Citation:

PANGAEA.930934

Name: Seawater carbonate chemistry and estuarine dissolved organic carbon export
Published: 2020
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Entire community; Laboratory experiment; Other studied parameter or process; Primary production/Photosynthesis; Respiration; Soft-bottom community; South Pacific; Temperate; Temperature

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Abstract:

Relative to their surface area, estuaries make a disproportionately large contribution of dissolved organic carbon (DOC) to the global carbon cycle, but it is unknown how this will change under a future climate. As such, the response of DOC fluxes from microbially dominated unvegetated sediments to individual and combined future climate stressors of temperature change (from delta −3 to delta +5 °C compared to ambient mean temperatures) and ocean acidification (OA, 2*current CO2 partial pressure, pCO2) was investigated ex situ. Warming alone increased sediment heterotrophy, resulting in a proportional increase in sediment DOC uptake; sediments became net sinks of DOC (3.5 to 8.8 mmol C/m**2/d) at warmer temperatures (delta +3 and delta +5 °C, respectively). This temperature response changed under OA conditions, with sediments becoming more autotrophic and a greater sink of DOC (up to 4* greater than under current pCO2 conditions). This response was attributed to the stimulation of heterotrophic bacteria with the autochthonous production of labile organic matter by microphytobenthos. Extrapolating these results to the global area of unvegetated subtidal estuarine sediments, we find that the future climate of warming (delta +3 °C) and OA may decrease estuarine export of DOC by ∼ 80 % (150 Tg C/yr) and have a disproportionately large impact on the global DOC budget.

Keyword(s):

Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Entire community; Laboratory experiment; Other studied parameter or process; Primary production/Photosynthesis; Respiration; Soft-bottom community; South Pacific; Temperate; Temperature

Supplement to:

Simone, Michelle; Schulz, Kai Georg; Oakes, Joanne; Eyre, Bradley D (2021): Warming and ocean acidification may decrease estuarine dissolved organic carbon export to the ocean. Biogeosciences, 18(5), 1823-1838, https://doi.org/10.5194/bg-18-1823-2021

Original version:

Simone, Michelle; Schulz, Kai; Oakes, Joanne; Eyre, Bradley D (2020): Oxygen and carbon fluxes from shallow unvegetated sediments in the Clarence Estuary, NSW, Australia under warming and ocean acidification conditions. PANGAEA, https://doi.org/10.1594/PANGAEA.924460

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -29.403500 * Longitude: 153.324000

Event(s):

Clarence_River_estuary * Latitude: -29.403500 * Longitude: 153.324000 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-04-30.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Simone, Michelle		study
2	Treatment	Treat		Simone, Michelle		Treatment 1
3	Treatment	Treat		Simone, Michelle		Treatment 2
4	Time point, descriptive	Time point		Simone, Michelle
5	Replicates	Repl	#	Simone, Michelle
6	DEPTH, sediment/rock	Depth sed	m	Simone, Michelle		Geocode
7	DEPTH, water	Depth water	m	Simone, Michelle		Geocode
8	Surface area	Surface	m²	Simone, Michelle		Of core
9	Volume	Vol	l	Simone, Michelle		Of water in the core
10	Time in minutes	Time	min	Simone, Michelle		Sampling time
11	Salinity	Sal		Simone, Michelle
12	Temperature, water	Temp	°C	Simone, Michelle
13	Oxygen saturation	O2 sat	mg/l	Simone, Michelle
14	Carbon, inorganic, dissolved	DIC	µmol/l	Simone, Michelle	AIRICA analyzer (Miranda)
15	Carbon, inorganic, dissolved	DIC	µmol/kg	Simone, Michelle
16	Carbon, organic, dissolved	DOC	µmol/l	Simone, Michelle
17	pH	pH		Simone, Michelle	Potentiometric	free scale
18	Respiration rate, oxygen	Resp O2	mmol/m²/h	Simone, Michelle		Actual
19	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Simone, Michelle		Actual
20	Respiration rate, oxygen	Resp O2	mmol/m²/h	Simone, Michelle		Scaled
21	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Simone, Michelle		Scaled
22	Net primary production of oxygen	NPP O2	mmol/m²/h	Simone, Michelle		Actual
23	Net primary production of oxygen, standard deviation	NPP O2 std dev	±	Simone, Michelle		Actual
24	Net primary production of oxygen	NPP O2	mmol/m²/h	Simone, Michelle		Scaled
25	Net primary production of oxygen, standard deviation	NPP O2 std dev	±	Simone, Michelle		Scaled
26	Gross primary production of oxygen	GPP O2	mmol/m²/h	Simone, Michelle
27	Gross primary production of oxygen, standard deviation	GPP O2 std dev	±	Simone, Michelle
28	Production/respiration ratio	P/resp		Simone, Michelle
29	Production/respiration ratio, standard deviation	P/R std dev	±	Simone, Michelle
30	Carbon, inorganic, dissolved, flux	DIC flux	mmol/m²/h	Simone, Michelle		Dark
31	Carbon, inorganic, dissolved, flux, standard deviation	DIC flux std dev	±	Simone, Michelle		Dark
32	Carbon, inorganic, dissolved, flux	DIC flux	mmol/m²/h	Simone, Michelle		Light
33	Carbon, inorganic, dissolved, flux, standard deviation	DIC flux std dev	±	Simone, Michelle		Light
34	Carbon, organic, dissolved, flux	DOC flux	µmol/m²/h	Simone, Michelle		Dark
35	Carbon, organic, dissolved, flux, standard deviation	DOC flux std dev	±	Simone, Michelle		Dark
36	Carbon, organic, dissolved, flux	DOC flux	µmol/m²/h	Simone, Michelle		Light
37	Carbon, organic, dissolved, flux, standard deviation	DOC flux std dev	±	Simone, Michelle		Light
38	Nitrogen, inorganic, dissolved	DIN	µmol/l	Simone, Michelle		Start
39	Nitrogen, inorganic, dissolved, standard deviation	DIN std dev	±	Simone, Michelle		End
40	Nitrogen, inorganic, dissolved	DIN	µmol/l	Simone, Michelle		Start
41	Nitrogen, inorganic, dissolved, standard deviation	DIN std dev	±	Simone, Michelle		End
42	Salinity	Sal		Simone, Michelle
43	Temperature, water	Temp	°C	Simone, Michelle
44	Temperature, water, standard deviation	Temp std dev	±	Simone, Michelle
45	pH	pH		Simone, Michelle	Potentiometric	free scale
46	pH, standard deviation	pH std dev	±	Simone, Michelle	Potentiometric	free scale
47	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Simone, Michelle	Calculated using CO2SYS
48	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Simone, Michelle	Calculated using CO2SYS
49	Bicarbonate ion	[HCO3]-	µmol/kg	Simone, Michelle	Calculated using CO2SYS
50	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Simone, Michelle	Calculated using CO2SYS
51	Carbonate ion	[CO3]2-	µmol/kg	Simone, Michelle	Calculated using CO2SYS
52	Carbonate ion, standard deviation	[CO3]2- std dev	±	Simone, Michelle	Calculated using CO2SYS
53	Alkalinity, total	AT	µmol/kg	Simone, Michelle	Calculated using CO2SYS
54	Alkalinity, total, standard deviation	AT std dev	±	Simone, Michelle	Calculated using CO2SYS
55	Carbon, inorganic, dissolved	DIC	µmol/kg	Simone, Michelle	AIRICA analyzer (Miranda)
56	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Simone, Michelle	AIRICA analyzer (Miranda)
57	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
59	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
61	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
63	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
65	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
66	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2737 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Treat (Treatment 1)	3 Treat (Treatment 2)	4 Time point	5 Repl [#]	6 Depth sed [m]	7 Depth water [m]	8 Surface [m²] (Of core)	9 Vol [l] (Of water in the core)	10 Time [min] (Sampling time)	11 Sal	12 Temp [°C]	13 O2 sat [mg/l]	14 DIC [µmol/l] (AIRICA analyzer (Miranda))	15 DIC [µmol/kg]	16 DOC [µmol/l]	17 pH (free scale, Potentiometric)	18 Resp O2 [mmol/m²/h] (Actual)	19 Resp O2 std dev [±] (Actual)	20 Resp O2 [mmol/m²/h] (Scaled)	21 Resp O2 std dev [±] (Scaled)	22 NPP O2 [mmol/m²/h] (Actual)	23 NPP O2 std dev [±] (Actual)	24 NPP O2 [mmol/m²/h] (Scaled)	25 NPP O2 std dev [±] (Scaled)	26 GPP O2 [mmol/m²/h]	27 GPP O2 std dev [±]	28 P/resp	29 P/R std dev [±]	30 DIC flux [mmol/m²/h] (Dark)	31 DIC flux std dev [±] (Dark)	32 DIC flux [mmol/m²/h] (Light)	33 DIC flux std dev [±] (Light)	34 DOC flux [µmol/m²/h] (Dark)	35 DOC flux std dev [±] (Dark)	36 DOC flux [µmol/m²/h] (Light)	37 DOC flux std dev [±] (Light)	38 DIN [µmol/l] (Start)	39 DIN std dev [±] (End)	40 DIN [µmol/l] (Start)	41 DIN std dev [±] (End)	42 Sal	43 Temp [°C]	44 Temp std dev [±]	45 pH (free scale, Potentiometric)	46 pH std dev [±] (free scale, Potentiometric)	47 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	48 pCO2 std dev [±] (Calculated using CO2SYS)	49 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	50 [HCO3]- std dev [±] (Calculated using CO2SYS)	51 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	52 [CO3]2- std dev [±] (Calculated using CO2SYS)	53 AT [µmol/kg] (Calculated using CO2SYS)	54 AT std dev [±] (Calculated using CO2SYS)	55 DIC [µmol/kg] (AIRICA analyzer (Miranda))	56 DIC std dev [±] (AIRICA analyzer (Miranda))	57 CSC flag (Calculated using seacarb afte...)	58 pH (total scale, Calculated using...)	59 CO2 [µmol/kg] (Calculated using seacarb afte...)	60 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	61 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	62 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	63 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	64 AT [µmol/kg] (Calculated using seacarb afte...)	65 Omega Arg (Calculated using seacarb afte...)	66 Omega Cal (Calculated using seacarb afte...)
laboratory	current pCO2	plus 5 centigrade	dark start	1	0.190	0.260	0.007089	1.843140	90.000	24.4	27.20	6.864	1857.583	1830.681	267.883	8.166																																								27	8.07	10.67	375.94	377.11	1648.01	172.00	2059.56	2.90	4.53
laboratory	current pCO2	plus 5 centigrade	dark start	2	0.225	0.225	0.007089	1.595025	96.545	24.4	27.10	6.654	1869.984	1842.847	295.496	8.092																																								27	7.99	12.92	453.93	455.34	1682.15	147.78	2036.34	2.49	3.89
laboratory	current pCO2	plus 5 centigrade	dark start	3	0.245	0.205	0.007089	1.453245	103.091	24.4	27.00	6.535	1870.733	1843.529	284.994	8.067																																								27	7.97	13.76	482.50	484.01	1690.03	139.74	2025.24	2.35	3.68
laboratory	current pCO2	plus 5 centigrade	dark end	1	0.190	0.260	0.007089	1.843140	450.000	24.4	27.30	5.895	1884.206	1856.975	259.632	8.015																																								27	7.92	15.69	554.31	556.04	1714.49	126.79	2018.70	2.14	3.34
laboratory	current pCO2	plus 5 centigrade	dark end	2	0.225	0.225	0.007089	1.595025	455.727	24.4	27.20	5.605	1907.641	1880.015	265.773	7.957																																								27	7.86	18.28	644.15	646.16	1748.71	113.02	2019.83	1.91	2.98
laboratory	current pCO2	plus 5 centigrade	dark end	3	0.245	0.205	0.007089	1.453245	461.455	24.4	27.30	5.228	1914.344	1886.678	265.032	7.934																																								27	7.84	19.40	685.37	687.51	1759.30	107.97	2018.31	1.82	2.85
laboratory	current pCO2	plus 5 centigrade	light end	1	0.190	0.260	0.007089	1.843140	1114.091	24.4	27.40	5.714	1905.192	1877.715	257.257	8.006																																								27	7.91	16.17	572.68	574.46	1735.29	126.25	2037.70	2.13	3.33
laboratory	current pCO2	plus 5 centigrade	light end	2	0.225	0.225	0.007089	1.595025	1119.545	24.4	27.40	4.547	1978.844	1950.304	272.752	7.870																																								27	7.77	23.36	827.25	829.82	1829.76	97.18	2061.98	1.64	2.56
laboratory	current pCO2	plus 5 centigrade	light end	3	0.245	0.205	0.007089	1.453245	1125.000	24.4	27.40	4.361	1990.054	1961.353	285.783	7.951																																								27	7.85	19.33	684.55	686.68	1825.17	116.84	2103.53	1.97	3.08
laboratory	current pCO2	plus 3 centigrade	dark start	1	0.225	0.225	0.007089	1.595025	109.636	24.4	25.00	6.791	1873.264	1844.939	273.233	8.062																																								27	7.97	14.33	477.67	479.20	1699.56	131.04	2014.59	2.18	3.43
laboratory	current pCO2	plus 3 centigrade	dark start	2	0.230	0.220	0.007089	1.559580	116.182	24.4	25.00	6.807	1876.132	1847.763	204.073	8.083																																								27	7.99	13.65	454.98	456.44	1696.96	137.16	2026.36	2.28	3.59
laboratory	current pCO2	plus 3 centigrade	dark start	3	0.250	0.200	0.007089	1.417800	122.727	24.4	24.90	6.734	1874.105	1845.714	214.651	8.082																																								27	7.99	13.69	455.00	456.45	1695.67	136.36	2023.26	2.27	3.56
laboratory	current pCO2	plus 3 centigrade	dark end	1	0.225	0.225	0.007089	1.595025	467.182	24.4	25.90	5.788	1911.028	1882.622	252.393	7.965																																								27	7.87	18.29	623.79	625.76	1753.33	111.00	2019.84	1.86	2.91
laboratory	current pCO2	plus 3 centigrade	dark end	2	0.230	0.220	0.007089	1.559580	472.909	24.4	25.90	5.877	1908.281	1879.917	187.840	7.988																																								27	7.89	17.26	588.58	590.44	1746.00	116.66	2025.96	1.95	3.06
laboratory	current pCO2	plus 3 centigrade	dark end	3	0.250	0.200	0.007089	1.417800	478.636	24.4	25.90	5.676	1907.098	1878.751	213.066	8.032																																								27	7.94	15.50	528.80	530.47	1735.02	128.22	2042.34	2.15	3.36
laboratory	current pCO2	plus 3 centigrade	light end	1	0.225	0.225	0.007089	1.595025	1097.727	24.4	26.00	4.783	1972.950	1943.680	270.149	7.878																																								27	7.78	23.25	794.83	797.35	1825.59	94.85	2052.64	1.59	2.49
laboratory	current pCO2	plus 3 centigrade	light end	2	0.230	0.220	0.007089	1.559580	1103.182	24.4	25.90	5.907	1940.174	1911.335	198.002	7.991																																								27	7.90	17.45	595.26	597.15	1774.71	119.18	2059.90	1.99	3.13
laboratory	current pCO2	plus 3 centigrade	light end	3	0.250	0.200	0.007089	1.417800	1108.636	24.4	25.90	5.354	1951.441	1922.435	212.785	7.895																																								27	7.80	22.12	754.40	756.79	1803.23	97.08	2035.98	1.62	2.55
laboratory	current pCO2	control	dark start	1	0.220	0.230	0.007089	1.630470	129.273	24.4	23.10	6.951	1890.207	1860.643	207.441	8.074																																								27	7.99	14.45	458.19	459.69	1717.67	128.53	2026.76	2.11	3.34
laboratory	current pCO2	control	dark start	2	0.220	0.230	0.007089	1.630470	135.818	24.4	23.10	6.908	1887.370	1857.851	211.644	8.047																																								27	7.96	15.39	488.17	489.77	1721.31	121.15	2012.99	1.99	3.15
laboratory	current pCO2	control	dark start	3	0.220	0.230	0.007089	1.630470	142.364	24.4	23.10	6.929	1891.223	1861.644	174.762	8.095																																								27	8.01	13.72	435.05	436.48	1713.26	134.67	2036.84	2.21	3.50
laboratory	current pCO2	control	dark end	1	0.220	0.230	0.007089	1.630470	484.364	24.4	23.10	6.086	1925.226	1895.115	190.550	8.007																																								27	7.92	17.32	549.20	551.00	1764.63	113.17	2036.56	1.86	2.94
laboratory	current pCO2	control	dark end	2	0.220	0.230	0.007089	1.630470	490.091	24.4	23.10	6.064	1914.731	1884.784	196.961	8.069																																								27	7.98	14.81	469.75	471.29	1741.15	128.82	2050.32	2.12	3.35
laboratory	current pCO2	control	dark end	3	0.220	0.230	0.007089	1.630470	495.818	24.4	23.10	6.129	1921.265	1891.215	162.357	8.099																																								27	8.01	13.80	437.75	439.18	1739.45	137.97	2070.03	2.27	3.58
laboratory	current pCO2	control	light end	1	0.220	0.230	0.007089	1.630470	1081.364	24.4	23.30	6.833	1910.013	1880.241	218.756	8.004																																								27	7.92	17.25	550.03	551.82	1750.78	112.21	2020.72	1.85	2.92
laboratory	current pCO2	control	light end	2	0.220	0.230	0.007089	1.630470	1086.818	24.4	23.30	6.668	1908.849	1879.095	219.940	8.009																																								27	7.92	17.04	543.19	544.97	1748.71	113.35	2021.38	1.87	2.95
laboratory	current pCO2	control	light end	3	0.220	0.230	0.007089	1.630470	1092.273	24.4	23.40	6.969	1908.184	1878.492	201.248	8.068																																								27	7.98	14.74	471.31	472.85	1734.64	129.11	2044.58	2.13	3.36
laboratory	current pCO2	minus 3 centigrade	dark start	1	0.230	0.220	0.007089	1.559580	148.909	24.4	21.00	7.220	1888.760	1858.200	183.223	8.082																																								27	8.00	14.61	437.86	439.33	1720.49	123.09	2017.27	2.00	3.18
laboratory	current pCO2	minus 3 centigrade	dark start	2	0.230	0.220	0.007089	1.559580	155.455	24.4	20.90	7.163	1887.126	1856.546	182.076	8.053																																								27	7.97	15.71	469.31	470.89	1725.79	115.04	2003.54	1.87	2.97
laboratory	current pCO2	minus 3 centigrade	dark start	3	0.220	0.230	0.007089	1.630470	162.000	24.4	21.00	7.160	1893.514	1862.878	184.187	8.097																																								27	8.02	14.12	423.19	424.62	1721.28	127.47	2028.32	2.07	3.29
laboratory	current pCO2	minus 3 centigrade	dark end	1	0.230	0.220	0.007089	1.559580	501.545	24.4	21.00	6.580	1904.803	1873.983	191.416	8.066																																								27	7.99	15.36	460.08	461.63	1738.95	119.67	2027.25	1.94	3.09
laboratory	current pCO2	minus 3 centigrade	dark end	2	0.230	0.220	0.007089	1.559580	507.273	24.4	21.00	6.336	1900.453	1869.705	189.124	8.100																																								27	8.02	14.08	421.76	423.18	1726.89	128.74	2036.75	2.09	3.32
laboratory	current pCO2	minus 3 centigrade	dark end	3	0.220	0.230	0.007089	1.630470	513.000	24.4	21.00	6.461	1917.145	1886.126	194.149	8.114																																								27	8.03	13.75	411.82	413.21	1738.72	133.66	2059.80	2.17	3.45
laboratory	current pCO2	minus 3 centigrade	light end	1	0.230	0.220	0.007089	1.559580	1065.000	24.4	20.90	7.049	1896.926	1866.187	177.157	8.037																																								27	7.96	16.40	489.96	491.61	1738.03	111.76	2007.76	1.82	2.88
laboratory	current pCO2	minus 3 centigrade	light end	2	0.230	0.220	0.007089	1.559580	1070.455	24.4	21.30	7.239	1897.309	1866.753	183.641	8.077																																								27	8.00	14.80	447.15	448.65	1728.65	123.30	2025.63	2.01	3.19
laboratory	current pCO2	minus 3 centigrade	light end	3	0.220	0.230	0.007089	1.630470	1075.909	24.4	21.00	7.121	1904.229	1873.419	194.271	8.063																																								27	7.98	15.43	462.37	463.93	1738.91	119.07	2025.80	1.93	3.07
laboratory	future pCO2	plus 5 centigrade	dark start	1	0.210	0.240	0.007089	1.701360	70.000	17.7	28.10	7.499	1311.190	1298.965	619.413	7.662																																								27	7.58	27.04	941.35	944.25	1236.72	35.20	1323.91	0.61	0.99
laboratory	future pCO2	plus 5 centigrade	dark start	2	0.210	0.240	0.007089	1.701360	78.571	17.7	28.00	7.569	1318.957	1306.620	621.741	7.629																																								27	7.54	29.39	1020.90	1024.05	1244.50	32.73	1325.54	0.57	0.92
laboratory	future pCO2	plus 5 centigrade	dark start	3	0.200	0.250	0.007089	1.772250	87.143	17.7	27.80	7.488	1321.053	1308.617	661.408	7.649																																								27	7.57	28.12	971.88	974.89	1246.31	34.19	1330.91	0.59	0.96
laboratory	future pCO2	plus 5 centigrade	dark end	1	0.210	0.240	0.007089	1.701360	435.000	17.7	27.90	6.619	1340.528	1327.948	591.799	7.575																																								27	7.49	33.85	1172.68	1176.31	1264.79	29.31	1337.23	0.51	0.82
laboratory	future pCO2	plus 5 centigrade	dark end	2	0.210	0.240	0.007089	1.701360	441.500	17.7	27.85	6.795	1357.827	1345.065	594.506	7.538																																								27	7.45	37.30	1290.75	1294.75	1280.53	27.23	1347.73	0.47	0.76
laboratory	future pCO2	plus 5 centigrade	dark end	3	0.200	0.250	0.007089	1.772250	448.000	17.7	27.80	6.696	1354.251	1341.502	650.001	7.528																																								27	7.44	38.10	1316.59	1320.66	1276.91	26.49	1342.34	0.46	0.74
laboratory	future pCO2	plus 5 centigrade	light end	1	0.210	0.240	0.007089	1.701360	1137.143	17.7	27.90	6.200	1340.694	1328.113	576.874	7.458																																								27	7.37	44.17	1530.31	1535.05	1261.60	22.34	1316.95	0.39	0.63
laboratory	future pCO2	plus 5 centigrade	light end	2	0.210	0.240	0.007089	1.701360	1143.571	17.7	28.00	6.434	1474.042	1460.254	583.053	7.436																																								27	7.35	50.99	1770.81	1776.29	1385.87	23.40	1442.85	0.40	0.66
laboratory	future pCO2	plus 5 centigrade	light end	3	0.200	0.250	0.007089	1.772250	1150.000	17.7	27.90	6.175	1406.982	1393.779	636.974	7.409																																								27	7.33	51.76	1793.30	1798.85	1321.11	20.91	1372.49	0.36	0.59
laboratory	future pCO2	plus 3 centigrade	dark start	1	0.205	0.245	0.007089	1.736805	95.714	17.7	26.00	7.537	1330.437	1317.215	587.962	7.588																																								27	7.51	33.41	1103.48	1106.97	1255.52	28.29	1325.68	0.48	0.78
laboratory	future pCO2	plus 3 centigrade	dark start	2	0.210	0.240	0.007089	1.701360	104.286	17.7	25.80	7.545	1331.704	1318.394	607.750	7.773																																								27	7.69	21.84	717.72	720.00	1253.57	42.99	1359.72	0.73	1.19
laboratory	future pCO2	plus 3 centigrade	dark start	3	0.220	0.230	0.007089	1.630470	112.857	17.7	25.70	7.593	1325.341	1312.057	583.714	7.558																																								27	7.48	35.78	1172.91	1176.63	1250.22	26.05	1314.95	0.44	0.72
laboratory	future pCO2	plus 3 centigrade	dark end	1	0.205	0.245	0.007089	1.736805	454.500	17.7	25.70	6.780	1359.953	1346.323	558.703	7.659																																								27	7.58	29.11	954.24	957.27	1283.46	33.75	1366.70	0.57	0.93
laboratory	future pCO2	plus 3 centigrade	dark end	2	0.210	0.240	0.007089	1.701360	461.000	17.7	25.60	6.843	1363.151	1349.450	583.200	7.494																																								27	7.42	42.56	1391.31	1395.74	1283.83	23.06	1340.90	0.39	0.64
laboratory	future pCO2	plus 3 centigrade	dark end	3	0.220	0.230	0.007089	1.630470	467.500	17.7	25.70	6.908	1349.151	1335.629	548.859	7.679																																								27	7.60	27.55	902.99	905.86	1273.00	35.08	1359.62	0.60	0.97
laboratory	future pCO2	plus 3 centigrade	light end	1	0.205	0.245	0.007089	1.736805	1117.857	17.7	26.00	6.949	1370.660	1357.038	561.689	7.597																																								27	7.52	33.66	1111.80	1115.32	1293.57	29.81	1367.06	0.51	0.83
laboratory	future pCO2	plus 3 centigrade	light end	2	0.210	0.240	0.007089	1.701360	1124.286	17.7	26.00	7.331	1370.048	1356.432	561.961	7.673																																								27	7.59	28.24	932.69	935.64	1292.71	35.48	1380.05	0.60	0.98
laboratory	future pCO2	plus 3 centigrade	light end	3	0.220	0.230	0.007089	1.630470	1130.714	17.7	26.00	6.972	1341.977	1328.641	580.617	7.687																																								27	7.61	26.79	884.87	887.67	1265.98	35.87	1354.59	0.61	1.00
laboratory	future pCO2	control	dark start	1	0.220	0.230	0.007089	1.630470	121.429	17.7	23.30	7.680	1346.674	1332.300	583.913	7.567																																								27	7.49	36.78	1132.14	1135.85	1270.41	25.11	1332.78	0.42	0.69
laboratory	future pCO2	control	dark start	2	0.205	0.245	0.007089	1.736805	130.000	17.7	23.20	7.665	1347.154	1332.740	592.137	7.695																																								27	7.62	27.49	843.87	846.63	1271.64	33.60	1354.89	0.56	0.92
laboratory	future pCO2	control	dark start	3	0.220	0.230	0.007089	1.630470	138.571	17.7	23.20	7.765	1336.391	1322.092	587.541	7.631																																								27	7.56	31.57	969.13	972.30	1261.71	28.81	1333.29	0.48	0.79
laboratory	future pCO2	control	dark end	1	0.220	0.230	0.007089	1.630470	474.000	17.7	23.00	6.975	1374.673	1359.894	569.501	7.505																																								27	7.43	43.40	1324.94	1329.29	1294.51	21.99	1349.01	0.37	0.60
laboratory	future pCO2	control	dark end	2	0.205	0.245	0.007089	1.736805	480.500	17.7	23.00	6.950	1368.012	1353.304	575.631	7.467																																								27	7.40	47.07	1436.99	1441.71	1286.22	20.01	1335.92	0.33	0.55
laboratory	future pCO2	control	dark end	3	0.220	0.230	0.007089	1.630470	487.000	17.7	23.00	7.107	1366.627	1351.934	561.685	7.617																																								27	7.55	33.45	1021.34	1024.69	1290.15	28.33	1360.28	0.47	0.77
laboratory	future pCO2	control	light end	1	0.220	0.230	0.007089	1.630470	1060.000	17.7	23.20	7.531	1385.938	1371.110	569.903	7.828																																								27	7.76	20.74	636.61	638.70	1303.56	46.81	1418.50	0.78	1.28
laboratory	future pCO2	control	light end	2	0.205	0.245	0.007089	1.736805	1066.429	17.7	23.20	7.671	1347.577	1333.159	595.454	7.638																																								27	7.57	31.32	961.31	964.46	1272.31	29.53	1345.55	0.49	0.81
laboratory	future pCO2	control	light end	3	0.220	0.230	0.007089	1.630470	1072.857	17.7	23.20	7.910	1324.550	1310.378	581.912	7.700																																								27	7.63	26.67	818.74	821.43	1250.23	33.48	1333.42	0.56	0.92
laboratory	future pCO2	minus 3 centigrade	dark start	1	0.220	0.230	0.007089	1.630470	172.857	17.7	20.70	7.960	1291.800	1277.176	508.606	7.617																																								27	7.55	32.73	938.07	941.24	1219.54	24.90	1282.10	0.41	0.67
laboratory	future pCO2	minus 3 centigrade	dark start	2	0.225	0.225	0.007089	1.595025	181.429	17.7	20.80	7.863	1294.322	1279.699	595.027	7.583																																								27	7.52	35.40	1017.52	1020.96	1221.17	23.12	1279.27	0.38	0.63
laboratory	future pCO2	minus 3 centigrade	dark start	3	0.200	0.250	0.007089	1.772250	190.000	17.7	20.75	7.989	1288.531	1273.959	597.787	7.603																																								27	7.54	33.75	968.72	971.99	1216.17	24.04	1276.59	0.39	0.65
laboratory	future pCO2	minus 3 centigrade	dark end	1	0.220	0.230	0.007089	1.630470	513.000	17.7	20.70	7.417	1315.769	1300.872	483.416	7.700																																								27	7.63	27.60	791.12	793.79	1242.61	30.66	1319.18	0.50	0.83
laboratory	future pCO2	minus 3 centigrade	dark end	2	0.225	0.225	0.007089	1.595025	519.500	17.7	20.70	7.395	1313.735	1298.862	565.478	7.688																																								27	7.62	28.34	812.11	814.85	1240.75	29.78	1315.16	0.49	0.81
laboratory	future pCO2	minus 3 centigrade	dark end	3	0.200	0.250	0.007089	1.772250	526.000	17.7	20.80	7.513	1305.629	1290.879	564.866	7.697																																								27	7.63	27.49	790.18	792.84	1233.03	30.36	1308.95	0.50	0.82
laboratory	future pCO2	minus 3 centigrade	light end	1	0.220	0.230	0.007089	1.630470	1098.571	17.7	20.70	7.962	1310.248	1295.414	515.243	7.808																																								27	7.74	21.38	612.62	614.69	1234.94	39.10	1332.39	0.64	1.06
laboratory	future pCO2	minus 3 centigrade	light end	2	0.225	0.225	0.007089	1.595025	1105.000	17.7	20.80	8.019	1322.684	1307.741	602.027	7.827																																								27	7.76	20.59	591.68	593.68	1245.77	41.38	1348.65	0.68	1.12
laboratory	future pCO2	minus 3 centigrade	light end	3	0.200	0.250	0.007089	1.772250	1111.429	17.7	21.80	8.130	1297.771	1283.425	558.425	7.801																																								27	7.73	21.13	624.45	626.53	1222.81	39.49	1321.26	0.65	1.07
laboratory	CONTROL	control	dark start	1	0.225	0.225	0.007089	1.595025	147.143	17.7	23.30	7.688	1266.028	1252.515	601.035	7.933																																								27	7.86	14.75	453.94	455.43	1183.43	54.34	1318.75	0.91	1.49
laboratory	CONTROL	control	dark start	2	0.235	0.215	0.007089	1.524135	155.714	17.7	23.20	7.673	1264.260	1250.733	572.307	7.936																																								27	7.86	14.65	449.60	451.08	1181.63	54.46	1317.29	0.91	1.49
laboratory	CONTROL	control	dark start	3	0.195	0.255	0.007089	1.807695	164.286	17.7	23.20	7.698	1266.204	1252.657	564.774	8.014																																								27	7.94	12.18	373.84	375.06	1175.64	64.83	1336.68	1.08	1.77
laboratory	CONTROL	control	dark end	1	0.225	0.225	0.007089	1.595025	493.500	17.7	23.10	7.031	1298.173	1284.250	587.164	7.875																																								27	7.80	17.43	533.60	535.35	1218.26	48.56	1338.85	0.81	1.33
laboratory	CONTROL	control	dark end	2	0.235	0.215	0.007089	1.524135	500.000	17.7	23.10	6.998	1286.048	1272.254	557.567	7.934																																								27	7.86	15.02	459.80	461.31	1202.34	54.89	1338.66	0.91	1.50
laboratory	CONTROL	control	dark end	3	0.195	0.255	0.007089	1.807695	506.500	17.7	23.00	7.147	1292.224	1278.331	552.352	7.916																																								27	7.84	15.74	480.59	482.17	1209.66	52.93	1341.07	0.88	1.45
laboratory	CONTROL	control	light end	1	0.225	0.225	0.007089	1.595025	1079.286	17.7	23.30	7.620	1288.663	1274.909	596.675	7.972																																								27	7.90	13.70	421.77	423.14	1200.97	60.23	1350.27	1.01	1.65
laboratory	CONTROL	control	light end	2	0.235	0.215	0.007089	1.524135	1085.714	17.7	23.30	7.514	1285.733	1272.009	577.838	7.987																																								27	7.92	13.17	405.24	406.57	1196.61	62.24	1350.85	1.04	1.70
laboratory	CONTROL	control	light end	3	0.195	0.255	0.007089	1.807695	1092.143	17.7	23.40	7.800	1280.807	1267.170	568.936	8.094																																								27	8.02	10.13	312.55	313.57	1178.44	78.60	1372.62	1.31	2.15
laboratory	current pCO2	minus 3 centigrade															-0.8589	0.111	-0.7615	0.0981	0.6746	0.1378	0.5905	0.1206																	24.4	21.0	0.1	8.08	0.02	453.1	24.0	1750.8	2.9	123.90	6.4	2048.7	12.6	1889.8	3.3	27	8.00	14.95	447.94	449.45	1750.32	124.53	2049.73	2.02	3.21
laboratory	future pCO2	minus 3 centigrade															-0.6452	0.055	-0.7399	0.0628	0.7425	0.1389	0.8660	0.1620																	17.7	20.8	0.1	7.60	0.02	989.8	40.7	1232.9	2.6	24.28	0.9	1293.8	2.8	1291.6	2.9	27	7.53	34.39	988.54	991.88	1232.94	24.26	1293.76	0.40	0.66
laboratory	current pCO2	control															-1.0180	0.038	-0.9025	0.0339	0.7002	0.0117	0.6128	0.0102																	24.4	23.1	0.0	8.07	0.02	469.9	27.2	1744.7	4.1	130.20	6.9	2056.9	12.1	1889.6	2.0	27	7.98	14.81	469.75	471.29	1745.35	129.44	2055.87	2.13	3.36
laboratory	future pCO2	control															-0.8714	0.062	-0.9992	0.0713	0.9029	0.0412	1.0530	0.0480																	17.7	23.2	0.1	7.63	0.06	995.9	146.6	1281.6	5.5	29.50	4.3	1354.7	12.7	1343.4	6.1	27	7.56	32.17	987.53	990.76	1282.04	29.19	1354.34	0.49	0.80
laboratory	current pCO2	plus 3 centigrade															-1.1248	0.055	-0.9972	0.0486	-0.1820	0.2653	-0.1635	0.2281																	24.4	25.6	0.5	8.08	0.01	471.5	13.3	1723.5	2.0	136.90	3.4	2051.6	6.2	1874.5	1.5	27	7.99	13.82	467.64	469.13	1720.01	140.67	2056.86	2.35	3.69
laboratory	future pCO2	plus 3 centigrade															-0.8959	0.069	-1.0273	0.0785	0.4655	0.2199	0.5429	0.2564																	17.7	25.8	0.2	7.64	0.12	1011.1	248.6	1265.8	2.7	32.70	9.3	1346.7	23.6	1329.2	3.4	27	7.56	29.97	984.90	988.03	1267.22	32.01	1346.40	0.54	0.89
laboratory	current pCO2	plus 5 centigrade															-1.3158	0.085	-1.1664	0.0755	-0.4458	0.1108	-0.3901	0.0969																	24.4	27.1	0.1	8.11	0.05	445.2	56.2	1698.3	22.7	155.30	17.0	2069.3	17.6	1866.1	7.4	27	8.01	12.51	439.67	441.04	1698.11	155.48	2069.75	2.62	4.10
laboratory	future pCO2	plus 5 centigrade															-1.0244	0.063	-1.1747	0.0719	-0.5158	0.1491	-0.6016	0.1738																	17.7	27.9	0.1	7.65	0.12	989.6	40.7	1254.4	5.2	34.30	1.3	1339.2	3.8	1317.1	5.2	27	7.57	28.23	978.12	981.14	1254.31	34.56	1339.71	0.60	0.97
laboratory	current pCO2	CON															-1.0180	0.038	-0.9025	0.0339	0.7002	0.0117	0.6128	0.0102																	24.4	23.1	0.0	8.07	0.02	469.9	27.2	1744.7	4.1	130.20	6.9	2056.9	12.1	1889.6	2.0	27	7.98	14.81	469.75	471.29	1745.35	129.44	2055.87	2.13	3.36
laboratory	future pCO2	CON															-0.7871	0.016	-0.9025	0.0186	0.5255	0.0510	0.6128	0.0595																	17.7	23.3	0.1	7.96	0.05	431.9	45.7	1193.0	4.1	58.40	6.1	1338.2	10.9	1265.5	1.1	27	7.89	13.99	430.47	431.88	1193.25	58.26	1337.92	0.97	1.60
laboratory	current pCO2	minus 3 centigrade																							1.35195	0.18526	0.89	0.07	0.67654	0.22438	-0.55501	0.23563	320.93	64.79	-0.64	3.98	1.19	0.01	2.02	0.45	24.4	21.0	0.1	8.08	0.02	453.1	24.0	1750.8	2.9	123.90	6.4	2048.7	12.6	1889.8	3.3	27	8.00	14.95	447.94	449.45	1750.32	124.53	2049.73	2.02	3.21
laboratory	future pCO2	minus 3 centigrade																							1.60582	0.11128	1.09	0.16	0.83697	0.11745	-0.63529	0.38041	-1223.88	227.07	43.31	299.53	1.85	0.27	6.66	1.36	17.7	20.8	0.1	7.60	0.02	989.8	40.7	1232.9	2.6	24.28	0.9	1293.8	2.8	1291.6	2.9	27	7.53	34.39	988.54	991.88	1232.94	24.26	1293.76	0.40	0.66
laboratory	current pCO2	control																							1.51538	0.03702	0.84	0.01	1.19989	0.14943	-0.86079	0.21892	-570.91	86.07	610.01	60.25	1.85	0.16	4.00	0.27	24.4	23.1	0.0	8.07	0.02	469.9	27.2	1744.7	4.1	130.20	6.9	2056.9	12.1	1889.6	2.0	27	7.98	14.81	469.75	471.29	1745.35	129.44	2055.87	2.13	3.36
laboratory	future pCO2	control																							2.05223	0.10895	1.03	0.03	1.05609	0.16503	-1.24332	0.16203	-760.14	237.39	-148.24	537.23	2.42	1.01	6.11	1.39	17.7	23.2	0.1	7.63	0.06	995.9	146.6	1281.6	5.5	29.50	4.3	1354.7	12.7	1343.4	6.1	27	7.56	32.17	987.53	990.76	1282.04	29.19	1354.34	0.49	0.80
laboratory	current pCO2	plus 3 centigrade																							0.83369	0.18221	0.42	0.11	1.24265	0.16332	0.13195	0.07350	-480.34	381.25	189.38	238.78	1.88	0.42	4.47	2.10	24.4	25.6	0.5	8.08	0.01	471.5	13.3	1723.5	2.0	136.90	3.4	2051.6	6.2	1874.5	1.5	27	7.99	13.82	467.64	469.13	1720.01	140.67	2056.86	2.35	3.69
laboratory	future pCO2	plus 3 centigrade																							1.57014	0.26741	0.77	0.13	1.13509	0.18311	-0.61691	0.08793	-1182.04	183.18	-405.17	820.27	1.97	0.31	9.61	1.36	17.7	25.8	0.2	7.64	0.12	1011.1	248.6	1265.8	2.7	32.70	9.3	1346.7	23.6	1329.2	3.4	27	7.56	29.97	984.90	988.03	1267.22	32.01	1346.40	0.54	0.89
laboratory	current pCO2	plus 5 centigrade																							0.77629	0.17236	0.33	0.05	1.35529	0.17932	0.58527	0.36397	-719.96	380.98	-15.84	14.23	2.37	0.18	15.52	1.81	24.4	27.1	0.1	8.11	0.05	445.2	56.2	1698.3	22.7	155.30	17.0	2069.3	17.6	1866.1	7.4	27	8.01	12.51	439.67	441.04	1698.11	155.48	2069.75	2.62	4.10
laboratory	future pCO2	plus 5 centigrade																							0.57308	0.13381	0.25	0.07	1.35988	0.19320	1.42476	0.20241	-881.39	352.69	-782.00	340.26	2.40	0.58	14.68	4.42	17.7	27.9	0.1	7.65	0.12	989.6	40.7	1254.4	5.2	34.30	1.3	1339.2	3.8	1317.1	5.2	27	7.57	28.23	978.12	981.14	1254.31	34.56	1339.71	0.60	0.97