Citation:

PANGAEA.927579

Name: Oxygen consumption (resting and after treatment), immune variables and hormone levels of Rhinella jimi (anura:bufonidae) collected in 3 different periods from Caatinga, Brazil
Creator: Carla Bonetti Madelaire
Published: 2021-02-08
License: https://creativecommons.org/licenses/by/4.0/
Keywords: bacterial killing ability; body condition index; corticosterone; Metabolic rate; PHA swelling response; testosterone

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Published: 2021-02-08 • DOI registered: 2021-07-06

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Abstract:

To understand the possible interactions between steroid hormones (androgens [AN] and corticosterone [CORT]), metabolism, and immunity during fluctuations of environmental resource, we studied the interactions between field steroid plasma levels (AN and CORT), field immune parameters (plasma bacterial killing ability [BKA], swelling response to phytohemagglutinin (PHA - 20 ug/ml) injection), resting metabolic rate (RMR), rates of oxygen consumption after PHA injection, and parasite load in 3 periods A) within the rainy season (N = 25 in March 21 to April 15, 2013); B) during a breeding event (N = 5; February 15 to 18, 2014); and C) during the dry season (N = 8 collected in September 2 to 14, 2013) of a toad (Rhinella jimi) from a highly seasonal environment (the Brazilian semi-arid, Caatinga, 5°30'43''S. 36°36'18''W).

Considering that air temperature also can affect metabolism and immune response, we also provide the air temperature registered during each oxygen consumption measure and during the development of the swelling response to the PHA injection.

The rates of oxygen consumption (RMR and after treatment) were measured using positive-pressure, flow-through respirometry (Withers, 1977). The air was pumped through the chambers at 100 mL.min^-1^ using a Flowbar Multichannel – 8 pump system (Sable Systems; Henderson, NV, USA).

RMR consisted of lowest value of oxygen consumption for whole 5 minutes. Oxygen consumption after the treatment is the highest value ~ after PHA (V,O2PHA) and saline (V,O2Saline) injection. The metabolic cost to PHA (MC~PHA~) and saline (MCsaline) injections were calculated by subtracting the RMR from their respective V,O2PHA and V,O2saline.

Circulating plasma levels of AN and CORT were quantified using enzyme immunoassay kits (Testosterone ELISA Kit 582701; Corticosterone ELISA Kit 500655) from Cayman Chemical (Ann Arbor, MI).

Keyword(s):

bacterial killing ability; body condition index; corticosterone; Metabolic rate; PHA swelling response; testosterone

Coverage:

Latitude: -5.511940 * Longitude: -36.605000

Date/Time Start: 2013-03-21T00:00:00 * Date/Time End: 2013-09-14T00:00:00

Event(s):

Rhinella_jimi_experiment * Latitude: -5.511940 * Longitude: -36.605000 * Date/Time Start: 2013-03-21T00:00:00 * Date/Time End: 2013-09-14T00:00:00 * Location: Caatinga, Brazil * Method/Device: Field experiment

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Individual code	Individual code		Madelaire, Carla Bonetti
2	Period	Period		Madelaire, Carla Bonetti
3	Oxygen consumption	O2 con	ml/g/h	Madelaire, Carla Bonetti	Respirometer (RESP)	after treatment
4	Temperature, air	TTT	°C	Madelaire, Carla Bonetti		after treatment
5	Metabolic rate of oxygen	MR O2	ml/g/h	Madelaire, Carla Bonetti		Metabolic cost to the treatment
6	Experimental treatment	Exp treat		Madelaire, Carla Bonetti
7	Testosterone, plasma	C19H28O2 plasma	ng/ml	Madelaire, Carla Bonetti	Testosterone enzyme immunoassays (Cayman Chemical)	Testosterone ELISA Kit 582701
8	Corticosterone, plasma	C21H30O4 plasma	ng/ml	Madelaire, Carla Bonetti	Corticosterone enzyme immunoassays (Cayman Chemical)	Corticosterone ELISA Kit 500655
9	Body condition index	BCI		Madelaire, Carla Bonetti
10	Fat body index, abdominal	F B index abdomi		Madelaire, Carla Bonetti
11	Helminth, total	Helminth tot	#	Madelaire, Carla Bonetti
12	Swelling response	Swell resp	%	Madelaire, Carla Bonetti		to PHA
13	Temperature, air	TTT	°C	Madelaire, Carla Bonetti		during max PHA swelling response
14	Bakterial killing ability	BKA	%	Madelaire, Carla Bonetti
15	Oxygen consumption, resting	O2 con rest	ml/g/h	Madelaire, Carla Bonetti	Respirometer (RESP)
16	Temperature, air	TTT	°C	Madelaire, Carla Bonetti		during resting oxigen consumption

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

475 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Individual code	2 Period	3 O2 con [ml/g/h] (after treatment, Respirometer)	4 TTT [°C] (after treatment)	5 MR O2 [ml/g/h] (Metabolic cost to the treatment)	6 Exp treat	7 C19H28O2 plasma [ng/ml] (Testosterone ELISA Kit 582701...)	8 C21H30O4 plasma [ng/ml] (Corticosterone ELISA Kit 5006...)	9 BCI	10 F B index abdomi	11 Helminth tot [#]	12 Swell resp [%] (to PHA)	13 TTT [°C] (during max PHA swelling response)	14 BKA [%]	15 O2 con rest [ml/g/h] (Respirometer)	16 TTT [°C] (during resting oxigen consump...)
IND36	Breeding					65.69	22.80	0.3960	0.865	42	5.74		90.000
IND37	Breeding					21.87	22.63	-1.5100	-0.489	40	15.80		0.000
IND38	Breeding					4.12	12.77	0.3290	0.563	35	13.63		0.000
IND39	Breeding					21.60	10.04	0.1210	-1.262	298	12.51		93.330
IND40	Breeding					20.41	5.97	0.6640	0.323	29	4.98		90.000
IND1	Reproductive Period	0.243	31.300	0.157	PHA	8.75	0.58	1.3370	0.757	68			72.973	0.086	32.020
IND2	Reproductive Period	0.406	30.380	0.337	Saline	17.49	0.26	-0.9820	0.784	23			72.973	0.069	31.410
IND4	Reproductive Period	0.135	31.760	0.108	Saline	15.81	0.91	-0.2440		13			70.270	0.027	29.000
IND5	Reproductive Period	0.268	28.270	0.234	Saline	5.90	0.87	1.0450	0.281	12			62.162	0.034	27.500
IND6	Reproductive Period	0.186	27.910		PHA	77.62	0.39	-1.4940	1.406	80			37.838
IND7	Reproductive Period	0.273	31.290	0.251	PHA	51.62	2.56	-0.6510	-1.555	46			48.649	0.022	28.600
IND8	Reproductive Period	0.273	34.730	0.249	PHA	51.78	4.12	-0.7010	-1.833	23			56.757	0.024	28.600
IND9	Reproductive Period	0.058	28.230	0.030	PHA	60.89	1.43	0.0201	-1.590	42			67.568	0.028	32.760
IND10	Reproductive Period	0.087	28.160	0.069	Saline	87.97	9.44	0.6357	-0.735	62			81.081	0.018	30.210
IND11	Reproductive Period	0.266	35.130	0.221	PHA	58.45	0.71	-0.1380	-0.228	16	3.58	32.6	56.757	0.044	28.300
IND12	Reproductive Period	0.315	33.450	0.246	Saline	5.01	1.49	-0.6280	0.052	8			62.162	0.069	30.500
IND13	Reproductive Period	0.314	33.910		PHA	18.21	0.67	-1.5810	0.566	25	7.14	32.6	56.757
IND14	Reproductive Period	0.352	29.500	0.284	Saline	47.21	1.80	-0.4000	0.517	18			56.757	0.068	30.100
IND15	Reproductive Period	0.283	33.170		PHA	15.12	0.29	0.4760	1.378	26	6.52	29.1	51.351
IND16	Reproductive Period	0.457	29.300		PHA	14.45	0.46	0.6370	-0.601	34	4.01	29.1	54.054
IND17	Reproductive Period	0.442	29.300	0.353	Saline	9.13	1.62	2.0050	0.619	23			10.811	0.090	29.600
IND18	Reproductive Period	0.295	32.800		Saline	7.29	1.11	1.9170	0.250	34			0.000
IND19	Reproductive Period	0.206	34.010	0.115	PHA	2.72	0.30	0.1200	-0.405	49	3.62	28.9	75.676	0.092	30.370
IND20	Reproductive Period	0.326	34.640	0.202	PHA	8.36	0.54	-0.1770	-1.272	13	3.89	28.9	78.378	0.124	30.370
IND21	Reproductive Period	0.205	35.030	0.133	Saline	4.41	1.50	-1.0090	0.510	178			48.649	0.072	29.600
IND23	Reproductive Period	0.208	33.660	0.186	Saline	1.85	1.42	0.0838	-0.411	46			45.946	0.022	28.540
IND24	Reproductive Period	0.258	35.030	0.194	PHA			-0.2710	1.042	15	7.21	30.0	62.162	0.064	28.540
IND25	Reproductive Period	0.414	29.030	0.340	PHA	3.50	2.27				8.02	30.0	0.000	0.073	28.540
IND26	Dryperiod	0.230	29.835	0.146	PHA	0.88	0.87	0.4320		50	9.90	27.3	0.000	0.089	27.847
IND28	Dryperiod	0.180	33.380	0.082	Saline	1.66	2.36	0.8160		10			86.490	0.027	25.560
IND29	Dryperiod	0.110	26.730	0.177	PHA	2.72	0.92	-0.0630		24	5.00	27.7	66.670	0.033	26.113
IND30	Dryperiod	0.070	28.143	0.046	Saline	14.67	1.69	0.9800		39			45.050	0.028	30.510
IND31	Dryperiod	0.070	25.820	0.175	PHA	14.11	1.76	0.2390		38	6.70	28.1	28.830	0.065	28.833
IND33	Dryperiod	0.210	30.239	0.105	PHA	1.93	0.61	-1.9260		6	5.20	27.9	0.000	0.077	26.405
IND34	Dryperiod	0.240	28.143	0.090	PHA	1.17		0.1500		80	1.60	25.7	90.990	0.031	25.170
IND35	Dryperiod	0.120	27.585	0.029	Saline	16.31	1.37	-0.6270		231			90.990	0.044	26.990