Citation:

PANGAEA.928506

Name: Elemental cellular contents of Rhodomonas salina and Oxyrrhis marina, and respiration rate and total lipids of O. marina during a starvation experiment
Published: 2021-02-25
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Dinoflagellata; elemental stoichiometry; Laboratory experiment; N limitation; nutrient; P limitation; Starvation

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Published: 2021-02-25 • DOI registered: 2021-04-08

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Abstract:

This dataset contains data on the cellular elemental contents (carbon, nitrogen and phosphorus) of the phytoplankton species Rhodomonas salina grown in nitrogen limited and phosphorus limited conditions. These two phytoplankton qualities were offered as food to the heterotrophic dinoflagellate Oxyrrhis marina. The resulting cellular elemental contents of the dinoflagellates are also included for a stavation period of up to 78 h, as well as the cellular lipid content and respiration rate of O. marina fed with nitrogen limited or phosphorus limited R. salina, before being starved for 24 h or up to 48 h, respectively. To highlight possible differences in the source of energy used by differently preconditioned O. marina, we determined total lipid content following Urzua and Anger (2011).

Keyword(s):

Dinoflagellata; elemental stoichiometry; Laboratory experiment; N limitation; nutrient; P limitation; Starvation

Related to:

Meunier, Cédric Léo; Haafke, Julia; Oppermann, Bettina; Boersma, Maarten; Malzahn, Arne (2012): Dynamic stoichiometric response to food quality fluctuations in the heterotrophic dinoflagellate Oxyrrhis marina. Marine Biology, 159(10), 2241-2248, https://doi.org/10.1007/s00227-012-2009-3

Further details:

Grasshoff, Klaus; Kremling, Klaus; Ehrhardt, M (1999): Methods of Seawater Analysis, Third Edition. Wiley-VCH, https://doi.org/10.1002/9783527613984.fmatter

Urzúa, Ángel; Anger, Klaus (2011): Larval biomass and chemical composition at hatching in two geographically isolated clades of the shrimp Macrobrachium amazonicum: intra- or interspecific variation? Invertebrate Reproduction & Development, 55(4), 236-246, https://doi.org/10.1080/07924259.2011.576155

Coverage:

Latitude: 54.188557 * Longitude: 7.899978

Event(s):

Strain_B21.89 * Latitude: 54.188557 * Longitude: 7.899978 * Location: Helgoland, North Sea * Method/Device: Laboratory experiment * Comment: Original sampling site of Oxyrrhis marina, strain B21.89 of the Göttingen culture collection, isolated on Helgoland, Germany.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Experimental treatment	Exp treat		Meunier, Cédric Léo
2	Replicate	Repl		Meunier, Cédric Léo
3	Nitrogen per cell	N/cell	pmol/#	Meunier, Cédric Léo	Carbon and nitrogen and sulfur (CNS) isotope element analyzer, Elementar, Vario Micro Cube
4	Carbon per cell	C/cell	pmol/#	Meunier, Cédric Léo	Carbon and nitrogen and sulfur (CNS) isotope element analyzer, Elementar, Vario Micro Cube
5	Phosphorus per cell	P/cell	pmol/#	Meunier, Cédric Léo	Methods of Seawater Analysis, Third Edition (Grasshoff et al., 1999)
6	Respiration rate, oxygen, per cell	Resp O2/cell	pg/#/h	Meunier, Cédric Léo	Fiber-optic oxygen microsensor PSt1 with MicroTX3 transmitter (Presens)
7	Lipids	Lipids	µg/l	Meunier, Cédric Léo	Spectrophotometer, Thermo Fisher Scientific, Multiskan GO Microplate	Urzúa & Anger, 2011

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

794 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Exp treat	2 Repl	3 N/cell [pmol/#]	4 C/cell [pmol/#]	5 P/cell [pmol/#]	6 Resp O2/cell [pg/#/h]	7 Lipids [µg/l]
Oxyrrhis marina 0h -N	1	8.784955256	76.656048211	0.307850273	4.13	426.09
Oxyrrhis marina 0h -N	2	7.775113316	70.375242111	0.331381584	52.08	518.48
Oxyrrhis marina 0h -N	3	8.234063782	78.233727224	0.322977544	12.62	491.30
Oxyrrhis marina 0h -N	4	8.504628921	76.741388342	0.319615928	56.11
Oxyrrhis marina 0h -P	1	11.430114486	83.214527062	0.175066452	23.55	382.61
Oxyrrhis marina 0h -P	2	10.646602938	76.465733389	0.163300797	62.97	366.30
Oxyrrhis marina 0h -P	3	11.649948662	85.723937668	0.183470492	50.23	328.26
Oxyrrhis marina 0h -P	4	10.798795829	76.798420401	0.153215950	43.09	350.00
Oxyrrhis marina 12h -N	1	5.524413335	44.600733737	0.235729362	30.98
Oxyrrhis marina 12h -N	2	5.874476962	44.698767122	0.247879946	52.52
Oxyrrhis marina 12h -N	3	5.659053192	42.862869200	0.230521969	44.75
Oxyrrhis marina 12h -N	4	5.664438786	43.709521154	0.220107183	70.37
Oxyrrhis marina 12h -P	1	7.635566284	49.805415225	0.135053095	44.13
Oxyrrhis marina 12h -P	2	7.549396776	48.432947846	0.141996286	18.04
Oxyrrhis marina 12h -P	3	7.624795096	47.131777474	0.141996286	31.93
Oxyrrhis marina 12h -P	4	7.161633989	45.456297817	0.124638309	44.77
Oxyrrhis marina 1h -N	1	8.865382439	80.733632487	0.292723003	21.32
Oxyrrhis marina 1h -N	2	8.560996658	79.935183658	0.296084618	46.73
Oxyrrhis marina 1h -N	3	8.335525709	77.074075354	0.296084618	51.94
Oxyrrhis marina 1h -N	4	9.440333360	82.805797305	0.311211889	63.34
Oxyrrhis marina 1h -P	1	10.432405536	75.153996027	0.155882668	39.19
Oxyrrhis marina 1h -P	2	9.767266236	69.992594668	0.154146870	51.04
Oxyrrhis marina 1h -P	3	10.037831375	69.678918343	0.155882668	44.80
Oxyrrhis marina 1h -P	4	10.505683595	75.600747157		31.15
Oxyrrhis marina 24h -N	1	6.170684646	41.757765596	0.254823137	17.52	259.65
Oxyrrhis marina 24h -N	2	5.955260876	40.055549562	0.265237923	21.60	354.41
Oxyrrhis marina 24h -N	3	5.982188847	40.795256006	0.247879946	21.97	245.83
Oxyrrhis marina 24h -N	4	5.971417659	40.242704204	0.249615744	25.91	399.81
Oxyrrhis marina 24h -P	1	6.197612617	37.854254481	0.117695118	23.85	279.31
Oxyrrhis marina 24h -P	2	6.283782125	39.957516178	0.114223523	14.62	292.92
Oxyrrhis marina 24h -P	3	5.799078642	37.221493547	0.099430098	16.32	367.88
Oxyrrhis marina 24h -P	4	6.192227023	38.950445958	0.097749290	22.69	386.16
Oxyrrhis marina 2h -N	1	8.769557286	79.707055421	0.334669831	84.17
Oxyrrhis marina 2h -N	2	7.839489621	71.827125906		54.52
Oxyrrhis marina 2h -N	3	7.856399942	72.482994587	0.305161270	59.59
Oxyrrhis marina 2h -N	4	8.172059271	75.163501370	0.331198236	45.44
Oxyrrhis marina 2h -P	1	9.812360426	70.068637414	0.154146870	36.62
Oxyrrhis marina 2h -P	2	9.716535273	70.135174816	0.155882668	45.97
Oxyrrhis marina 2h -P	3	9.975826864	71.408890805	0.159354263	12.38
Oxyrrhis marina 2h -P	4	10.139293302	71.893663309	0.159354263	28.08
Oxyrrhis marina 3h -N	1	7.766211562	72.178823605	0.287803293	25.73
Oxyrrhis marina 3h -N	2	8.234063782	73.224411357	0.296482282	63.07
Oxyrrhis marina 3h -N	3	7.215489932	0.000000000	0.280860102	47.32
Oxyrrhis marina 3h -N	4	7.910231591	76.203131946	0.298218079	51.79
Oxyrrhis marina 3h -P	1	9.138147082	65.927450859	0.136788893	6.64
Oxyrrhis marina 3h -P	2	8.998121631	64.804523004	0.162825859	24.66
Oxyrrhis marina 3h -P	3	9.617464971	75.151501097		16.89
Oxyrrhis marina 3h -P	4	9.337414070	70.062677246	0.162825859	17.95
Oxyrrhis marina 48h -N	1	5.609115893	34.662213804	0.213725033	24.44
Oxyrrhis marina 48h -N	2	5.136215387	34.817629242	0.215405841	2.25
Oxyrrhis marina 48h -N	3	5.050233477	34.479372112	0.217086649	13.77
Oxyrrhis marina 48h -N	4		36.774035349	0.240617959	12.59
Oxyrrhis marina 48h -P	1	6.264727958	38.236768887	0.087664443	9.44
Oxyrrhis marina 48h -P	2	5.168458603	33.089775251	0.087664443	3.94
Oxyrrhis marina 48h -P	3	5.770331974	33.848568273	0.089345251	13.57
Oxyrrhis marina 48h -P	4	5.996034489	36.618619911		7.55
Oxyrrhis marina 6h -N	1	6.601532187	55.348757493	0.277388507	36.40
Oxyrrhis marina 6h -N	2	6.310710097	52.889010763	0.254823137	51.15
Oxyrrhis marina 6h -N	3	6.359180445	52.184952822	0.256558935	33.38
Oxyrrhis marina 6h -N	4	6.256854154	51.275915987	0.232257767	56.95
Oxyrrhis marina 6h -P	1	8.529574931	57.193567541	0.150675275	21.64
Oxyrrhis marina 6h -P	2	7.511697616	52.372107465	0.133317298	47.50
Oxyrrhis marina 6h -P	3	8.497261365	58.566034920	0.148939477	37.46
Oxyrrhis marina 6h -P	4	7.974858722	55.428966626	0.135053095	14.08
Oxyrrhis marina 78h -N	1	5.394161117	31.444200021	0.213725033
Oxyrrhis marina 78h -N	2	6.049773182	34.991328850	0.235485916
Oxyrrhis marina 78h -N	3	5.711219411	33.720579089	0.220333380
Oxyrrhis marina 78h -N	4	5.383413378	32.586960597	0.220333380
Oxyrrhis marina 78h -P	1	5.555377199	33.711437004	0.078909715
Oxyrrhis marina 78h -P	2	6.248606350	30.904817029	0.075542485
Oxyrrhis marina 78h -P	3	5.146963125	30.264871106	0.080593330
Oxyrrhis marina 78h -P	4	5.050233477	30.027176906	0.070491640
Oxyrrhis marina constantly fed with Rhodomonas salina -N	1	6.560062852	54.654915473	0.260787653
Oxyrrhis marina constantly fed with Rhodomonas salina -N	2	6.484459712	49.399547103	0.257426038
Oxyrrhis marina constantly fed with Rhodomonas salina -N	3	6.781472048	52.787153892	0.282638155
Oxyrrhis marina constantly fed with Rhodomonas salina -N	4	6.360254554	50.965170781	0.264149269
Oxyrrhis marina constantly fed with Rhodomonas salina -P	1	10.669633531	72.609231453	0.124642216
Oxyrrhis marina constantly fed with Rhodomonas salina -P	2	10.307818504	68.892019679	0.116238177
Oxyrrhis marina constantly fed with Rhodomonas salina -P	3	11.204255735	73.030393378	0.126323024
Oxyrrhis marina constantly fed with Rhodomonas salina -P	4	11.112451922	70.649912932	0.159939181
Oxyrrhis marina preconditioned on Rhodomonas salina -N	1	9.130310805	87.235287237	0.314332020
Oxyrrhis marina preconditioned on Rhodomonas salina -N	2	8.989391461	86.180194141	0.381480364
Oxyrrhis marina preconditioned on Rhodomonas salina -N	3	8.972481140	85.657400265	0.295866225
Oxyrrhis marina preconditioned on Rhodomonas salina -N	4	9.316324338	86.503375810	0.322725563
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 10 cells of Rhodomonas salina -P per Oxyrrhis cell	1	7.338634666	48.860266397	0.198501126
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 10 cells of Rhodomonas salina -P per Oxyrrhis cell	2	7.381905683	49.931301745	0.171641789
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 10 cells of Rhodomonas salina -P per Oxyrrhis cell	3	7.376496806	50.453088196	0.178356623
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 10 cells of Rhodomonas salina -P per Oxyrrhis cell	4	7.176368352	49.720756334	0.186750166
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 100 cells of Rhodomonas salina -P per Oxyrrhis cell	1	7.532103223	48.319175208	0.101110906
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 100 cells of Rhodomonas salina -P per Oxyrrhis cell	2	6.959679449	43.613148480	0.121280601
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 100 cells of Rhodomonas salina -P per Oxyrrhis cell	3	8.028923857	51.935674348	0.117918985
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 100 cells of Rhodomonas salina -P per Oxyrrhis cell	4	7.953320717	49.060786424	0.121280601
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 20 cells of Rhodomonas salina -P per Oxyrrhis cell	1	9.783447125	65.960215367	0.191786292
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 20 cells of Rhodomonas salina -P per Oxyrrhis cell	2	9.253377167	64.468089199	0.183392749
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 20 cells of Rhodomonas salina -P per Oxyrrhis cell	3	9.897033544	68.029052876	0.181714040
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 20 cells of Rhodomonas salina -P per Oxyrrhis cell	4	9.226332781	63.552674372	0.227039173
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 30 cells of Rhodomonas salina -P per Oxyrrhis cell	1	11.281706088	79.435121622	0.218645630
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 30 cells of Rhodomonas salina -P per Oxyrrhis cell	2	12.731285156	87.106297873	0.238790133
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 30 cells of Rhodomonas salina -P per Oxyrrhis cell	3	11.828002677	83.188322413	0.218645630
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 30 cells of Rhodomonas salina -P per Oxyrrhis cell	4	12.087628779	83.728417161	0.213609504
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 5 cells of Rhodomonas salina -P per Oxyrrhis cell	1	7.673985047	46.040788729	0.208573378
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 5 cells of Rhodomonas salina -P per Oxyrrhis cell	2	6.100001805	42.873453428	0.169963080
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 5 cells of Rhodomonas salina -P per Oxyrrhis cell	3	6.646298394	45.253531978	0.180035332
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 5 cells of Rhodomonas salina -P per Oxyrrhis cell	4	6.246041487	43.074844689	0.200179835
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 50 cells of Rhodomonas salina -P per Oxyrrhis cell	1	8.655349874	56.028269576	0.129674074
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 50 cells of Rhodomonas salina -P per Oxyrrhis cell	2	9.400580824	59.544056081	0.133046256
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 50 cells of Rhodomonas salina -P per Oxyrrhis cell	3	8.304335295	51.230685908	0.136407871
Oxyrrhis marina preconditioned on Rhodomonas salina -N fed with 50 cells of Rhodomonas salina -P per Oxyrrhis cell	4	8.439340902	50.855302453	0.133046256
Oxyrrhis marina preconditioned on Rhodomonas salina -P	1	12.072706692	87.377867385	0.159890828
Oxyrrhis marina preconditioned on Rhodomonas salina -P	2	12.315087962	87.121223118	0.151497285
Oxyrrhis marina preconditioned on Rhodomonas salina -P	3	12.033540007	85.110693550	0.158212120
Oxyrrhis marina preconditioned on Rhodomonas salina -P	4	12.206624075	83.966425016	0.164926954
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 10 cells of Rhodomonas salina -N per Oxyrrhis cell	1	6.619254009	42.873453428	0.149818577
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 10 cells of Rhodomonas salina -N per Oxyrrhis cell	2	6.895106742	44.576125006	0.149818577
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 10 cells of Rhodomonas salina -N per Oxyrrhis cell	3	7.398132314	45.161990495	0.158212120
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 10 cells of Rhodomonas salina -N per Oxyrrhis cell	4	6.938377759	43.166386172	0.151497285
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 100 cells of Rhodomonas salina -N per Oxyrrhis cell	1	7.213489990	46.249988359	0.296084618
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 100 cells of Rhodomonas salina -N per Oxyrrhis cell	2	6.970479898	46.616216120	0.289361387
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 100 cells of Rhodomonas salina -N per Oxyrrhis cell	3	6.830074066	46.332389605	0.291042195
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 100 cells of Rhodomonas salina -N per Oxyrrhis cell	4	6.732870029	45.233706323	0.289361387
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 20 cells of Rhodomonas salina -N per Oxyrrhis cell	1	8.047197569	56.513134351	0.208573378
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 20 cells of Rhodomonas salina -N per Oxyrrhis cell	2	8.463681107	60.110714622	0.255577219
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 20 cells of Rhodomonas salina -N per Oxyrrhis cell	3	7.349452420	51.963522661	0.196822418
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 20 cells of Rhodomonas salina -N per Oxyrrhis cell	4	7.976882166	55.616027821	0.258934636
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 30 cells of Rhodomonas salina -N per Oxyrrhis cell	1	10.928844296	61.997782079	0.285793974
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 30 cells of Rhodomonas salina -N per Oxyrrhis cell	2	13.461549484	85.106753795	0.415054536
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 30 cells of Rhodomonas salina -N per Oxyrrhis cell	3	13.601955316	90.957242275	0.359657152
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 30 cells of Rhodomonas salina -N per Oxyrrhis cell	4	9.708393609	55.240879890	0.279079139
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 5 cells of Rhodomonas salina -N per Oxyrrhis cell	1	6.240632610	41.189090146	0.148139868
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 5 cells of Rhodomonas salina -N per Oxyrrhis cell	2	5.959371000	39.614576643	0.131352782
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 5 cells of Rhodomonas salina -N per Oxyrrhis cell	3	6.678751657	39.156869229	0.131352782
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 5 cells of Rhodomonas salina -N per Oxyrrhis cell	4	5.997233140	39.367414640	0.156533411
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 50 cells of Rhodomonas salina -N per Oxyrrhis cell	1	6.727469805	44.592807741	0.257426038
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 50 cells of Rhodomonas salina -N per Oxyrrhis cell	2	7.667108830	46.579593344	0.257426038
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 50 cells of Rhodomonas salina -N per Oxyrrhis cell	3	7.391697392	45.837982128	0.232213920
Oxyrrhis marina preconditioned on Rhodomonas salina -P fed with 50 cells of Rhodomonas salina -N per Oxyrrhis cell	4	7.143287075	48.035348694	0.265830077
Rhodomonas salina -N	1	0.527374158	7.317721302	0.019091158
Rhodomonas salina -N	2	0.485408439	6.462912628	0.018919937
Rhodomonas salina -N	3	0.440786662	6.086003318	0.034672283
Rhodomonas salina -N	4	0.443442720	6.811869333	0.016694062
Rhodomonas salina -P	1	0.661770700	4.667634073	0.004879803
Rhodomonas salina -P	2	0.575183204	3.700113285	0.004879803
Rhodomonas salina -P	3	0.703205207	5.232998038	0.005051024
Rhodomonas salina -P	4	0.693643398	4.739769826	0.004194918