de.pangaea.dataset930105 und dataset PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact 2026-04-29T17:07:29Z https://doi.pangaea.de/10.1594/PANGAEA.930105 <gco:CharacterString>Hydrogen uptake activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge during METEOR cruise M78/2</gco:CharacterString> 2021-04-07T15:55:32 publication https://doi.org/10.1594/PANGAEA.930105 Adam, Nicole
nadam@geomar.de
https://orcid.org/0000-0002-4928-3924 information author Perner, Mirjam
mperner@geomar.de
https://orcid.org/0000-0003-1223-9310 information author PANGAEA
info@pangaea.de
https://www.pangaea.de/ information publisher tableDigital Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogen uptake (hydrogenase) activity by means of an activity-based screen. The hydrogen uptake activity of positively screened clones was determined by means of a spectrophotometric assay and the DNA-sequences of the respective fosmid inserts were determined by PacBio Sequencing. The dataset contain hydrogen-uptake activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields. completed Perner, Mirjam
mperner@geomar.de
https://orcid.org/0000-0003-1223-9310 information principalInvestigator function-based screen Hydrogenase hydrothermal vent Metagenome theme <gco:CharacterString>PANGAEA Keyword List</gco:CharacterString> 1995-01-01 creation PANGAEA
info@pangaea.de
https://www.pangaea.de/ information pointOfContact copyright copyright CC-BY-4.0: Creative Commons Attribution 4.0 International textTable und geoscientificInformation -13.508833333332973 -12.37233329999998 -9.5438333333333 -4.804 2009-04-17T18:08:00 2009-04-30T11:50:00 Metagenomic fosmid libraries were constructed from the environmental sample and screened for hydrogen uptake activity. The hydrogen upake activity of the fosmid clones has been determined as described in the cited publication. Event label thematicClassification Area/locality thematicClassification Sample ID thematicClassification Clone Hydrogen uptake activity physicalMeasurement at 25°C nmol/min/mg nmol/min/mg Hydrogen uptake activity, standard deviation physicalMeasurement at 25°C ± ± Hydrogen uptake activity physicalMeasurement at 55°C nmol/min/mg nmol/min/mg Hydrogen uptake activity, standard deviation physicalMeasurement at 55°C ± ± Accession number, genetics thematicClassification Sample method thematicClassification text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.930105?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data. EVENT LABEL: M78/2_274 * LATITUDE: -4.804000 * LONGITUDE: -12.372333 * DATE/TIME: 2009-04-17T18:08:00 * ELEVATION: -2980.0 m * CAMPAIGN: M78/2 * BASIS: Meteor (1986) * METHOD/DEVICE: Remote operated vehicle 2009-04-17T18:08:00 EVENT LABEL: M78/2_314 * LATITUDE: -8.297167 * LONGITUDE: -13.508833 * DATE/TIME: 2009-04-27T10:01:00 * ELEVATION: -2886.0 m * CAMPAIGN: M78/2 * BASIS: Meteor (1986) * METHOD/DEVICE: Remote operated vehicle 2009-04-27T10:01:00 EVENT LABEL: M78/2_324 * LATITUDE: -9.543833 * LONGITUDE: -13.212833 * DATE/TIME: 2009-04-30T11:50:00 * ELEVATION: -1519.0 m * CAMPAIGN: M78/2 * BASIS: Meteor (1986) * METHOD/DEVICE: Remote operated vehicle 2009-04-30T11:50:00 Supplement to <gco:CharacterString>Novel hydrogenases from deep-sea hydrothermal vent metagenomes identified by a recently developed activity-based screen</gco:CharacterString> 2018-01-01 publication https://doi.org/10.1038/s41396-017-0040-6 Adam, Nicole
nadam@geomar.de
https://orcid.org/0000-0002-4928-3924 information author Perner, Mirjam
mperner@geomar.de
https://orcid.org/0000-0003-1223-9310 information author documentDigital The ISME Journal 12(5) 1225-1236