Citation:

PANGAEA.932784

Name: Seawater carbonate chemistry and carbon and nitrogen fixation in the hermatypic coral Galaxea fascicularis
Published: 2021-06-18
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Galaxea fascicularis; Laboratory experiment; North Pacific; Other metabolic rates; Primary production/Photosynthesis; Single species; Tropical

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Abstract:

The supply of metabolites from symbionts to scleractinian corals is crucial to coral health. Members of the Symbiodiniaceae can enhance coral calcification by providing photosynthetically fixed carbon (PFC) and energy, whereas dinitrogen (N2)-fixing bacteria can provide additional nutrients such as diazotrophically-derived nitrogen (DDN) that sustain coral productivity especially when alternative external nitrogen sources are scarce. How these mutualistic associations benefit corals in the future acidifying ocean is not well understood. In this study, we investigated the possible effects of ocean acidification (OA; pHs 7.7 and 7.4 vs. 8.1) on calcification in the hermatypic coral Galaxea fascicularis with respect to PFC and DDN assimilation. Our measurements based on isotopic tracing showed no significant differences in the assimilation of PFC among different pH treatments, but the assimilation of DDN decreased significantly after 28 days of stress at pH 7.4. The decreased DDN assimilation suggests a nitrogenous nutrient deficiency in the coral holotiont, potentially leading to reduced coral calcification and resilience to bleaching and other stressful events. This contrasting impact of OA on carbon and N flux demonstrates the flexibility of G. fascicularis in coping with OA, apparently by sustaining a largely undamaged photosystem at the expense of N2 fixation machinery, which competes with coral calcification for energy from photosynthesis. These findings shed new light on the critically important but more vulnerable N cycling in hospite, and on the trade-off between coral hosts and symbionts in response to future climate change.

Keyword(s):

Animalia; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Galaxea fascicularis; Laboratory experiment; North Pacific; Other metabolic rates; Primary production/Photosynthesis; Single species; Tropical

Supplement to:

Zheng, Xinqing; Wang, Chenying; Sheng, Huaxia; Niu, Gaofeng; Dong, Xu; Yuan, Lingling; Shi, Tuo (2021): Effects of Ocean Acidification on Carbon and Nitrogen Fixation in the Hermatypic Coral Galaxea fascicularis. Frontiers in Marine Science, 8, https://doi.org/10.3389/fmars.2021.644965

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-06-17.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Shi, Tuo		study
2	Species	Species		Shi, Tuo
3	Registration number of species	Reg spec no		Shi, Tuo
4	Uniform resource locator/link to reference	URL ref		Shi, Tuo		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Shi, Tuo
6	Treatment	Treat		Shi, Tuo
7	Effective quantum yield	Y		Shi, Tuo
8	Effective quantum yield, standard deviation	Y std dev	±	Shi, Tuo
9	Maximum quantum yield of photosystem II	Fv/Fm		Shi, Tuo
10	Maximum quantum yield of photosystem II, standard deviation	Fv/Fm std dev	±	Shi, Tuo
11	Calcification rate	Calc rate	%/week	Shi, Tuo
12	Calcification rate, standard deviation	Calc rate std dev	±	Shi, Tuo
13	Photosynthetically fixed carbon assimilation rate, carbon in tracer/carbon in tissue	PFC assim r	nmol/µmol/day	Shi, Tuo
14	Photosynthetically fixed carbon assimilation rate, carbon in tracer/carbon in tissue, standard deviation	PFC assim r std dev	±	Shi, Tuo
15	Diazotrophically-derived nitrogen assimilation rate, nitrogen in tracer/nitrogen in tissue	DDN assim r	nmol/µmol/day	Shi, Tuo
16	Diazotrophically-derived nitrogen assimilation rate, nitrogen in tracer/nitrogen in tissue, standard deviation	DDN assim r std dev	±	Shi, Tuo
17	δ13C	δ13C	‰ PDB	Shi, Tuo
18	δ13C, standard deviation	δ13C std dev	±	Shi, Tuo
19	δ15N	δ15N	‰ air	Shi, Tuo
20	δ15N, standard deviation	δ15N std dev	±	Shi, Tuo
21	pH	pH		Shi, Tuo	Potentiometric	total scale
22	pH, standard deviation	pH std dev	±	Shi, Tuo	Potentiometric	total scale
23	Alkalinity, total	AT	µmol/l	Shi, Tuo	Potentiometric titration
24	Alkalinity, total, standard deviation	AT std dev	±	Shi, Tuo	Potentiometric titration
25	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Shi, Tuo	Calculated using CO2SYS
26	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Shi, Tuo	Calculated using CO2SYS
27	Carbon dioxide	CO2	µmol/kg	Shi, Tuo	Calculated using CO2SYS
28	Carbon dioxide, standard deviation	CO2 std dev	±	Shi, Tuo	Calculated using CO2SYS
29	Bicarbonate ion	[HCO3]-	µmol/kg	Shi, Tuo	Calculated using CO2SYS
30	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Shi, Tuo	Calculated using CO2SYS
31	Carbonate ion	[CO3]2-	µmol/kg	Shi, Tuo	Calculated using CO2SYS
32	Carbonate ion, standard deviation	[CO3]2- std dev	±	Shi, Tuo	Calculated using CO2SYS
33	Aragonite saturation state	Omega Arg		Shi, Tuo	Calculated using CO2SYS
34	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Shi, Tuo	Calculated using CO2SYS
35	Temperature, water	Temp	°C	Shi, Tuo
36	Temperature, water, standard deviation	Temp std dev	±	Shi, Tuo
37	Salinity	Sal		Shi, Tuo
38	Salinity, standard deviation	Sal std dev	±	Shi, Tuo
39	Phosphate	[PO4]3-	µmol/l	Shi, Tuo
40	Phosphate, standard deviation	[PO4]3- std dev	±	Shi, Tuo
41	Nitrite	[NO2]-	µmol/l	Shi, Tuo
42	Nitrite, standard deviation	[NO2]- std dev	±	Shi, Tuo
43	Nitrate	[NO3]-	µmol/l	Shi, Tuo
44	Silicate	Si(OH)4	µmol/l	Shi, Tuo
45	Silicate, standard deviation	Si(OH)4 std dev	±	Shi, Tuo
46	Ammonium	[NH4]+	µmol/l	Shi, Tuo
47	Ammonium, standard deviation	[NH4]+ std dev	±	Shi, Tuo
48	Alkalinity, total	AT	µmol/kg	Shi, Tuo
49	Carbonate system computation flag	CSC flag		Shi, Tuo	Calculated using seacarb after Nisumaa et al. (2010)
50	Carbon dioxide	CO2	µmol/kg	Shi, Tuo	Calculated using seacarb after Nisumaa et al. (2010)
51	Carbon dioxide, standard deviation	CO2 std dev	±	Shi, Tuo	Calculated using seacarb after Orr et al. (2018)
52	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Shi, Tuo	Calculated using seacarb after Nisumaa et al. (2010)
53	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Shi, Tuo	Calculated using seacarb after Orr et al. (2018)
54	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Shi, Tuo	Calculated using seacarb after Nisumaa et al. (2010)
55	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Shi, Tuo	Calculated using seacarb after Orr et al. (2018)
56	Bicarbonate ion	[HCO3]-	µmol/kg	Shi, Tuo	Calculated using seacarb after Nisumaa et al. (2010)
57	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Shi, Tuo	Calculated using seacarb after Orr et al. (2018)
58	Carbonate ion	[CO3]2-	µmol/kg	Shi, Tuo	Calculated using seacarb after Nisumaa et al. (2010)
59	Carbonate ion, standard deviation	[CO3]2- std dev	±	Shi, Tuo	Calculated using seacarb after Orr et al. (2018)
60	Carbon, inorganic, dissolved	DIC	µmol/kg	Shi, Tuo	Calculated using seacarb after Nisumaa et al. (2010)
61	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Shi, Tuo	Calculated using seacarb after Orr et al. (2018)
62	Aragonite saturation state	Omega Arg		Shi, Tuo	Calculated using seacarb after Nisumaa et al. (2010)
63	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Shi, Tuo	Calculated using seacarb after Orr et al. (2018)
64	Calcite saturation state	Omega Cal		Shi, Tuo	Calculated using seacarb after Nisumaa et al. (2010)
65	Calcite saturation state, standard deviation	Omega Cal std dev	±	Shi, Tuo	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

555 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Treat	7 Y	8 Y std dev [±]	9 Fv/Fm	10 Fv/Fm std dev [±]	11 Calc rate [%/week]	12 Calc rate std dev [±]	13 PFC assim r [nmol/µmol/day]	14 PFC assim r std dev [±]	15 DDN assim r [nmol/µmol/day]	16 DDN assim r std dev [±]	17 δ13C [‰ PDB]	18 δ13C std dev [±]	19 δ15N [‰ air]	20 δ15N std dev [±]	21 pH (total scale, Potentiometric)	22 pH std dev [±] (total scale, Potentiometric)	23 AT [µmol/l] (Potentiometric titration)	24 AT std dev [±] (Potentiometric titration)	25 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	26 pCO2 std dev [±] (Calculated using CO2SYS)	27 CO2 [µmol/kg] (Calculated using CO2SYS)	28 CO2 std dev [±] (Calculated using CO2SYS)	29 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	30 [HCO3]- std dev [±] (Calculated using CO2SYS)	31 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	32 [CO3]2- std dev [±] (Calculated using CO2SYS)	33 Omega Arg (Calculated using CO2SYS)	34 Omega Arg std dev [±] (Calculated using CO2SYS)	35 Temp [°C]	36 Temp std dev [±]	37 Sal	38 Sal std dev [±]	39 [PO4]3- [µmol/l]	40 [PO4]3- std dev [±]	41 [NO2]- [µmol/l]	42 [NO2]- std dev [±]	43 [NO3]- [µmol/l]	44 Si(OH)4 [µmol/l]	45 Si(OH)4 std dev [±]	46 [NH4]+ [µmol/l]	47 [NH4]+ std dev [±]	48 AT [µmol/kg]	49 CSC flag (Calculated using seacarb afte...)	50 CO2 [µmol/kg] (Calculated using seacarb afte...)	51 CO2 std dev [±] (Calculated using seacarb afte...)	52 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	53 fCO2 std dev [±] (Calculated using seacarb afte...)	54 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	55 pCO2 std dev [±] (Calculated using seacarb afte...)	56 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	57 [HCO3]- std dev [±] (Calculated using seacarb afte...)	58 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	59 [CO3]2- std dev [±] (Calculated using seacarb afte...)	60 DIC [µmol/kg] (Calculated using seacarb afte...)	61 DIC std dev [±] (Calculated using seacarb afte...)	62 Omega Arg (Calculated using seacarb afte...)	63 Omega Arg std dev [±] (Calculated using seacarb afte...)	64 Omega Cal (Calculated using seacarb afte...)	65 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Galaxea fascicularis (coral)	207366	marinespecies.org	0	pH=8.1	0.57	0.01	0.66	0.01			6.80	0.83	0.17	0.03	-27.97	2.30	4.59	0.13	8.11	0.01	2610.25	45.75	497.42	9.05	13.44	0.24	2030.49	36.95	241.69	4.39	5.84	0.01	27	0.3	34	0.1	0.03	0.01	0.45	0.43	<0.05	0.18	0.18	0.35	0.35	2554.148820	8	10.01	0.40	368.71	14.58	369.86	14.63	1889.42	39.77	276.72	10.38	2176.15	41.37	4.46	0.27	6.74	0.40
laboratory	Galaxea fascicularis (coral)	207366	marinespecies.org	0	pH=7.7	0.55	0.02	0.65	0.01			6.79	0.82	0.16	0.04	-28.41	0.02	4.70	1.43	7.69	0.01	2608.00	41.98	1115.37	18.30	30.13	0.49	2282.09	37.51	136.14	2.23	3.29	0.05	27	0.3	34	0.1	0.03	0.01	0.49	0.33	<0.05	0.36	0.18	0.51	0.33	2551.947179	8	31.39	1.13	1155.73	41.10	1159.34	41.22	2251.67	38.40	125.38	5.24	2408.43	40.05	2.02	0.13	3.06	0.19
laboratory	Galaxea fascicularis (coral)	207366	marinespecies.org	0	pH=7.4	0.57	0.02	0.66	0.01			6.55	0.77	0.17	0.03	-29.06	0.35	4.69	0.38	7.39	0.02	2657.50	42.52	2419.20	39.12	65.36	1.05	2480.71	40.12	74.17	1.19	1.79	0.02	27	0.3	34	0.1	0.05	0.01	0.58	0.32	<0.05	0.51	0.26	0.84	0.45	2600.383293	8	67.81	3.71	2496.92	136.30	2504.74	136.73	2438.10	40.31	68.04	3.91	2573.96	42.37	1.10	0.08	1.66	0.12
laboratory	Galaxea fascicularis (coral)	207366	marinespecies.org	14	pH=8.1	0.58	0.02	0.67	0.01	0.22	0.05									8.11	0.01	2610.25	45.75	497.42	9.05	13.44	0.24	2030.49	36.95	241.69	4.39	5.84	0.01	27	0.3	34	0.1	0.03	0.01	0.45	0.43	<0.05	0.18	0.18	0.35	0.35	2554.148820	8	10.01	0.40	368.71	14.58	369.86	14.63	1889.42	39.77	276.72	10.38	2176.15	41.37	4.46	0.27	6.74	0.40
laboratory	Galaxea fascicularis (coral)	207366	marinespecies.org	14	pH=7.7	0.56	0.01	0.66	0.01	0.09	0.05									7.69	0.01	2608.00	41.98	1115.37	18.30	30.13	0.49	2282.09	37.51	136.14	2.23	3.29	0.05	27	0.3	34	0.1	0.03	0.01	0.49	0.33	<0.05	0.36	0.18	0.51	0.33	2551.947179	8	31.39	1.13	1155.73	41.10	1159.34	41.22	2251.67	38.40	125.38	5.24	2408.43	40.05	2.02	0.13	3.06	0.19
laboratory	Galaxea fascicularis (coral)	207366	marinespecies.org	14	pH=7.4	0.56	0.01	0.65	0.01	-0.01	0.03									7.39	0.02	2657.50	42.52	2419.20	39.12	65.36	1.05	2480.71	40.12	74.17	1.19	1.79	0.02	27	0.3	34	0.1	0.05	0.01	0.58	0.32	<0.05	0.51	0.26	0.84	0.45	2600.383293	8	67.81	3.71	2496.92	136.30	2504.74	136.73	2438.10	40.31	68.04	3.91	2573.96	42.37	1.10	0.08	1.66	0.12
laboratory	Galaxea fascicularis (coral)	207366	marinespecies.org	28	pH=8.1	0.58	0.01	0.67	0.01	0.18	0.05	9.24	0.99	0.16	0.02	-27.78	0.40	5.64	0.26	8.11	0.01	2610.25	45.75	497.42	9.05	13.44	0.24	2030.49	36.95	241.69	4.39	5.84	0.01	27	0.3	34	0.1	0.03	0.01	0.45	0.43	<0.05	0.18	0.18	0.35	0.35	2554.148820	8	10.01	0.40	368.71	14.58	369.86	14.63	1889.42	39.77	276.72	10.38	2176.15	41.37	4.46	0.27	6.74	0.40
laboratory	Galaxea fascicularis (coral)	207366	marinespecies.org	28	pH=7.7	0.55	0.02	0.63	0.01	-0.04	0.03	6.84	1.04	0.13	0.03	-28.75	0.58	4.80	0.41	7.69	0.01	2608.00	41.98	1115.37	18.30	30.13	0.49	2282.09	37.51	136.14	2.23	3.29	0.05	27	0.3	34	0.1	0.03	0.01	0.49	0.33	<0.05	0.36	0.18	0.51	0.33	2551.947179	8	31.39	1.13	1155.73	41.10	1159.34	41.22	2251.67	38.40	125.38	5.24	2408.43	40.05	2.02	0.13	3.06	0.19
laboratory	Galaxea fascicularis (coral)	207366	marinespecies.org	28	pH=7.4	0.49	0.02	0.63	0.02	-0.14	0.13	7.11	1.95	0.03	0.02	-30.56	0.40	4.45	0.06	7.39	0.02	2657.50	42.52	2419.20	39.12	65.36	1.05	2480.71	40.12	74.17	1.19	1.79	0.02	27	0.3	34	0.1	0.05	0.01	0.58	0.32	<0.05	0.51	0.26	0.84	0.45	2600.383293	8	67.81	3.71	2496.92	136.30	2504.74	136.73	2438.10	40.31	68.04	3.91	2573.96	42.37	1.10	0.08	1.66	0.12