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Howald, Sarah; Cominassi, Louise; LeBayon, Nicolas; Claireaux, Guy; Mark, Felix Christopher (2020): Respiration rates of permeabilized heart fibres and biometrical data of European seabass Dicentrarchus labrax [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.925190, In: Howald, S et al. (2020): Experimental conditions, respiration rates of permeabilized heart fibres and biometrical data of European seabass Dicentrarchus labrax [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.925193

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Supplement to:
Howald, Sarah; Cominassi, Louise; LeBayon, Nicolas; Claireaux, Guy; Mark, Felix Christopher (2019): Future ocean warming may prove beneficial for the northern population of European seabass, but ocean acidification will not. Journal of Experimental Biology, 222(21), jeb213017, https://doi.org/10.1242/jeb.213017
Further details:
Pesta, Dominik; Gnaiger, Erich (2012): High-resolution respirometry: OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. In: Palmeira, Carlos M.; Moreno, António J. (eds.), Mitochondrial Bioenergetics, Methods in Molecular Biology, 810, Humana Press, Totowa, NJ, 25-58, https://doi.org/10.1007/978-1-61779-382-0_3
Saks, V A; Veksler, Vladimir I; Kuznetsov, Andrei V; Kay, Laurence; Sikk, Peeter; Tiivel, Toomas; Tranqui, Leone; Olivares, Jose; Wiedemann, Falk; Kunz, Wolfram S (1998): Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vitro. Molecular and Cellular Biochemistry, 184(1/2), 81-100, https://doi.org/10.1023/A:1006834912257
Coverage:
Date/Time Start: 2016-07-19T00:00:00 * Date/Time End: 2016-09-23T00:00:00
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Sample IDSample IDHowald, Sarah
2TreatmentTreatHowald, Sarah
3Treatment: temperature descriptionT:Temp descrHowald, Sarahof life conditioning
4DATE/TIMEDate/TimeHowald, SarahGeocode
5InstrumentInstrumentHowald, Sarahoxygraph number
6IdentificationIDHowald, Sarahchamber
7Run IDRun IDHowald, Sarah
8Temperature, waterTemp°CHowald, Sarahassay
9Tank numberTank NoHowald, Sarahof origin
10Specimen identificationSpec IDHowald, Sarah
11Body lengthBLmmHowald, SarahMeasured using callipers
12Dicentrarchus labrax, massD. labrax mgHowald, SarahFish, wet weightedcarcasse
13Heart, massHeart mgHowald, SarahWeightedventricle only
14Tissue, massTis massgHowald, SarahWeightedventricle tissue used for assay
15Liver, massLiver mgHowald, SarahWeighted
16Fulton's condition factorKHowald, SarahCalculated
17Hepatosomatic indexHSIHowald, SarahCalculated
18Mitochondrial respiration rate, complex IPCIpmol/mg/sHowald, SarahRespirometer, Oroboros Oxygraph-2K
19Mitochondrial respiration rate, electron transportPpmol/mg/sHowald, SarahRespirometer, Oroboros Oxygraph-2K
20Mitochondrial respiration rate, complex IIPCIIpmol/mg/sHowald, SarahRespirometer, Oroboros Oxygraph-2K
21Mitochondrial leak respiration rateLOmypmol/mg/sHowald, SarahRespirometer, Oroboros Oxygraph-2K
22Mitochondrial respiration rate, complex IVCIV resppmol/mg/sHowald, SarahRespirometer, Oroboros Oxygraph-2K
23Respiratory control ratioRCROmyHowald, SarahCalculated
24Complex I respiration rate of total mitochondrial respiration ratePCI%Howald, SarahCalculated
25Complex II respiration rate of total mitochondrial respiration ratePCII%Howald, SarahCalculated
26Proton leak of total mitochondrial respiration rateLOmy%Howald, SarahCalculated
27Mitochondrial complex IV capacityCIV cap%Howald, SarahCalculated
Size:
4138 data points

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