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Busch, Kathrin; Taboada, Sergi; Riesgo, Ana; Koutsouveli, Vasiliki; Rios, Pilar; Cristobo, Javier; Franke, Andre; Getzlaff, Klaus; Schmidt, Christina; Biastoch, Arne; Hentschel, Ute (2020): Metadata, flow cytometry data, and NCBI-Accession numbers (16S, 18S, COI, and SNP data) for fan-shaped sponges and seawater from the Cantabrian Sea in summer 2017. PANGAEA, https://doi.org/10.1594/PANGAEA.923271

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Abstract:
Connectivity is a fundamental process driving the persistence of marine populations and their adaptation potential in response to environmental change. In this study, we analysed the population genetics of two morphologically highly similar deep-sea sponge clades (Phakellia hirondellei and the 'Topsentia-and-Petromica (TaP)' clade) at three locations in the Cantabrian Sea. Sponge taxonomy was assessed by spicule analyses, as well as by 18S sequencing and COI sequencing. The corresponding host microbiome was analysed by 16S rRNA gene sequencing. In addition we set up an oceanographic modelling framework, for which we used seawater flow cytometry data (derived from bottom depths of CTD casts) as ground-truthing data.
Keyword(s):
amplicon sequencing; flow cytometry; population genetics; Porifera; single-nucleotide polymorphisms (SNPs)
Supplement to:
Busch, Kathrin; Taboada, Sergi; Riesgo, Ana; Koutsouveli, Vasiliki; Rios, Pilar; Cristobo, Javier; Franke, Andre; Getzlaff, Klaus; Schmidt, Christina; Biastoch, Arne; Hentschel, Ute (2021): Population connectivity of fan-shaped sponge holobionts in the deep Cantabrian Sea. Deep Sea Research Part I: Oceanographic Research Papers, 167, 103427, https://doi.org/10.1016/j.dsr.2020.103427
Funding:
Horizon 2020 (H2020), grant/award no. 679849: Deep-sea Sponge Grounds Ecosystems of the North Atlantic
Coverage:
Median Latitude: 43.993310 * Median Longitude: -5.513115 * South-bound Latitude: 43.772200 * West-bound Longitude: -6.473700 * North-bound Latitude: 44.421700 * East-bound Longitude: -4.637200
Date/Time Start: 2017-06-12T10:00:52 * Date/Time End: 2017-06-23T17:57:18
Minimum DEPTH, water: 303 m * Maximum DEPTH, water: 1522 m
Event(s):
SPONGES_0617_04-DR4 (DR4) * Latitude: 43.871900 * Longitude: -5.901800 * Date/Time: 2017-06-12T10:00:52 * Elevation: -695.0 m * Location: Bay of Biscay * Campaign: SponGES_0617 * Basis: Angeles Alvarino * Method/Device: Dredge, rock (DRG_R)
SPONGES_0617_07-CTD1 (CTD1) * Latitude: 43.918800 * Longitude: -6.265500 * Date/Time: 2017-06-13T11:30:31 * Elevation: -1172.0 m * Location: Bay of Biscay * Campaign: SponGES_0617 * Basis: Angeles Alvarino * Method/Device: CTD/Rosette (CTD-RO)
SPONGES_0617_12-CTD2 (CTD2) * Latitude: 43.776800 * Longitude: -6.203700 * Date/Time: 2017-06-14T11:04:40 * Elevation: -1152.0 m * Location: Bay of Biscay * Campaign: SponGES_0617 * Basis: Angeles Alvarino * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventBusch, Kathrin
2Sample code/labelSample labelBusch, Kathrin
3Sample IDSample IDBusch, Kathrinonboard
4Date/Time of eventDate/TimeBusch, Kathrin
5Latitude of eventLatitudeBusch, Kathrin
6Longitude of eventLongitudeBusch, Kathrin
7DEPTH, waterDepth watermBusch, KathrinGeocode
8Method/Device of eventMethod/DeviceBusch, Kathrin
9Area/localityAreaBusch, Kathrin
10Geology, commentGeo commBusch, Kathringeologic setting
11Accession number, geneticsAccess no genBusch, Kathrin16S
12Accession number, geneticsAccess no genBusch, Kathrin18S
13Accession number, geneticsAccess no genBusch, KathrinCOI
14Accession number, geneticsAccess no genBusch, KathrinSNPs
15Measurement conductedMeasurementBusch, KathrinSpicule measurements
16Measurement conductedMeasurementBusch, Kathrinsterol measurements
17Measurement conductedMeasurementBusch, Kathrinmicroscopy_TEM
18BacteriaBact#/mlBusch, KathrinFlow cytometry
19PhytoplanktonPhytopl#/lBusch, KathrinFlow cytometry
Size:
550 data points

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