Madelaire, Carla Bonetti (2021): Oxygen consumption (resting and after treatment), immune variables and hormone levels of Rhinella jimi (anura:bufonidae) collected in 3 different periods from Caatinga, Brazil [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.927579
Always quote citation above when using data! You can download the citation in several formats below.
Published: 2021-02-08 • DOI registered: 2021-07-06
Abstract:
To understand the possible interactions between steroid hormones (androgens [AN] and corticosterone [CORT]), metabolism, and immunity during fluctuations of environmental resource, we studied the interactions between field steroid plasma levels (AN and CORT), field immune parameters (plasma bacterial killing ability [BKA], swelling response to phytohemagglutinin (PHA - 20 ug/ml) injection), resting metabolic rate (RMR), rates of oxygen consumption after PHA injection, and parasite load in 3 periods A) within the rainy season (N = 25 in March 21 to April 15, 2013); B) during a breeding event (N = 5; February 15 to 18, 2014); and C) during the dry season (N = 8 collected in September 2 to 14, 2013) of a toad (Rhinella jimi) from a highly seasonal environment (the Brazilian semi-arid, Caatinga, 5°30'43''S. 36°36'18''W).
Considering that air temperature also can affect metabolism and immune response, we also provide the air temperature registered during each oxygen consumption measure and during the development of the swelling response to the PHA injection.
The rates of oxygen consumption (RMR and after treatment) were measured using positive-pressure, flow-through respirometry (Withers, 1977). The air was pumped through the chambers at 100 mL.min^-1^ using a Flowbar Multichannel – 8 pump system (Sable Systems; Henderson, NV, USA).
RMR consisted of lowest value of oxygen consumption for whole 5 minutes. Oxygen consumption after the treatment is the highest value ~ after PHA (V,O2PHA) and saline (V,O2Saline) injection. The metabolic cost to PHA (MC~PHA~) and saline (MCsaline) injections were calculated by subtracting the RMR from their respective V,O2PHA and V,O2saline.
Circulating plasma levels of AN and CORT were quantified using enzyme immunoassay kits (Testosterone ELISA Kit 582701; Corticosterone ELISA Kit 500655) from Cayman Chemical (Ann Arbor, MI).
Keyword(s):
Coverage:
Latitude: -5.511940 * Longitude: -36.605000
Date/Time Start: 2013-03-21T00:00:00 * Date/Time End: 2013-09-14T00:00:00
Event(s):
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Individual code | Individual code | Madelaire, Carla Bonetti | |||
| 2 | Period | Period | Madelaire, Carla Bonetti | |||
| 3 | Oxygen consumption | O2 con | ml/g/h | Madelaire, Carla Bonetti | Respirometer (RESP) | after treatment |
| 4 | Temperature, air | TTT | °C | Madelaire, Carla Bonetti | after treatment | |
| 5 | Metabolic rate of oxygen | MR O2 | ml/g/h | Madelaire, Carla Bonetti | Metabolic cost to the treatment | |
| 6 | Experimental treatment | Exp treat | Madelaire, Carla Bonetti | |||
| 7 | Testosterone, plasma | C19H28O2 plasma | ng/ml | Madelaire, Carla Bonetti | Testosterone enzyme immunoassays (Cayman Chemical) | Testosterone ELISA Kit 582701 |
| 8 | Corticosterone, plasma | C21H30O4 plasma | ng/ml | Madelaire, Carla Bonetti | Corticosterone enzyme immunoassays (Cayman Chemical) | Corticosterone ELISA Kit 500655 |
| 9 | Body condition index | BCI | Madelaire, Carla Bonetti | |||
| 10 | Fat body index, abdominal | F B index abdomi | Madelaire, Carla Bonetti | |||
| 11 | Helminth, total | Helminth tot | # | Madelaire, Carla Bonetti | ||
| 12 | Swelling response | Swell resp | % | Madelaire, Carla Bonetti | to PHA | |
| 13 | Temperature, air | TTT | °C | Madelaire, Carla Bonetti | during max PHA swelling response | |
| 14 | Bakterial killing ability | BKA | % | Madelaire, Carla Bonetti | ||
| 15 | Oxygen consumption, resting | O2 con rest | ml/g/h | Madelaire, Carla Bonetti | Respirometer (RESP) | |
| 16 | Temperature, air | TTT | °C | Madelaire, Carla Bonetti | during resting oxigen consumption |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
475 data points