Peterse, Francien; van Bree, Loes G J; Baxter, Allix J; De Crop, Wannes; van Grinsven, Sigrid; Villanueva, Laura; Verschuren, Dirk; Sinninghe Damsté, Jaap S (2020): Bacterial community composition in suspended particulate matter of Lake Chala from September 2013- January 2015 [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.924859
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Abstract:
Vertical profiles of temperature, dissolved oxygen (DO), conductivity and pH in the water column of Lake Chala, a small (4.2 km2), deep (~90 m), and permanently stratified tropical crater lake at ~880 m above sea level in East Africa (3°19'S, 37°42'E), were measured at 2 m intervals through the upper 50 m of the water column at monthly intervals between September 2013 and January 2015. In the same time interval, suspended particulate matter (SPM) was collected monthly at 13 discrete depths by filtering 5-10L of water over pre-combusted glass fibre filters. A 1/32 section of the same SPM filters as used for brGDGT analysis was used for DNA extraction, after which 16S rRNA gene amplicon sequencing and quantification was performed to generate concentration-depth profiles of the monthly distribution and abundance of bacterial taxa.
Related to:
van Bree, Loes G J; Peterse, Francien; Baxter, Allix J; De Crop, Wannes; van Grinsven, Sigrid; Villanueva, Laura; Verschuren, Dirk; Sinninghe Damsté, Jaap S (2020): Seasonal variability and sources of in situ brGDGT production in a permanently stratified African crater lake. Biogeosciences, 17(21), 5443-5463, https://doi.org/10.5194/bg-17-5443-2020
Coverage:
Latitude: -3.316670 * Longitude: 37.700000
Date/Time Start: 2010-08-31T00:00:00 * Date/Time End: 2015-01-31T00:00:00
Minimum Elevation: 880.0 m * Maximum Elevation: 880.0 m
Event(s):
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
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1 | Binary Object | Binary | Peterse, Francien | |||
2 | Binary Object (Media Type) | Binary (Type) | Peterse, Francien | |||
3 | File content | Content | Peterse, Francien |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
2 data points
Data
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1 Binary | 2 Binary (Type) | 3 Content |
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16SrRNA_genes.xlsx | application/vnd.openxmlformats-officedocument.spreadsheetml.sheet | Relative abundance of 16S rRNA gene copies (in %) up to taxonomic level L3 obtained by the 16S rRNA gene amplicon sequencing and quantitative PCR for all Acidobacteria SDs and the 12 additional bacterial species that are included in Fig. 8 of the associated publication (van Bree et al., 2020). |