Perner, Mirjam; Kuever, Jan; Seifert, Richard; Pape, Thomas; Koschinsky, Andrea; Schmidt, Katja; Strauss, Harald; Imhoff, Johannes F (2021): Physico-chemical parameters and accession numbers from the Logatchev Hydrothermal Field, located 15°N on the Mid-Atlantic Ridge [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.928270
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Published: 2021-02-19 • DOI registered: 2021-05-06
Abstract:
Hydrothermal fluid samples were retrieved during dives made by the remotely operated vehicle (ROV) QUEST (MARUM, University of Bremen) during the HYDROMAR I (M60/3, 2004) and HYDROMAR II (M64/2, 2005) cruises to the Logatchev hyrothermal field. The samples were retrieved using a pumped flow-through system (Kiel Pumping System KIPS) specially designed for the ROV QUEST. Once on board the ship, the liquids intended for microbiological studies were concentrated on 0.2 µm pore size polycarbonate filters (Sartorius) and stored at -20°C. The pH and sulfide concentrations were determined immediately after sample recovery. The pH was measured (Mettler electrodes with Ag/AgCl reference electrode) at 25°C in unfiltered sample aliquots. Sulfide concentrations were determined photometrically following the methylene blue method or, for samples with low concentrations, by voltammetry (Metrohm Application Bulletin 199/3e). Methane was analyzed on board by applying a purge and trap technique. For on-board measurements of dissolved hydrogen the water sample was degassed into a high-grade vacuum. Aliquots of the released gas were analyzed by gas chromatography (Thermo Electron Corporation Trace GC Ultra with a pulsed discharge detector). The abundance of bacterial and archaeal taxa was investigated by sequencing of the 16S rRNA gene. The diversity of the cbbL, cbbM and aclb was investigated by sequencing of the genes.
Keyword(s):
Supplement to:
Perner, Mirjam; Kuever, Jan; Seifert, Richard; Pape, Thomas; Koschinsky, Andrea; Schmidt, Katja; Strauss, Harald; Imhoff, Johannes F (2007): The influence of ultramafic rocks on microbial communities at the Logatchev hydrothermal field, located 15°N on the Mid-Atlantic Ridge. FEMS Microbiology Ecology, 61(1), 97-109, https://doi.org/10.1111/j.1574-6941.2007.00325.x
Coverage:
Latitude: 14.750000 * Longitude: -44.966670
Minimum Elevation: -3060.0 m * Maximum Elevation: -2960.0 m
Event(s):
Comment:
Gene sequences obtained in this study were submitted to DDBJ/EMBL/GenBank database and assigned the accession numbers.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Area/locality | Area | Perner, Mirjam | |||
| 2 | Temperature, water | Temp | °C | Perner, Mirjam | single value or range min | |
| 3 | Temperature, water | Temp | °C | Perner, Mirjam | range max | |
| 4 | pH | pH | Perner, Mirjam | 25°C | ||
| 5 | Hydrogen sulfide | H2S | µmol/kg | Perner, Mirjam | ||
| 6 | Hydrogen | H2 | mmol/kg | Perner, Mirjam | ||
| 7 | Methane | CH4 | mmol/kg | Perner, Mirjam | ||
| 8 | Gene name | Gene | Perner, Mirjam | |||
| 9 | Accession number, genetics | Access no gen | Perner, Mirjam | range | ||
| 10 | Accession number, genetics | Access no gen | Perner, Mirjam | range |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
26 data points