Visser, Fleur; Merten, Veronique; Bayer, Till; Oudejans, Machiel G; de Jonge, Danielle; Puebla, Oscar; Reusch, Thorsten B H; Fuss, Janina; Hoving, Henk-Jan T (2021): Environmental DNA analysis of cephalopods off the Azores [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.926840
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Abstract:
Fundamental insight on predator-prey dynamics in the deep sea is hampered by a lack of combined data on hunting behavior and prey spectra. Deep-sea niche segregation may evolve when predators target specific prey communities, but this hypothesis remains untested. We combined environmental DNA (eDNA) metabarcoding with biologging to assess cephalopod community composition in the deep-sea foraging habitat of two top predator cetaceans. Here, we are presenting the eDNA data from seawater samples obtained during a cruise on RV Pelagia in 2018 off Terceira, Azores, sampled directly in the foraging habitats of two cetacean top-predators from the surface to 1600 m. The water was collected using Niskin bottles mounted on a CTD rosette at seven or eight depths in biological triplicates and filtered on sterile Sterivex filter. After DNA extraction and PCR amplification with two universal cephalopod primer (Ceph18S, targeting the nuclear 18S rRNA gene and CephMLS targeting the mitochondrial 16S rRNA gene), the samples were sequenced on an Illumina MiSeq with the MiSeq Reagent kit v3 (600 cycles).
Keyword(s):
Supplement to:
Visser, Fleur; Merten, Veronique; Bayer, Till; Oudejans, Machiel G; de Jonge, Danielle; Puebla, Oscar; Reusch, Thorsten B H; Fuss, Janina; Hoving, Henk-Jan T (2021): Deep-sea predator niche segregation revealed by combined cetacean biologging and eDNA analysis of cephalopod prey. Science Advances, 7(14), eabf5908, https://doi.org/10.1126/sciadv.abf5908
Coverage:
Median Latitude: 38.641397 * Median Longitude: -27.365617 * South-bound Latitude: 38.597990 * West-bound Longitude: -27.445000 * North-bound Latitude: 38.690410 * East-bound Longitude: -27.266200
Date/Time Start: 2018-07-23T00:00:00 * Date/Time End: 2018-07-24T06:00:00
Event(s):
64PE_NICO_Leg12_1 * Latitude: 38.597990 * Longitude: -27.266200 * Date/Time: 2018-07-23T17:00:00 * Location: South Atlantic Ocean * Campaign: 64PE_NICO_Leg12 * Basis: Pelagia * Method/Device: CTD/Rosette (CTD-RO)
64PE_NICO_Leg12_2 * Latitude: 38.635710 * Longitude: -27.313300 * Date/Time: 2018-07-23T00:00:00 * Location: South Atlantic Ocean * Campaign: 64PE_NICO_Leg12 * Basis: Pelagia * Method/Device: CTD/Rosette (CTD-RO)
64PE_NICO_Leg12_3 * Latitude: 38.656380 * Longitude: -27.359900 * Date/Time: 2018-07-23T08:00:00 * Location: South Atlantic Ocean * Campaign: 64PE_NICO_Leg12 * Basis: Pelagia * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | File content | Content | Merten, Veronique | |||
| 2 | Binary Object | Binary | Merten, Veronique |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
10 data points
Data
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| 1 Content | 2 Binary |
|---|---|
| Sequencing results of seawater samples taken off the Azores being amplified with the universal cephalopod primer Ceph18S using IDTAXA | eDNA_Azores_sequence_variants_Ceph18S.fasta |
| Sequencing results of seawater samples taken off the Azores being amplified with the universal cephalopod primer CephMLS using IDTAXA | eDNA_Azores_sequence_variants_CephMLS.fasta |
| Sequencing results combined with species allignments and environmental data for both universal cephalopod primer pairs CephMLS and Ceph18S | eDNA_Azores_species_alignments.csv |
| Reference library of the mt 16S rRNA gene for cephalopods used for the identification of sequence variants with IDTAXA | eDNA_Azores_trainingset_CephMLS_species_alignment.RDS |
| Reference library of the nuclear 18S rRNA gene for cephalopods used for the identification of sequence variants with IDTAXA | eDNA_Azores_trainingset_for_species_allignment_Ceph18S.RDS |