Miksch, Lukas; Gutow, Lars; Saborowski, Reinhard (2021): Hydrolysis rates of bio-based polymers [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.931466
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Published: 2021-05-14 • DOI registered: 2021-06-15
Abstract:
Bio-degradation assays were performed for bio-based polymers under environmental conditions with controlled temperature and pH using pH Stat titration. Suspensions of PLA and PBS microparticles, produced by e.g. cryo-milling, were incubated with proteolytic and lipolytic enzymes. Rates of hydrolysis, as determined by counter-titration with a diluted base (NaOH), was recorded for two hours. The thermal profile of PLA hydrolysis by protease was investigated. All measurements were conducted under controlled laboratory conditions.
Keyword(s):
Supplement to:
Miksch, Lukas; Gutow, Lars; Saborowski, Reinhard (2021): pH-Stat Titration: A Rapid Assay for Enzymatic Degradability of Bio-Based Polymers. Polymers, 13(6), 860, https://doi.org/10.3390/polym13060860
Project(s):
Funding:
Horizon 2020 of the European Commission (H2020), grant/award no. 860407: Developing and Implementing Sustainability-Based Solutions for Bio-Based Plastic Production and Use to Preserve Land and Sea Environmental Quality in Europe
Comment:
The columns headings are abbreviated and mean the following. Min: Duration of the titration in minutes; ml: Volume of added NaOH. Unless otherwise specified, c (NaOH) = 10 mM; pH: pH value of the suspension, set to 8.2; °C: Temperature of the suspension.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | File content | Content | Miksch, Lukas | |||
| 2 | Binary Object | Binary | Miksch, Lukas | |||
| 3 | Binary Object (File Size) | Binary (Size) | Bytes | Miksch, Lukas |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Basic curation (CurationLevelB)
Size:
12 data points
Data
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| 1 Content | 2 Binary | 3 Binary (Size) [Bytes] |
|---|---|---|
| hydrolysis rates of different enzymes in seawater without polymer particles | Enzyme_Blanks.xlsx | 151.7 kBytes |
| hydrolysis rates of Polylactic acid suspensions incubated with different concentrations of protease (Bacillus licheniformis) | Enzyme_Concentration_PLA_Protease.xlsx | 95.7 kBytes |
| hydrolysis rates of Polybutylene succinate suspensions incubated with protease (Bacillus licheniformis), proteinase K (Tritirachium album) and lipase (Candida antarctica) | Enzyme_Specificity_PBS.xlsx | 57.9 kBytes |
| hydrolysis rates of Polylactic acid suspensions incubated with protease (Bacillus licheniformis), proteinase K (Tritirachium album) and lipase (Candida antarctica) | Enzyme_Specificity_PLA.xlsx | 57.1 kBytes |
| data of pH change in seawater or Polylactic acid suspensions over time with different concentrations of NaOH | Leakage_PLA_SW.xlsx | 35.1 kBytes |
| hydrolysis rates of Polylactic acid suspensions incubated with protease (Bacillus licheniformis) at different temperatures | Temperature_PLA_Protease.xlsx | 132.1 kBytes |