Citation:

PANGAEA.926923

Name: Gene expression data of pipefish juveniles Syngnathus typhle (minus delta CT)
Published: 2021-01-20
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Baltic Sea; Climate change; genetic adaptation; Local adaptation; phenotypic plasticity; Salinity; syngnathids; Transgenerational plasticity

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Abstract:

To obtain signatures of local adaptation, transgenerational plasticity and phenotypic plasticity in pipefish originating from different salinity levels we measured targeted gene expression levels in the the offspring generation. This dataset contains -deltaCT values obtained from real time PCR on a fluidigm chip for all juvenile pipefish and targeted genes used in this experiment.

Keyword(s):

Baltic Sea; Climate change; genetic adaptation; Local adaptation; phenotypic plasticity; Salinity; syngnathids; Transgenerational plasticity

Parameter(s):

#	Name	Short Name	Principal Investigator	Method/Device	Comment
1	Species	Species	Goehlich, Henry
2	Sample code/label	Sample label	Goehlich, Henry		Fluidigm Chip run
3	Identification	ID	Goehlich, Henry		Number of juvenile individual
4	Tank number	Tank No	Goehlich, Henry		Tank number in which juveniles were placed after birth
5	Location	Location	Goehlich, Henry		Location at which adults were collected
6	Origin	Origin	Goehlich, Henry		Origin Salinity: Salinity at which adult pipefish were collected: H - High (14 PSU - 17 PSU); L - Low (7 PSU - 11 PSU)
7	Experiment	Exp	Goehlich, Henry		Acclimation Salinity: pipefish adults acclimation in the lab : H - High (15 PSU); L - Low (7 PSU)
8	Experiment	Exp	Goehlich, Henry		Developmental Salinity: juveniles kept after birth and during infection experiment : H - High (15 PSU); L - Low (7 PSU)
9	Treatment	Treat	Goehlich, Henry		Treatment juvenile was exposed to 10 days post hatch c - control; sw - sea water injection; v7 - injection of Vibrio alginolyticus evolved at 7 PSU; v15 - injection of Vibrio alginolyticus evolved at 15 PSU
10	Treatment	Treat	Goehlich, Henry		Injection of juveniles(i.e. sw, v7 or v15) or control Yes - Injection; No - no injection
11	Treatment	Treat	Goehlich, Henry		Origin_salinityxAcclimation_salinity
12	Treatment	Treat	Goehlich, Henry		Acclimation_salinityxDevelopmental_salinity
13	Treatment	Treat	Goehlich, Henry		Origin_salinityxAcclimation_salinityxDevelopmental_salinity
14	Treatment	Treat	Goehlich, Henry		Interaction
15	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	lymphag75: Lymphocyte antigen 75
16	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	HIVEP2: Human immunodeficiency virus type l enhancer 2
17	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	HIVEP3: Human immunodeficiency virus type l enhancer 3
18	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	CD45: CD45 (Leukocyte common antigen)
19	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	IgM: Immunoglobulin light chain
20	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	cf: Coagulation factor II
21	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	hsp60: Heat shock protein 60 kDa
22	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	il10: Interleukin 10
23	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	kin: Kinesin
24	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	aif: Allograft inflammatory factor
25	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	tspo: Translocator protein
26	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	tranfe: Transferrin
27	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	il8: Interleukin 8
28	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	tyroprot: Tyroproteinkinase
29	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	No66: Lysine-specific histone demethylase NO66
30	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	TPR: Lysine-specific demethylase 6A
31	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	DnMt3B: DNA methyltransferase 3b
32	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	N6admet: N(6)- adenine-specific DNAmethyltransferase 2
33	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	ASH: Histone methyltransferase
34	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	apoa1: Apolipoprotein A1
35	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	aqp3: Aquaporin 3
36	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	atp1a1: ATPase alpha 1
37	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	bcell rap31: B-cell receptor-associated protein
38	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	C1: Recognition subcomponent C1
39	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	C3: Complement component 3
40	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	C9: Complement component 9
41	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	CFTR: Cystic fibrosis transmembrane conductance regulator
42	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	cldn1: Claudin 1
43	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	ddpgly: Ddp-glycosyltransferase
44	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	g6DPH: Glucose 6 phosphate dehydrogenase (G6PD)
45	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	HDAC1: Histone deacetylase 1-like
46	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	HDAC3: Histone deacetylase 3-like
47	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	HSP70: Heat shock protein 70 kDa
48	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	JmicPhD: Lysine-specific demethylase 5B
49	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	KCNH8: Voltage gated potassium channel subfamily h member 8
50	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	lectp2: Lectin protein type II
51	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	mapk8ip3: Mitogen-activated protein kinase 8 interacting protein 3
52	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	MYST: Histone acetyltransferase
53	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	NKCC2: Na+/K+ /2Cl cotransporter (Slc12A1)
54	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	NR3C1_A: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptors) _
55	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	NR3C1_B: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptors) _
56	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	PRL: Prolactin
57	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	PRLR: Prolactin receptor
58	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	ptgis: Prostaglandin I2 Synthase
59	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	ripop: Ribosomal protein
60	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	TNF: Tumor necrosis factor
61	Minus Δ threshold cycle, quantitative polymerase chain reaction	Minus Δ Ct qPCR	Goehlich, Henry	High Throughput PCR, Fluidigm BioMark HD	ubi: Ubiquitin

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

34099 data points

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