PANGAEA_930105
Hydrogen uptake activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge during METEOR cruise M78/2
Adam, Nicole; Perner, Mirjam
Hydrogen uptake activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge during METEOR cruise M78/2
2021-04-07
PANGAEA
Dataset
https://doi.pangaea.de/10.1594/PANGAEA.930105
Investigator
Mirjam
Perner
mperner@geomar.de
Earth Science
Event label
Area/locality
Sample ID
Hydrogen uptake activity
Hydrogen uptake activity, standard deviation
Accession number, genetics
Sample method
geoscientificInformation
function-based screen
Hydrogenase
hydrothermal vent
M78/2
M78/2_274
M78/2_314
M78/2_324
Metagenome
Remote operated vehicle
Meteor (1986)
2009-04-17
2009-04-30
Complete
-9.5438333333333
-4.804
-13.508833333332973
-12.37233329999998
unrestricted
CC-BY-4.0: Creative Commons Attribution 4.0 International
English
PANGAEA
Data Publisher for Earth & Environmental Science
https://www.pangaea.de/
Data Center Contact
Michael
Diepenbroek
info@pangaea.de
Leobener Str.
Bremen
Bremen
28359
Germany
online
32 data points
text/tab-separated-values
Adam, Nicole; Perner, Mirjam (2018): Novel hydrogenases from deep-sea hydrothermal vent metagenomes identified by a recently developed activity-based screen. The ISME Journal, 12(5), 1225-1236, https://doi.org/10.1038/s41396-017-0040-6
Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogen uptake (hydrogenase) activity by means of an activity-based screen. The hydrogen uptake activity of positively screened clones was determined by means of a spectrophotometric assay and the DNA-sequences of the respective fosmid inserts were determined by PacBio Sequencing. The dataset contain hydrogen-uptake activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.930105"! ** Metagenomic fosmid libraries were constructed from the environmental sample and screened for hydrogen uptake activity. The hydrogen upake activity of the fosmid clones has been determined as described in the cited publication.
https://doi.org/10.1038/s41396-017-0040-6
Novel hydrogenases from deep-sea hydrothermal vent metagenomes identified by a recently developed activity-based screen
https://en.wikipedia.org/wiki/RV_Meteor_(1986)
Meteor (1986)
https://hdl.handle.net/10013/epic.36797.d001
M78/2
DIF
9.4
2021-04-07
2026-04-29