Haider, Fouzia; Timm, Stefan; Bruhns, Torben; Noor, Mizra Nusrat; Sokolova, Inna M (2020): RNA to DNA ratio in Mya arenaria under prolonged food limitation [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.922164, In: Haider, F et al. (2020): Effect of food limitation on energy metabolism and burrowing activity of an infaunal marine bivalve, Mya arenaria [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.922165
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Published: 2020-09-01 • DOI registered: 2020-10-07
Supplement to:
Haider, Fouzia; Timm, Stefan; Bruhns, Torben; Noor, Mizra Nusrat; Sokolova, Inna M (2020): Effects of prolonged food limitation on energy metabolism and burrowing activity of an infaunal marine bivalve, Mya arenaria. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 250, 110780, https://doi.org/10.1016/j.cbpa.2020.110780
Comment:
RNA/DNA ratio was measured using fluorometry at 525 nm excitation and 600 nm emission (SprectraMax M2 microplate reader).
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Treatment: salinity | T:sal | Haider, Fouzia | |||
| 2 | Treatment: temperature | T:temp | °C | Haider, Fouzia | ||
| 3 | Treatment: light condition | T:light | Haider, Fouzia | |||
| 4 | Treatment: food | T:food | Haider, Fouzia | |||
| 5 | Sample ID | Sample ID | Haider, Fouzia | |||
| 6 | Tissue, mass | Tis mass | g | Haider, Fouzia | ||
| 7 | Volume | Vol | ml | Haider, Fouzia | Buffer | |
| 8 | Dilution factor | Dilution | Haider, Fouzia | |||
| 9 | Deoxyribonucleic acid per volume | DNA | µg/ml | Haider, Fouzia | SpectraMax microplate reader (Molecular Devices) | |
| 10 | Ribonucleic acid per volume | RNA | µg/ml | Haider, Fouzia | SpectraMax microplate reader (Molecular Devices) | |
| 11 | RNA/DNA ratio | RNA/DNA | Haider, Fouzia | SpectraMax microplate reader (Molecular Devices) |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
209 data points
Data
| 1 T:sal | 2 T:temp [°C] | 3 T:light | 4 T:food | 5 Sample ID | 6 Tis mass [g] | 7 Vol [ml] | 8 Dilution | 9 DNA [µg/ml] | 10 RNA [µg/ml] | 11 RNA/DNA |
|---|---|---|---|---|---|---|---|---|---|---|
| 15 | 15 | Dark | Fed | FC1 | 0.0192 | 0.192 | 0.625 | 58.223 | 333.145 | 5.72 |
| 15 | 15 | Dark | Fed | FC2 | 0.0217 | 0.217 | 0.625 | 47.609 | 485.065 | 10.19 |
| 15 | 15 | Dark | Fed | FC3 | 0.0217 | 0.217 | 0.625 | 54.798 | 569.441 | 10.39 |
| 15 | 15 | Dark | Fed | FC4 | 0.0194 | 0.194 | 0.625 | 55.292 | 493.163 | 8.92 |
| 15 | 15 | Dark | 22 days food limited | S2C1 | 0.0266 | 0.266 | 0.625 | 49.044 | 481.221 | 9.81 |
| 15 | 15 | Dark | 22 days food limited | S2C2 | 0.0224 | 0.224 | 0.625 | 58.861 | 369.090 | 6.27 |
| 15 | 15 | Dark | 22 days food limited | S2C3 | 0.0240 | 0.240 | 0.625 | 80.875 | 395.078 | 4.89 |
| 15 | 15 | Dark | 22 days food limited | S2C4 | 0.0208 | 0.208 | 0.625 | 41.317 | 363.327 | 8.79 |
| 15 | 15 | Dark | 22 days food limited | S2C5 | 0.0290 | 0.290 | 0.625 | 40.839 | 374.879 | 9.18 |
| 15 | 15 | Dark | 33 days food limited | S3C1 | 0.0243 | 0.243 | 0.625 | 50.580 | 419.839 | 8.30 |
| 15 | 15 | Dark | 33 days food limited | S3C2 | 0.0321 | 0.321 | 0.625 | 140.217 | 584.509 | 4.17 |
| 15 | 15 | Dark | 33 days food limited | S3C3 | 0.0222 | 0.222 | 0.625 | 76.650 | 586.622 | 7.65 |
| 15 | 15 | Dark | 33 days food limited | S3C4 | 0.0200 | 0.200 | 0.625 | 62.379 | 467.611 | 7.50 |
| 15 | 15 | Dark | 33 days food limited | S3C5 | 0.0200 | 0.200 | 0.625 | 45.926 | 327.593 | 7.13 |
| 15 | 15 | Dark | 42 days food limited | S4C1 | 0.0244 | 0.244 | 0.625 | 46.449 | 346.898 | 7.47 |
| 15 | 15 | Dark | 42 days food limited | S4C2 | 0.0237 | 0.237 | 0.625 | 82.297 | 189.535 | 2.30 |
| 15 | 15 | Dark | 42 days food limited | S4C3 | 0.0182 | 0.182 | 0.625 | 41.248 | 391.958 | 9.50 |
| 15 | 15 | Dark | 42 days food limited | S4C4 | 0.0255 | 0.255 | 0.625 | 66.686 | 285.394 | 4.28 |
| 15 | 15 | Dark | 42 days food limited | S4C5 | 0.0234 | 0.234 | 0.625 | 37.000 | 671.657 | 18.15 |
