Citation:

PANGAEA.929192

Name: Seawater carbonate chemistry and gustation mediated-feeding behavior of black sea bream, Acanthopagrus schlegelii
Published: 2020
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acanthopagrus schlegelii; Animalia; Behaviour; Benthic animals; Benthos; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Laboratory experiment; North Pacific; Other studied parameter or process; Single species; Temperate

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Abstract:

Growing evidence suggests that ocean acidification (OA) may affect animal behaviors such as feeding. Although gustation plays a crucial role in evaluating the quality and palatability of food and ultimately influences whether or not teleosts consume the food, the potential impact of OA on gustation-mediated feeding behavior remains unknown. In this study, gustation mediated-feeding behavior, as indicated by the consumption rate (CR) and swallowing rate (SR) of agar pellets with or without feed upon OA exposure was investigated in black sea bream (Acanthopagrus schlegelii). Results showed that the exposure to acidified seawater led to significant reductions in the CR and SR of feed-containing agar pellets. In addition, the in vivo contents of three neurotransmitters and expression of genes from the gustatory signal transduction pathway were all significantly suppressed by the OA treatment. In general, the data obtained indicated that OA may hinder the gustation-mediated feeding behavior of A. schlegelii by disrupting gustatory signal transduction, which may aggravate the issue of food shortage for wild populations of black sea bream.

Keyword(s):

Acanthopagrus schlegelii; Animalia; Behaviour; Benthic animals; Benthos; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Laboratory experiment; North Pacific; Other studied parameter or process; Single species; Temperate

Supplement to:

Rong, Jiahuan; Tang, Y; Zha, Shanjie; Han, Yu; Shi, Wei; Liu, Guangxu (2020): Ocean acidification impedes gustation-mediated feeding behavior by disrupting gustatory signal transduction in the black sea bream, Acanthopagrus schlegelii. Marine Environmental Research, 162, 105182, https://doi.org/10.1016/j.marenvres.2020.105182

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Guangxu		study
2	Species	Species		Liu, Guangxu
3	Registration number of species	Reg spec no		Liu, Guangxu
4	Uniform resource locator/link to reference	URL ref		Liu, Guangxu		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Liu, Guangxu
6	Treatment	Treat		Liu, Guangxu
7	gamma-Aminobutyric acid	g-ABA	µmol/g	Liu, Guangxu
8	Acetylcholine	ACh	µg/g	Liu, Guangxu
9	5-hydroxytryptamine	5-HT	ng/ml	Liu, Guangxu
10	Gene expression, fold change, relative	Gene expression		Liu, Guangxu		IP3R
11	Gene expression, fold change, relative	Gene expression		Liu, Guangxu		T1R3
12	Gene expression, fold change, relative	Gene expression		Liu, Guangxu		TRPM5
13	Gene expression, fold change, relative	Gene expression		Liu, Guangxu		P2Y4
14	Consumption	Consmpt	%	Liu, Guangxu		agar pellets containing feed
15	Consumption, standard error	Consmpt std e	±	Liu, Guangxu		agar pellets containing feed
16	Consumption	Consmpt	%	Liu, Guangxu		blank agar pellets
17	Consumption, standard error	Consmpt std e	±	Liu, Guangxu		blank agar pellets
18	Swallowing rate	Swallow	%	Liu, Guangxu		gar pellets containing feed
19	Swallowing rate, standard error	Swallow std e	±	Liu, Guangxu		gar pellets containing feed
20	Swallowing rate	Swallow	%	Liu, Guangxu		blank agar pellets
21	Swallowing rate, standard error	Swallow std e	±	Liu, Guangxu		blank agar pellets
22	Temperature, water	Temp	°C	Liu, Guangxu
23	Temperature, water, standard error	T std e	±	Liu, Guangxu
24	Salinity	Sal		Liu, Guangxu
25	Salinity, standard error	Sal std e	±	Liu, Guangxu
26	pH	pH		Liu, Guangxu	Potentiometric	NBS scale
27	pH, standard error	pH std e	±	Liu, Guangxu	Potentiometric	NBS scale
28	Alkalinity, total	AT	µmol/kg	Liu, Guangxu	Potentiometric titration
29	Alkalinity, total, standard error	AT std e	±	Liu, Guangxu	Potentiometric titration
30	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Liu, Guangxu	Calculated using CO2SYS
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Liu, Guangxu	Calculated using CO2SYS
32	Carbon, inorganic, dissolved	DIC	µmol/kg	Liu, Guangxu	Calculated using CO2SYS
33	Carbon, inorganic, dissolved, standard error	DIC std e	±	Liu, Guangxu	Calculated using CO2SYS
34	Aragonite saturation state	Omega Arg		Liu, Guangxu	Calculated using CO2SYS
35	Aragonite saturation state, standard error	Omega Arg std e	±	Liu, Guangxu	Calculated using CO2SYS
36	Calcite saturation state	Omega Cal		Liu, Guangxu	Calculated using CO2SYS
37	Calcite saturation state, standard error	Omega Cal std e	±	Liu, Guangxu	Calculated using CO2SYS
38	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
40	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2028 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Treat	7 g-ABA [µmol/g]	8 ACh [µg/g]	9 5-HT [ng/ml]	10 Gene expression (IP3R)	11 Gene expression (T1R3)	12 Gene expression (TRPM5)	13 Gene expression (P2Y4)	14 Consmpt [%] (agar pellets containing feed)	15 Consmpt std e [±] (agar pellets containing feed)	16 Consmpt [%] (blank agar pellets)	17 Consmpt std e [±] (blank agar pellets)	18 Swallow [%] (gar pellets containing feed)	19 Swallow std e [±] (gar pellets containing feed)	20 Swallow [%] (blank agar pellets)	21 Swallow std e [±] (blank agar pellets)	22 Temp [°C]	23 T std e [±]	24 Sal	25 Sal std e [±]	26 pH (NBS scale, Potentiometric)	27 pH std e [±] (NBS scale, Potentiometric)	28 AT [µmol/kg] (Potentiometric titration)	29 AT std e [±] (Potentiometric titration)	30 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	31 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	32 DIC [µmol/kg] (Calculated using CO2SYS)	33 DIC std e [±] (Calculated using CO2SYS)	34 Omega Arg (Calculated using CO2SYS)	35 Omega Arg std e [±] (Calculated using CO2SYS)	36 Omega Cal (Calculated using CO2SYS)	37 Omega Cal std e [±] (Calculated using CO2SYS)	38 CSC flag (Calculated using seacarb afte...)	39 pH (total scale, Calculated using...)	40 CO2 [µmol/kg] (Calculated using seacarb afte...)	41 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	42 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	43 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	44 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	45 DIC [µmol/kg] (Calculated using seacarb afte...)	46 Omega Arg (Calculated using seacarb afte...)	47 Omega Cal (Calculated using seacarb afte...)
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	4.013292434	343.8636364	21.36726547													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	4.183708248	346.1363636	21.36726547													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	3.979209271	350.6818182	18.97205589													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	3.996250852	382.5000000	18.87225549													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	3.911042945	387.0454545	19.17165669													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	3.962167689	396.1363636	20.46906188													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	4.388207226	373.4090909	18.87225549													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	3.928084526	450.6818182	20.66866267													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	3.280504431	314.3181818	18.87225549													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	3.433878664	302.9545455	18.67265469													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	3.365712338	330.2272727	18.57285429													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1	3.399795501	298.4090909	19.67065868													23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	3.212338105	293.8636364	18.97205589													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	3.058963872	273.4090909	18.67265469													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	3.007839127	227.9545455	19.07185629													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	3.041922290	234.7727273	17.47504990													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	3.110088616	200.6818182	16.97604790													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	3.195296524	246.1363636	18.37325349													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	3.229379686	273.4090909	17.87425150													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	2.820381731	266.5909091	17.97405190													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	2.973755965	221.1363636	19.07185629													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	3.007839127	207.5000000	19.97005988													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	3.416837082	241.5909091	19.97005988													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8	2.871506476	230.2272727	19.57085828													23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	2.922631220	193.8636364	17.87425150													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	2.820381731	214.3181818	17.57485030													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	2.598841172	198.4090909	18.27345309													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	2.990797546	207.5000000	18.67265469													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	2.684049080	168.8636364	18.17365269													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	2.479550102	218.8636364	18.27345309													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	2.667007498	223.4090909	17.67465070													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	2.667007498	218.8636364	18.27345309													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	2.854464894	223.4090909	17.87425150													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	3.024880709	193.8636364	16.67664671													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	3.024880709	218.8636364	17.57485030													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4	2.667007498	216.5909091	18.37325349													23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1				1.172834949	1.128964405	1.257013375	1.193335743									23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1				0.852634892	0.885767519	0.795536484	0.837987135									23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1				0.803850991	1.113421618	0.920187651	0.965936329									23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1				1.244011653	0.898132373	1.086734863	1.035264924									23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1				1.257013375	1.035264924	1.061913804	1.090507733									23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1				0.795536484	0.965936329	0.816014485	0.917004043									23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8				0.133971683	0.694959110	0.466516496	0.433769344									23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8				0.144586023	0.657471380	1.205807828	0.537747195									23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8				0.129857388	0.568408487	0.210224104	0.320856474									23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8				0.111491065	0.630688704	0.208771980	0.125869444									23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8				0.078563336	0.901250463	0.176368726	0.315344352									23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8				0.087171479	0.876605721	0.141283674	0.955945318									23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4				0.111878134	0.218393224	0.309926925	0.311002913									23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4				0.038740866	0.198196034	0.590496331	0.203768083									23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4				0.178624267	0.501735874	0.255253031	0.145591698									23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4				0.093104841	0.267016352	0.158219574	0.231647015									23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4				0.068869070	0.204475515	0.180074608	0.127184962									23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4				0.107320680	0.444421341	0.144252344	0.308854659									23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH8.1								91.09	2.83	11.65	3.33	95.60	2.18	14.58	4.54	23.81	0.08	20.76	0.01	8.11	0.01	2050.55	3.49	348.75	11.34	1848.74	3.99	2.62	0.06	4.21	0.10	26	7.96	16.52	523.78	525.48	1778.57	115.61	1910.70	1.92	3.09
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.8								80.92	2.68	10.93	3.69	89.33	1.94	13.33	4.38	23.81	0.09	20.61	0.01	7.79	0.01	2066.71	3.13	813.71	19.20	1980.30	3.99	1.38	0.03	2.22	0.04	26	7.64	37.48	1187.39	1191.25	1926.56	59.66	2023.70	0.99	1.60
laboratory	Acanthopagrus schlegelii (fish)	293534	marinespecies.org	15	pH7.4								71.55	3.12	7.80	2.15	79.01	2.33	11.46	3.29	23.78	0.09	20.74	0.02	7.40	0.01	2080.67	4.30	2118.45	46.48	2098.42	5.21	0.60	0.01	0.97	0.02	26	7.25	96.33	3051.50	3061.44	2020.70	25.57	2142.60	0.43	0.68