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Reintjes, Greta; Amann, Rudolf; Arnosti, Carol; Fuchs, Bernhard M (2020): Microbial community composition and polysaccharide processing potential in the South Pacific Gyre. PANGAEA, https://doi.org/10.1594/PANGAEA.922868

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Abstract:
The South Pacific Gyre (SPG) covers 10% of the ocean's surface and is considered a marine biological desert. However, recent investigations have shown that primary production occurs throughout its deep euphotic zone and that this fuels the regeneration of nutrients and the recycling of organic matter. We set out to investigate the SPG's microbial communities' heterotrophic capability to utilize polysaccharides, an important marine organic matter component. Using fluorescently labeled polysaccharide (FLA-PS) incubations (Reintjes, et al., 2017), we analyzed the initial step of organic matter degradation by measuring both the rate of external hydrolysis and the rate of direct uptake of polysaccharides by marine microorganisms. Furthermore, we investigated the change in bacterial abundance and diversity during the FLA-PS incubations using direct cell counts and 16S rRNA sequencing. The presented dataset contains the microbial diversity, total cellular abundance, and direct FLA-PS uptake results generated during the FLA-PS incubations performed with six polysaccharides (laminarin, xylan, chondroitin sulfate, arabinogalactan, fucoidan, and pullulan) over 18 days. The incubations were performed with seawater from the epipelagic and bathypelagic (75 m, 160 m, 1250 m, and 2800 m) in the central gyre, and seawater from the epipelagic (75 m) at two stations adjacent to the gyre. Our study found that the SPG's microbial community showed remarkably high extracellular enzyme activities, and a considerable fraction of the microorganisms were capable of the direct uptake (selfish-uptake) of FLA-PS. Interestingly, a wide variety of bacteria were capable of cycling HMW organic matter using distinct polysaccharide processing mechanisms in the SPG. This research shows that the SPG features not only organisms capable of existing on the fine edge of minimal substrate concentrations but also those capable of taking advantage of abrupt changes in physical conditions and substrate availability
Keyword(s):
activity; FLA-PS; Gyre; heterotrophy; metabolism; microbial; Pacific; polysaccharide; SO245
Supplement to:
Reintjes, Greta; Fuchs, Bernhard M; Amann, Rudolf; Arnosti, Carol (2020): Extensive microbial processing of polysaccharides in the South Pacific Gyre via selfish uptake and extracellular hydrolysis. Frontiers in Microbiology, 11, https://doi.org/10.3389/fmicb.2020.583158
Related to:
Reintjes, Greta; Amann, Rudolf I; Arnosti, Carol; Fuchs, Bernhard M (2020): Microbial community composition and polysaccharide processing potential in the South Pacific Gyre. European Nucleotide Archive (ENA), insdc:PRJEB40503
Reintjes, Greta; Arnosti, Carol; Fuchs, Bernhard M; Amann, Rudolf I (2017): An alternative polysaccharide uptake mechanism of marine bacteria. The ISME Journal, 11, 1640-1650
Coverage:
Median Latitude: -24.910977 * Median Longitude: -105.899390 * South-bound Latitude: -27.740930 * West-bound Longitude: -117.618850 * North-bound Latitude: -23.491900 * East-bound Longitude: -90.029850
Date/Time Start: 2015-12-27T07:25:00 * Date/Time End: 2016-01-06T21:40:00
Minimum DEPTH, water: 75 m * Maximum DEPTH, water: 2800 m
Event(s):
SO245_2-2 * Latitude: -23.491900 * Longitude: -90.029850 * Date/Time: 2015-12-27T07:25:00 * Elevation: -3890.9 m * Location: South Pacific Ocean * Campaign: SO245 (UltraPac, GEOTRACES) * Basis: Sonne_2 * Method/Device: CTD/Rosette (CTD-RO)
SO245_6-1 * Latitude: -23.500100 * Longitude: -110.049470 * Date/Time: 2016-01-03T09:21:00 * Elevation: -3612.8 m * Location: South Pacific Ocean * Campaign: SO245 (UltraPac, GEOTRACES) * Basis: Sonne_2 * Method/Device: CTD/Rosette (CTD-RO)
SO245_8-1 * Latitude: -27.740930 * Longitude: -117.618850 * Date/Time: 2016-01-06T21:40:00 * Elevation: -3697.5 m * Location: South Pacific Ocean * Campaign: SO245 (UltraPac, GEOTRACES) * Basis: Sonne_2 * Method/Device: CTD/Rosette (CTD-RO)
Comment:
<0.01 = n.a.
Hydrolysis rates were measured as the change in polysaccharide molecular weight as a function of time, as determined via gel permeation chromatography with fluorescence detection (Arnosti, 2003; doi:10.3389/fmicb.2020.583158). Unit: rate.x.nM.L.hr (x being the individual substrate, highlighted in column 11 - Substrate Type).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventReintjes, Greta
2Date/Time of eventDate/TimeReintjes, Greta
3Latitude of eventLatitudeReintjes, Greta
4Longitude of eventLongitudeReintjes, Greta
5Location of eventLocationReintjes, Greta
6Campaign of eventCampaignReintjes, Greta
7Basis of eventBasisReintjes, Greta
8Sample IDSample IDReintjes, Greta
9DEPTH, waterDepth watermReintjes, GretaGeocode
10Sample methodSample methodReintjes, Greta
11Substrate typeSubstrateReintjes, Greta
12Day of experimentDOEdayReintjes, Greta
13VolumeVolmlReintjes, Greta
14Microbial abundance, cellsMicrobial abund#/mlReintjes, GretaFluorescence in situ hybridization (FISH)
15Microbial abundance, cellsMicrobial abund#/mlReintjes, GretaFluorescence in situ hybridization (FISH)Of substrate stained cells
16Extracellular Enzyme ActivityEEAarbitrary unitsArnosti, CarolFluorescently labeled polysaccharides (FLA-PS)[rate.x.nM.L.hr]
17Extracellular Enzyme ActivityEEAarbitrary unitsArnosti, CarolFluorescently labeled polysaccharides (FLA-PS)[rate.1.nM.L.hr]
18Extracellular Enzyme ActivityEEAarbitrary unitsArnosti, CarolFluorescently labeled polysaccharides (FLA-PS)[rate.2.nM.L.hr]
19Extracellular Enzyme ActivityEEAarbitrary unitsArnosti, CarolFluorescently labeled polysaccharides (FLA-PS)[rate.3.nM.L.hr]
20Extracellular Enzyme ActivityEEAarbitrary unitsArnosti, CarolFluorescently labeled polysaccharides (FLA-PS)[mean.rate.nM.L.hr]
21Extracellular Enzyme ActivityEEAarbitrary unitsArnosti, CarolFluorescently labeled polysaccharides (FLA-PS)[sd.rate.nM.L.hr]
Size:
1618 data points

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