Citation:

PANGAEA.929195

Name: Seawater carbonate chemistry and sperm motility of Tegillarca granosavia
Published: 2021-03-16
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Pacific; Other metabolic rates; Single species; Tegillarca granosa; Temperate

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Abstract:

Since the industrial revolution, massive amount of anthropogenic carbon dioxide (CO2) have been generated to elevate the atmospheric CO2 concentration. Some anthropogenic CO2 have been absorbed by the ocean to cause “ocean acidification” (OA). Although the negative impacts of OA on sperm motility are increasingly found in various marine invertebrate species, the cellular and molecular mechanisms for these effects are still poorly understood. This study investigated the effect of OA (pH7.8 and 7.4) on sperm motility and energy supplying pathway in blood clam Tegillarca granosa. The results showed that the sperm swimming speed reduced significantly in acidified seawater. Since the adenosine triphosphate (ATP) level of sperm is closely related to its motility, we analyzed the sperm ATP content and activities of key enzymes during ATP synthesis under different OA scenarios. OA treatments significantly reduced ATP content as well as activities of 6-phosphofructokinase and pyruvate kinase in the sperm of T. granosa. The sperm Ca2+-ATPase of various animals has been reported to regulate sperm motility. Therefore, we explored the Ca2+-ATPase activity of T. granosa sperm under OA treatment. The results found that Ca2+-ATPase activities in the sperm of T. granosa were significantly declined under OA scenarios. In conclusion, these results suggested that OA could constrain sperm motility through inhibiting ATP synthesis and disturbing intracellular Ca2+ regulation.

Keyword(s):

Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Pacific; Other metabolic rates; Single species; Tegillarca granosa; Temperate

Supplement to:

Shi, Wei; Han, Yu; Huang, Xianke; Hu, Lihua; Chai, Xueliang; Liu, Guangxu (2020): Ocean acidification inhibits the sperm motility of Tegillarca granosavia disturbing ATP synthesis and intracellular CA2+ activity. Acta Hydrobiologica Sinica, 44, 570-576, https://doi.org/10.7541/2020.070

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 28.283500 * Longitude: 121.244300

Event(s):

Yueqing_Bay_OA * Latitude: 28.283500 * Longitude: 121.244300 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Guangxu		study
2	Species	Species		Liu, Guangxu
3	Registration number of species	Reg spec no		Liu, Guangxu
4	Uniform resource locator/link to reference	URL ref		Liu, Guangxu		WoRMS Aphia ID
5	Treatment	Treat		Liu, Guangxu
6	Adenosine triphosphate, per unit protein	ATP	nmol/mg	Liu, Guangxu
7	6-phosphofructokinase activity, per protein	PFK act	U/g	Liu, Guangxu
8	Pyruvate kinase activity, per protein	PK act	U/g	Liu, Guangxu
9	Calcium-ATPase activity	Ca2+-ATPase act	U/mg	Liu, Guangxu
10	Curvilinear velocity	VCL	µm/s	Liu, Guangxu
11	Curvilinear velocity, standard error	VCL std e	±	Liu, Guangxu
12	Average path velocity	VAP	µm/s	Liu, Guangxu
13	Average path velocity, standard error	VAP std e	±	Liu, Guangxu
14	Straight line velocity	VSL	µm/s	Liu, Guangxu
15	Straight line velocity, standard error	VSL std e	±	Liu, Guangxu
16	Temperature, water	Temp	°C	Liu, Guangxu
17	Temperature, water, standard error	T std e	±	Liu, Guangxu
18	Salinity	Sal		Liu, Guangxu
19	Salinity, standard error	Sal std e	±	Liu, Guangxu
20	pH	pH		Liu, Guangxu	Potentiometric	NBS scale
21	pH, standard error	pH std e	±	Liu, Guangxu	Potentiometric	NBS scale
22	Alkalinity, total	AT	µmol/kg	Liu, Guangxu	Potentiometric titration
23	Alkalinity, total, standard error	AT std e	±	Liu, Guangxu	Potentiometric titration
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Liu, Guangxu	Calculated using CO2SYS
25	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Liu, Guangxu	Calculated using CO2SYS
26	Carbon, inorganic, dissolved	DIC	µmol/kg	Liu, Guangxu	Calculated using CO2SYS
27	Carbon, inorganic, dissolved, standard error	DIC std e	±	Liu, Guangxu	Calculated using CO2SYS
28	Aragonite saturation state	Omega Arg		Liu, Guangxu	Calculated using CO2SYS
29	Aragonite saturation state, standard error	Omega Arg std e	±	Liu, Guangxu	Calculated using CO2SYS
30	Calcite saturation state	Omega Cal		Liu, Guangxu	Calculated using CO2SYS
31	Calcite saturation state, standard error	Omega Cal std e	±	Liu, Guangxu	Calculated using CO2SYS
32	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
34	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1263 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 ATP [nmol/mg]	7 PFK act [U/g]	8 PK act [U/g]	9 Ca2+-ATPase act [U/mg]	10 VCL [µm/s]	11 VCL std e [±]	12 VAP [µm/s]	13 VAP std e [±]	14 VSL [µm/s]	15 VSL std e [±]	16 Temp [°C]	17 T std e [±]	18 Sal	19 Sal std e [±]	20 pH (NBS scale, Potentiometric)	21 pH std e [±] (NBS scale, Potentiometric)	22 AT [µmol/kg] (Potentiometric titration)	23 AT std e [±] (Potentiometric titration)	24 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	25 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	26 DIC [µmol/kg] (Calculated using CO2SYS)	27 DIC std e [±] (Calculated using CO2SYS)	28 Omega Arg (Calculated using CO2SYS)	29 Omega Arg std e [±] (Calculated using CO2SYS)	30 Omega Cal (Calculated using CO2SYS)	31 Omega Cal std e [±] (Calculated using CO2SYS)	32 CSC flag (Calculated using seacarb afte...)	33 pH (total scale, Calculated using...)	34 CO2 [µmol/kg] (Calculated using seacarb afte...)	35 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	36 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	37 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	38 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	39 DIC [µmol/kg] (Calculated using seacarb afte...)	40 Omega Arg (Calculated using seacarb afte...)	41 Omega Cal (Calculated using seacarb afte...)
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1	3565.217391										26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1	3130.434783										26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1	3500.000000										26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8	1869.565217										26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8	2521.739130										26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8	2021.739130										26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4	1413.043478										26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4	1021.739130										26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4	1195.652174										26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1		4501.607717									26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1		4501.607717									26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1		5401.929260									26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8		3462.775167									26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8		3809.052684									26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8		3809.052684									26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4		2400.857449									26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4		2100.750268									26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4		2100.750268									26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1			371.4362272								26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1			365.0321543								26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1			365.0321543								26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8			249.7588424								26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8			217.7384780								26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8			268.9710611								26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4			166.5058950								26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4			192.1221865								26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4			192.1221865								26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1				32.45967742							26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1				30.57795699							26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1				32.93010753							26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8				30.57795699							26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8				33.87096774							26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8				25.87365591							26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4				18.34677419							26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4				10.81989247							26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4				16.93548387							26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH8.1					123.63	2.06	136.30	3.23	105.65	5.39	26.28	0.05	21.19	0.08	8.07	0.07	2057.63	11.25	596.37	3.25	1954.68	6.87	2.04	0.02	3.26	0.03	26	7.92	17.37	588.48	590.34	1784.14	116.18	1917.7	1.97	3.13
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.8					101.95	7.97	85.66	3.20	80.51	2.19	26.19	0.10	21.22	0.09	7.79	0.10	2062.97	11.28	1175.25	20.52	2004.80	8.41	1.16	0.02	1.85	0.02	26	7.64	35.45	1198.29	1202.07	1909.69	65.16	2010.3	1.10	1.76
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH7.4					82.73	3.87	72.64	3.20	57.75	4.31	26.22	0.11	21.18	0.12	7.41	0.05	2067.78	11.58	3095.58	43.70	2135.43	7.76	0.48	0.01	0.77	0.01	26	7.26	89.10	3013.26	3022.80	2000.94	28.46	2118.5	0.48	0.77