Citation:

PANGAEA.928764

Name: Seawater carbonate chemistry and growth, biochemical, antioxidants, metabolic enzymes and hemocytes population of the shrimp Litopenaeus vannamei
Published: 2021-03-05
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Arthropoda; Behaviour; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Growth/Morphology; Indian Ocean; Laboratory experiment; Litopenaeus vannamei; Mortality/Survival; Other metabolic rates; Pelagos; Single species; Tropical; Zooplankton

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Abstract:

Acidification in the marine environment has become a global issue that creates serious threats to marine organisms. In the present study, we evaluated the effect of CO2 driven acidification on the shrimp Litopenaeus vannamei post-larvae (PL). L. vannamei PL were exposed to six different CO2 driven acidified seawater, such as pH 8.20 (control), pH 7.8 (IPCC-predicted ocean pH by 2100), 7.6, 7.4, 7.2 and 7.0 with corresponding pCO2 level of 380.66, 557.53, 878.55, 1355.48, 2129.46, and 3312.12 μatm for seven weeks. At the end of the acidification experiment, results revealed that survival, growth, feed index, biochemical constitutes, chitin, minerals (Na, K, and Ca), and hemocyte populations of shrimps were found to be significantly decreased in CO2 driven acidified seawater which indicates the negative impacts of acidified seawater on these parameters in L. vannamei. Further, the level of antioxidants, lipid peroxidation, and metabolic enzymes were significantly higher in the muscle of shrimps exposed to acidified seawater suggests that the L. vannamei under oxidative stress and metabolic stress. Among these various acidified seawater experiment, pH 7.6 to 7.0 produced a significantly adverse effect on shrimps. Hence, the present study concluded that the elevated level of seawater acidification can produce harmful effects on L. vannamei PL which leads to potential threats to shrimp species in the marine environment.

Keyword(s):

Animalia; Arthropoda; Behaviour; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Growth/Morphology; Indian Ocean; Laboratory experiment; Litopenaeus vannamei; Mortality/Survival; Other metabolic rates; Pelagos; Single species; Tropical; Zooplankton

Supplement to:

Muralisankar, T; Kalaivani, P; Thangal, Said Hamid; Santhanam, P (2021): Growth, biochemical, antioxidants, metabolic enzymes and hemocytes population of the shrimp Litopenaeus vannamei exposed to acidified seawater. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 239, 108843, https://doi.org/10.1016/j.cbpc.2020.108843

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James (2021): seacarb: seawater carbonate chemistry with R. R package version 3.2.16. https://cran.r-project.org/web/packages/seacarb/index.html

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-05.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Muralisankar, T		study
2	Species	Species		Muralisankar, T
3	Registration number of species	Reg spec no		Muralisankar, T
4	Uniform resource locator/link to reference	URL ref		Muralisankar, T		WoRMS Aphia ID
5	Treatment	Treat		Muralisankar, T
6	Superoxide dismutase activity, unit per protein mass	SOD/prot	U/mg	Muralisankar, T
7	Superoxide dismutase activity, standard deviation	SOD std dev	±	Muralisankar, T
8	Catalase activity, per protein mass	CAT/prot	µmol/min/mg	Muralisankar, T
9	Catalase activity, standard deviation	CAT std dev	±	Muralisankar, T
10	Lipid peroxidation, per protein	LPO/prot	nmol/mg	Muralisankar, T
11	Lipid peroxidation, standard deviation	LPO std dev	±	Muralisankar, T
12	Glutamic oxaloacetate transaminase activity	GOT act	U/ml	Muralisankar, T
13	Glutamic oxaloacetate transaminase activity, standard deviation	GOT act std dev	±	Muralisankar, T
14	Glutamic pyruvate transaminase activity	GPT act	U/ml	Muralisankar, T
15	Glutamic pyruvate transaminase activity, standard deviation	GPT act std dev	±	Muralisankar, T
16	Hemocyte count	Hemocyte	#	Muralisankar, T
17	Hemocyte count, standard deviation	Hemocyte std dev	±	Muralisankar, T
18	Survival	Survival	%	Muralisankar, T
19	Survival rate, standard deviation	Survival rate std dev	±	Muralisankar, T
20	Length	l	cm	Muralisankar, T		Initial
21	Length, standard deviation	l std dev	±	Muralisankar, T		Initial
22	Length	l	cm	Muralisankar, T		Final
23	Length, standard deviation	l std dev	±	Muralisankar, T		Final
24	Mass	Mass	g	Muralisankar, T		Initial
25	Mass, standard deviation	Mass std dev	±	Muralisankar, T		Initial
26	Mass	Mass	g	Muralisankar, T		Final
27	Mass, standard deviation	Mass std dev	±	Muralisankar, T		Final
28	Change	Change		Muralisankar, T		Length gain (cm)
29	Change, standard deviation	Change std dev	±	Muralisankar, T		Length gain
30	Change	Change		Muralisankar, T		Weight gain (g)
31	Change, standard deviation	Change std dev	±	Muralisankar, T		Weight gain
32	Feed intake	Feed intake	g/day	Muralisankar, T
33	Feed intake, standard deviation	Feed intake std dev	±	Muralisankar, T
34	Specific growth rate	SGR	%/day	Muralisankar, T
35	Specific growth rate, standard deviation	SGR std dev	±	Muralisankar, T
36	Feed conversion ratio	FCR		Muralisankar, T
37	Feed conversion ratio, standard deviation	FCR std dev	±	Muralisankar, T
38	Protein efficiency ratio	PER		Muralisankar, T
39	Protein efficiency ratio, standard deviation	PER std dev	±	Muralisankar, T
40	Molt per day	Molt/day	#/day	Muralisankar, T
41	Molt per day, standard deviation	Molt/day std dev	±	Muralisankar, T
42	Proteins	Protein	g/kg	Muralisankar, T
43	Proteins, standard deviation	Protein std dev	±	Muralisankar, T
44	Amino acids	AA	mg/g	Muralisankar, T
45	Amino acid, standard deviation	AA std dev	±	Muralisankar, T
46	Carbohydrates, total	CHO tot	µg/g	Muralisankar, T
47	Carbohydrates, total, standard deviation	CHO tot std dev	±	Muralisankar, T
48	Lipids, total	Lipids tot	µg/g	Muralisankar, T
49	Lipids, standard deviation	Lipids std dev	±	Muralisankar, T
50	Ash	Ash	%	Muralisankar, T
51	Ash, standard deviation	Ash std dev	±	Muralisankar, T
52	Moisture	Moisture	%	Muralisankar, T
53	Moisture, standard deviation	Moisture std dev	±	Muralisankar, T
54	Chitin	Chitin	g/kg	Muralisankar, T
55	Chitin, standard deviation	Chitin std dev	±	Muralisankar, T
56	Sodium	Na	mg/kg	Muralisankar, T
57	Sodium, standard deviation	Na std dev	±	Muralisankar, T
58	Potassium	K	mg/kg	Muralisankar, T
59	Potassium, standard deviation	K std dev	±	Muralisankar, T
60	Calcium	Ca	mg/kg	Muralisankar, T
61	Calcium, standard deviation	Ca std dev	±	Muralisankar, T
62	Salinity	Sal		Muralisankar, T
63	Salinity, standard deviation	Sal std dev	±	Muralisankar, T
64	Temperature, water	Temp	°C	Muralisankar, T
65	Temperature, water, standard deviation	Temp std dev	±	Muralisankar, T
66	Ammonia	NH3	µg/l	Muralisankar, T
67	Ammonia, standard deviation	NH3 std dev	±	Muralisankar, T
68	Oxygen, dissolved	DO	mg/l	Muralisankar, T
69	Oxygen, dissolved, standard deviation	DO std dev	±	Muralisankar, T
70	pH	pH		Muralisankar, T		total scale
71	pH, standard deviation	pH std dev	±	Muralisankar, T		total scale
72	Alkalinity, total	AT	µmol/kg	Muralisankar, T
73	Alkalinity, total, standard deviation	AT std dev	±	Muralisankar, T
74	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Muralisankar, T	Calculated using CO2calc
75	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Muralisankar, T	Calculated using CO2calc
76	Bicarbonate ion	[HCO3]-	µmol/kg	Muralisankar, T	Calculated using CO2calc
77	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Muralisankar, T	Calculated using CO2calc
78	Carbonate ion	[CO3]2-	µmol/kg	Muralisankar, T	Calculated using CO2calc
79	Carbonate ion, standard deviation	[CO3]2- std dev	±	Muralisankar, T	Calculated using CO2calc
80	Calcite saturation state	Omega Cal		Muralisankar, T	Calculated using CO2calc
81	Calcite saturation state, standard deviation	Omega Cal std dev	±	Muralisankar, T	Calculated using CO2calc
82	Aragonite saturation state	Omega Arg		Muralisankar, T	Calculated using CO2calc
83	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Muralisankar, T	Calculated using CO2calc
84	Carbon, inorganic, dissolved	DIC	µmol/kg	Muralisankar, T	Calculated using CO2calc
85	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Muralisankar, T	Calculated using CO2calc
86	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
87	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
88	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
89	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
90	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
91	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
92	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
93	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
94	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
95	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
96	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
97	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
98	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
99	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
100	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
101	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
102	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

612 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 SOD/prot [U/mg]	7 SOD std dev [±]	8 CAT/prot [µmol/min/mg]	9 CAT std dev [±]	10 LPO/prot [nmol/mg]	11 LPO std dev [±]	12 GOT act [U/ml]	13 GOT act std dev [±]	14 GPT act [U/ml]	15 GPT act std dev [±]	16 Hemocyte [#]	17 Hemocyte std dev [±]	18 Survival [%]	19 Survival rate std dev [±]	20 l [cm] (Initial)	21 l std dev [±] (Initial)	22 l [cm] (Final)	23 l std dev [±] (Final)	24 Mass [g] (Initial)	25 Mass std dev [±] (Initial)	26 Mass [g] (Final)	27 Mass std dev [±] (Final)	28 Change (Length gain (cm))	29 Change std dev [±] (Length gain)	30 Change (Weight gain (g))	31 Change std dev [±] (Weight gain)	32 Feed intake [g/day]	33 Feed intake std dev [±]	34 SGR [%/day]	35 SGR std dev [±]	36 FCR	37 FCR std dev [±]	38 PER	39 PER std dev [±]	40 Molt/day [#/day]	41 Molt/day std dev [±]	42 Protein [g/kg]	43 Protein std dev [±]	44 AA [mg/g]	45 AA std dev [±]	46 CHO tot [µg/g]	47 CHO tot std dev [±]	48 Lipids tot [µg/g]	49 Lipids std dev [±]	50 Ash [%]	51 Ash std dev [±]	52 Moisture [%]	53 Moisture std dev [±]	54 Chitin [g/kg]	55 Chitin std dev [±]	56 Na [mg/kg]	57 Na std dev [±]	58 K [mg/kg]	59 K std dev [±]	60 Ca [mg/kg]	61 Ca std dev [±]	62 Sal	63 Sal std dev [±]	64 Temp [°C]	65 Temp std dev [±]	66 NH3 [µg/l]	67 NH3 std dev [±]	68 DO [mg/l]	69 DO std dev [±]	70 pH (total scale)	71 pH std dev [±] (total scale)	72 AT [µmol/kg]	73 AT std dev [±]	74 pCO2water_SST_wet [µatm] (Calculated using CO2calc)	75 pCO2 std dev [±] (Calculated using CO2calc)	76 [HCO3]- [µmol/kg] (Calculated using CO2calc)	77 [HCO3]- std dev [±] (Calculated using CO2calc)	78 [CO3]2- [µmol/kg] (Calculated using CO2calc)	79 [CO3]2- std dev [±] (Calculated using CO2calc)	80 Omega Cal (Calculated using CO2calc)	81 Omega Cal std dev [±] (Calculated using CO2calc)	82 Omega Arg (Calculated using CO2calc)	83 Omega Arg std dev [±] (Calculated using CO2calc)	84 DIC [µmol/kg] (Calculated using CO2calc)	85 DIC std dev [±] (Calculated using CO2calc)	86 CSC flag (Calculated using seacarb afte...)	87 CO2 [µmol/kg] (Calculated using seacarb afte...)	88 CO2 std dev [±] (Calculated using seacarb afte...)	89 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	90 fCO2 std dev [±] (Calculated using seacarb afte...)	91 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	92 pCO2 std dev [±] (Calculated using seacarb afte...)	93 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	94 [HCO3]- std dev [±] (Calculated using seacarb afte...)	95 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	96 [CO3]2- std dev [±] (Calculated using seacarb afte...)	97 DIC [µmol/kg] (Calculated using seacarb afte...)	98 DIC std dev [±] (Calculated using seacarb afte...)	99 Omega Arg (Calculated using seacarb afte...)	100 Omega Arg std dev [±] (Calculated using seacarb afte...)	101 Omega Cal (Calculated using seacarb afte...)	102 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Litopenaeus vannamei (crustaceans)	247789	marinespecies.org	pH 8.1	0.93	0.01	13.03	2.1	1.77	0.29	21.33	3.4	38.39	1.10	6.37	0.30	93.3	1.50	2.81	0.25	7.66	0.47	0.107	0.03	2.21	0.39	4.85	0.44	2.108	0.39	0.368	0.07	2.65	0.16	0.174	0.03	0.016	0.003	1.13	0.06	217.75	8.90	190.07	4.9	168860	5100	5390	220	21.73	0.44	73	0.810	0.25	0.100	88.00	1.54	70.30	1.60	8.2	1.00	34	0.32	25	0.11	200	10	5.76	0.4	8.22	0.08	1998.2	42	214.42	4.80	1394.14	31.27	239.14	5.36	5.80	0.13	3.81	0.08	1639.38	36.77	8	5.92	1.44	207.31	50.41	207.97	50.57	1384.56	85.91	242.82	32.00	1633.30	62.07	3.88	0.54	5.89	0.82
laboratory	Litopenaeus vannamei (crustaceans)	247789	marinespecies.org	pH 7.8	1.09	0.03	14.76	0.4	2.74	0.53	22.78	5.7	38.61	0.78	5.95	0.01	92.2	1.92	2.81	0.25	6.96	0.61	0.107	0.04	1.63	0.47	4.15	0.58	1.532	0.47	0.362	0.02	2.37	0.25	0.236	0.01	0.015	0.004	1.06	0.02	190.88	6.40	139.80	4.8	161170	5000	4700	90	20.00	0.57	76	0.700	0.25	0.030	76.16	1.32	78.12	1.14	5.6	0.25	34	0.25	25	0.08	200	80	5.76	0.2	7.86	0.06	1998.2	42	658.89	14.27	1705.51	36.95	116.46	2.52	2.82	0.06	1.85	0.04	1840.72	39.88	8	16.40	2.67	574.75	93.73	576.59	94.03	1675.60	52.92	128.28	15.61	1820.28	47.18	2.05	0.27	3.11	0.40
laboratory	Litopenaeus vannamei (crustaceans)	247789	marinespecies.org	pH 7.6	1.28	0.00	16.57	0.3	3.09	0.98	22.97	0.2	39.34	0.74	5.57	0.06	90.0	0.81	2.81	0.25	6.73	0.87	0.107	0.05	1.62	0.63	3.92	0.83	1.451	0.46	0.364	0.05	2.33	0.35	0.250	0.03	0.018	0.005	1.10	0.10	172.49	6.80	100.77	2.3	156690	5400	3800	200	21.00	0.97	75	0.641	0.19	0.020	53.90	1.20	76.50	1.11	4.2	0.12	34	0.20	25	0.12	200	40	5.80	0.7	7.64	0.03	1998.2	42	1104.10	23.67	1803.22	38.66	77.69	1.66	1.88	0.04	1.24	0.02	1912.32	41.00	8	29.09	2.36	1019.42	82.87	1022.69	83.13	1790.80	40.92	82.61	6.03	1902.50	42.17	1.32	0.11	2.01	0.17
laboratory	Litopenaeus vannamei (crustaceans)	247789	marinespecies.org	pH 7.4	1.35	0.00	17.41	0.5	3.50	0.39	25.90	1.6	39.58	1.30	4.95	0.03	90.0	1.20	2.81	0.25	5.73	0.87	0.107	0.06	0.95	0.45	2.92	0.83	0.851	0.45	0.294	0.04	1.85	0.39	0.345	0.05	0.012	0.006	0.90	0.10	158.58	9.20	100.76	3.6	153520	5500	3620	200	19.66	0.74	77	0.410	0.09	0.040	53.60	2.21	60.90	1.21	3.2	0.59	34	0.30	25	0.11	200	20	5.80	0.3	7.46	0.06	1998.2	42	1815.37	38.65	1870.70	39.83	50.85	1.08	1.23	0.02	0.81	0.01	1973.20	42.01	8	45.65	6.87	1599.40	240.88	1604.52	241.65	1856.31	43.77	56.58	7.60	1958.53	45.45	0.90	0.13	1.37	0.19
laboratory	Litopenaeus vannamei (crustaceans)	247789	marinespecies.org	pH 7.2	1.58	0.06	18.12	0.3	4.86	1.62	26.13	2.4	40.24	0.17	4.56	0.33	80.0	1.40	2.81	0.25	4.93	0.96	0.107	0.07	0.80	0.48	2.12	0.92	0.695	0.48	0.246	0.08	1.61	0.57	0.353	0.12	0.013	0.008	0.86	0.09	150.23	7.03	80.30	5.2	126080	4000	3390	100	16.00	0.65	76	0.520	0.03	0.014	40.80	1.10	51.40	1.01	2.4	0.35	34	0.18	25	0.09	200	50	5.72	0.5	7.28	0.07	1998.2	42	2946.67	62.45	1915.89	40.60	32.86	0.69	0.79	0.01	0.52	0.01	2032.59	43.08	8	70.80	12.12	2480.68	424.73	2488.64	426.10	1902.24	43.07	38.30	6.07	2011.34	47.55	0.61	0.10	0.93	0.15
laboratory	Litopenaeus vannamei (crustaceans)	247789	marinespecies.org	pH 7	3.30	0.01	19.18	0.4	5.25	2.07	27.63	2.5	40.70	0.80	4.05	0.18	86.6	1.06	2.81	0.25	4.11	0.87	0.107	0.08	0.49	0.23	1.30	0.83	0.387	0.23	0.178	0.06	1.28	0.39	0.459	0.17	0.007	0.004	0.74	0.12	144.31	3.40	76.22	4.5	96670	5980	3320	100	16.00	0.40	76	2.850	0.02	0.021	34.20	1.14	45.10	1.12	1.7	0.09	34	0.21	25	0.10	200	10	5.76	0.3	7.17	0.20	1998.2	42	4742.43	100.20	1945.54	41.11	21.05	0.44	0.51	0.01	0.33	0.01	2101.52	44.40	8	92.20	44.14	3230.55	1546.55	3240.92	1551.54	1922.96	52.76	30.06	13.37	2045.22	77.49	0.48	0.21	0.73	0.33