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360 15N2 gas pre-dissolution, which was developed by Mohr et al. (2010), was tested in parallel to the classic method. An underestimation of N2 fixation rates by the classic method has been observed (Großkopf et al., 2012) and therefore the novel 'pre-dissolution method' was applied during the following cruises (M83/1, M90, M91, M93, M97, M104, M107). Single cell N2 fixation rates to differentiate the contribution of different clades of N2 fixers were measured using a NanoSIMS (Martinez-Perez et al., 2016).</gco:CharacterString>
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