Citation:

PANGAEA.930632

Name: Seawater carbonate chemistry and pH of the extracellular calcifying fluid and skeletal concentrations of trace metals of three coral species (Pocillopora eydouxi, Porites lobata, and Porites rus)
Published: 2021-04-22
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Animalia; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Cnidaria; CO2 vent; Coast and continental shelf; Community composition and diversity; Entire community; Field observation; North Pacific; Pocillopora eydouxi; Porites lobata; Porites rus; Rocky-shore community; Single species; Tropical

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Abstract:

Ocean acidification (OA) threatens the growth and function of coral reef ecosystems. A key component to coral health is the microbiome, but little is known about the impact of OA on coral microbiomes. A submarine CO2 vent at Maug Island in the Northern Marianas Islands provides a natural pH gradient to investigate coral responses to long-term OA conditions. Three coral species (Pocillopora eydouxi, Porites lobata, and Porites rus) were sampled from three sites where mean seawater pH is 8.04, 7.98, and 7.94. We characterized coral bacterial communities (using 16S rRNA gene sequencing) and determined pH of the extracellular calcifying fluid (ECF) (using skeletal boron isotopes) across the seawater pH gradient. Bacterial communities of both Porites species stabilized (decreases in community dispersion) with decreased seawater pH, coupled with large increases in the abundance of Endozoicomonas, an endosymbiont. P. lobata experienced a significant decrease in ECF pH near the vent, whereas P. rus experienced a trending decrease in ECF pH near the vent. By contrast, Pocillopora exhibited bacterial community destabilization (increases in community dispersion), with significant decreases in Endozoicomonas abundance, while its ECF pH remained unchanged across the pH gradient. Our study shows that OA has multiple consequences on Endozoicomonas abundance and suggests that Endozoicomonas abundance may be an indicator of coral response to OA. We reveal an interesting dichotomy between two facets of coral physiology (regulation of bacterial communities and regulation of calcification), highlighting the importance of multidisciplinary approaches to understanding coral health and function in a changing ocean.

Keyword(s):

Acid-base regulation; Animalia; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Cnidaria; CO2 vent; Coast and continental shelf; Community composition and diversity; Entire community; Field observation; North Pacific; Pocillopora eydouxi; Porites lobata; Porites rus; Rocky-shore community; Single species; Tropical

Supplement to:

Shore, A; Day, R D; Stewart, J A; Burge, Colleen A (2021): Dichotomy between Regulation of Coral Bacterial Communities and Calcification Physiology under Ocean Acidification Conditions. Applied and Environmental Microbiology, 87(6), https://doi.org/10.1128/AEM.02189-20

Related to:

Enochs, I C; Manzello, Derek P; Donham, E M; Kolodziej, Graham; Okano, R; Johnston, Lyza; Young, Craig S; Iguel, John; Edwards, C B; Fox, M D; Valentino, L; Johnson, Steven; Benavente, D; Clark, S J; Carlton, R; Burton, T; Eynaud, Y; Price, Nichole N (2015): Shift from coral to macroalgae dominance on a volcanically acidified reef. PANGAEA, https://doi.org/10.1594/PANGAEA.867324

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 20.016600 * Longitude: 145.216600

Event(s):

Northern_Mariana_Islands * Latitude: 20.016600 * Longitude: 145.216600 * Method/Device: Experiment (EXP)

Comment:

For trace metal parameters, value 0 stands for value < limit of detection. In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-04-19.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Burge, Colleen A		study
2	Identification	ID		Burge, Colleen A
3	Species	Species		Burge, Colleen A
4	Registration number of species	Reg spec no		Burge, Colleen A
5	Uniform resource locator/link to reference	URL ref		Burge, Colleen A		WoRMS Aphia ID
6	Site	Site		Burge, Colleen A
7	δ11B	δ11B	‰ SRM	Burge, Colleen A
8	pH	pH		Burge, Colleen A		SeaFET
9	pH, standard error	pH std e	±	Burge, Colleen A		SeaFET
10	pH	pH		Burge, Colleen A		extracellular calcifying fluid
11	Calcium	Ca	mmol/l	Burge, Colleen A
12	Lithium/Calcium ratio	Li/Ca	µmol/mol	Burge, Colleen A		Li7(LR)/Ca46(LR)
13	Barium/Calcium ratio	Ba/Ca	µmol/mol	Burge, Colleen A		B11(LR)/Ca46(LR)
14	Aluminium/Calcium ratio	Al/Ca	µmol/mol	Burge, Colleen A		Al27(LR)/Ca46(LR)
15	Phosphorus/Calcium ratio	P/Ca	mmol/mol	Burge, Colleen A		P31(LR)/Ca46(LR)
16	Vanadium/Calcium ratio	V/Ca	µmol/mol	Burge, Colleen A		V51(LR)/Ca46(LR)
17	Chromium/Calcium ratio	Cr/Ca	µmol/mol	Burge, Colleen A		Cr52(LR)/Ca46(LR)
18	Manganese/Calcium ratio	Mn/Ca	µmol/mol	Burge, Colleen A		Mn55(LR)/Ca46(LR)
19	Cobalt/Calcium ratio	Co/Ca	µmol/mol	Burge, Colleen A		Co59(LR)/Ca46(LR)
20	Nickel/Calcium ratio	Ni/Ca	µmol/mol	Burge, Colleen A		Ni60(LR)/Ca46(LR)
21	Copper/Calcium ratio	Cu/Ca	µmol/mol	Burge, Colleen A		Cu63(LR)/Ca46(LR)
22	Zinc/Calcium ratio	Zn/Ca	µmol/mol	Burge, Colleen A		Zn66(LR)/Ca46(LR)
23	Rubidium/Calcium ratio	Rb/Ca	nmol/mol	Burge, Colleen A		Rb85(LR)/Ca46(LR)
24	Molybdenum/Calcium ratio	Mo/Ca	µmol/mol	Burge, Colleen A		Mo95(LR)/Ca46(LR)
25	Argentum/Calcium ratio	Ag/Ca	nmol/mol	Burge, Colleen A		Ag107(LR)/Ca46(LR)
26	Cadmium/Calcium ratio	Cd/Ca	µmol/mol	Burge, Colleen A		Cd111(LR)/Ca46(LR)
27	Tin/Calcium ratio	Sn/Ca	nmol/mol	Burge, Colleen A		Sn118(LR)/Ca46(LR)
28	Antimony/Calcium ratio	Sb/Ca	nmol/mol	Burge, Colleen A		Sb121(LR)/Ca46(LR)
29	Caesium/Calcium ratio	Cs/Ca	nmol/mol	Burge, Colleen A		Cs133(LR)/Ca46(LR)
30	Barium/Calcium ratio	Ba/Ca	µmol/mol	Burge, Colleen A		Ba138(LR)/Ca46(LR)
31	Neodymium/Calcium ratio	Nd/Ca	µmol/mol	Burge, Colleen A		Nd146(LR)/Ca46(LR)
32	Tungsten/Calcium ratio	W/Ca	nmol/mol	Burge, Colleen A		W182(LR)/Ca46(LR)
33	Lead/Calcium ratio	Pb/Ca	nmol/mol	Burge, Colleen A		Pb208(LR)/Ca46(LR)
34	Uranium/Calcium ratio	U/Ca	nmol/mol	Burge, Colleen A		U238(LR)/Ca46(LR)
35	Sodium/Calcium ratio	Na/Ca	mmol/mol	Burge, Colleen A		Na23(MR)/Ca48(MR)
36	Magnesium/Calcium ratio	Mg/Ca	mmol/mol	Burge, Colleen A		Mg25(MR)/Ca48(MR)
37	Magnesium/Calcium ratio	Mg/Ca	mmol/mol	Burge, Colleen A		Mg26(MR)/Ca48(MR)
38	Iron/Calcium ratio	Fe/Ca	µmol/mol	Burge, Colleen A		Fe56(MR)/Ca48(MR)
39	Strontium/Calcium ratio	Sr/Ca	mmol/mol	Burge, Colleen A		Sr86(MR)/Ca48(MR)
40	Strontium/Calcium ratio	Sr/Ca	mmol/mol	Burge, Colleen A		Sr87(MR)/Ca48(MR)
41	Lithium/Magnesium ratio	Li/Mg	µmol/mmol	Burge, Colleen A
42	Replicates	Repl	#	Burge, Colleen A
43	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Burge, Colleen A		site
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Burge, Colleen A		site
45	Number of sequences	Sequence no	#	Burge, Colleen A		Bacterial Sequences
46	Number of sequences, standard error	Sequence no std e	±	Burge, Colleen A		Bacterial Sequences
47	Species richness	S		Burge, Colleen A		Observed ASVs
48	Species richness, standard error	S std e	±	Burge, Colleen A		Observed ASVs
49	Shannon Diversity Index	H'		Burge, Colleen A
50	Shannon Diversity index, standard error	H' std e	±	Burge, Colleen A
51	Temperature, water	Temp	°C	Enochs, I C
52	Salinity	Sal		Enochs, I C
53	Alkalinity, total	AT	µmol/kg	Enochs, I C
54	Carbon, inorganic, dissolved	DIC	µmol/kg	Enochs, I C
55	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
57	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
59	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
61	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
63	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2779 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 ID	3 Species	4 Reg spec no	5 URL ref (WoRMS Aphia ID)	6 Site	7 δ11B [‰ SRM]	8 pH (SeaFET)	9 pH std e [±] (SeaFET)	10 pH (extracellular calcifying fluid)	11 Ca [mmol/l]	12 Li/Ca [µmol/mol] (Li7(LR)/Ca46(LR))	13 Ba/Ca [µmol/mol] (B11(LR)/Ca46(LR))	14 Al/Ca [µmol/mol] (Al27(LR)/Ca46(LR))	15 P/Ca [mmol/mol] (P31(LR)/Ca46(LR))	16 V/Ca [µmol/mol] (V51(LR)/Ca46(LR))	17 Cr/Ca [µmol/mol] (Cr52(LR)/Ca46(LR))	18 Mn/Ca [µmol/mol] (Mn55(LR)/Ca46(LR))	19 Co/Ca [µmol/mol] (Co59(LR)/Ca46(LR))	20 Ni/Ca [µmol/mol] (Ni60(LR)/Ca46(LR))	21 Cu/Ca [µmol/mol] (Cu63(LR)/Ca46(LR))	22 Zn/Ca [µmol/mol] (Zn66(LR)/Ca46(LR))	23 Rb/Ca [nmol/mol] (Rb85(LR)/Ca46(LR))	24 Mo/Ca [µmol/mol] (Mo95(LR)/Ca46(LR))	25 Ag/Ca [nmol/mol] (Ag107(LR)/Ca46(LR))	26 Cd/Ca [µmol/mol] (Cd111(LR)/Ca46(LR))	27 Sn/Ca [nmol/mol] (Sn118(LR)/Ca46(LR))	28 Sb/Ca [nmol/mol] (Sb121(LR)/Ca46(LR))	29 Cs/Ca [nmol/mol] (Cs133(LR)/Ca46(LR))	30 Ba/Ca [µmol/mol] (Ba138(LR)/Ca46(LR))	31 Nd/Ca [µmol/mol] (Nd146(LR)/Ca46(LR))	32 W/Ca [nmol/mol] (W182(LR)/Ca46(LR))	33 Pb/Ca [nmol/mol] (Pb208(LR)/Ca46(LR))	34 U/Ca [nmol/mol] (U238(LR)/Ca46(LR))	35 Na/Ca [mmol/mol] (Na23(MR)/Ca48(MR))	36 Mg/Ca [mmol/mol] (Mg25(MR)/Ca48(MR))	37 Mg/Ca [mmol/mol] (Mg26(MR)/Ca48(MR))	38 Fe/Ca [µmol/mol] (Fe56(MR)/Ca48(MR))	39 Sr/Ca [mmol/mol] (Sr86(MR)/Ca48(MR))	40 Sr/Ca [mmol/mol] (Sr87(MR)/Ca48(MR))	41 Li/Mg [µmol/mmol]	42 Repl [#]	43 pCO2water_SST_wet [µatm] (site)	44 pCO2water_SST_wet std e [±] (site)	45 Sequence no [#] (Bacterial Sequences)	46 Sequence no std e [±] (Bacterial Sequences)	47 S (Observed ASVs)	48 S std e [±] (Observed ASVs)	49 H'	50 H' std e [±]	51 Temp [°C]	52 Sal	53 AT [µmol/kg]	54 DIC [µmol/kg]	55 CSC flag	56 pH (total scale)	57 CO2 [µmol/kg]	58 fCO2water_SST_wet [µatm]	59 pCO2water_SST_wet [µatm]	60 [HCO3]- [µmol/kg]	61 [CO3]2- [µmol/kg]	62 Omega Arg	63 Omega Cal
field	BPoc1	Pocillopora eydouxi (coral)	206958	marinespecies.org	Background pH	24.32	8.04	0.016	8.51	2.021	5.983	572.366	12.205	0.189	73750.120	0	0.487	0.464	6.107	0.169	5.503	10.490	0.011794	0.000	0.022	107.892	9.327	0	4.667	0.003	0	30.164	1668.037	20.985	3.872	3.867	23.217	9.515	9.548	1.545		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPoc2	Pocillopora eydouxi (coral)	206958	marinespecies.org	Background pH	24.74	8.04	0.016	8.54	2.086	6.014	577.333	15.105	0.322	80388.815	0	0.955	0.494	6.585	0.489	9.470	9.294	0.011072	0.000	0.041	95.085	8.831	0	4.367	0.002	0	29.170	1366.362	21.148	4.261	4.222	233.852	9.319	9.335	1.411		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPoc3	Pocillopora eydouxi (coral)	206958	marinespecies.org	Background pH	23.78	8.04	0.016	8.48	2.047	6.080	577.530	14.432	0.237	68990.808	0	0.490	0.462	6.172	0.215	7.444	9.019	0.010158	0.000	0.029	76.302	7.699	0	4.431	0.001	0	25.387	1281.525	21.481	4.245	4.272	5.048	9.293	9.361	1.432		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPoc4	Pocillopora eydouxi (coral)	206958	marinespecies.org	Background pH	24.14	8.04	0.016	8.50	2.113	6.418	588.531	12.870	0.320	72815.567	0	0.606	0.463	6.113	0.343	9.198	9.359	0.010727	0.000	0.062	80.320	10.111	0	4.518	0.005	0	36.262	1518.377	21.870	4.292	4.256	24.161	9.443	9.473	1.495		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPoc5	Pocillopora eydouxi (coral)	206958	marinespecies.org	Background pH	24.37	8.04	0.016	8.52	1.881	6.368	615.764	9.103	0.318	73551.605	0	0.609	0.473	6.228	0.364	9.342	10.199	0.011547	0.000	0.079	76.885	10.174	0	4.536	0.003	0	28.363	1624.804	21.766	4.195	4.139	61.815	9.515	9.531	1.518		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	MPoc2	Pocillopora eydouxi (coral)	206958	marinespecies.org	Mid pH	24.49	7.98	0.027	8.53	2.052	6.596	616.974	8.615	0.190	66984.187	0	5.311	0.471	6.066	0.186	8.324	11.275	0.010347	0.000	0.014	111.391	7.955	0	4.718	0.001	0	14.608	1508.168	22.077	4.199	4.097	16.143	9.514	9.491	1.571		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPoc3	Pocillopora eydouxi (coral)	206958	marinespecies.org	Mid pH	25.37	7.98	0.027	8.58	2.068	6.164	595.399	15.643	0.247	69353.162	0	4.821	0.478	6.204	0.374	8.426	9.617	0.011699	0.000	0.020	75.185	10.101	0	4.980	0.005	0	29.828	1923.796	20.011	3.485	3.479	33.958	9.794	9.825	1.769		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPoc4	Pocillopora eydouxi (coral)	206958	marinespecies.org	Mid pH	24.21	7.98	0.027	8.51	2.101	6.264	604.367	11.650	0.239	67844.754	0	6.449	0.466	5.997	0.525	10.198	9.468	0.009790	0.000	0.023	101.424	8.360	0	4.766	0.002	0	16.904	1501.250	22.013	4.202	4.141	19.166	9.495	9.478	1.491		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPoc5	Pocillopora eydouxi (coral)	206958	marinespecies.org	Mid pH	23.62	7.98	0.027	8.47	1.911	6.731	586.235	14.471	0.213	68005.049	0	7.755	0.474	6.039	0.400	7.640	9.708	0.010043	0.000	0.020	85.609	8.082	0	4.777	0.001	0	55.307	1248.667	22.494	4.616	4.550	14.532	9.279	9.275	1.458		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPoc6	Pocillopora eydouxi (coral)	206958	marinespecies.org	Mid pH	23.07	7.98	0.027	8.43	2.047	6.993	545.588	10.301	0.241	69683.632	0	8.465	0.474	6.108	0.462	8.670	21.294	0.009965	0.000	0.019	107.399	7.003	0	13.756	0.001	0	14.024	1183.816	24.126	5.193	5.223	50.547	9.224	9.264	1.347		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	LPoc1	Pocillopora eydouxi (coral)	206958	marinespecies.org	Low pH	23.78	7.94	0.051	8.48	2.062	6.431	591.820	13.843	0.220	65284.358	0	11.107	0.469	6.091	0.242	6.283	10.060	0.009300	0.000	0.016	69.901	7.855	0	4.976	0.001	0	9.732	1420.356	21.911	4.411	4.456	26.520	9.406	9.469	1.458		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPoc2	Pocillopora eydouxi (coral)	206958	marinespecies.org	Low pH	24.24	7.94	0.051	8.51	2.006	7.126	546.839	12.612	0.277	62012.928	0	11.241	0.470	6.035	0.697	10.240	9.970	0.010356	0.000	0.028	93.688	8.733	0	4.971	0.001	0	14.200	1495.722	20.666	5.444	5.470	25.157	9.415	9.506	1.309		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPoc3	Pocillopora eydouxi (coral)	206958	marinespecies.org	Low pH	22.65	7.94	0.051	8.41	2.072	7.092	573.069	13.109	0.271	79598.623	0	13.229	0.486	6.211	0.284	9.134	61.356	0.010263	0.000	0.025	111.590	6.946	0	6.144	0.001	0	10.096	1242.510	29.012	5.731	5.752	86.565	9.286	9.343	1.237		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPoc4	Pocillopora eydouxi (coral)	206958	marinespecies.org	Low pH	25.25	7.94	0.051	8.57	1.985	6.238	602.612	15.480	0.214	63313.469	0	10.636	0.469	6.773	0.276	6.827	10.568	0.010883	0.000	0.017	125.435	7.685	0	5.524	0.001	0	13.032	1407.276	20.801	4.496	4.504	20.224	9.382	9.462	1.387		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPoc5	Pocillopora eydouxi (coral)	206958	marinespecies.org	Low pH	24.26	7.94	0.051	8.51	2.024	6.184	584.437	13.691	0.220	63084.135	0	10.460	0.472	6.092	0.432	13.530	11.224	0.009839	0.000	0.019	148.553	10.895	0	4.767	0.001	0	29.700	1350.336	21.567	4.156	4.175	17.579	9.284	9.346	1.488		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	BPor1	Porites lobata (coral)	207225	marinespecies.org	Background pH	25.42	8.04	0.016	8.58	2.231	6.014	556.143	131.375	0.186	72285.178	0	0.582	0.420	5.550	1.313	11.942	8.993	0.008419	0.000	0.020	70.842	7.831	0	3.945	0.002	0	15.835	1004.694	21.090	4.789	4.747	7.533	8.882	8.869	1.256		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPor2	Porites lobata (coral)	207225	marinespecies.org	Background pH	24.37	8.04	0.016	8.52	1.988	6.081	545.252	15.920	0.303	75515.340	0	0.589	0.430	5.769	0.381	19.886	8.913	0.009051	0.000	0.027	70.495	7.912	0	4.173	0.003	0	22.334	1079.430	21.474	4.701	4.684	11.883	8.919	8.953	1.294		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPor3	Porites lobata (coral)	207225	marinespecies.org	Background pH	25.10	8.04	0.016	8.56	2.185	6.173	542.429	15.240	0.289	69359.443	0	0.664	0.439	5.840	0.456	9.950	9.203	0.007090	0.000	0.024	65.112	6.823	0	4.118	0.001	0	47.379	962.530	21.768	4.872	4.860	6.201	8.852	8.875	1.267		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPor4	Porites lobata (coral)	207225	marinespecies.org	Background pH	24.70	8.04	0.016	8.54	2.072	6.039	510.774	7.908	0.409	68700.424	0	0.541	0.430	5.792	0.346	15.544	9.254	0.007654	0.000	0.017	90.149	7.398	0	3.953	0.000	0	16.989	928.502	21.240	4.657	4.600	6.201	8.777	8.798	1.297		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPor5	Porites lobata (coral)	207225	marinespecies.org	Background pH	25.22	8.04	0.016	8.57	2.042	6.008	549.107	2.914	0.247	67760.823	0	0.497	0.436	5.872	0.214	9.273	9.165	0.008259	0.000	0.025	115.665	9.014	0	3.915	0.001	0	11.448	979.508	21.239	4.521	4.553	3.804	8.803	8.854	1.329		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	MPor1	Porites lobata (coral)	207225	marinespecies.org	Mid pH	25.64	7.98	0.027	8.60	2.031	5.944	550.050	49.308	0.200	82514.861	0	3.515	0.433	5.693	0.255	11.805	9.061	0.007548	0.000	0.016	120.078	6.918	0	377.226	0.006	0	16.777	1044.168	20.610	4.589	4.628	16.397	9.210	9.292	1.295		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPor2	Porites lobata (coral)	207225	marinespecies.org	Mid pH	25.62	7.98	0.027	8.60	2.159	5.785	531.855	28.770	0.382	68231.239	0	3.069	0.436	5.866	0.317	14.657	8.947	0.008397	0.000	0.034	141.630	7.366	0	4.771	0.002	0	20.305	1196.186	20.019	4.002	4.028	16.800	8.993	9.043	1.446		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPor3	Porites lobata (coral)	207225	marinespecies.org	Mid pH	26.02	7.98	0.027	8.62	2.046	6.135	547.168	38.312	0.318	93485.002	0	7.026	0.434	5.800	0.472	15.556	11.098	0.008573	0.000	0.024	127.934	6.722	0	4.264	0.003	0	14.784	1055.178	21.199	4.978	4.949	55.515	8.921	8.972	1.232		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPor4	Porites lobata (coral)	207225	marinespecies.org	Mid pH	24.61	7.98	0.027	8.53	2.037	6.041	450.771	32.188	0.293	104006.759	0	7.474	0.431	5.713	0.370	16.473	8.504	0.007809	0.000	0.010	102.525	6.229	0	415.252	0.006	0	14.437	1014.052	20.472	5.081	5.070	38.493	9.194	9.252	1.189		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPor5	Porites lobata (coral)	207225	marinespecies.org	Mid pH	24.44	7.98	0.027	8.52	2.888	6.354	453.988	8.996	0.242	85338.298	0	4.304	0.423	5.926	0.289	11.235	8.510	0.008109	0.000	0.018	111.126	7.050	0	465.911	0.005	0	12.790	1053.895	20.414	5.501	5.507	14.638	9.141	9.204	1.155		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	LPor2	Porites lobata (coral)	207225	marinespecies.org	Low pH	22.91	7.94	0.051	8.42	2.054	7.036	350.572	21.950	0.335	131983.191	0	21.316	0.432	5.490	0.530	27.501	7.765	0.007368	0.000	0.016	115.191	6.217	0	1272.280	0.008	0	23.441	1036.952	19.185	7.448	7.385	91.748	9.616	9.610	0.945		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPor3	Porites lobata (coral)	207225	marinespecies.org	Low pH	23.62	7.94	0.051	8.47	2.165	6.338	484.341	21.594	0.372	90089.447	0	13.925	0.428	5.558	0.367	17.774	8.003	0.008405	0.000	0.011	126.461	7.366	0	1070.008	0.006	0	15.565	1117.994	20.327	5.113	5.079	40.922	9.282	9.321	1.239		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPor4	Porites lobata (coral)	207225	marinespecies.org	Low pH	24.36	7.94	0.051	8.52	2.231	6.169	425.721	21.639	0.223	87941.792	0	17.537	0.446	5.890	0.456	21.836	8.681	0.007234	0.000	0.009	179.103	6.316	0	6.944	0.004	0	46.459	1072.308	19.544	5.312	5.291	63.991	8.983	9.008	1.161		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPor5	Porites lobata (coral)	207225	marinespecies.org	Low pH	23.50	7.94	0.051	8.46	2.117	6.211	377.819	16.909	0.169	98346.377	0	14.179	0.455	6.067	0.463	20.062	0.000	0.008630	0.000	0.006	260.776	6.045	0	5.791	0.006	0	13.318	1168.204	18.565	5.490	5.414	54.905	9.006	9.025	1.131		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPor6	Porites lobata (coral)	207225	marinespecies.org	Low pH	24.47	7.94	0.051	8.52	2.071	6.476	460.003	7.355	0.409	77480.779	0	9.501	0.435	5.651	0.354	28.123	8.925	0.009885	0.000	0.014	226.676	9.492	0	1017.881	0.006	0	15.453	1118.073	20.761	5.201	5.129	40.729	9.275	9.254	1.245		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	BPrus1	Porites rus (coral)	1462269	marinespecies.org	Background pH	25.86	8.04	0.016	8.61	2.140	5.899	517.126	6.885	0.000	75689.270	0	0.417	0.467	6.048	0.104	4.286	0.000	0.005965	0.000	0.016	104.411	6.907	0	165.316	0.001	0	24.174	1226.850	20.511	3.702	3.554	5.495	9.776	9.592	1.593		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPrus2	Porites rus (coral)	1462269	marinespecies.org	Background pH	25.62	8.04	0.016	8.60	1.972	5.846	529.104	0.000	0.000	91613.717	0	0.380	0.466	5.978	0.123	6.389	0.000	0.005561	0.000	0.062	181.663	6.932	0	154.250	0.002	0	16.224	1297.714	20.335	3.563	3.510	4.087	9.844	9.823	1.641		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPrus3	Porites rus (coral)	1462269	marinespecies.org	Background pH	25.81	8.04	0.016	8.61	2.071	6.027	516.652	0.000	0.000	69620.773	0	0.535	0.472	6.039	0.143	7.621	0.000	0.004673	0.000	0.035	110.861	6.322	0	104.737	0.003	0	19.358	1318.599	20.595	3.942	3.839	3.732	9.588	9.518	1.529		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPrus4	Porites rus (coral)	1462269	marinespecies.org	Background pH	26.13	8.04	0.016	8.63	2.004	5.856	549.718	1.938	0.000	77281.988	0	0.596	0.466	6.035	0.150	5.321	0.000	0.006175	0.000	0.041	138.179	6.500	0	134.463	0.001	0	32.825	1345.504	20.560	3.700	3.639	4.675	10.061	10.009	1.583		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	BPrus5	Porites rus (coral)	1462269	marinespecies.org	Background pH	26.36	8.04	0.016	8.64	1.989	5.869	544.609	0.000	0.000	76421.452	0	0.428	0.473	6.042	0.062	5.720	0.000	0.006611	0.000	0.017	145.343	5.919	0	113.323	0.005	0	16.987	1379.969	20.620	3.741	3.588	2.748	9.919	9.750	1.569		401.02	4.61							29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field	MPrus1	Porites rus (coral)	1462269	marinespecies.org	Mid pH	25.37	7.98	0.027	8.58	2.149	6.209	484.510	2.351	0.000	93223.651	0	1.225	0.471	5.977	0.261	7.329	0.000	0.005779	5.178	0.016	267.180	5.730	0	185.392	0.006	0	14.884	1357.700	19.939	4.752	4.578	8.402	9.975	9.814	1.307		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPrus2	Porites rus (coral)	1462269	marinespecies.org	Mid pH	24.45	7.98	0.027	8.52	1.951	6.380	492.087	12.195	0.000	74492.018	0	1.352	0.481	6.022	0.102	5.779	0.000	0.005342	0.000	0.021	120.518	5.149	0	93.751	0.003	0	13.037	1191.835	21.702	4.433	4.366	5.167	9.781	9.784	1.439		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPrus3	Porites rus (coral)	1462269	marinespecies.org	Mid pH	25.58	7.98	0.027	8.60	2.126	5.974	488.450	18.946	0.000	81727.203	0	1.487	0.475	5.980	0.210	6.146	0.000	0.006114	0.000	0.022	114.118	5.705	0	217.223	0.006	0	15.222	1399.959	20.139	4.143	4.074	16.504	10.105	10.102	1.442		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPrus4	Porites rus (coral)	1462269	marinespecies.org	Mid pH	25.05	7.98	0.027	8.56	1.896	5.925	491.486	0.000	0.000	73888.367	0	0.985	0.483	6.148	0.109	5.182	0.000	0.005443	0.000	0.024	124.563	9.452	0	106.921	0.003	0	16.733	1327.355	20.392	3.833	3.795	4.130	9.837	9.874	1.546		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	MPrus5	Porites rus (coral)	1462269	marinespecies.org	Mid pH	26.23	7.98	0.027	8.64	2.126	5.874	485.743	0.000	0.000	85551.806	0	1.267	0.475	6.039	0.154	7.292	0.000	0.005884	0.000	0.023	157.735	6.308	0	206.886	0.004	0	21.646	1354.698	19.699	4.119	4.136	8.452	9.933	9.984	1.426		441.20	21.23							29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field	LPrus1	Porites rus (coral)	1462269	marinespecies.org	Low pH	25.51	7.94	0.051	8.59	2.261	5.951	493.316	5.109	0.000	69021.474	0	1.562	0.468	5.888	0.135	10.663	0.000	0.006137	0.000	0.015	120.011	5.773	0	224.448	0.003	0	18.934	1291.194	20.123	4.117	4.131	6.304	9.855	9.931	1.445		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPrus2	Porites rus (coral)	1462269	marinespecies.org	Low pH	24.20	7.94	0.051	8.51	2.029	6.334	499.955	0.000	0.000	67723.152	0	1.458	0.474	5.931	0.107	124.999	0.000	0.005197	0.000	0.021	140.401	5.230	0	187.142	0.001	0	16.429	1195.524	21.311	4.238	4.232	5.048	10.175	10.241	1.494		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPrus3	Porites rus (coral)	1462269	marinespecies.org	Low pH	23.14	7.94	0.051	8.44	2.082	7.458	340.893	0.000	0.000	82316.122	0	1.686	0.476	5.936	0.080	10.130	0.000	0.005420	0.000	0.016	155.522	5.233	0	97.594	0.010	0	18.339	1434.024	17.455	8.263	8.282	8.466	9.678	9.665	0.903		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPrus4	Porites rus (coral)	1462269	marinespecies.org	Low pH	26.20	7.94	0.051	8.63	2.111	5.540	490.027	3.546	0.000	71100.253	0	1.823	0.486	6.216	0.208	8.100	0.000	0.005885	0.000	0.024	132.437	6.951	0	62.135	0.004	0	18.407	1356.843	19.509	3.671	3.675	15.566	9.748	9.759	1.509		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field	LPrus5	Porites rus (coral)	1462269	marinespecies.org	Low pH		7.94	0.051		1.792	5.690	469.695	5.005	0.000	69269.728	0	1.005	0.488	6.232	0.175	6.158	0.000	0.005798	0.000	0.010	183.237	6.015	0	146.811	0.008	0	20.907	1359.757	19.116	3.704	3.680	8.218	9.760	9.771	1.536		502.00	29.67							29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field		Pocillopora eydouxi (coral)	206958	marinespecies.org	Background pH		8.04	0.016																																	10	401.02	4.61	42521	4251	31	3	3.71	0.19	29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field		Pocillopora eydouxi (coral)	206958	marinespecies.org	Mid pH		7.98	0.027																																	9	441.20	21.23	28556	5298	27	3	3.65	0.22	29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field		Pocillopora eydouxi (coral)	206958	marinespecies.org	Low pH		7.94	0.051																																	8	502.00	29.67	9205	3168	21	7	3.11	0.43	29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field		Porites lobata (coral)	207225	marinespecies.org	Background pH		8.04	0.016																																	7	401.02	4.61	4202	1105	71	12	5.35	0.19	29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field		Porites lobata (coral)	207225	marinespecies.org	Mid pH		7.98	0.027																																	10	441.20	21.23	13154	2677	245	33	7.02	0.28	29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field		Porites lobata (coral)	207225	marinespecies.org	Low pH		7.94	0.051																																	10	502.00	29.67	3968	598	35	15	3.50	0.48	29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09
field		Porites rus (coral)	1462269	marinespecies.org	Background pH		8.04	0.016																																	9	401.02	4.61	6036	1593	33	9	3.65	0.25	29.96	34.83	2279.41	1944.82	15	8.03	10.11	400.78	401.99	1698.04	236.68	3.86	5.76
field		Porites rus (coral)	1462269	marinespecies.org	Mid pH		7.98	0.027																																	9	441.20	21.23	5610	1078	26	3	3.55	0.14	29.96	34.82	2282.39	1970.30	15	8.00	11.34	449.56	450.92	1736.55	222.41	3.63	5.42
field		Porites rus (coral)	1462269	marinespecies.org	Low pH		7.94	0.051																																	10	502.00	29.67	4487	1468	19	4	3.02	0.22	29.98	34.82	2283.43	1992.73	15	7.96	12.64	501.30	502.81	1771.26	208.83	3.40	5.09