Citation:

PANGAEA.930305

Name: Seawater carbonate chemistry and ROS and EPS production of the Trichodesmium erythraeum
Published: 2021-04-14
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bacteria; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Other studied parameter or process; Pelagos; Phytoplankton; Single species; Trichodesmium erythraeum

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Abstract:

The diazotrophic cyanobacterium Trichodesmium is thought to be a major contributor to the new N in the parts of the oligotrophic, subtropical and tropical oceans. In this study physiological and biochemical methods and transcriptome sequencing were used to investigate the influences of ocean acidification (OA) on Trichodesmium erythraeum (T. erythraeum). We presented evidence that OA caused by CO2 slowed the growth rate and physiological activity of T. erythraeum. OA led to reduced development of proportion of the vegetative cells into diazocytes which included up‐regulated genes of nitrogen fixation. Reactive oxygen species (ROS) accumulation was increased due to the disruption of photosynthetic electron transport and decrease in antioxidant enzyme activities under acidified conditions. This study showed that OA increased the amounts of (exopolysaccharides) EPS in T. erythraeum, and the key genes of ribose‐5‐phosphate (R5P) and glycosyltransferases (Tery_3818) were up‐regulated. These results provide new insight into how ROS and EPS of T. erythraeum increase in an acidified future ocean to cope with OA‐imposed stress.

Keyword(s):

Bacteria; Bottles or small containers/Aquaria (<20 L); Cyanobacteria; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Other studied parameter or process; Pelagos; Phytoplankton; Single species; Trichodesmium erythraeum

Supplement to:

Wu, Shijie; Mi, Tiezhu; Zhen, Yu; Yu, Elizabeth K; Wang, Fuwen; Yu, Zhigang; Mock, Timothy D (2021): A rise in ROS and EPS production: new insights into the Trichodesmium erythraeum response to ocean acidification. Journal of Phycology, 57(1), 172-182, https://doi.org/10.1111/jpy.13075

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-04-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Zhen, Yu		study
2	Species	Species		Zhen, Yu
3	Registration number of species	Reg spec no		Zhen, Yu
4	Uniform resource locator/link to reference	URL ref		Zhen, Yu		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Zhen, Yu
6	Treatment	Treat		Zhen, Yu
7	Superoxide dismutase activity, unit per protein mass	SOD/prot	U/mg	Zhen, Yu
8	Catalase activity, per protein mass	CAT/prot	µkat/mg	Zhen, Yu
9	Salinity	Sal		Zhen, Yu
10	Temperature, water	Temp	°C	Zhen, Yu
11	Reactive oxygen species	ROS		Zhen, Yu
12	Reactive oxygen species, standard deviation	ROS std dev	±	Zhen, Yu
13	Exopolysaccharides	EPS	µg/l	Zhen, Yu
14	Exopolysaccharides, standard deviation	EPS std dev	±	Zhen, Yu
15	Growth rate	µ	1/day	Zhen, Yu
16	Growth rate, standard deviation	µ std dev	±	Zhen, Yu
17	Chlorophyll a per cell	Chl a/cell	pg/#	Zhen, Yu
18	Chlorophyll a, standard deviation	Chl a std dev	±	Zhen, Yu
19	Maximum photochemical quantum yield of photosystem II	Fv/Fm		Zhen, Yu
20	Maximum photochemical quantum yield of photosystem II, standard deviation	Fv/Fm std dev	±	Zhen, Yu
21	Carbon, organic, particulate, per cell	POC/cell	pmol/#	Zhen, Yu
22	Carbon, organic, particulate, standard deviation	POC std dev	±	Zhen, Yu
23	Particulate organic nitrogen per cell	PON/cell	pmol/#	Zhen, Yu
24	Nitrogen, organic, particulate, per cell, standard deviation	PON/cell std dev	±	Zhen, Yu
25	pH	pH		Zhen, Yu	Potentiometric	NBS scale
26	pH, standard deviation	pH std dev	±	Zhen, Yu	Potentiometric	NBS scale
27	Alkalinity, total	AT	µmol/kg	Zhen, Yu	Potentiometric titration
28	Alkalinity, total, standard deviation	AT std dev	±	Zhen, Yu	Potentiometric titration
29	Carbon, inorganic, dissolved	DIC	µmol/kg	Zhen, Yu	Calculated using CO2SYS
30	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Zhen, Yu	Calculated using CO2SYS
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Zhen, Yu	Calculated using CO2SYS
32	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Zhen, Yu	Calculated using CO2SYS
33	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
35	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

84 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Treat	7 SOD/prot [U/mg]	8 CAT/prot [µkat/mg]	9 Sal	10 Temp [°C]	11 ROS	12 ROS std dev [±]	13 EPS [µg/l]	14 EPS std dev [±]	15 µ [1/day]	16 µ std dev [±]	17 Chl a/cell [pg/#]	18 Chl a std dev [±]	19 Fv/Fm	20 Fv/Fm std dev [±]	21 POC/cell [pmol/#]	22 POC std dev [±]	23 PON/cell [pmol/#]	24 PON/cell std dev [±]	25 pH (NBS scale, Potentiometric)	26 pH std dev [±] (NBS scale, Potentiometric)	27 AT [µmol/kg] (Potentiometric titration)	28 AT std dev [±] (Potentiometric titration)	29 DIC [µmol/kg] (Calculated using CO2SYS)	30 DIC std dev [±] (Calculated using CO2SYS)	31 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	32 pCO2 std dev [±] (Calculated using CO2SYS)	33 CSC flag (Calculated using seacarb afte...)	34 pH (total scale, Calculated using...)	35 CO2 [µmol/kg] (Calculated using seacarb afte...)	36 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	37 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	38 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	39 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	40 DIC [µmol/kg] (Calculated using seacarb afte...)	41 Omega Arg (Calculated using seacarb afte...)	42 Omega Cal (Calculated using seacarb afte...)
laboratory	Trichodesmium erythraeum (prokaryotes)	660919	marinespecies.org	30	Ambient	21.52	8.89	33	26	91.20	9.62	51.71	0.53	0.34	0.03	0.90	0.05	0.35	0.02	2.58	0.16	0.46	0.02	8.03	0.03	2275	46	2050	31	601	58	26	7.89	17.14	612.75	614.69	1892.14	156.91	2066.20	2.53	3.85
laboratory	Trichodesmium erythraeum (prokaryotes)	660919	marinespecies.org	30	Acidified conditions	18.33	1.89	33	26	213.67	24.66	176.22	1.11	0.29	0.03	0.72	0.03	0.23	0.04	0.95	0.11	0.15	0.03	7.77	0.02	2292	23	2180	29	1251	25	26	7.63	34.00	1215.20	1219.04	2063.13	94.07	2191.20	1.52	2.31