Citation:

PANGAEA.926819

Name: Seawater carbonate chemistry and metabolic rates, bloodbased metrics in newborn blacktip reef sharks
Published: 2020
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Carcharhinus melanopterus; Chordata; Coast and continental shelf; Laboratory experiment; Mesocosm or benthocosm; Nekton; Other studied parameter or process; Pelagos; Respiration; Single species; South Pacific; Tropical

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Abstract:

Anthropogenic ocean acidification (OA) is a threat to coral reef fishes, but few studies have investigated responses of high-trophic-level predators, including sharks. We tested the effects of 72-hr exposure to OA-relevant elevated partial pressures of carbon dioxide (pCO2) on oxygen uptake rates, acid–base status, and haematology of newborn tropical blacktip reef sharks (Carcharhinus melanopterus). Acute exposure to end-of-century pCO2 levels resulted in elevated haematocrit (i.e. stress or compensation of oxygen uptake rates) and blood lactate concentrations (i.e. prolonged recovery) in the newborns. Conversely, whole blood and mean corpuscular haemoglobin concentrations, blood pH, estimates of standard and maximum metabolic rates, and aerobic scope remained unaffected. Taken together, newborn blacktip reef sharks appear physiologically robust to end-of-century pCO2 levels, but less so than other, previously investigated, tropical carpet sharks. Our results suggest peak fluctuating pCO2 levels in coral reef lagoons could still physiologically affect newborn reef sharks, but studies assessing the effects of long-term exposure and in combination with other anthropogenic stressors are needed.

Keyword(s):

Animalia; Carcharhinus melanopterus; Chordata; Coast and continental shelf; Laboratory experiment; Mesocosm or benthocosm; Nekton; Other studied parameter or process; Pelagos; Respiration; Single species; South Pacific; Tropical

Supplement to:

Rummer, Jodie L; Bouyoucos, Ian A; Mourier, Johann; Nakamura, Nao; Planes, Serge (2020): Responses of a coral reef shark acutely exposed to ocean acidification conditions. Coral Reefs, 39(5), 1215-1220, https://doi.org/10.1007/s00338-020-01972-0

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2020): seacarb: seawater carbonate chemistry with R. R package version 3.2.14. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-12-25.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Rummer, Jodie L		study
2	Species	Species		Rummer, Jodie L
3	Registration number of species	Reg spec no		Rummer, Jodie L
4	Uniform resource locator/link to reference	URL ref		Rummer, Jodie L		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Rummer, Jodie L
6	Identification	ID		Rummer, Jodie L		Shark
7	Treatment	Treat		Rummer, Jodie L
8	Identification	ID		Rummer, Jodie L		Tank
9	Haematocrit	Ht	%	Rummer, Jodie L
10	Haemoglobin	Hb	mmol/l	Rummer, Jodie L
11	Mean corpuscular haemoglobin concentration	MCHC	mmol/l	Rummer, Jodie L
12	pH	pH		Rummer, Jodie L
13	Lactate	Lactate	mmol/l	Rummer, Jodie L
14	Metabolic rate, maximum	MMR	mg/kg/h	Rummer, Jodie L
15	Metabolic rate, standard	SMR	mg/kg/h	Rummer, Jodie L
16	Absolute aerobic scope	AAS	mg/kg/h	Rummer, Jodie L
17	Mass	Mass	kg	Rummer, Jodie L
18	Temperature, water	Temp	°C	Rummer, Jodie L
19	Mass	Mass	kg	Rummer, Jodie L
20	Mass, standard deviation	Mass std dev	±	Rummer, Jodie L
21	Length	l	cm	Rummer, Jodie L
22	Length, standard deviation	l std dev	±	Rummer, Jodie L
23	Temperature, water	Temp	°C	Rummer, Jodie L
24	Temperature, water, standard deviation	Temp std dev	±	Rummer, Jodie L
25	Salinity	Sal		Rummer, Jodie L
26	Salinity, standard deviation	Sal std dev	±	Rummer, Jodie L
27	pH	pH		Rummer, Jodie L	Potentiometric
28	pH, standard deviation	pH std dev	±	Rummer, Jodie L	Potentiometric
29	Alkalinity, total	AT	µmol/kg	Rummer, Jodie L	Potentiometric titration
30	Alkalinity, total, standard deviation	AT std dev	±	Rummer, Jodie L	Potentiometric titration
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Rummer, Jodie L	Calculated using CO2SYS
32	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Rummer, Jodie L	Calculated using CO2SYS
33	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
35	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

672 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 ID (Shark)	7 Treat	8 ID (Tank)	9 Ht [%]	10 Hb [mmol/l]	11 MCHC [mmol/l]	12 pH	13 Lactate [mmol/l]	14 MMR [mg/kg/h]	15 SMR [mg/kg/h]	16 AAS [mg/kg/h]	17 Mass [kg]	18 Temp [°C]	19 Mass [kg]	20 Mass std dev [±]	21 l [cm]	22 l std dev [±]	23 Temp [°C]	24 Temp std dev [±]	25 Sal	26 Sal std dev [±]	27 pH (Potentiometric)	28 pH std dev [±] (Potentiometric)	29 AT [µmol/kg] (Potentiometric titration)	30 AT std dev [±] (Potentiometric titration)	31 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	32 pCO2 std dev [±] (Calculated using CO2SYS)	33 CSC flag (Calculated using seacarb afte...)	34 pH (total scale, Calculated using...)	35 CO2 [µmol/kg] (Calculated using seacarb afte...)	36 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	37 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	38 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	39 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	40 DIC [µmol/kg] (Calculated using seacarb afte...)	41 Omega Arg (Calculated using seacarb afte...)	42 Omega Cal (Calculated using seacarb afte...)
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	1	CO2	#1	0.290000000	66.0	2.863234828	7.253	8.17	361.3071522	87.481010	273.8261422	1.15	29.39268671	1.2	0.1	59.5	1.3	28.7	0.7	34.5	0.7	7.83	0.03	2294.7	7.6	1048	148.7	26	7.68	27.36	1052.09	1055.31	2005.45	118.49	2151.30	1.92	2.89
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	2	CO2	#1	0.376916868	99.0	3.308980585	7.216	13.82	241.2890600	86.277305	155.0117550	1.35	29.39268671	1.2	0.1	59.5	1.3	28.7	0.7	34.5	0.7	7.83	0.03	2294.7	7.6	1048	148.7	26	7.68	27.36	1052.09	1055.31	2005.45	118.49	2151.30	1.92	2.89
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	3	CO2	#1	0.269906323	58.0	2.701967451	7.400	8.64	400.4869869	85.240890	315.2460969	1.05	29.39268671	1.2	0.1	59.5	1.3	28.7	0.7	34.5	0.7	7.83	0.03	2294.7	7.6	1048	148.7	26	7.68	27.36	1052.09	1055.31	2005.45	118.49	2151.30	1.92	2.89
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	4	CO2	#1	0.333333333	79.5	3.002630550	7.302	8.62	409.5131040	65.892872	343.6202320	1.20	29.39268671	1.2	0.1	59.5	1.3	28.7	0.7	34.5	0.7	7.83	0.03	2294.7	7.6	1048	148.7	26	7.68	27.36	1052.09	1055.31	2005.45	118.49	2151.30	1.92	2.89
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	5	CO2	#2	0.282258065	89.5	3.993515126	7.382	7.99	385.9116710	98.411980	287.4996910	1.05	29.85067543	1.1	0.3	59.7	5.4	28.7	0.7	34.5	0.7	7.83	0.05	2274.6	22.4	1045	78.9	26	7.68	27.12	1042.55	1045.74	1987.45	117.44	2132.00	1.91	2.86
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	6	CO2	#2	0.404185868	89.0	2.773193446	7.438	9.57	375.4110908	75.731780	299.6793108	1.30	29.85067543	1.1	0.3	59.7	5.4	28.7	0.7	34.5	0.7	7.83	0.05	2274.6	22.4	1045	78.9	26	7.68	27.12	1042.55	1045.74	1987.45	117.44	2132.00	1.91	2.86
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	7	CO2	#2	0.419845523	84.5	2.534359165	7.419	7.16	477.0455433	98.682490	378.3630533	1.25	29.85067543	1.1	0.3	59.7	5.4	28.7	0.7	34.5	0.7	7.83	0.05	2274.6	22.4	1045	78.9	26	7.68	27.12	1042.55	1045.74	1987.45	117.44	2132.00	1.91	2.86
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	8	CO2	#2	0.225136159	85.5	4.782314197	7.275	1.21	635.1627346	177.251381	457.9113536	0.65	29.85067543	1.1	0.3	59.7	5.4	28.7	0.7	34.5	0.7	7.83	0.05	2274.6	22.4	1045	78.9	26	7.68	27.12	1042.55	1045.74	1987.45	117.44	2132.00	1.91	2.86
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	9	Control	#6	0.267946162	69.5	3.263908858	7.433	1.14	438.7293248	100.082900	338.6464248	1.10	28.85949123	1.1	0.2	61.5	2.4	28.7	0.8	34.5	0.7	8.04	0.04	2285.3	4.8	599	74.5	26	7.89	15.58	598.98	600.81	1851.19	177.34	2044.10	2.88	4.32
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	10	Control	#6	0.264465225	96.0	4.572673401	7.350	1.01	668.5259076	131.555300	536.9706076	0.85	28.85949123	1.1	0.2	61.5	2.4	28.7	0.8	34.5	0.7	8.04	0.04	2285.3	4.8	599	74.5	26	7.89	15.58	598.98	600.81	1851.19	177.34	2044.10	2.88	4.32
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	11	Control	#6	0.155567858	58.5	4.728446854	7.382	5.82	397.4307855	66.276350	331.1544355	1.30	28.85949123	1.1	0.2	61.5	2.4	28.7	0.8	34.5	0.7	8.04	0.04	2285.3	4.8	599	74.5	26	7.89	15.58	598.98	600.81	1851.19	177.34	2044.10	2.88	4.32
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	12	Control	#6	0.271541950	78.5	3.639380028	7.393	0.99	322.6545309	85.441260	237.2132709	1.10	28.85949123	1.1	0.2	61.5	2.4	28.7	0.8	34.5	0.7	8.04	0.04	2285.3	4.8	599	74.5	26	7.89	15.58	598.98	600.81	1851.19	177.34	2044.10	2.88	4.32
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	13	Control	#5	0.187378100	60.0	4.026847860	7.370	1.33	479.0236605	135.476500	343.5471605	1.00	28.49757244	0.8	0.1	57.5	2.4	28.7	0.8	34.5	0.7	8.03	0.02	2278.3	9.9	608	34.8	26	7.88	15.96	613.55	615.43	1853.32	173.52	2042.80	2.82	4.23
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	14	Control	#5	0.279784946	83.0	3.735335350	7.395	0.45	393.9897783	126.758860	267.2309183	0.70	28.49757244	0.8	0.1	57.5	2.4	28.7	0.8	34.5	0.7	8.03	0.02	2278.3	9.9	608	34.8	26	7.88	15.96	613.55	615.43	1853.32	173.52	2042.80	2.82	4.23
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	15	Control	#5	0.203448276	65.0	4.019230915	7.307	0.51	469.6893713	140.273500	329.4158713	0.75	28.49757244	0.8	0.1	57.5	2.4	28.7	0.8	34.5	0.7	8.03	0.02	2278.3	9.9	608	34.8	26	7.88	15.96	613.55	615.43	1853.32	173.52	2042.80	2.82	4.23
laboratory	Carcharhinus melanopterus (fish)	105795	marinespecies.org	3	16	Control	#5	0.195629159	52.0	3.340417671	7.458	0.75	454.5938673	123.246200	331.3476673	0.90	28.49757244	0.8	0.1	57.5	2.4	28.7	0.8	34.5	0.7	8.03	0.02	2278.3	9.9	608	34.8	26	7.88	15.96	613.55	615.43	1853.32	173.52	2042.80	2.82	4.23