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Johnson, Maggie Dorothy; Carpenter, Robert C (2018): Seawater carbonate chemistry and net calcification, relative electron transport rates and photosynthetic pigments of Porolithon onkodes [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.924886

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Abstract:
Ocean acidification (OA) and nutrient enrichment threaten the persistence of near shore ecosystems, yet little is known about their combined effects on marine organisms. Here, we show that a threefold increase in nitrogen concentrations, simulating enrichment due to coastal eutrophication or consumer excretions, offset the direct negative effects of near-future OA on calcification and photophysiology of the reef-building crustose coralline alga, Porolithon onkodes. Projected near-future pCO2 levels (approx. 850 µatm) decreased calcification by 30% relative to ambient conditions. Conversely, nitrogen enrichment (nitrate + nitrite and ammonium) increased calcification by 90–130% in ambient and high pCO2 treatments, respectively. pCO2 and nitrogen enrichment interactively affected instantaneous photophysiology, with highest relative electron transport rates under high pCO2 and high nitrogen. Nitrogen enrichment alone increased concentrations of the photosynthetic pigments chlorophyll a, phycocyanin and phycoerythrin by approximately 80–450%, regardless of pCO2. These results demonstrate that nutrient enrichment can mediate direct organismal responses to OA. In natural systems, however, such direct benefits may be counteracted by simultaneous increases in negative indirect effects, such as heightened competition. Experiments exploring the effects of multiple stressors are increasingly becoming important for improving our ability to understand the ramifications of local and global change stressors in near shore ecosystems.
Keyword(s):
Animalia; Benthic animals; Benthos; Calcification/Dissolution; Cnidaria; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Macro-nutrients; Porolithon onkodes; Primary production/Photosynthesis; Single species; South Pacific; Tropical
Supplement to:
Johnson, Maggie Dorothy; Carpenter, Robert C (2018): Nitrogen enrichment offsets direct negative effects of ocean acidification on a reef-building crustose coralline alga. Biology Letters, 14(7), 20180371, https://doi.org/10.1098/rsbl.2018.0371
Original version:
Johnson, Maggie Dorothy; Carpenter, Robert C (2018): Calcification, photophysiology and treatment parameters for laboratory experiment. PANGAEA, https://doi.org/10.1594/PANGAEA.887917
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2020): seacarb: seawater carbonate chemistry with R. R package version 3.2.14. https://CRAN.R-project.org/package=seacarb
Coverage:
Latitude: -17.533000 * Longitude: 149.833000
Event(s):
Moorea_north_shore * Latitude: -17.533000 * Longitude: 149.833000 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2020) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-11-11.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
TypeTypeJohnson, Maggie Dorothystudy
SpeciesSpeciesJohnson, Maggie Dorothy
Registration number of speciesReg spec noJohnson, Maggie Dorothy
Uniform resource locator/link to referenceURL refJohnson, Maggie DorothyWoRMS Aphia ID
IdentificationIDJohnson, Maggie Dorothy
TreatmentTreatJohnson, Maggie DorothyACHN (Ambient CO2&High Nitrogen), ACAN (Ambient CO2&Ambient Nitrogen), HCHN (High CO2&High Nitrogen), HCAN (High CO2&Ambient nitrogen)
Calcification rateCalc ratemg/cm2Johnson, Maggie Dorothynet
Electron transport rate, relativerETRJohnson, Maggie Dorothy
Chlorophyll aChl aµg/mgJohnson, Maggie Dorothyper fresh weight
10 PhycocyaninPhycocµg/gJohnson, Maggie Dorothyper fresh weight
11 PhycoerythrinPhycoeµg/gJohnson, Maggie Dorothyper fresh weight
12 Temperature, waterTemp°CJohnson, Maggie Dorothy
13 Temperature, water, standard errorT std e±Johnson, Maggie Dorothy
14 SalinitySalJohnson, Maggie Dorothy
15 Salinity, standard errorSal std e±Johnson, Maggie Dorothy
16 IrradianceEµmol/m2/sJohnson, Maggie Dorothy
17 Irradiance, standard errorE std e±Johnson, Maggie Dorothy
18 Flow rateFlow ratel/minJohnson, Maggie Dorothy
19 Flow rate, standard errorFlow rate std e±Johnson, Maggie Dorothy
20 pHpHJohnson, Maggie Dorothytotal scale
21 pH, standard errorpH std e±Johnson, Maggie Dorothytotal scale
22 Alkalinity, totalATµmol/kgJohnson, Maggie Dorothy
23 Alkalinity, total, standard errorAT std e±Johnson, Maggie Dorothy
24 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmJohnson, Maggie DorothyCalculated using CO2SYS
25 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard errorpCO2water_SST_wet std e±Johnson, Maggie DorothyCalculated using CO2SYS
26 Calcite saturation stateOmega CalJohnson, Maggie DorothyCalculated using CO2SYS
27 Calcite saturation state, standard errorOmega Cal std e±Johnson, Maggie DorothyCalculated using CO2SYS
28 Nitrate and Nitrite[NO3]- + [NO2]-µmol/lJohnson, Maggie Dorothy
29 Nitrate and Nitrite, standard error[NO3]- + [NO2]- std e±Johnson, Maggie Dorothy
30 Ammonium[NH4]+µmol/lJohnson, Maggie Dorothy
31 Ammonium, standard error[NH4]+ std e±Johnson, Maggie Dorothy
32 Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33 Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36 Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37 Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
38 Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39 Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
40 Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
11905 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Type
(study)

Species

Reg spec no

URL ref
(WoRMS Aphia ID)

ID

Treat
(ACHN (Ambient CO2&High Nitrog...)

Calc rate [mg/cm2]
(net)

rETR

Chl a [µg/mg]
(per fresh weight)
10 
Phycoc [µg/g]
(per fresh weight)
11 
Phycoe [µg/g]
(per fresh weight)
12 
Temp [°C]
13 
T std e [±]
14 
Sal
15 
Sal std e [±]
16 
E [µmol/m2/s]
17 
E std e [±]
18 
Flow rate [l/min]
19 
Flow rate std e [±]
20 
pH
(total scale)
21 
pH std e [±]
(total scale)
22 
AT [µmol/kg]
23 
AT std e [±]
24 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)
25 
pCO2water_SST_wet std e [±]
(Calculated using CO2SYS)
26 
Omega Cal
(Calculated using CO2SYS)
27 
Omega Cal std e [±]
(Calculated using CO2SYS)
28 
[NO3]- + [NO2]- [µmol/l]
29 
[NO3]- + [NO2]- std e [±]
30 
[NH4]+ [µmol/l]
31 
[NH4]+ std e [±]
32 
CSC flag
(Calculated using seacarb afte...)
33 
CO2 [µmol/kg]
(Calculated using seacarb afte...)
34 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
35 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)
36 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)
37 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)
38 
DIC [µmol/kg]
(Calculated using seacarb afte...)
39 
Omega Arg
(Calculated using seacarb afte...)
40 
Omega Cal
(Calculated using seacarb afte...)
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN9.80619128.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN7.99726828.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN3.31334028.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN7.29002728.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN10.11899228.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN12.84678628.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN7.51628628.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN11.30164728.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN3.8915250.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN7.02702628.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN4.20713428.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN5.58294928.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org3ACHN2.62783028.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN6.96383528.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN6.55744028.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN5.70790228.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN4.77345628.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN10.61957128.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN10.54338828.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN6.71049328.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN2.19017428.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN28.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN8.30275028.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN9.67968028.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN6.57686028.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org4HCHN3.29194028.30.0436.10.0832980.13827.790.01323295800263.660.090.840.0822.451.55820.82799.54802.001958.83151.032130.682.423.62
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN5.10005728.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN3.93845328.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN2.01291828.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN-3.31600928.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN5.36212528.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN2.15242228.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN2.77131528.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN7.88349028.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN7.99726828.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN4.64246128.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN6.57686028.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN2.42210728.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org5ACAN6.08013828.40.0736.10.07334160.13927.990.0032342746445.300.020.660.026.340.61812.05463.86465.291800.14220.782032.973.535.30
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN5.07005628.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN0.00000028.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN6.96394128.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN7.44393228.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN-0.87045128.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN3.68670828.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN2.26014528.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN3.78471528.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN-1.92745428.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN1.33918628.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN2.27661628.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN0.48868028.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org6HCAN3.06407128.50.0736.20.08336130.14027.780.01723382843343.620.120.770.096.050.56821.35823.88826.411970.80149.912142.062.403.59
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org9ACHN7.60198828.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org9ACHN9.13986328.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org9ACHN7.50996128.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org9ACHN6.60348828.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org9ACHN10.95203128.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org9ACHN8.62414728.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org9ACHN5.57097528.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
laboratoryPorolithon onkodes (macroalga)495983marinespecies.org9ACHN6.31076928.40.0336.10.06329130.13828.000.0062326445485.320.050.990.0519.082.27811.63447.64449.011777.63223.092012.363.575.35
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