Citation:

PANGAEA.925182

Name: Seawater carbonate chemistry and larval settlement of Sea urchin larvae
Published: 2020-11-26
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Echinodermata; Evechinus chloroticus; Laboratory experiment; Pelagos; Reproduction; Single species; South Pacific; Temperate; Zooplankton

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Abstract:

Extensive research has shown that the early life stages of marine organisms are sensitive to ocean acidification (OA). Less is known, however, on whether larval settlement and metamorphosis may be affected, or by which mechanisms. These are key processes in the life cycle of most marine benthic organisms, since they mark the transition between the free swimming larval stage to the benthic life. We investigated whether OA could affect the larval settlement success of the sea urchin Evechinus chloroticus, a key coastal species with ecological, economic and cultural importance in New Zealand. We performed four settlement experiments to test whether reduced seawater pH (ranging from 8.1 to 7.0, at an interval of ∼0.2 pH units) alters larval settlement and metamorphosis success. Our results show that settlement success was not significantly reduced when the larvae were exposed to a range of reduced seawater pH treatments (8.1–7.0) at time of settlement (direct effects). Similarly, when presented with crustose coralline algae (CCA) pre-conditioned in different seawater pH of either pH 8.1 or 7.7 for 28 days, larval settlement success remained unaltered (indirect effects). We conclude that competent larvae in this species are resilient to OA at time of settlement. Further research on a range of taxa that vary in settlement selectivity and behaviour is needed in order to fully understand the effects of OA on the life cycle of marine invertebrates and the consequences it might have for future coastal marine ecosystems.

Keyword(s):

Animalia; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Echinodermata; Evechinus chloroticus; Laboratory experiment; Pelagos; Reproduction; Single species; South Pacific; Temperate; Zooplankton

Supplement to:

Espinel-Velasco, Nadjejda; Agüera, Antonio; Lamare, Miles (2020): Sea urchin larvae show resilience to ocean acidification at the time of settlement and metamorphosis. Marine Environmental Research, 159, 104977, https://doi.org/10.1016/j.marenvres.2020.104977

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2020): seacarb: seawater carbonate chemistry with R. R package version 3.2.14. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-11-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Espinel-Velasco, Nadjejda		study
2	Species	Species		Espinel-Velasco, Nadjejda
3	Registration number of species	Reg spec no		Espinel-Velasco, Nadjejda
4	Uniform resource locator/link to reference	URL ref		Espinel-Velasco, Nadjejda		WoRMS Aphia ID
5	Experiment	Exp		Espinel-Velasco, Nadjejda
6	Treatment	Treat		Espinel-Velasco, Nadjejda
7	Substrate type	Substrate		Espinel-Velasco, Nadjejda		Settlement
8	Time in hours	Time	h	Espinel-Velasco, Nadjejda		after
9	Settlement	Settlem	%	Espinel-Velasco, Nadjejda
10	Settlement, standard error	Settlem std e	±	Espinel-Velasco, Nadjejda
11	pH	pH		Espinel-Velasco, Nadjejda		NBS scale
12	pH, standard error	pH std e	±	Espinel-Velasco, Nadjejda		NBS scale
13	pH	pH		Espinel-Velasco, Nadjejda		total scale
14	pH, standard error	pH std e	±	Espinel-Velasco, Nadjejda		total scale
15	Condition	Cond		Espinel-Velasco, Nadjejda		CCA
16	Salinity	Sal		Espinel-Velasco, Nadjejda
17	Temperature, water	Temp	°C	Espinel-Velasco, Nadjejda
18	Alkalinity, total	AT	µmol/kg	Espinel-Velasco, Nadjejda
19	Carbon, inorganic, dissolved	DIC	µmol/kg	Espinel-Velasco, Nadjejda
20	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Espinel-Velasco, Nadjejda	Calculated using SWCO2 (Hunter, 2007)
21	Carbonate ion	[CO3]2-	µmol/kg	Espinel-Velasco, Nadjejda	Calculated using SWCO2 (Hunter, 2007)
22	Calcite saturation state	Omega Cal		Espinel-Velasco, Nadjejda	Calculated using SWCO2 (Hunter, 2007)
23	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
25	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1999 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp	6 Treat	7 Substrate (Settlement)	8 Time [h] (after)	9 Settlem [%]	10 Settlem std e [±]	11 pH (NBS scale)	12 pH std e [±] (NBS scale)	13 pH (total scale)	14 pH std e [±] (total scale)	15 Cond (CCA)	16 Sal	17 Temp [°C]	18 AT [µmol/kg]	19 DIC [µmol/kg]	20 pCO2water_SST_wet [µatm] (Calculated using SWCO2 (Hunte...)	21 [CO3]2- [µmol/kg] (Calculated using SWCO2 (Hunte...)	22 Omega Cal (Calculated using SWCO2 (Hunte...)	23 CSC flag (Calculated using seacarb afte...)	24 pH (total scale, Calculated using...)	25 CO2 [µmol/kg] (Calculated using seacarb afte...)	26 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	27 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	28 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	29 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	30 Omega Arg (Calculated using seacarb afte...)	31 Omega Cal (Calculated using seacarb afte...)
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 8.1	CCA	8	13.87	5.74	8.06	0.020				34.5	10.1	2263.7	2100.8	434.5	121.4	2.90	15	8.01	18.98	432.80	434.47	1960.58	121.24	1.84	2.90
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 8.1	Bare rock	8	0.00		8.09	0.004				34.5	10.1	2263.7	2100.8	434.5	121.4	2.90	15	8.01	18.98	432.80	434.47	1960.58	121.24	1.84	2.90
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.9	CCA	8	14.76	6.44	7.89	0.001				34.5	10.1	2266.1	2251.6	1316.0	48.0	1.15	15	7.57	57.51	1311.15	1316.21	2146.14	47.95	0.73	1.15
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.9	Bare rock	8	0.00		7.90	0.002				34.5	10.1	2266.1	2251.6	1316.0	48.0	1.15	15	7.57	57.51	1311.15	1316.21	2146.14	47.95	0.73	1.15
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.7	CCA	8	19.45	5.47	7.63	0.011				34.5	10.1	2269.4	2251.7	1282.0	49.2	1.18	15	7.58	56.10	1279.03	1283.98	2146.43	49.17	0.75	1.18
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.7	Bare rock	8	0.00		7.65	0.007				34.5	10.1	2269.4	2251.7	1282.0	49.2	1.18	15	7.58	56.10	1279.03	1283.98	2146.43	49.17	0.75	1.18
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 8.1	CCA	16	28.84	3.60	8.08	0.010				34.5	10.1	2263.7	2100.8	434.5	121.4	2.90	15	8.01	18.98	432.80	434.47	1960.58	121.24	1.84	2.90
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 8.1	Bare rock	16	0.00		8.11	0.003				34.5	10.1	2263.7	2100.8	434.5	121.4	2.90	15	8.01	18.98	432.80	434.47	1960.58	121.24	1.84	2.90
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.9	CCA	16	21.57	9.73	7.94	0.026				34.5	10.1	2266.1	2251.6	1316.0	48.0	1.15	15	7.57	57.51	1311.15	1316.21	2146.14	47.95	0.73	1.15
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.9	Bare rock	16	0.00		7.94	0.014				34.5	10.1	2266.1	2251.6	1316.0	48.0	1.15	15	7.57	57.51	1311.15	1316.21	2146.14	47.95	0.73	1.15
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.7	CCA	16	31.10	6.57	7.65	0.005				34.5	10.1	2269.4	2251.7	1282.0	49.2	1.18	15	7.58	56.10	1279.03	1283.98	2146.43	49.17	0.75	1.18
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.7	Bare rock	16	1.15	1.15	7.65	0.006				34.5	10.1	2269.4	2251.7	1282.0	49.2	1.18	15	7.58	56.10	1279.03	1283.98	2146.43	49.17	0.75	1.18
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 8.1	CCA	24	41.20	3.05	8.08	0.012				34.5	10.1	2263.7	2100.8	434.5	121.4	2.90	15	8.01	18.98	432.80	434.47	1960.58	121.24	1.84	2.90
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 8.1	Bare rock	24	0.00		8.11	0.003				34.5	10.1	2263.7	2100.8	434.5	121.4	2.90	15	8.01	18.98	432.80	434.47	1960.58	121.24	1.84	2.90
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.9	CCA	24	27.38	12.30	7.95	0.013				34.5	10.1	2266.1	2251.6	1316.0	48.0	1.15	15	7.57	57.51	1311.15	1316.21	2146.14	47.95	0.73	1.15
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.9	Bare rock	24	0.00		7.94	0.008				34.5	10.1	2266.1	2251.6	1316.0	48.0	1.15	15	7.57	57.51	1311.15	1316.21	2146.14	47.95	0.73	1.15
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.7	CCA	24	33.01	4.98	7.64	0.002				34.5	10.1	2269.4	2251.7	1282.0	49.2	1.18	15	7.58	56.10	1279.03	1283.98	2146.43	49.17	0.75	1.18
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.7	Bare rock	24	2.16	1.09	7.66	0.004				34.5	10.1	2269.4	2251.7	1282.0	49.2	1.18	15	7.58	56.10	1279.03	1283.98	2146.43	49.17	0.75	1.18
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 8.1	CCA	32	48.89	7.55	8.05	0.017				34.5	10.1	2263.7	2100.8	434.5	121.4	2.90	15	8.01	18.98	432.80	434.47	1960.58	121.24	1.84	2.90
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 8.1	Bare rock	32	0.79	0.79	8.09	0.002				34.5	10.1	2263.7	2100.8	434.5	121.4	2.90	15	8.01	18.98	432.80	434.47	1960.58	121.24	1.84	2.90
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.9	CCA	32	35.21	16.68	7.94	0.036				34.5	10.1	2266.1	2251.6	1316.0	48.0	1.15	15	7.57	57.51	1311.15	1316.21	2146.14	47.95	0.73	1.15
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.9	Bare rock	32	0.00		7.91	0.018				34.5	10.1	2266.1	2251.6	1316.0	48.0	1.15	15	7.57	57.51	1311.15	1316.21	2146.14	47.95	0.73	1.15
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.7	CCA	32	45.59	13.85	7.65	0.006				34.5	10.1	2269.4	2251.7	1282.0	49.2	1.18	15	7.58	56.10	1279.03	1283.98	2146.43	49.17	0.75	1.18
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	1	pH 7.7	Bare rock	32	2.16	1.09	7.66	0.004				34.5	10.1	2269.4	2251.7	1282.0	49.2	1.18	15	7.58	56.10	1279.03	1283.98	2146.43	49.17	0.75	1.18
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 8.1		6	49.86	5.04	8.16	0.010	8.05	0.010		34.3	14.0	2269.9	2095.3	484.7	130.5	3.12	15	7.98	18.38	474.16	475.90	1946.84	130.08	1.99	3.11
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 7.9		6	60.93	5.95	8.03	0.020	7.91	0.020		34.3	14.0	2262.9	2146.4	707.1	96.8	2.32	15	7.83	26.78	690.57	693.11	2023.17	96.45	1.48	2.31
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 7.7		6	55.87	2.25	7.71	0.003	7.60	0.003		34.3	14.0	2268.9	2249.8	1491.3	51.3	1.23	15	7.53	56.69	1462.08	1467.46	2142.04	51.07	0.78	1.22
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 8.1		12	64.17	3.63	8.13	0.020	8.02	0.020		34.3	14.0	2269.9	2095.3	484.7	130.5	3.12	15	7.98	18.38	474.16	475.90	1946.84	130.08	1.99	3.11
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 7.9		12	73.99	3.93	7.97	0.010	7.86	0.010		34.3	14.0	2262.9	2146.4	707.1	96.8	2.32	15	7.83	26.78	690.57	693.11	2023.17	96.45	1.48	2.31
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 7.7		12	64.41	1.14	7.74	0.020	7.63	0.020		34.3	14.0	2268.9	2249.8	1491.3	51.3	1.23	15	7.53	56.69	1462.08	1467.46	2142.04	51.07	0.78	1.22
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 8.1		18	74.10	1.66	8.09	0.040	7.98	0.040		34.3	14.0	2269.9	2095.3	484.7	130.5	3.12	15	7.98	18.38	474.16	475.90	1946.84	130.08	1.99	3.11
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 7.9		18	76.53	4.22	7.95	0.010	7.84	0.010		34.3	14.0	2262.9	2146.4	707.1	96.8	2.32	15	7.83	26.78	690.57	693.11	2023.17	96.45	1.48	2.31
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 7.7		18	77.63	3.49	7.73	0.030	7.62	0.030		34.3	14.0	2268.9	2249.8	1491.3	51.3	1.23	15	7.53	56.69	1462.08	1467.46	2142.04	51.07	0.78	1.22
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 8.1		30	78.70	0.66	8.04	0.030	7.93	0.030		34.3	14.0	2269.9	2095.3	484.7	130.5	3.12	15	7.98	18.38	474.16	475.90	1946.84	130.08	1.99	3.11
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 7.9		30	82.45	5.66	7.93	0.010	7.82	0.010		34.3	14.0	2262.9	2146.4	707.1	96.8	2.32	15	7.83	26.78	690.57	693.11	2023.17	96.45	1.48	2.31
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 7.7		30	82.80	3.15	7.74	0.020	7.63	0.020		34.3	14.0	2268.9	2249.8	1491.3	51.3	1.23	15	7.53	56.69	1462.08	1467.46	2142.04	51.07	0.78	1.22
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 8.1		48	81.57	4.31	7.96	0.030	7.85	0.030		34.3	14.0	2269.9	2095.3	484.7	130.5	3.12	15	7.98	18.38	474.16	475.90	1946.84	130.08	1.99	3.11
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 7.9		48	83.15	5.33	7.92	0.010	7.81	0.010		34.3	14.0	2262.9	2146.4	707.1	96.8	2.32	15	7.83	26.78	690.57	693.11	2023.17	96.45	1.48	2.31
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	2	pH 7.7		48	83.64	2.36	7.62	0.010	7.73	0.010		34.3	14.0	2268.9	2249.8	1491.3	51.3	1.23	15	7.53	56.69	1462.08	1467.46	2142.04	51.07	0.78	1.22
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 8.1		3	8.66	2.38			8.02	0.001		34.6	10.9	2268.1	2087.2	404.6	132.5	3.16	15	8.04	17.20	402.86	404.39	1937.68	132.32	2.01	3.16
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.9		3	10.55	1.73			7.87	0.004		34.6	10.9	2271.9	2164.4	659.2	90.7	2.17	15	7.85	28.03	656.52	659.03	2045.85	90.51	1.38	2.16
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.7		3	6.79	4.28			7.60	0.005		34.6	10.9	2256.3	2246.5	1403.2	46.4	1.11	15	7.54	59.86	1401.83	1407.20	2140.25	46.39	0.71	1.11
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.4		3	7.24	1.00			7.28	0.011		34.6	10.9	2270.0	2356.2	2872.3	24.4	0.58	15	7.24	122.40	2866.34	2877.33	2209.62	24.18	0.37	0.58
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.2		3	2.95	1.38			7.22	0.008		34.6	10.9	2268.6	2389.0	3527.1	19.9	0.48	15	7.15	150.14	3515.96	3529.45	2218.98	19.88	0.30	0.47
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.0		3	7.60	3.16			6.90	0.006		34.6	10.9	2281.0	2544.4	6567.3	11.0	0.26	15	6.89	279.72	6550.43	6575.63	2253.68	11.01	0.17	0.26
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 8.1		6	27.73	3.04			7.96	0.005		34.6	10.9	2268.1	2087.2	404.6	132.5	3.16	15	8.04	17.20	402.86	404.39	1937.68	132.32	2.01	3.16
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.9		6	35.88	4.79			7.82	0.014		34.6	10.9	2271.9	2164.4	659.2	90.7	2.17	15	7.85	28.03	656.52	659.03	2045.85	90.51	1.38	2.16
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.7		6	22.00	4.58			7.59	0.011		34.6	10.9	2256.3	2246.5	1403.2	46.4	1.11	15	7.54	59.86	1401.83	1407.20	2140.25	46.39	0.71	1.11
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.4		6	16.01	3.53			7.32	0.011		34.6	10.9	2270.0	2356.2	2872.3	24.4	0.58	15	7.24	122.40	2866.34	2877.33	2209.62	24.18	0.37	0.58
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.2		6	24.32	2.16			7.25	0.010		34.6	10.9	2268.6	2389.0	3527.1	19.9	0.48	15	7.15	150.14	3515.96	3529.45	2218.98	19.88	0.30	0.47
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.0		6	17.02	4.56			6.95	0.017		34.6	10.9	2281.0	2544.4	6567.3	11.0	0.26	15	6.89	279.72	6550.43	6575.63	2253.68	11.01	0.17	0.26
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 8.1		12	43.24	0.28			7.99	0.013		34.6	10.9	2268.1	2087.2	404.6	132.5	3.16	15	8.04	17.20	402.86	404.39	1937.68	132.32	2.01	3.16
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.9		12	52.68	4.48			7.90	0.009		34.6	10.9	2271.9	2164.4	659.2	90.7	2.17	15	7.85	28.03	656.52	659.03	2045.85	90.51	1.38	2.16
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.7		12	49.60	2.39			7.66	0.021		34.6	10.9	2256.3	2246.5	1403.2	46.4	1.11	15	7.54	59.86	1401.83	1407.20	2140.25	46.39	0.71	1.11
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.4		12	45.85	6.53			7.37	0.001		34.6	10.9	2270.0	2356.2	2872.3	24.4	0.58	15	7.24	122.40	2866.34	2877.33	2209.62	24.18	0.37	0.58
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.2		12	52.26	7.48			7.26	0.005		34.6	10.9	2268.6	2389.0	3527.1	19.9	0.48	15	7.15	150.14	3515.96	3529.45	2218.98	19.88	0.30	0.47
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.0		12	38.39	5.43			7.02	0.009		34.6	10.9	2281.0	2544.4	6567.3	11.0	0.26	15	6.89	279.72	6550.43	6575.63	2253.68	11.01	0.17	0.26
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 8.1		24	57.37	4.96			8.01	0.023		34.6	10.9	2268.1	2087.2	404.6	132.5	3.16	15	8.04	17.20	402.86	404.39	1937.68	132.32	2.01	3.16
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.9		24	60.73	3.05			7.86	0.005		34.6	10.9	2271.9	2164.4	659.2	90.7	2.17	15	7.85	28.03	656.52	659.03	2045.85	90.51	1.38	2.16
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.7		24	57.09	4.75			7.62	0.010		34.6	10.9	2256.3	2246.5	1403.2	46.4	1.11	15	7.54	59.86	1401.83	1407.20	2140.25	46.39	0.71	1.11
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.4		24	51.56	3.64			7.35	0.007		34.6	10.9	2270.0	2356.2	2872.3	24.4	0.58	15	7.24	122.40	2866.34	2877.33	2209.62	24.18	0.37	0.58
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.2		24	62.03	8.90			7.27	0.033		34.6	10.9	2268.6	2389.0	3527.1	19.9	0.48	15	7.15	150.14	3515.96	3529.45	2218.98	19.88	0.30	0.47
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	3	pH 7.0		24	48.75	9.18			7.01	0.002		34.6	10.9	2281.0	2544.4	6567.3	11.0	0.26	15	6.89	279.72	6550.43	6575.63	2253.68	11.01	0.17	0.26
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 8.1		8	66.68	1.95			7.96	0.015	High	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 8.1		8	74.15	0.71			7.94	0.000	Low	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.7		8	72.54	1.57			7.64	0.009	High	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.7		8	73.80	0.82			7.63	0.006	Low	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.4		8	65.89	7.37			7.37	0.018	High	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.4		8	63.29	6.98			7.38	0.015	Low	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 8.1		17	78.20	2.87			8.03	0.003	High	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 8.1		17	80.37	1.33			8.03	0.013	Low	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.7		17	81.60	1.32			7.67	0.003	High	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.7		17	83.40	1.54			7.76	0.003	Low	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.4		17	72.73	6.68			7.38	0.006	High	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.4		17	79.23	2.66			7.38	0.003	Low	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 8.1		25	82.08	2.05			7.99	0.018	High	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 8.1		25	82.37	1.87			8.00	0.003	Low	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.7		25	90.16	0.87			7.67	0.023	High	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.7		25	86.99	2.20			7.67	0.017	Low	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.4		25	77.41	7.98			7.40	0.020	High	34.4	19.0
laboratory	Evechinus chloroticus (echinoderms)	414225	marinespecies.org	4	pH 7.4		25	86.66	3.35			7.37	0.003	Low	34.4	19.0