [±] (Calculated using CO2SYS)	42 Omega Arg (ha) (Calculated using CO2SYS)	43 Omega Arg (ha) std dev [±] (Calculated using CO2SYS)	44 Omega Cal (ha) (Calculated using CO2SYS)	45 Omega Cal (ha) std dev [±] (Calculated using CO2SYS)	46 TPRT [g/l]	47 TPRT std dev [±]	48 CSC flag (Calculated using seacarb afte...)	49 CO2 [µmol/kg] (Calculated using seacarb afte...)	50 CO2 std dev [±] (Calculated using seacarb afte...)	51 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	52 fCO2 std dev [±] (Calculated using seacarb afte...)	53 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	54 pCO2 std dev [±] (Calculated using seacarb afte...)	55 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	56 [HCO3]- std dev [±] (Calculated using seacarb afte...)	57 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	58 [CO3]2- std dev [±] (Calculated using seacarb afte...)	59 DIC [µmol/kg] (Calculated using seacarb afte...)	60 DIC std dev [±] (Calculated using seacarb afte...)	61 Omega Arg (Calculated using seacarb afte...)	62 Omega Arg std dev [±] (Calculated using seacarb afte...)	63 Omega Cal (Calculated using seacarb afte...)	64 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Haliotis tuberculata (mollusk)	140059	marinespecies.org	1		pH=8	34.1	0.1	8.03	0.01	14.5	0.3	3105	21	576	30	2861	18	2639	16	199	15	3.05	0.23	4.75	0.19	7.32	0.16	14.5	0.3	3796	72	4331	1679	3887	155	3664	109	58	16	0.89	0.25	1.39	0.39			8	21.81	0.74	570.55	18.86	572.63	18.93	2632.27	24.21	201.34	7.61	2855.42	21.76	3.09	0.18	4.83	0.29
laboratory	Haliotis tuberculata (mollusk)	140059	marinespecies.org	2	5	pH=7.9	34.6	0.2	7.87	0.01	15.0	0.1	2287	3	633	14	2148	5	2014	6	110	2	1.69	0.03	2.63	0.04	7.32	0.02	15.0	0.1	3189	155	3393	5	3252	150	3077	145	48	4	0.74	0.07	1.15	0.11	10.3	3.3	8	23.74	0.75	632.41	19.99	634.70	20.06	2014.40	9.43	109.94	4.13	2148.08	6.26	1.69	0.10	2.63	0.16
laboratory	Haliotis tuberculata (mollusk)	140059	marinespecies.org	2	5	pH=7.7	34.6	0.2	7.67	0.01	15.1	0.0	2281	6	1056	22	2213	2	2102	2	72	2	1.11	0.02	1.73	0.04	7.20	0.09	15.1	0.0	3130	260	4617	1036	3254	302	3047	266	36	3	0.54	0.04	0.85	0.07	8.3	3.1	8	39.15	1.20	1046.04	32.24	1049.83	32.36	2100.87	8.35	72.62	2.83	2212.64	7.30	1.11	0.07	1.74	0.10
laboratory	Haliotis tuberculata (mollusk)	140059	marinespecies.org	2	5	pH=7.4	34.6	0.2	7.43	0.03	15.1	0.1	2291	1	1913	160	2298	9	2183	7	43	3	0.66	0.05	1.03	0.07	7.09	0.07	15.1	0.1	2910	80	5543	1106	3082	113	2849	83	26	3	0.40	0.05	0.62	0.09	17.1	4.1	8	70.71	5.26	1889.22	140.78	1896.07	141.30	2183.39	7.76	43.43	3.21	2297.54	9.64	0.67	0.06	1.04	0.09
laboratory	Haliotis tuberculata (mollusk)	140059	marinespecies.org	2	10	pH=7.9	34.6	0.2	7.92	0.01	13.9	0.1	2297	4	555	18	2143	8	2003	10	119	3	1.81	0.04	2.83	0.07	7.36	0.07	13.9	0.1	3185	65	3141	569	3239	94	3067	78	50	6	0.76	0.10	1.19	0.15	18.2	4.6	8	21.62	0.68	556.76	17.71	558.81	17.77	2005.02	10.24	117.68	4.39	2144.32	6.97	1.80	0.11	2.81	0.17
laboratory	Haliotis tuberculata (mollusk)	140059	marinespecies.org	2	10	pH=7.7	34.6	0.2	7.66	0.05	13.9	0.1	2294	5	1083	129	2234	16	2123	18	69	7	1.06	0.11	1.65	0.17	7.29	0.03	13.9	0.1	3208	151	3646	129	3290	141	3106	140	44	5	0.67	0.08	1.04	0.12	15.7	3.1	8	41.67	5.21	1073.25	134.14	1077.19	134.64	2123.97	19.29	68.51	7.64	2234.15	16.94	1.05	0.13	1.64	0.20
laboratory	Haliotis tuberculata (mollusk)	140059	marinespecies.org	2	10	pH=7.4	34.6	0.2	7.40	0.02	13.9	0.1	2306	2	2056	119	2328	9	2209	6	39	2	0.60	0.03	0.93	0.04	7.15	0.02	13.9	0.1	2969	148	4801	242	3115	151	2901	143	29	2	0.44	0.04	0.69	0.06	20.4	7.5	8	78.86	4.03	2031.12	104.08	2038.60	104.46	2208.94	5.44	39.15	2.18	2326.96	6.99	0.60	0.04	0.94	0.07
laboratory	Haliotis tuberculata (mollusk)	140059	marinespecies.org	2	15	pH=7.9	34.6	0.2	7.95	0.05	13.4	0.3	2305	4	514	6	2141	3	1996	3	124	1	1.90	0.01	2.96	0.02	7.29	0.06	13.4	0.3	3157	58	3675	560	3246	81	3059	66	41	5	0.63	0.08	0.99	0.12	21.7	5.1	8	20.36	2.69	516.25	67.99	518.16	68.24	1999.06	31.86	123.32	12.96	2142.73	21.73	1.88	0.22	2.95	0.34
laboratory	Haliotis tuberculata (mollusk)	140059	marinespecies.org	2	15	pH=7.7	34.6	0.2	7.75	0.04	13.4	0.2	2303	5	852	76	2212	10	2095	14	84	7	1.27	0.11	1.99	0.17	7.27	0.03	13.4	0.2	3117	68	3776	149	3212	56	3025	60	39	3	0.59	0.05	0.93	0.08	20.3	3.4	8	33.90	3.45	859.57	87.55	862.75	87.88	2100.13	18.52	81.74	7.38	2215.77	14.78	1.25	0.13	1.95	0.20
laboratory	Haliotis tuberculata (mollusk)	140059	marinespecies.org	2	15	pH=7.4	34.6	0.2	7.38	0.07	13.4	0.2	2305	5	2138	390	2333	26	2211	17	38	6	0.58	0.09	0.90	0.14	7.13	0.01	13.4	0.2	2804	57	4644	124	2950	57	2742	55	26	1	0.40	0.01	0.62	0.02	18.4	4.6	8	83.76	14.12	2124.10	357.99	2131.97	359.32	2213.81	15.17	36.76	5.83	2334.33	23.16	0.56	0.09	0.88	0.15