Citation:

PANGAEA.930756

Name: Seawater carbonate chemistry and gene expression levels of the limpet Nacella concinna
Published: 2021-04-26
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Antarctic; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; Mollusca; Nacella concinna; Polar; Single species

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook TwitterShow Map Google Earth

Abstract:

Ocean acidification (OA) could become a serious threat for the Antarctic marine ecosystem over coming years, as the solubility of atmospheric CO2 and CaCO3 minerals increases at lower temperatures. We evaluated the effect of OA on the stress response of the limpet Nacella concinna by measuring gene expression levels. The experiment was performed with the two ecotypes (Littoral and Sublittoral) of the species during 54 days (IPCC, 2019 scenario RCP8.5; control, 375 ppm; low-pH treatment, 923 ppm). Exposure to low-pH treatment during 15 days triggered the down-regulation of two heat-shock protein genes (HSP70A, HSP70B) only in sublittoral individuals. Little variation in the relative expression values of all genes in both ecotypes was observed probably, due to a historical exposure to the substantial daily natural pH fluctuations recorded in the study area during the experiment. This study provides relevant baseline data for future OA experiments on coastal species in Antarctica.

Keyword(s):

Animalia; Antarctic; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; Mollusca; Nacella concinna; Polar; Single species

Supplement to:

de Aranzamendi, M C; Servetto, Natalia; Movilla, Juancho; Bettencourt, Renato; Sahade, Ricardo José (2021): Ocean acidification effects on the stress response in a calcifying antarctic coastal organism: The case of Nacella concinna ecotypes. Marine Pollution Bulletin, 166, 112218, https://doi.org/10.1016/j.marpolbul.2021.112218

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -62.233300 * Longitude: -58.666600

Event(s):

Potter_Cove * Latitude: -62.233300 * Longitude: -58.666600 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-04-19.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		de Aranzamendi, M C		study
2	Species	Species		de Aranzamendi, M C
3	Registration number of species	Reg spec no		de Aranzamendi, M C
4	Uniform resource locator/link to reference	URL ref		de Aranzamendi, M C		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	de Aranzamendi, M C
6	Ecotype	Ecotype		de Aranzamendi, M C
7	Gene name	Gene		de Aranzamendi, M C
8	Experiment day	Exp day	day	de Aranzamendi, M C
9	Gene expression, fold change, relative	Gene expression		de Aranzamendi, M C
10	Gene expression, fold change, relative	Gene expression		de Aranzamendi, M C		min
11	Gene expression, fold change, relative	Gene expression		de Aranzamendi, M C		max
12	Comment	Comment		de Aranzamendi, M C		Gene regulation
13	Alkalinity, total	AT	µmol/kg	de Aranzamendi, M C	Potentiometric titration
14	Alkalinity, total, standard deviation	AT std dev	±	de Aranzamendi, M C	Potentiometric titration
15	pH	pH		de Aranzamendi, M C	Potentiometric	total scale
16	pH, standard deviation	pH std dev	±	de Aranzamendi, M C	Potentiometric	total scale
17	Temperature, water	Temp	°C	de Aranzamendi, M C
18	Temperature, water, standard deviation	Temp std dev	±	de Aranzamendi, M C
19	Salinity	Sal		de Aranzamendi, M C
20	Salinity, standard deviation	Sal std dev	±	de Aranzamendi, M C
21	Bicarbonate ion	[HCO3]-	µmol/kg	de Aranzamendi, M C	Calculated using CO2SYS
22	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	de Aranzamendi, M C	Calculated using CO2SYS
23	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	de Aranzamendi, M C	Calculated using CO2SYS
24	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	de Aranzamendi, M C	Calculated using CO2SYS
25	Dry air column-averaged mixing ratio of carbon dioxide	XCO2	ppmv	de Aranzamendi, M C	Calculated using CO2SYS
26	Dry air column-averaged mixing ratio of carbon dioxide, standard deviation	XCO2 std dev	±	de Aranzamendi, M C	Calculated using CO2SYS
27	Carbon dioxide	CO2	µmol/kg	de Aranzamendi, M C	Calculated using CO2SYS
28	Carbon dioxide, standard deviation	CO2 std dev	±	de Aranzamendi, M C	Calculated using CO2SYS
29	Calcite saturation state	Omega Cal		de Aranzamendi, M C	Calculated using CO2SYS
30	Calcite saturation state, standard deviation	Omega Cal std dev	±	de Aranzamendi, M C	Calculated using CO2SYS
31	Aragonite saturation state	Omega Arg		de Aranzamendi, M C	Calculated using CO2SYS
32	Aragonite saturation state, standard deviation	Omega Arg std dev	±	de Aranzamendi, M C	Calculated using CO2SYS
33	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
36	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
38	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
40	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
42	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
44	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
46	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
48	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1154 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Ecotype	7 Gene	8 Exp day [day]	9 Gene expression	10 Gene expression (min)	11 Gene expression (max)	12 Comment (Gene regulation)	13 AT [µmol/kg] (Potentiometric titration)	14 AT std dev [±] (Potentiometric titration)	15 pH (total scale, Potentiometric)	16 pH std dev [±] (total scale, Potentiometric)	17 Temp [°C]	18 Temp std dev [±]	19 Sal	20 Sal std dev [±]	21 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	22 [HCO3]- std dev [±] (Calculated using CO2SYS)	23 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	24 pCO2 std dev [±] (Calculated using CO2SYS)	25 XCO2 [ppmv] (Calculated using CO2SYS)	26 XCO2 std dev [±] (Calculated using CO2SYS)	27 CO2 [µmol/kg] (Calculated using CO2SYS)	28 CO2 std dev [±] (Calculated using CO2SYS)	29 Omega Cal (Calculated using CO2SYS)	30 Omega Cal std dev [±] (Calculated using CO2SYS)	31 Omega Arg (Calculated using CO2SYS)	32 Omega Arg std dev [±] (Calculated using CO2SYS)	33 CSC flag (Calculated using seacarb afte...)	34 CO2 [µmol/kg] (Calculated using seacarb afte...)	35 CO2 std dev [±] (Calculated using seacarb afte...)	36 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	37 fCO2 std dev [±] (Calculated using seacarb afte...)	38 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	39 pCO2 std dev [±] (Calculated using seacarb afte...)	40 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	41 [HCO3]- std dev [±] (Calculated using seacarb afte...)	42 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	43 [CO3]2- std dev [±] (Calculated using seacarb afte...)	44 DIC [µmol/kg] (Calculated using seacarb afte...)	45 DIC std dev [±] (Calculated using seacarb afte...)	46 Omega Arg (Calculated using seacarb afte...)	47 Omega Arg std dev [±] (Calculated using seacarb afte...)	48 Omega Cal (Calculated using seacarb afte...)	49 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	HSP70A	0	3.626	0.809	26.611		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	HSP70A	15	0.234	0.026	2.151		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	HSP70A	54	1.676	0.630	5.483		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	HSP70B	0	0.779	0.167	4.780		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	HSP70B	15	0.436	0.139	1.456		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	HSP70B	54	1.017	0.520	1.982		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	HSC70	0	1.476	0.478	5.973		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	HSC70	15	1.403	0.648	4.103		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	HSC70	54	0.555	0.277	1.211		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	Beta-Actin	0	2.212	0.258	9.755		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	Beta-Actin	15	1.951	0.487	10.028		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Littoral ecotype	Beta-Actin	54	0.662	0.195	1.833		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	HSP70A	0	2.669	0.094	103.169		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	HSP70A	15	0.023	0.004	0.211	Down	2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	HSP70A	54	2.822	0.813	11.229		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	HSP70B	0	1.342	0.132	13.192		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	HSP70B	15	0.196	0.048	0.721	Down	2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	HSP70B	54	0.798	0.252	2.155		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	HSC70	0	1.155	0.158	5.541		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	HSC70	15	1.054	0.435	2.627		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	HSC70	54	1.478	0.750	2.811		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	Beta-Actin	0	0.204	0.006	6.375		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	Beta-Actin	15	4.107	0.839	27.974		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24
laboratory	Nacella concinna (mollusk)	197296	marinespecies.org	58	Sublittoral ecotype	Beta-Actin	54	1.424	0.439	4.298		2450.6	31.7	7.721	0.088	0.87	0.23	33.53	0.18	2270.44	52.79	917.5	183.77	922.96	184.86	57.93	11.58	1.2	0.17	0.75	0.11	8	56.84	12.17	926.83	198.42	930.88	199.29	2325.85	38.86	50.11	9.81	2432.79	41.40	0.76	0.15	1.21	0.24