Citation:

PANGAEA.930496

Name: Seawater carbonate chemistry and carbamazepine bioaccumulation of Mytilus galloprovincialis
Published: 2021-04-20
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Immunology/Self-protection; Laboratory experiment; Mediterranean Sea; Mollusca; Mytilus galloprovincialis; Organic toxins; Other metabolic rates; Other studied parameter or process; Single species; Temperate

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

Contaminants of emerging concern and ocean changes are key environmental stressors for marine species with possibly synergistic, but still unexplored, deleterious effects. In the present study the influence of a simulated ocean acidification scenario (pH = 7.6) was investigated on metabolism and sub-lethal effects of carbamazepine, CBZ (1 µg/L), chosen as one of the most widely diffused pharmaceuticals in marine organisms. A multidisciplinary approach was applied on mussels, M. galloprovincialis, integrating measurement of drug bioaccumulation with changes in the whole transcriptome, responsiveness of various biochemical and cellular biomarkers including immunological parameters, lipid and oxidative metabolism, onset of genotoxic effects. Chemical analyses revealed a limited influence of hypercapnia on accumulation and excretion of CBZ, while a complex network of biological responses was observed in gene expression profile and functional changes at cellular level. The modulation of gamma-aminobutyric acid (GABA) pathway suggested similarities with the Mechanism of Action known for vertebrates: immune responses, cellular homeostasis and oxidative system represented the processes targeted by combined stressors. The overall elaboration of results through a quantitative Weight of Evidence model, revealed clearly increased cellular hazard due to interactions of CBZ with acidification compared to single stressors.

Keyword(s):

Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Immunology/Self-protection; Laboratory experiment; Mediterranean Sea; Mollusca; Mytilus galloprovincialis; Organic toxins; Other metabolic rates; Other studied parameter or process; Single species; Temperate

Supplement to:

Mezzelani, Marica; Nardi, Alessandro; Bernardini, Ilaria; Milan, Massimo; Peruzza, Luca; d'Errico, Giuseppe; Fattorini, Daniele; Gorbi, Stefania; Patarnello, Tomaso; Regoli, Francesco (2021): Environmental pharmaceuticals and climate change: The case study of carbamazepine in M. galloprovincialis under ocean acidification scenario. Environment International, 146, 106269, https://doi.org/10.1016/j.envint.2020.106269

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-04-19.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Regoli, Francesco		study
2	Species	Species		Regoli, Francesco
3	Registration number of species	Reg spec no		Regoli, Francesco
4	Uniform resource locator/link to reference	URL ref		Regoli, Francesco		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Regoli, Francesco
6	Treatment	Treat		Regoli, Francesco
7	Sample code/label	Sample label		Regoli, Francesco
8	Salinity	Sal		Regoli, Francesco
9	Salinity, standard deviation	Sal std dev	±	Regoli, Francesco
10	Temperature, water	Temp	°C	Regoli, Francesco
11	Temperature, water, standard deviation	Temp std dev	±	Regoli, Francesco
12	pH	pH		Regoli, Francesco	Potentiometric	NBS scale
13	pH, standard deviation	pH std dev	±	Regoli, Francesco	Potentiometric	NBS scale
14	Alkalinity, total	AT	µmol/kg	Regoli, Francesco	Potentiometric titration
15	Alkalinity, total, standard deviation	AT std dev	±	Regoli, Francesco	Potentiometric titration
16	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Regoli, Francesco	Calculated using CO2SYS
17	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Regoli, Francesco	Calculated using CO2SYS
18	Calcite saturation state	Omega Cal		Regoli, Francesco	Calculated using CO2SYS
19	Calcite saturation state, standard deviation	Omega Cal std dev	±	Regoli, Francesco	Calculated using CO2SYS
20	Aragonite saturation state	Omega Arg		Regoli, Francesco	Calculated using CO2SYS
21	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Regoli, Francesco	Calculated using CO2SYS
22	Carbamazepine, per dry mass	CBZ/dm	ng/g	Regoli, Francesco		d0
23	Carbamazepine, standard deviation	CBZ std dev	±	Regoli, Francesco		d0
24	Carbamazepine, per dry mass	CBZ/dm	ng/g	Regoli, Francesco		d3
25	Carbamazepine, standard deviation	CBZ std dev	±	Regoli, Francesco		d3
26	Carbamazepine, per dry mass	CBZ/dm	ng/g	Regoli, Francesco		d7
27	Carbamazepine, standard deviation	CBZ std dev	±	Regoli, Francesco		d7
28	Carbamazepine, per dry mass	CBZ/dm	ng/g	Regoli, Francesco		d14
29	Carbamazepine, standard deviation	CBZ std dev	±	Regoli, Francesco		d14
30	Carbamazepine, per dry mass	CBZ/dm	ng/g	Regoli, Francesco		d21
31	Carbamazepine, standard deviation	CBZ std dev	±	Regoli, Francesco		d21
32	Carbamazepine, per dry mass	CBZ/dm	ng/g	Regoli, Francesco		d28
33	Carbamazepine, standard deviation	CBZ std dev	±	Regoli, Francesco		d28
34	Carbamazepine, per dry mass	CBZ/dm	ng/g	Regoli, Francesco		d31
35	Carbamazepine, standard deviation	CBZ std dev	±	Regoli, Francesco		d31
36	Carbamazepine, per dry mass	CBZ/dm	ng/g	Regoli, Francesco		d38
37	Carbamazepine, standard deviation	CBZ std dev	±	Regoli, Francesco		d38
38	Hemocytes lysosomal membranes stability	LMS	min	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
39	Hemocytes lysosomal membranes stability, standard error	LMS std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
40	Granulocytes/Hyalinocytes ratio	G/H		Regoli, Francesco		measured at day 28, end of carbamazepine exposure
41	Granulocytes/Hyalinocytes ratio, standard error	G/H std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
42	Phagocytosis	Phago	%	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
43	Phagocytosis, standard error	Phago std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
44	Acetylcholinesterase activity, per protein mass	AChE/prot	nmol/min/mg	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
45	Acetylcholinesterase activity, per protein mass, standard error	AChE/prot std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
46	Hemocytes micronuclei frequency	Hem MN	‰	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
47	Hemocytes micronuclei frequency, standard error	MN std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
48	DNA fragmentation	DNA fragm	%	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
49	DNA fragmentation, standard error	DNA fragm std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
50	Lipofuscin	Lipo	intensity/µm²	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
51	Lipofuscin, standard error	Lipo std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
52	Neutral lipids	NL	intensity/µm²	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
53	Neutral lipids, standard error	NL std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
54	Malondialdehyde, per wet mass	MDA	nmol/g	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
55	Malondialdehyde, per wet mass, standard error	MDA std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
56	Acyl-CoA oxidase activity, per protein mass	ACOX/prot	nmol/min/mg	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
57	Acyl-CoA oxidase activity, per protein mass, standard deviation	ACOX/prot std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
58	Catalase activity, per protein mass	CAT/prot	µmol/min/mg	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
59	Catalase activity, per protein mass, standard error	CAT/prot std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
60	Glutathione S-transferase, activity per protein mass	GST/prot	nmol/min/mg	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
61	Glutathione S-transferase, activity per protein mass, standard error	GST std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
62	Glutathione reductase, per protein mass	GR/prot	nmol/min/mg	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
63	Glutathione reductase, per protein mass, standard error	GR std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
64	Glutathione, total, per unit wet mass	TGSH	µmol/g	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
65	Glutathione, total, per unit wet mass, standard error	TGSH std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
66	Selenium dependent glutathione peroxidases activity, per protein mass	GPxSe-Dep/prot	nmol/min/mg	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
67	Selenium dependent glutathione peroxidases activity, per protein mass, standard error	GPxSe-Dep/prot std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
68	Total glutathione peroxidases activity, per protein mass	GPxTOT/prot	nmol/min/mg	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
69	Total glutathione peroxidases activity, unit per protein mass, standard error	GPxTOT std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
70	Total oxyradical scavenging capacity against peroxyl radicals, unit per protein mass	TOSC-ROO	U/mg	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
71	Total oxyradical scavenging capacity against peroxyl radicals, unit per protein mass, standard error	TOSC-ROO std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
72	Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein mass	TOSC-HO	U/mg	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
73	Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein mass, standard error	TOSC-HO std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
74	Total oxyradical scavenging capacity against peroxynitrite, unit per protein mass	TOSC-ONOOH	U/mg	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
75	Total oxyradical scavenging capacity against peroxynitrite, unit per protein mass, standard error	TOSC-ONOOH std e	±	Regoli, Francesco		measured at day 28, end of carbamazepine exposure
76	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
77	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
78	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
79	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
80	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
81	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
82	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
83	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
84	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
85	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

331 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Treat	7 Sample label	8 Sal	9 Sal std dev [±]	10 Temp [°C]	11 Temp std dev [±]	12 pH (NBS scale, Potentiometric)	13 pH std dev [±] (NBS scale, Potentiometric)	14 AT [µmol/kg] (Potentiometric titration)	15 AT std dev [±] (Potentiometric titration)	16 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	17 pCO2 std dev [±] (Calculated using CO2SYS)	18 Omega Cal (Calculated using CO2SYS)	19 Omega Cal std dev [±] (Calculated using CO2SYS)	20 Omega Arg (Calculated using CO2SYS)	21 Omega Arg std dev [±] (Calculated using CO2SYS)	22 CBZ/dm [ng/g] (d0)	23 CBZ std dev [±] (d0)	24 CBZ/dm [ng/g] (d3)	25 CBZ std dev [±] (d3)	26 CBZ/dm [ng/g] (d7)	27 CBZ std dev [±] (d7)	28 CBZ/dm [ng/g] (d14)	29 CBZ std dev [±] (d14)	30 CBZ/dm [ng/g] (d21)	31 CBZ std dev [±] (d21)	32 CBZ/dm [ng/g] (d28)	33 CBZ std dev [±] (d28)	34 CBZ/dm [ng/g] (d31)	35 CBZ std dev [±] (d31)	36 CBZ/dm [ng/g] (d38)	37 CBZ std dev [±] (d38)	38 LMS [min] (measured at day 28, end of ca...)	39 LMS std e [±] (measured at day 28, end of ca...)	40 G/H (measured at day 28, end of ca...)	41 G/H std e [±] (measured at day 28, end of ca...)	42 Phago [%] (measured at day 28, end of ca...)	43 Phago std e [±] (measured at day 28, end of ca...)	44 AChE/prot [nmol/min/mg] (measured at day 28, end of ca...)	45 AChE/prot std e [±] (measured at day 28, end of ca...)	46 Hem MN [‰] (measured at day 28, end of ca...)	47 MN std e [±] (measured at day 28, end of ca...)	48 DNA fragm [%] (measured at day 28, end of ca...)	49 DNA fragm std e [±] (measured at day 28, end of ca...)	50 Lipo [intensity/µm²] (measured at day 28, end of ca...)	51 Lipo std e [±] (measured at day 28, end of ca...)	52 NL [intensity/µm²] (measured at day 28, end of ca...)	53 NL std e [±] (measured at day 28, end of ca...)	54 MDA [nmol/g] (measured at day 28, end of ca...)	55 MDA std e [±] (measured at day 28, end of ca...)	56 ACOX/prot [nmol/min/mg] (measured at day 28, end of ca...)	57 ACOX/prot std e [±] (measured at day 28, end of ca...)	58 CAT/prot [µmol/min/mg] (measured at day 28, end of ca...)	59 CAT/prot std e [±] (measured at day 28, end of ca...)	60 GST/prot [nmol/min/mg] (measured at day 28, end of ca...)	61 GST std e [±] (measured at day 28, end of ca...)	62 GR/prot [nmol/min/mg] (measured at day 28, end of ca...)	63 GR std e [±] (measured at day 28, end of ca...)	64 TGSH [µmol/g] (measured at day 28, end of ca...)	65 TGSH std e [±] (measured at day 28, end of ca...)	66 GPxSe-Dep/prot [nmol/min/mg] (measured at day 28, end of ca...)	67 GPxSe-Dep/prot std e [±] (measured at day 28, end of ca...)	68 GPxTOT/prot [nmol/min/mg] (measured at day 28, end of ca...)	69 GPxTOT std e [±] (measured at day 28, end of ca...)	70 TOSC-ROO [U/mg] (measured at day 28, end of ca...)	71 TOSC-ROO std e [±] (measured at day 28, end of ca...)	72 TOSC-HO [U/mg] (measured at day 28, end of ca...)	73 TOSC-HO std e [±] (measured at day 28, end of ca...)	74 TOSC-ONOOH [U/mg] (measured at day 28, end of ca...)	75 TOSC-ONOOH std e [±] (measured at day 28, end of ca...)	76 CSC flag (Calculated using seacarb afte...)	77 pH (total scale, Calculated using...)	78 CO2 [µmol/kg] (Calculated using seacarb afte...)	79 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	80 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	81 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	82 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	83 DIC [µmol/kg] (Calculated using seacarb afte...)	84 Omega Arg (Calculated using seacarb afte...)	85 Omega Cal (Calculated using seacarb afte...)
Laboratory	Mytilus galloprovincialis (mollusk)	140481	marinespecies.org	38	CONTROL	CTRL	35	0.5	13.0	0.2	8.12	0.02	3318.7	42.2	374.1	15.1	7.01	0.05	4.48	0.03	37.7	7.5	37.49	3.75	16.84	27.38	25.03	4.55	<1.03		<1.03		<1.03		<1.03		87.56625	2.302965819	7.173076923	0.699031530	68.58910891	2.270964626	45.47921288	8.106925203	0.375000063	0.125000063	32.62946923	1.254706073	47.04302240	2.852476815	70.08841322	11.572752380	149.7317530	49.06351806	0.467250625	0.131438366	14.39254262	2.040088994	71.97544506	4.460686531	15.34133126	0.878718805	0.434823944	0.043784817	4.621260783	0.434549060	8.837571602	2.601229093	332.9585537	58.31542972	497.7552253	16.75962249	434.6505867	19.62566927	26	8.01	25.38	637.13	639.50	2849.53	200.99	3075.9	3.06	4.79
Laboratory	Mytilus galloprovincialis (mollusk)	140481	marinespecies.org	38	CARBAMAZEPINE	CBZ	35	0.5	13.1	0.2	8.13	0.02	3308.3	34.7	369.3	9.3	7.03	0.05	4.50	0.03	37.7	7.5	118.20	30.24	63.81	15.62	66.69	42.47	61.99	33.6	46.70	21.33	22.89	7.77	23.77	39.39	68.25000	3.689794484	3.629619261	0.299315063	49.76169676	6.802527722	158.26841390	9.158463507	0.875000000	0.314576435	27.06364354	3.644795697	65.02748707	4.441970001	146.71717770	8.475782939	167.4859638	71.71132120	0.491103215	0.086031151	14.31286583	2.679452917	93.71124019	9.707951530	20.36251890	4.051501138	0.333638498	0.113573650	4.059918230	0.334032668	11.744467170	2.049819008	443.6294715	62.39502380	530.2634863	104.10844520	458.3100931	36.63252089	26	8.02	24.65	618.64	620.94	2831.12	204.33	3060.1	3.11	4.87
Laboratory	Mytilus galloprovincialis (mollusk)	140481	marinespecies.org	38	ACIDIFICATION	ACD	35	0.5	13.0	0.1	7.62	0.02	3315.0	37.5	1704.9	107.6	2.14	0.07	1.37	0.05	37.7	7.5	60.85	52.13	9.48	14.64	<1.03		<1.03		<1.03		<1.03		<1.03		84.47625	4.712259886	6.666666667	1.452966315	76.69076228	3.345254660	103.56159270	1.446598075	1.375000125	0.239356799	31.82082972	3.582929345	72.12615993	11.583312490	279.83231620	2.632343942	115.2464130	31.23848242	0.396672188	0.018704977	15.68976188	3.443217374	51.65594261	2.718971290	17.39251543	3.595399637	0.400018139	0.110041188	2.689692546	0.119029606	9.120943250	1.467425016	473.0142022	71.69165406	606.5732196	56.68505981	516.4639244	40.12394916	26	7.51	88.86	2230.46	2238.77	3150.94	70.20	3310.0	1.07	1.67
Laboratory	Mytilus galloprovincialis (mollusk)	140481	marinespecies.org	38	ACIDIFICATION + CARBAMAZEPINE	ACD+CBZ	35	0.5	13.1	0.1	7.63	0.03	3314.0	18.7	1719.1	123.6	2.13	0.14	1.36	0.09	37.7	7.5	107.90	56.66	104.70	41.35	51.02	4.37	108.90	48.9	42.27	18.70	50.68	3.10	41.42	38.83	59.96875	4.923470310	4.021008403	1.094850873	41.60818288	7.008404532	119.25163940	1.689085531	1.375187594	0.314601337	28.71867482	6.138295954	113.02158350	8.308426739	351.81805390	71.202754320	149.1079226	55.45215311	0.530672064	0.084343845	11.53985008	1.382813982	73.94330748	3.414610227	13.31807082	4.493999289	0.418912719	0.062262020	4.996063557	0.360988579	5.854716488	1.474449927	414.0403070	48.60650242	446.0013212	22.02000000	447.9654418	34.02355077	26	7.52	86.66	2175.29	2183.40	3146.27	71.77	3304.7	1.09	1.71