Citation:

PANGAEA.925549

Name: Seawater carbonate chemistry and motility of flagellated microalgae
Published: 2020-12-09
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Behaviour; Bottles or small containers/Aquaria (<20 L); Chlamydomonas reinhardtii; Chlorophyta; Dunaliella salina; Laboratory experiment; Laboratory strains; Microglena sp.; Not applicable; Pelagos; Phytoplankton; Plantae; Respiration; Single species

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Abstract:

Motility plays a critical role in algal survival and reproduction, with implications for aquatic ecosystem stability. However, the effect of elevated CO2 on marine, brackish and freshwater algal motility is unclear. Here we show, using laboratory microscale and field mesoscale experiments, that three typical phytoplankton species had decreased motility with increased CO2. Polar marine Microglena sp., euryhaline Dunaliella salina and freshwater Chlamydomonas reinhardtii were grown under different CO2 concentrations for 5 years. Long-term acclimated Microglena sp. showed substantially decreased photo-responses in all treatments, with a photophobic reaction affecting intracellular calcium concentration. Genes regulating flagellar movement were significantly downregulated (P < 0.05), alongside a significant increase in gene expression for flagellar shedding (P < 0.05). D. salina and C. reinhardtii showed similar results, suggesting that motility changes are common across flagellated species. As the flagella structure and bending mechanism are conserved from unicellular organisms to vertebrates, these results suggest that increasing surface water CO2 concentrations may affect flagellated cells from algae to fish.

Keyword(s):

Behaviour; Bottles or small containers/Aquaria (<20 L); Chlamydomonas reinhardtii; Chlorophyta; Dunaliella salina; Laboratory experiment; Laboratory strains; Microglena sp.; Not applicable; Pelagos; Phytoplankton; Plantae; Respiration; Single species

Supplement to:

Wang, Yitao; Fan, Xiao; Gao, Guang; Beardall, John; Inaba, Kazuo; Hall-Spencer, Jason M; Xu, Dong; Zhang, Xiaowen; Han, Wentao; McMinn, Andrew; Ye, Naihao (2020): Decreased motility of flagellated microalgae long-term acclimated to CO2-induced acidified waters. Nature Climate Change, 10(6), 561-567, https://doi.org/10.1038/s41558-020-0776-2

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2020): seacarb: seawater carbonate chemistry with R. R package version 3.2.14. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-12-08.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Ye, Naihao		study
2	Species	Species		Ye, Naihao
3	Registration number of species	Reg spec no		Ye, Naihao
4	Uniform resource locator/link to reference	URL ref		Ye, Naihao		WoRMS Aphia ID
5	Figure	Fig		Ye, Naihao
6	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Ye, Naihao
7	Move velocity	Move V	µm/s	Ye, Naihao		Positive phototaxis induced by white light in the vertical direction
8	Move velocity	Move V	µm/s	Ye, Naihao		Negative phototaxis induced by white light in the vertical direction
9	Move velocity	Move V	µm/s	Ye, Naihao		Positive phototaxis induced by white light in the horizontal direction
10	Move velocity	Move V	µm/s	Ye, Naihao		Negative phototaxis induced by white light in the horizontal direction
11	Move velocity	Move V	µm/s	Ye, Naihao		Positive phototaxis in the vertical direction
12	Move velocity	Move V	µm/s	Ye, Naihao		Negative phototaxis in the vertical direction
13	Move velocity	Move V	µm/s	Ye, Naihao		Positive phototaxis in the horizontal direction
14	Move velocity	Move V	µm/s	Ye, Naihao		Negative phototaxis in the horizontal direction
15	Percentage	Perc	%	Ye, Naihao		Deflagellation
16	Percentage	Perc	%	Ye, Naihao		motile cells after 1 hour
17	Percentage	Perc	%	Ye, Naihao		motile cells after 3 hour
18	Gene name	Gene		Ye, Naihao
19	Gene expression	Gene expression		Ye, Naihao
20	Calcium, flux	Ca flux	pmol/cm/s	Ye, Naihao		efflux under positive phototaxis
21	Calcium, flux	Ca flux	pmol/cm/s	Ye, Naihao		influx under negative phototaxis
22	Irradiance	E	µmol/m²/s	Ye, Naihao
23	Oxygen evolution, per chlorophyll a	O2 ev/Chl	mmol/mol/s	Ye, Naihao
24	Daily vertical migration	DVM	m	Ye, Naihao
25	Time in days	Time	days	Ye, Naihao		DVM cross time, positive phototaxis using average velocity
26	Time in days	Time	days	Ye, Naihao		DVM cross time, positive phototaxis using instantaneous velocity
27	Time in days	Time	days	Ye, Naihao		DVM cross time, positive phototaxis using instantaneous velocity
28	Time in days	Time	days	Ye, Naihao		DVM cross time, negative phototaxis using average velocity
29	Temperature, water	Temp	°C	Ye, Naihao
30	Salinity	Sal		Ye, Naihao
31	pH	pH		Ye, Naihao	Potentiometric	total scale
32	pH, standard error	pH std e	±	Ye, Naihao	Potentiometric	total scale
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Ye, Naihao	Calculated using CO2SYS
34	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Ye, Naihao	Calculated using CO2SYS
35	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Ye, Naihao	Calculated using CO2SYS
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Ye, Naihao	Calculated using CO2SYS
37	Alkalinity, total	AT	µmol/kg	Ye, Naihao	Potentiometric titration
38	Alkalinity, total, standard error	AT std e	±	Ye, Naihao	Potentiometric titration
39	Carbon, inorganic, dissolved	DIC	µmol/kg	Ye, Naihao	Calculated using CO2SYS
40	Carbon, inorganic, dissolved, standard error	DIC std e	±	Ye, Naihao	Calculated using CO2SYS
41	Carbon dioxide	CO2	µmol/kg	Ye, Naihao	Calculated using CO2SYS
42	Carbon dioxide, standard error	CO2 std e	±	Ye, Naihao	Calculated using CO2SYS
43	Carbonate ion	[CO3]2-	µmol/kg	Ye, Naihao	Calculated using CO2SYS
44	Carbonate ion, standard error	[CO3]2- std e	±	Ye, Naihao	Calculated using CO2SYS
45	Bicarbonate ion	[HCO3]-	µmol/kg	Ye, Naihao	Calculated using CO2SYS
46	Bicarbonate ion, standard error	[HCO3]- std e	±	Ye, Naihao	Calculated using CO2SYS
47	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

124767 data points

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