Adam Nicole nadam@geomar.de 0000-0002-4928-3924 Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 2021 Hydrogen uptake activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge during METEOR cruise M78/2 dataset https://doi.org/10.1594/PANGAEA.930105 2021-04-07T15:55:32 Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogen uptake (hydrogenase) activity by means of an activity-based screen. The hydrogen uptake activity of positively screened clones was determined by means of a spectrophotometric assay and the DNA-sequences of the respective fosmid inserts were determined by PacBio Sequencing. The dataset contain hydrogen-uptake activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields. Adam Nicole nadam@geomar.de 0000-0002-4928-3924 Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 2018 Novel hydrogenases from deep-sea hydrothermal vent metagenomes identified by a recently developed activity-based screen journal article The ISME Journal 12(5) https://doi.org/10.1038/s41396-017-0040-6 1225-1236 -13.508833333332973 -12.37233329999998 -9.5438333333333 -4.804 -13.031333322221997 -7.548333333333333 2009-04-17T18:08:00 2009-04-30T11:50:00 -2980.0 -1519.0 not specified M78/2_274 -4.804 -12.3723333 -2980.0 2009-04-17T18:08:00 M78/2 M78/2 MARSUED5 MARSUED5 hdl:10013/epic.36797.d001 Port of Spain, Trinidad and Tobago 2009-04-01T00:00:00 2009-04-01 Rio de Janeiro, Brazil 2009-05-12T00:00:00 2009-05-12 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Seifert, Richard Seifert, Richard doi:10.2312/cr_m78_2 20090245 hdl:10013/epic.44784.d030 hdl:10013/epic.44803.d121 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Remote operated vehicle ROV M78/2_314 -8.2971666666667 -13.508833333333 -2886.0 2009-04-27T10:01:00 M78/2 M78/2 MARSUED5 MARSUED5 hdl:10013/epic.36797.d001 Port of Spain, Trinidad and Tobago 2009-04-01T00:00:00 2009-04-01 Rio de Janeiro, Brazil 2009-05-12T00:00:00 2009-05-12 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Seifert, Richard Seifert, Richard doi:10.2312/cr_m78_2 20090245 hdl:10013/epic.44784.d030 hdl:10013/epic.44803.d121 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Remote operated vehicle ROV M78/2_324 -9.5438333333333 -13.212833333333 -1519.0 2009-04-30T11:50:00 M78/2 M78/2 MARSUED5 MARSUED5 hdl:10013/epic.36797.d001 Port of Spain, Trinidad and Tobago 2009-04-01T00:00:00 2009-04-01 Rio de Janeiro, Brazil 2009-05-12T00:00:00 2009-05-12 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Seifert, Richard Seifert, Richard doi:10.2312/cr_m78_2 20090245 hdl:10013/epic.44784.d030 hdl:10013/epic.44803.d121 Meteor (1986) https://en.wikipedia.org/wiki/RV_Meteor_(1986) DBBH 8411279 Remote operated vehicle ROV 32 CC-BY-4.0 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ Metagenomic fosmid libraries were constructed from the environmental sample and screened for hydrogen uptake activity. The hydrogen upake activity of the fosmid clones has been determined as described in the cited publication. Event label Event Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Event Area/locality Area Area http://dbpedia.org/resource/Area Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Area Sample ID Sample ID Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Clone Sample ID (Clone) Hydrogen uptake activity H2 upt act nmol/min/mg Specific Activity http://search.ebscohost.com/login.aspx?direct=true&db=nlebk&AN=98508&site=ehost-live&ebv=EB&ppid=pp_610 Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 at 25°C H2 upt act [nmol/min/mg] (at 25°C) Hydrogen uptake activity, standard deviation H2 upt act std dev ± standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 at 25°C H2 upt act std dev [±] (at 25°C) Hydrogen uptake activity H2 upt act nmol/min/mg Specific Activity http://search.ebscohost.com/login.aspx?direct=true&db=nlebk&AN=98508&site=ehost-live&ebv=EB&ppid=pp_610 Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 at 55°C H2 upt act [nmol/min/mg] (at 55°C) Hydrogen uptake activity, standard deviation H2 upt act std dev ± standard deviation std dev https://en.wikipedia.org/wiki/Standard_deviation Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 at 55°C H2 upt act std dev [±] (at 55°C) Accession number, genetics Access no gen accession number Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Access no gen Sample method Sample method Perner Mirjam mperner@geomar.de 0000-0003-1223-9310 Sample method CurationLevelC Enhanced curation https://wiki.pangaea.de/wiki/Curation_levels citable function-based screen Hydrogenase hydrothermal vent Metagenome geomar author73528 author75058 basis2 campaign3391645 campaign34047 curlevel30 event2621046 event2621085 event2621095 geocode1599 geocode1600 geocode1601 geocode8128 journal7763 license21 method11520 param153190 param15373 param16065 param16119 param192827 param192828 pi73528 ref107851 term242 term37764 term38742 term43770 term44032 term53736 term68002 term68003 term68005 term77733 term77900 topotype1