Citation:

PANGAEA.922623

Name: Seawater carbonate chemistry and hatching success of anemonefish
Published: 2020-09-11
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Amphiprion sebae; Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Indian Ocean; Laboratory experiment; Nekton; Pelagos; Reproduction; Single species; Tropical

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Abstract:

Oceans have continuously absorbed anthropogenic carbon dioxide from the atmosphere. The early life stages are likely vulnerable to low pH conditions. The present study aimed to assess the anemonefish (Amphiprionsebae), egg hatch rate and gonadal tissue condition in different pH levels in 6 weeks. Seawater pH was manually manipulated by bubbling known concentrations of CO2 to achieve three pH treatments 8.1, 7.7 and 7.3. Egg hatch rate decreased with declining pH (ambient pH – 93 %, pH 7.7 – 92 %, pH 7.3 – 88 %) and a slight time delay was observed between the ambient and lower pH treatments. The condition of testicular and ovarian tissue was not affected by low pH levels. Overall, present experiment found that the slight negative effects on egg hatching success in low pH condition. The experimental results suggested that early life stages expected to vulnerable to near future ocean acidification.

Keyword(s):

Amphiprion sebae; Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Indian Ocean; Laboratory experiment; Nekton; Pelagos; Reproduction; Single species; Tropical

Supplement to:

Kannan, Gunasekaran; Ayyappan, Saravanakumar; Mariasingarayan, Yosuva (2020): Ocean Acidification Impacts on Hatching Success and Reproductive Tissue Damage in Anemonefish. Indian Journal of Natural Sciences, 10, 17992-18000

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Kannan, Gunasekaran		study
2	Species	Species		Kannan, Gunasekaran
3	Registration number of species	Reg spec no		Kannan, Gunasekaran
4	Uniform resource locator/link to reference	URL ref		Kannan, Gunasekaran		WoRMS Aphia ID
5	Treatment	Treat		Kannan, Gunasekaran
6	Identification	ID		Kannan, Gunasekaran
7	Hatching rate	Hatching	%	Kannan, Gunasekaran
8	pH	pH		Kannan, Gunasekaran	Potentiometric	total scale
9	pH, standard deviation	pH std dev	±	Kannan, Gunasekaran	Potentiometric	total scale
10	Temperature, water	Temp	°C	Kannan, Gunasekaran
11	Temperature, water, standard deviation	Temp std dev	±	Kannan, Gunasekaran
12	Salinity	Sal		Kannan, Gunasekaran
13	Salinity, standard deviation	Sal std dev	±	Kannan, Gunasekaran
14	Alkalinity, total	AT	µmol/kg	Kannan, Gunasekaran	Potentiometric titration
15	Alkalinity, total, standard deviation	AT std dev	±	Kannan, Gunasekaran	Potentiometric titration
16	Carbon, inorganic, dissolved	DIC	µmol/kg	Kannan, Gunasekaran	Calculated using CO2SYS
17	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Kannan, Gunasekaran	Calculated using CO2SYS
18	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Kannan, Gunasekaran	Calculated using CO2SYS
19	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Kannan, Gunasekaran	Calculated using CO2SYS
20	Bicarbonate ion	[HCO3]-	µmol/kg	Kannan, Gunasekaran	Calculated using CO2SYS
21	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Kannan, Gunasekaran	Calculated using CO2SYS
22	Carbonate ion	[CO3]2-	µmol/kg	Kannan, Gunasekaran	Calculated using CO2SYS
23	Carbonate ion, standard deviation	[CO3]2- std dev	±	Kannan, Gunasekaran	Calculated using CO2SYS
24	Calcite saturation state	Omega Cal		Kannan, Gunasekaran	Calculated using CO2SYS
25	Calcite saturation state, standard deviation	Omega Cal std dev	±	Kannan, Gunasekaran	Calculated using CO2SYS
26	Aragonite saturation state	Omega Arg		Kannan, Gunasekaran	Calculated using CO2SYS
27	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Kannan, Gunasekaran	Calculated using CO2SYS
28	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
31	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
35	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
37	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
39	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
41	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
43	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1188 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 ID	7 Hatching [%]	8 pH (total scale, Potentiometric)	9 pH std dev [±] (total scale, Potentiometric)	10 Temp [°C]	11 Temp std dev [±]	12 Sal	13 Sal std dev [±]	14 AT [µmol/kg] (Potentiometric titration)	15 AT std dev [±] (Potentiometric titration)	16 DIC [µmol/kg] (Calculated using CO2SYS)	17 DIC std dev [±] (Calculated using CO2SYS)	18 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	19 pCO2 std dev [±] (Calculated using CO2SYS)	20 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	21 [HCO3]- std dev [±] (Calculated using CO2SYS)	22 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	23 [CO3]2- std dev [±] (Calculated using CO2SYS)	24 Omega Cal (Calculated using CO2SYS)	25 Omega Cal std dev [±] (Calculated using CO2SYS)	26 Omega Arg (Calculated using CO2SYS)	27 Omega Arg std dev [±] (Calculated using CO2SYS)	28 CSC flag (Calculated using seacarb afte...)	29 CO2 [µmol/kg] (Calculated using seacarb afte...)	30 CO2 std dev [±] (Calculated using seacarb afte...)	31 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	32 fCO2 std dev [±] (Calculated using seacarb afte...)	33 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	34 pCO2 std dev [±] (Calculated using seacarb afte...)	35 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	36 [HCO3]- std dev [±] (Calculated using seacarb afte...)	37 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	38 [CO3]2- std dev [±] (Calculated using seacarb afte...)	39 DIC [µmol/kg] (Calculated using seacarb afte...)	40 DIC std dev [±] (Calculated using seacarb afte...)	41 Omega Arg (Calculated using seacarb afte...)	42 Omega Arg std dev [±] (Calculated using seacarb afte...)	43 Omega Cal (Calculated using seacarb afte...)	44 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 8.1	Clutch 1	94.70	8.14	0.21	27	2.4	33	2	2495.05	8.95	2105.14	24.04	325.07	21.12	1812.33	25.57	283.96	14.19	6.97	1.56	4.60	0.98	8	9.10	5.63	333.30	205.04	334.34	205.68	1823.31	244.38	279.44	100.87	2111.85	149.29	4.54	1.66	6.87	2.50
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 8.1	Clutch 2	93.30	8.14	0.21	27	2.4	33	2	2495.05	8.95	2105.14	24.04	325.07	21.12	1812.33	25.57	283.96	14.19	6.97	1.56	4.60	0.98	8	9.10	5.63	333.30	205.04	334.34	205.68	1823.31	244.38	279.44	100.87	2111.85	149.29	4.54	1.66	6.87	2.50
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 8.1	Clutch 3	89.90	8.14	0.21	27	2.4	33	2	2495.05	8.95	2105.14	24.04	325.07	21.12	1812.33	25.57	283.96	14.19	6.97	1.56	4.60	0.98	8	9.10	5.63	333.30	205.04	334.34	205.68	1823.31	244.38	279.44	100.87	2111.85	149.29	4.54	1.66	6.87	2.50
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 8.1	Clutch 4	95.20	8.14	0.21	27	2.4	33	2	2495.05	8.95	2105.14	24.04	325.07	21.12	1812.33	25.57	283.96	14.19	6.97	1.56	4.60	0.98	8	9.10	5.63	333.30	205.04	334.34	205.68	1823.31	244.38	279.44	100.87	2111.85	149.29	4.54	1.66	6.87	2.50
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 8.1	Clutch 5	93.10	8.14	0.21	27	2.4	33	2	2495.05	8.95	2105.14	24.04	325.07	21.12	1812.33	25.57	283.96	14.19	6.97	1.56	4.60	0.98	8	9.10	5.63	333.30	205.04	334.34	205.68	1823.31	244.38	279.44	100.87	2111.85	149.29	4.54	1.66	6.87	2.50
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 8.1	Clutch 6	95.80	8.14	0.21	27	2.4	33	2	2495.05	8.95	2105.14	24.04	325.07	21.12	1812.33	25.57	283.96	14.19	6.97	1.56	4.60	0.98	8	9.10	5.63	333.30	205.04	334.34	205.68	1823.31	244.38	279.44	100.87	2111.85	149.29	4.54	1.66	6.87	2.50
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 8.1	Clutch 7	94.10	8.14	0.21	27	2.4	33	2	2495.05	8.95	2105.14	24.04	325.07	21.12	1812.33	25.57	283.96	14.19	6.97	1.56	4.60	0.98	8	9.10	5.63	333.30	205.04	334.34	205.68	1823.31	244.38	279.44	100.87	2111.85	149.29	4.54	1.66	6.87	2.50
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 8.1	Clutch 8	92.70	8.14	0.21	27	2.4	33	2	2495.05	8.95	2105.14	24.04	325.07	21.12	1812.33	25.57	283.96	14.19	6.97	1.56	4.60	0.98	8	9.10	5.63	333.30	205.04	334.34	205.68	1823.31	244.38	279.44	100.87	2111.85	149.29	4.54	1.66	6.87	2.50
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 8.1	Clutch 9	95.20	8.14	0.21	27	2.4	33	2	2495.05	8.95	2105.14	24.04	325.07	21.12	1812.33	25.57	283.96	14.19	6.97	1.56	4.60	0.98	8	9.10	5.63	333.30	205.04	334.34	205.68	1823.31	244.38	279.44	100.87	2111.85	149.29	4.54	1.66	6.87	2.50
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.7	Clutch 1	93.18	7.73	0.14	27	2.3	33	2	2473.19	15.48	2316.09	28.12	994.69	19.58	2157.51	15.48	131.51	22.76	3.23	1.05	2.13	0.75	8	27.75	10.21	1016.58	369.67	1019.76	370.82	2163.59	92.88	129.00	38.17	2320.34	65.51	2.09	0.63	3.17	0.95
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.7	Clutch 2	93.50	7.73	0.14	27	2.3	33	2	2473.19	15.48	2316.09	28.12	994.69	19.58	2157.51	15.48	131.51	22.76	3.23	1.05	2.13	0.75	8	27.75	10.21	1016.58	369.67	1019.76	370.82	2163.59	92.88	129.00	38.17	2320.34	65.51	2.09	0.63	3.17	0.95
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.7	Clutch 3	87.20	7.73	0.14	27	2.3	33	2	2473.19	15.48	2316.09	28.12	994.69	19.58	2157.51	15.48	131.51	22.76	3.23	1.05	2.13	0.75	8	27.75	10.21	1016.58	369.67	1019.76	370.82	2163.59	92.88	129.00	38.17	2320.34	65.51	2.09	0.63	3.17	0.95
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.7	Clutch 4	92.50	7.73	0.14	27	2.3	33	2	2473.19	15.48	2316.09	28.12	994.69	19.58	2157.51	15.48	131.51	22.76	3.23	1.05	2.13	0.75	8	27.75	10.21	1016.58	369.67	1019.76	370.82	2163.59	92.88	129.00	38.17	2320.34	65.51	2.09	0.63	3.17	0.95
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.7	Clutch 5	92.10	7.73	0.14	27	2.3	33	2	2473.19	15.48	2316.09	28.12	994.69	19.58	2157.51	15.48	131.51	22.76	3.23	1.05	2.13	0.75	8	27.75	10.21	1016.58	369.67	1019.76	370.82	2163.59	92.88	129.00	38.17	2320.34	65.51	2.09	0.63	3.17	0.95
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.7	Clutch 6	94.60	7.73	0.14	27	2.3	33	2	2473.19	15.48	2316.09	28.12	994.69	19.58	2157.51	15.48	131.51	22.76	3.23	1.05	2.13	0.75	8	27.75	10.21	1016.58	369.67	1019.76	370.82	2163.59	92.88	129.00	38.17	2320.34	65.51	2.09	0.63	3.17	0.95
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.7	Clutch 7	93.40	7.73	0.14	27	2.3	33	2	2473.19	15.48	2316.09	28.12	994.69	19.58	2157.51	15.48	131.51	22.76	3.23	1.05	2.13	0.75	8	27.75	10.21	1016.58	369.67	1019.76	370.82	2163.59	92.88	129.00	38.17	2320.34	65.51	2.09	0.63	3.17	0.95
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.7	Clutch 8	94.50	7.73	0.14	27	2.3	33	2	2473.19	15.48	2316.09	28.12	994.69	19.58	2157.51	15.48	131.51	22.76	3.23	1.05	2.13	0.75	8	27.75	10.21	1016.58	369.67	1019.76	370.82	2163.59	92.88	129.00	38.17	2320.34	65.51	2.09	0.63	3.17	0.95
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.7	Clutch 9	95.70	7.73	0.14	27	2.3	33	2	2473.19	15.48	2316.09	28.12	994.69	19.58	2157.51	15.48	131.51	22.76	3.23	1.05	2.13	0.75	8	27.75	10.21	1016.58	369.67	1019.76	370.82	2163.59	92.88	129.00	38.17	2320.34	65.51	2.09	0.63	3.17	0.95
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.3	Clutch 1	90.10	7.35	0.11	27	2.5	33	3	2466.25	18.98	2453.63	19.86	2570.90	10.57	2324.60	18.64	59.07	16.95	1.45	0.72	0.95	0.05	8	71.61	19.69	2623.44	705.56	2631.65	707.76	2327.56	41.16	57.85	15.33	2457.03	45.23	0.94	0.25	1.42	0.38
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.3	Clutch 2	88.20	7.35	0.11	27	2.5	33	3	2466.25	18.98	2453.63	19.86	2570.90	10.57	2324.60	18.64	59.07	16.95	1.45	0.72	0.95	0.05	8	71.61	19.69	2623.44	705.56	2631.65	707.76	2327.56	41.16	57.85	15.33	2457.03	45.23	0.94	0.25	1.42	0.38
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.3	Clutch 3	86.50	7.35	0.11	27	2.5	33	3	2466.25	18.98	2453.63	19.86	2570.90	10.57	2324.60	18.64	59.07	16.95	1.45	0.72	0.95	0.05	8	71.61	19.69	2623.44	705.56	2631.65	707.76	2327.56	41.16	57.85	15.33	2457.03	45.23	0.94	0.25	1.42	0.38
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.3	Clutch 4	88.12	7.35	0.11	27	2.5	33	3	2466.25	18.98	2453.63	19.86	2570.90	10.57	2324.60	18.64	59.07	16.95	1.45	0.72	0.95	0.05	8	71.61	19.69	2623.44	705.56	2631.65	707.76	2327.56	41.16	57.85	15.33	2457.03	45.23	0.94	0.25	1.42	0.38
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.3	Clutch 5	89.56	7.35	0.11	27	2.5	33	3	2466.25	18.98	2453.63	19.86	2570.90	10.57	2324.60	18.64	59.07	16.95	1.45	0.72	0.95	0.05	8	71.61	19.69	2623.44	705.56	2631.65	707.76	2327.56	41.16	57.85	15.33	2457.03	45.23	0.94	0.25	1.42	0.38
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.3	Clutch 6	90.10	7.35	0.11	27	2.5	33	3	2466.25	18.98	2453.63	19.86	2570.90	10.57	2324.60	18.64	59.07	16.95	1.45	0.72	0.95	0.05	8	71.61	19.69	2623.44	705.56	2631.65	707.76	2327.56	41.16	57.85	15.33	2457.03	45.23	0.94	0.25	1.42	0.38
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.3	Clutch 7	87.40	7.35	0.11	27	2.5	33	3	2466.25	18.98	2453.63	19.86	2570.90	10.57	2324.60	18.64	59.07	16.95	1.45	0.72	0.95	0.05	8	71.61	19.69	2623.44	705.56	2631.65	707.76	2327.56	41.16	57.85	15.33	2457.03	45.23	0.94	0.25	1.42	0.38
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.3	Clutch 8	90.10	7.35	0.11	27	2.5	33	3	2466.25	18.98	2453.63	19.86	2570.90	10.57	2324.60	18.64	59.07	16.95	1.45	0.72	0.95	0.05	8	71.61	19.69	2623.44	705.56	2631.65	707.76	2327.56	41.16	57.85	15.33	2457.03	45.23	0.94	0.25	1.42	0.38
laboratory	Amphiprion sebae (fish)	212772	marinespecies.org	pH 7.3	Clutch 9	87.80	7.35	0.11	27	2.5	33	3	2466.25	18.98	2453.63	19.86	2570.90	10.57	2324.60	18.64	59.07	16.95	1.45	0.72	0.95	0.05	8	71.61	19.69	2623.44	705.56	2631.65	707.76	2327.56	41.16	57.85	15.33	2457.03	45.23	0.94	0.25	1.42	0.38