Citation:

PANGAEA.930105

Name: Hydrogen uptake activities of metagenomic fosmid clones derived from hydrothermal vent systems along the Mid-Atlantic Ridge during METEOR cruise M78/2
Published: 2021-04-07
License: https://creativecommons.org/licenses/by/4.0/
Keywords: function-based screen; Hydrogenase; hydrothermal vent; Metagenome

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Published: 2021-04-07 • DOI registered: 2022-01-06

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Abstract:

Metagenomic fosmid libraries were constructed from hydrothermal chimneys and fluids and screened for hydrogen uptake (hydrogenase) activity by means of an activity-based screen. The hydrogen uptake activity of positively screened clones was determined by means of a spectrophotometric assay and the DNA-sequences of the respective fosmid inserts were determined by PacBio Sequencing. The dataset contain hydrogen-uptake activities of fosmid clones from the Sisters Peak, Nibelungen and Lilliput hydrothermal vent fields.

Keyword(s):

function-based screen; Hydrogenase; hydrothermal vent; Metagenome

Supplement to:

Adam, Nicole; Perner, Mirjam (2018): Novel hydrogenases from deep-sea hydrothermal vent metagenomes identified by a recently developed activity-based screen. The ISME Journal, 12(5), 1225-1236, https://doi.org/10.1038/s41396-017-0040-6

Coverage:

Median Latitude: -7.548333 * Median Longitude: -13.031333 * South-bound Latitude: -9.543833 * West-bound Longitude: -13.508833 * North-bound Latitude: -4.804000 * East-bound Longitude: -12.372333

Date/Time Start: 2009-04-17T18:08:00 * Date/Time End: 2009-04-30T11:50:00

Minimum Elevation: -2980.0 m * Maximum Elevation: -1519.0 m

Event(s):

M78/2_274 * Latitude: -4.804000 * Longitude: -12.372333 * Date/Time: 2009-04-17T18:08:00 * Elevation: -2980.0 m * Campaign: (MARSUED5) * Basis: * Method/Device: Remote operated vehicle (ROV)

M78/2_314 * Latitude: -8.297167 * Longitude: -13.508833 * Date/Time: 2009-04-27T10:01:00 * Elevation: -2886.0 m * Campaign: (MARSUED5) * Basis: * Method/Device: Remote operated vehicle (ROV)

M78/2_324 * Latitude: -9.543833 * Longitude: -13.212833 * Date/Time: 2009-04-30T11:50:00 * Elevation: -1519.0 m * Campaign: (MARSUED5) * Basis: * Method/Device: Remote operated vehicle (ROV)

Comment:

Metagenomic fosmid libraries were constructed from the environmental sample and screened for hydrogen uptake activity. The hydrogen upake activity of the fosmid clones has been determined as described in the cited publication.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Comment
1	Event label	Event		Perner, Mirjam
2	Area/locality	Area		Perner, Mirjam
3	Sample ID	Sample ID		Perner, Mirjam	Clone
4	Hydrogen uptake activity	H2 upt act	nmol/min/mg	Perner, Mirjam	at 25°C
5	Hydrogen uptake activity, standard deviation	H2 upt act std dev	±	Perner, Mirjam	at 25°C
6	Hydrogen uptake activity	H2 upt act	nmol/min/mg	Perner, Mirjam	at 55°C
7	Hydrogen uptake activity, standard deviation	H2 upt act std dev	±	Perner, Mirjam	at 55°C
8	Accession number, genetics	Access no gen		Perner, Mirjam
9	Sample method	Sample method		Perner, Mirjam

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

32 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Area	3 Sample ID (Clone)	4 H2 upt act [nmol/min/mg] (at 25°C)	5 H2 upt act std dev [±] (at 25°C)	6 H2 upt act [nmol/min/mg] (at 55°C)	7 H2 upt act std dev [±] (at 55°C)	8 Access no gen	9 Sample method
M78/2_324	Lilliput hydrothermal field	Lilli33G1	66.36	13.13	257.60	18.51	insdc:MG456606	Diffuse hydrothermal fluids were collected from the Lilliput vent field using the KIPS (Kiel Pumping System, Garbe-Schönberg et al. 2006).
M78/2_314	Nibelungen hydrothermal field	Nib22E5	0.00	0.00	84.29	3.00	insdc:MG456605	Hydrothermal fluids were collected from Dragon''s throat crater using the KIPS (Kiel Pumping System, Garbe-Schönberg et al. 2006).
M78/2_274	Sisters Peak hydrothermal field	SP11A2	0.00	0.00	157.04	3.87	insdc:MG456603	Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV.
M78/2_274	Sisters Peak hydrothermal field	SP11F2	0.00	0.00	174.57	10.53	insdc:MG456604	Beehive-like material from the Sisters Peak chimney structure was collected by means of the ROV.