Relitti, Federica; Ogrinc, Nives; Giani, Michele; Cerino, Federica; Smodlaka Tanković, Mirta; Baričević, Ana; Urbini, Lidia; Krajnc, Bor; Celussi, Mauro; Kralj, Martina; De Vittor, Cinzia (2020): Thalassiosira rotula culture experiments for the assessment of phytoplankton stable carbon isotopic composition as a tool to monitor anthropogenic CO2 submarine leakages [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.925185
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Published: 2020-11-26 • DOI registered: 2020-12-18
Abstract:
Three culture experiment on the diatom Thalassiosira rotula were conducted within two 2L-photobioreactors under controlled conditions of light and temperature (20 ± 0.02 °C). During each experiment, diatoms were simultaneously grown in the two bioreactors filled respectively with different media. In one of the two bioreactor microalgae were grown in a natural seawater (NAT) medium, whereas in the second one an artificial seawater medium (ASW) was used. ASW medium was artificially prepared, to reach a modified isotopic composition of DIC using an industrial compressed CO2, and at each experiment a different initial DIC concentration was tested to verify if carbon availability would affect phytoplankton algal metabolism. For NAT medium, a sterile-filtered Adriatic Sea water was used and a natural DIC concentration used. During the experiments, the evolution of T. rotula abundance was investigated, together with the variation of the isotopic composition of dissolved inorganic carbon, phytoplankton organic carbon (as particulate organic carbon) and prokaryotes organic carbon (as the fraction < 10 µm of particulate organic carbon). Carbonate system parameters (in situ pHNBS, total alkalinity, DIC and dissolved CO2 concentration), nutrient (dissolved inorganic nitrogen and phosphate) concentration and prokaryotes abundance were also analysed during diatom cultivation.
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Supplement to:
Relitti, Federica; Ogrinc, Nives; Giani, Michele; Cerino, Federica; Smodlaka Tanković, Mirta; Baričević, Ana; Urbini, Lidia; Krajnc, Bor; Celussi, Mauro; Kralj, Martina; De Vittor, Cinzia (accepted): Stable carbon isotopes of phytoplankton as a tool to monitor anthropogenic CO2 submarine leakages. Water
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Experiment | Exp | De Vittor, Cinzia | 2L-photobioreactors | First (EXP-1), second (EXP-2), third (EXP-3) culture experiment | |
| 2 | Medium | Medium | Giani, Michele | Artificial (ASW) or natural (NAT) seawater medium | ||
| 3 | Code | Code | Relitti, Federica | |||
| 4 | Time in hours | Time h | h | Relitti, Federica | Sampling time (hours) after T. rotula inoculation | |
| 5 | Thalassiosira rotula | T. rotula | #/l | Cerino, Federica | Light microscope | |
| 6 | Thalassiosira rotula, standard deviation | T. rotula std dev | ± | Cerino, Federica | Calculated | |
| 7 | δ13C, dissolved inorganic carbon | δ13C DIC | ‰ PDB | Ogrinc, Nives | Continuous-flow IRMS Europa 20-20 with ANCA TG trace gas separation module | Analysis performed by gas evolution method with phosphoric acid |
| 8 | Phytoplankton, δ13C, particulate organic carbon | Phytopl δ13C POC | ‰ PDB | Ogrinc, Nives | IRMS Europa 20-20 with an ANCA-SL preparation module for solid and liquid samples | Analysis performed as bulk particulate organic carbon on pre-combusted (450 °C for 4 h) Whatman GF/F filters (nominal porosity 0.7 µm, ø 25 mm) |
| 9 | Carbon, inorganic, dissolved | DIC | µmol/l | Urbini, Lidia | Calculated using CO2SYS | Calculated using CO2Sys program (Pierrot et al. 2006). Carbonic acid dissociation constants from Mehrbach et al. (1973) refitted by Dickson and Millero (1987), constant for HSO4 from Dickson (1990), borate dissociation constant from Uppström (1974). Data were converted in µmol/l using medium density |
| 10 | Alkalinity, total | AT | µmol/kg | Urbini, Lidia | Potentiometric open-cell titration | |
| 11 | pH, NBS scale | pH NBS | Urbini, Lidia | Electrochemical probe | pH(NBS) in situ, Hamilton EasyFerm BIO Arc 225 probes, calibrated on the National Bureau of Standards (NBS) scale with certified buffer solutions of pH 7 ± 0.02 and 9 ± 0.03 (at 25 °C, Merck Certipur®) | |
| 12 | Nitrogen, inorganic, dissolved | DIN | µmol/l | Kralj, Martina | Autoanalyzer-Colorimeter | |
| 13 | Phosphate | [PO4]3- | µmol/l | Kralj, Martina | Autoanalyzer-Colorimeter | |
| 14 | Prokaryotes | Prokaryotes | #/l | Celussi, Mauro | Flow-cytometer | |
| 15 | Prokaryotes, δ13C, particulate organic carbon | Prok δ13C POC | ‰ PDB | Ogrinc, Nives | IRMS Europa 20-20 with an ANCA-SL preparation module for solid and liquid samples | Analysis performed as bulk particulate organic carbon on pre-combusted (450 °C for 4 h) Whatman GF/F filters (nominal porosity 0.7 µm, ø 25 mm) after a pre-filtration on 10 µm nylon mesh to remove phytoplankton cells |
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Size:
433 data points