Citation:

PANGAEA.924085

Name: Seawater carbonate chemistry and physiological performance of the rhodolith Sporolithon sp.
Published: 2020-10-26
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Macroalgae; North Pacific; Plantae; Rhodophyta; Single species; Sporolithon sp.; Tropical

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Abstract:

Fish farming in coastal areas has become an important source of food to support the world's increasing population. However, intensive and unregulated mariculture activities have contributed to changing seawater carbonate chemistry through the production of high levels of respiratory CO2. This additional CO2, i.e. in addition to atmospheric inputs, intensifies the effects of global ocean acidification resulting in localized extreme low pH levels. Marine calcifying macroalgae are susceptible to such changes due to their CaCO3 skeleton. Their physiological response to CO2-driven acidification is dependent on their carbon physiology. In this study, we used the pH drift experiment to determine the capability of 9 calcifying macroalgae to use one or more inorganic carbon (Ci) species. From the 9 species, we selected the rhodolith Sporolithon sp. as a model organism to investigate the long-term effects of extreme low pH on the physiology and biochemistry of calcifying macroalgae. Samples were incubated under two pH treatments (pH 7.9 = ambient and pH 7.5 = extreme acidification) in a temperature-controlled (26 ± 0.02 °C) room provided with saturating light intensity (98.3 ± 2.50 μmol photons/m**2/s). After the experimental treatment period (40 d), growth rate, calcification rate, nutrient uptake rate, organic content, skeletal CO3-2, pigments, and tissue C, N and P of Sporolithon samples were compared. The pH drift experiment revealed species-specific Ci use mechanisms, even between congenerics, among tropical calcifying macroalgae. Furthermore, long-term extreme low pH significantly reduced the growth rate, calcification rate and skeletal CO3-2 content by 79%, 66% and 18%, respectively. On the other hand, nutrient uptake rates, organic matter, pigments and tissue C, N and P were not affected by the low pH treatments. Our results suggest that the rhodolith Sporolithon sp. is susceptible to the negative effects of extreme low pH resulting from intensive mariculture-driven coastal acidification.

Keyword(s):

Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Macroalgae; North Pacific; Plantae; Rhodophyta; Single species; Sporolithon sp.; Tropical

Supplement to:

Narvarte, Bienson Ceasar V; Nelson, Wendy A; Roleda, Michael Y (2020): Inorganic carbon utilization of tropical calcifying macroalgae and the impacts of intensive mariculture-derived coastal acidification on the physiological performance of the rhodolith Sporolithon sp. Environmental Pollution, 266, 115344, https://doi.org/10.1016/j.envpol.2020.115344

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 16.428600 * Longitude: 119.930500

Event(s):

Dos_Hermanos * Latitude: 16.428600 * Longitude: 119.930500 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-10-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Narvarte, Bienson Ceasar V		study
2	Species	Species		Narvarte, Bienson Ceasar V
3	Treatment	Treat		Narvarte, Bienson Ceasar V
4	Growth	Growth	%	Narvarte, Bienson Ceasar V
5	Growth, relative, standard error	Growth rel std e	±	Narvarte, Bienson Ceasar V
6	Calcification rate of calcium carbonate	Calc rate CaCO3	µmol/g/day	Narvarte, Bienson Ceasar V
7	Calcification rate, standard error	Calc rate std e	±	Narvarte, Bienson Ceasar V
8	Inorganic matter	IM	%	Narvarte, Bienson Ceasar V
9	Inorganic matter, standard error	IM std e	±	Narvarte, Bienson Ceasar V
10	Skeleton	Skel	%	Narvarte, Bienson Ceasar V		%CO3
11	Skeleton, standard error	Skeleton std e	±	Narvarte, Bienson Ceasar V
12	Organic matter	OM	%	Narvarte, Bienson Ceasar V
13	Organic matter, standard error	OM std e	±	Narvarte, Bienson Ceasar V
14	Chlorophyll a	Chl a	µg/g	Narvarte, Bienson Ceasar V
15	Chlorophyll a, standard error	Chl a std e	±	Narvarte, Bienson Ceasar V
16	Chlorophyll d	Chl d	µg/g	Narvarte, Bienson Ceasar V
17	Chlorophyll d, standard error	Chl d std e	±	Narvarte, Bienson Ceasar V
18	Phycocyanin	Phycoc	µg/g	Narvarte, Bienson Ceasar V
19	Phycocyanin, standard error	Phycoc std e	±	Narvarte, Bienson Ceasar V
20	Allophycocyanin	APC	mg/g	Narvarte, Bienson Ceasar V
21	Allophycocyanin, standard error	APC std e	±	Narvarte, Bienson Ceasar V
22	Phycoerythrin	Phycoe	µg/g	Narvarte, Bienson Ceasar V
23	Phycoerythrin, standard error	Phycoe std e	±	Narvarte, Bienson Ceasar V
24	Ammonium uptake rate	[NH4+] upt rate	µmol/g/day	Narvarte, Bienson Ceasar V
25	Ammonium uptake rate, standard error	[NH4]+ upt rate std e	±	Narvarte, Bienson Ceasar V
26	Nitrite uptake rate	[NO2]- upt rate	µmol/g/day	Narvarte, Bienson Ceasar V
27	Nitrite uptake rate, standard error	[NO2]- upt rate std e	±	Narvarte, Bienson Ceasar V
28	Nitrate uptake rate	NO3 upt rate	µmol/g/day	Narvarte, Bienson Ceasar V
29	Nitrate uptake rate, standard error	NO3 upt rate std e	±	Narvarte, Bienson Ceasar V
30	Phosphate uptake rate	PO4 upt rate	µmol/g/day	Narvarte, Bienson Ceasar V
31	Phosphate uptake rate, standard error	PO4 upt rate std e	±	Narvarte, Bienson Ceasar V
32	Carbon	C	%	Narvarte, Bienson Ceasar V		tissue
33	Carbon, standard error	C std e	±	Narvarte, Bienson Ceasar V		tissue
34	Nitrogen	N	%	Narvarte, Bienson Ceasar V		tissue
35	Nitrogen, standard error	N std e	±	Narvarte, Bienson Ceasar V		tissue
36	Phosphorus	P	%	Narvarte, Bienson Ceasar V		tissue
37	Phosphorus, standard error	P std e	±	Narvarte, Bienson Ceasar V		tissue
38	Carbon/Nitrogen ratio	C/N		Narvarte, Bienson Ceasar V		tissue
39	Carbon/Nitrogen ratio, standard error	C/N std e	±	Narvarte, Bienson Ceasar V		tissue
40	Carbon/Phosphorus ratio	C/P		Narvarte, Bienson Ceasar V		tissue
41	Carbon/Phosphorus ratio, standard error	C/P std e	±	Narvarte, Bienson Ceasar V		tissue
42	Nitrogen/Phosphorus ratio	N/P		Narvarte, Bienson Ceasar V		tissue
43	Nitrogen/Phosphorus ratio, standard error	N/P std e	±	Narvarte, Bienson Ceasar V		tissue
44	pH	pH		Narvarte, Bienson Ceasar V	Potentiometric	total scale
45	pH, standard error	pH std e	±	Narvarte, Bienson Ceasar V	Potentiometric	total scale
46	Carbon dioxide	CO2	µmol/kg	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
47	Carbon dioxide, standard error	CO2 std e	±	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
48	Bicarbonate ion	[HCO3]-	µmol/kg	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
49	Bicarbonate ion, standard error	[HCO3]- std e	±	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
50	Carbonate ion	[CO3]2-	µmol/kg	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
51	Carbonate ion, standard error	[CO3]2- std e	±	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
52	Carbon, inorganic, dissolved	DIC	µmol/kg	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
53	Carbon, inorganic, dissolved, standard error	DIC std e	±	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
54	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
55	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
56	Calcite saturation state	Omega Cal		Narvarte, Bienson Ceasar V	Calculated using CO2SYS
57	Calcite saturation state, standard error	Omega Cal std e	±	Narvarte, Bienson Ceasar V	Calculated using CO2SYS
58	Alkalinity, total	AT	µmol/kg	Narvarte, Bienson Ceasar V	Potentiometric titration
59	Alkalinity, total, standard error	AT std e	±	Narvarte, Bienson Ceasar V	Potentiometric titration
60	Salinity	Sal		Narvarte, Bienson Ceasar V
61	Salinity, standard error	Sal std e	±	Narvarte, Bienson Ceasar V
62	Temperature, water	Temp	°C	Narvarte, Bienson Ceasar V
63	Temperature, water, standard error	T std e	±	Narvarte, Bienson Ceasar V
64	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
65	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
66	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
67	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
68	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
69	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
70	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
71	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
72	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

144 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Treat	4 Growth [%]	5 Growth rel std e [±]	6 Calc rate CaCO3 [µmol/g/day]	7 Calc rate std e [±]	8 IM [%]	9 IM std e [±]	10 Skel [%] (%CO3)	11 Skeleton std e [±]	12 OM [%]	13 OM std e [±]	14 Chl a [µg/g]	15 Chl a std e [±]	16 Chl d [µg/g]	17 Chl d std e [±]	18 Phycoc [µg/g]	19 Phycoc std e [±]	20 APC [mg/g]	21 APC std e [±]	22 Phycoe [µg/g]	23 Phycoe std e [±]	24 [NH4+] upt rate [µmol/g/day]	25 [NH4]+ upt rate std e [±]	26 [NO2]- upt rate [µmol/g/day]	27 [NO2]- upt rate std e [±]	28 NO3 upt rate [µmol/g/day]	29 NO3 upt rate std e [±]	30 PO4 upt rate [µmol/g/day]	31 PO4 upt rate std e [±]	32 C [%] (tissue)	33 C std e [±] (tissue)	34 N [%] (tissue)	35 N std e [±] (tissue)	36 P [%] (tissue)	37 P std e [±] (tissue)	38 C/N (tissue)	39 C/N std e [±] (tissue)	40 C/P (tissue)	41 C/P std e [±] (tissue)	42 N/P (tissue)	43 N/P std e [±] (tissue)	44 pH (total scale, Potentiometric)	45 pH std e [±] (total scale, Potentiometric)	46 CO2 [µmol/kg] (Calculated using CO2SYS)	47 CO2 std e [±] (Calculated using CO2SYS)	48 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	49 [HCO3]- std e [±] (Calculated using CO2SYS)	50 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	51 [CO3]2- std e [±] (Calculated using CO2SYS)	52 DIC [µmol/kg] (Calculated using CO2SYS)	53 DIC std e [±] (Calculated using CO2SYS)	54 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	55 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	56 Omega Cal (Calculated using CO2SYS)	57 Omega Cal std e [±] (Calculated using CO2SYS)	58 AT [µmol/kg] (Potentiometric titration)	59 AT std e [±] (Potentiometric titration)	60 Sal	61 Sal std e [±]	62 Temp [°C]	63 T std e [±]	64 CSC flag (Calculated using seacarb afte...)	65 CO2 [µmol/kg] (Calculated using seacarb afte...)	66 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	67 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	68 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	69 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	70 DIC [µmol/kg] (Calculated using seacarb afte...)	71 Omega Arg (Calculated using seacarb afte...)	72 Omega Cal (Calculated using seacarb afte...)
laboratory	Sporolithon sp. (macroalga)	pH=7.9	0.110	0.01	12.30	0.79	23.8	1.19	32.2	1.62	15.6	1.47	35.5	2.87	0.84	0.51	70	10	0.23	0.03	120	20	0.12	0.01	0.07	0.01	0.04	0.004	0.09	0.01	13.6	0.29	0.55	0.11	0.04	0.012	27.5	5.85	13.3	4.40	0.55	0.22	7.93	0.01	10.2	0.5	1330	35.2	136.0	3.8	1480	36.1	360	17.9	2.15	0.1	1690	35.3	35	0.3	26	0.02	8	10.95	395.54	396.80	1351.18	129.02	1491.15	2.06	3.11
laboratory	Sporolithon sp. (macroalga)	pH=7.5	0.023	0.01	4.18	0.94	19.4	0.44	26.4	0.60	15.7	0.62	38.6	4.39	0.77	0.26	50	8	0.22	0.01	100	10	0.11	0.02	0.06	0.01	0.03	0.003	0.08	0.01	13.6	0.24	1.48	0.80	0.04	0.004	28.4	3.56	10.5	1.67	1.33	1.00	7.54	0.01	34.7	1.5	1890	56.2	80.7	3.6	2010	59.6	1230	52.7	1.27	0.1	2100	61.3	35	0.3	26	0.02	8	38.10	1375.75	1380.11	1914.51	74.47	2027.08	1.19	1.80