Keller, Isabel Salome; Roth, Olivia (2020): Effects of paternal investment on sexual immune dimorphism in three species of sex-role reversed pipefish: candidate gene expression (Fluidigm) of females and males in different gravity stages [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.921055
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Abstract:
We sampled the three pipefish species, Nerophis ophidion, Syngnathus rostellatus and Syngnathus typhle in April and May 2016 by snorkelling in seagrass meadows around the Kiel Fjord (54°44'N; 9°53'E). All animals were kept for acclimatisation to laboratory conditions for two weeks in the aquaria at GEOMAR. Males were randomly assigned to one of the three reproductive stage: non-pregnant males, pregnant males and males after parturition. Only males assigned to be sampled at pregnant and after parturition stage were permitted to mate with the females. At mid pregnancy, males were either left in the tanks to be sampled after parturition or sampled immediately. Fish were killed by an overdose of MS222 and their weight and length were measured. Gills were collected and stored in RNAlater (Qiagen) for candidate gene expression analysis.
RNA was extracted from gill tissue with RNeasy 96 Universal Tissue Kit (Qiagen) following the manufacturers protocol for animal tissues. RNA yield was measured by spectrometry (NanoDrop ND-1000; peQLab) and 200 ng/µl were used for reverse transcription with QuantiTect®Reverse-Transcription Kit (Qiagen). We used primer pairs for immune system related and metabolism related genes (Beemelmanns and Roth 2016 doi:10.1002/ece3.2391). The expression patterns of genes were measured using a Fluidigm-BioMarkTM system based on 96.96 dynamic arrays (GE-Chip).
Keyword(s):
Supplement to:
Keller, Isabel Salome; Roth, Olivia (2020): Parental investment and immune dynamics in sex-role reversed pipefishes. PLoS ONE, 15(9), e0228974, https://doi.org/10.1371/journal.pone.0228974
Comment:
Negative delta threshold cycle, quantitative polymerase chain reaction (-dCt values)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Identification | ID | Keller, Isabel Salome | |||
| 2 | Species | Species | Keller, Isabel Salome | |||
| 3 | Gravity stage | Gravity stg | Keller, Isabel Salome | |||
| 4 | Sex | Sex | Keller, Isabel Salome | |||
| 5 | Length, total | TL | mm | Keller, Isabel Salome | ||
| 6 | Mass | Mass | g | Keller, Isabel Salome | ||
| 7 | Condition factor | CF | g/cm3 | Keller, Isabel Salome | ||
| 8 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | aif: Allograft inflammation factor | |
| 9 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | c1qsco: Recognition sub-component (C1q) | |
| 10 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | c3: Complement component 3 | |
| 11 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | c9: Complement component 9 | |
| 12 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | calrucul: Calreticulin | |
| 13 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | cd45: CD45 (leukocyte common antigen) | |
| 14 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | cd74: MHC II antigen presentation | |
| 15 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | cf: Coagulation factor II | |
| 16 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | ddplgy: UDP-glycosyltransferase | |
| 17 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | fcrl5: Fc receptor like 5 | |
| 18 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | g6pd: Glucose 6-phosphate dehydrogenase (G6PD) | |
| 19 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | h2k1: Histocompatibility II, K1 | |
| 20 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | hsp60: Heat shock protein 60 | |
| 21 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | igmic: Immunoglobulin M light chain | |
| 22 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | ikcytok: Ik cytokine (RED-production) | |
| 23 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | il10: Interleukin 10 | |
| 24 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | il8: Interleukin 8 | |
| 25 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | intf: Interferon induced transmembrane protein 3 | |
| 26 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | kin: Kinesin | |
| 27 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | lectpt1: Lectin protein type I | |
| 28 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | lectpt2: Lectin protein type II | |
| 29 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | lpstnf: LSP - induced TNF _ factor (LITAF) | |
| 30 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | lympcyt: lymphocyte cytosolic protein 2 | |
| 31 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | lymph75: Lymphocyt antigen 75 | |
| 32 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | nramp: Natural resistance-assoc. macrophage protein | |
| 33 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | pepdgly: Peptidoglycan recognition protein II | |
| 34 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | ripop: Ribosomal protein | |
| 35 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | stat1: Signal transducer and activator of transcription 1 | |
| 36 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | tap: Tag-binding protein (Tapasin) | |
| 37 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | tnf: Tumor necrosis factor | |
| 38 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | tranfe: Transferrin | |
| 39 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | tspo: Translocator protein | |
| 40 | Minus Δ threshold cycle, quantitative polymerase chain reaction | Minus Δ Ct qPCR | Keller, Isabel Salome | High Throughput PCR, Fluidigm BioMark HD | tyroprot: Tyroproteinkinase |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
3299 data points
Data
| 1 ID | 2 Species | 3 Gravity stg | 4 Sex | 5 TL [mm] | 6 Mass [g] | 7 CF [g/cm3] | 8 Minus Δ Ct qPCR (aif: Allograft inflammation f...) | 9 Minus Δ Ct qPCR (c1qsco: Recognition sub-compo...) | 10 Minus Δ Ct qPCR (c3: Complement component 3) | 11 Minus Δ Ct qPCR (c9: Complement component 9) | 12 Minus Δ Ct qPCR (calrucul: Calreticulin) | 13 Minus Δ Ct qPCR (cd45: CD45 (leukocyte common ...) | 14 Minus Δ Ct qPCR (cd74: MHC II antigen presenta...) | 15 Minus Δ Ct qPCR (cf: Coagulation factor II) | 16 Minus Δ Ct qPCR (ddplgy: UDP-glycosyltransferase) | 17 Minus Δ Ct qPCR (fcrl5: Fc receptor like 5) | 18 Minus Δ Ct qPCR (g6pd: Glucose 6-phosphate deh...) | 19 Minus Δ Ct qPCR (h2k1: Histocompatibility II, K1) | 20 Minus Δ Ct qPCR (hsp60: Heat shock protein 60) | 21 Minus Δ Ct qPCR (igmic: Immunoglobulin M light...) | 22 Minus Δ Ct qPCR (ikcytok: Ik cytokine (RED-pro...) | 23 Minus Δ Ct qPCR (il10: Interleukin 10) | 24 Minus Δ Ct qPCR (il8: Interleukin 8) | 25 Minus Δ Ct qPCR (intf: Interferon induced tran...) | 26 Minus Δ Ct qPCR (kin: Kinesin) | 27 Minus Δ Ct qPCR (lectpt1: Lectin protein type I) | 28 Minus Δ Ct qPCR (lectpt2: Lectin protein type II) | 29 Minus Δ Ct qPCR (lpstnf: LSP - induced TNF _ f...) | 30 Minus Δ Ct qPCR (lympcyt: lymphocyte cytosolic...) | 31 Minus Δ Ct qPCR (lymph75: Lymphocyt antigen 75) | 32 Minus Δ Ct qPCR (nramp: Natural resistance-ass...) | 33 Minus Δ Ct qPCR (pepdgly: Peptidoglycan recogn...) | 34 Minus Δ Ct qPCR (ripop: Ribosomal protein) | 35 Minus Δ Ct qPCR (stat1: Signal transducer and ...) | 36 Minus Δ Ct qPCR (tap: Tag-binding protein (Tap...) | 37 Minus Δ Ct qPCR (tnf: Tumor necrosis factor) | 38 Minus Δ Ct qPCR (tranfe: Transferrin) | 39 Minus Δ Ct qPCR (tspo: Translocator protein) | 40 Minus Δ Ct qPCR (tyroprot: Tyroproteinkinase) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 129 | Nerophis ophidion | female | 281 | 1.142 | 0.00005 | 4.26615 | 5.51558 | -5.01207 | -3.47981 | 3.47167 | 3.46214 | -0.24238 | -9.38057 | 0.01402 | 1.07779 | 5.90175 | 4.01276 | -0.97071 | 4.22865 | 4.02750 | -2.87939 | 0.43647 | 2.27133 | -2.69212 | 5.86134 | 6.17666 | 4.44426 | 4.54535 | -0.24782 | 1.16953 | -3.35369 | 5.98149 | -0.73618 | 6.09583 | -4.63328 | -2.45515 | 4.29147 | -0.27673 | |
| 130 | Nerophis ophidion | female | 248 | 1.021 | 0.00007 | 4.50662 | 5.38509 | -8.27591 | -3.59560 | 2.59767 | 2.38667 | 1.15989 | -5.92028 | 0.21163 | 1.06058 | 7.38621 | 5.07476 | -0.82759 | 4.39994 | 4.60854 | -2.18749 | 3.43104 | 2.53932 | -4.32657 | 10.12743 | 7.72685 | 6.88535 | 4.90070 | 0.02304 | 2.01021 | -3.27892 | 7.37204 | 1.99778 | 5.76127 | -2.81428 | -2.48755 | 5.02017 | -0.59761 | |
| 149 | Nerophis ophidion | female | 197 | 0.497 | 0.00007 | 1.62660 | -2.73360 | -5.45245 | 0.03976 | 2.28870 | 2.45842 | 2.81574 | -5.26116 | 0.99601 | 2.39475 | -2.15416 | 3.28122 | -5.48245 | -4.90249 | -2.34185 | -5.56459 | 2.28905 | -2.46361 | -5.01544 | -0.24177 | -3.06723 | -2.42960 | -1.65450 | 2.95810 | -4.91068 | -3.77291 | 0.31953 | -0.81332 | -3.58679 | 0.68646 | 1.19806 | 4.26027 | 1.69026 | |
| 151 | Nerophis ophidion | female | 166 | 0.323 | 0.00007 | -7.43512 | -4.85959 | -3.89142 | -2.54052 | 3.29827 | 1.76753 | -6.86454 | -6.39280 | -7.44818 | -7.49155 | -6.73699 | -6.27999 | -8.43920 | 2.70456 | -6.19757 | -1.70885 | 0.95458 | -6.67585 | -7.98642 | -2.12320 | -7.71843 | -11.65679 | -6.88327 | -7.92538 | 2.71082 | -2.12165 | -1.79003 | -6.46847 | -5.72558 | -2.07248 | -1.76120 | -8.50805 | -1.10312 | |
| 161 | Nerophis ophidion | female | 187 | 0.537 | 0.00008 | 6.97645 | 6.20308 | -3.71962 | -3.27124 | 5.41418 | 1.06492 | -1.15339 | -6.46573 | 1.45205 | 0.21419 | 8.13057 | 4.99323 | -0.10540 | 1.27897 | 5.42425 | -3.18572 | 0.46431 | 5.43888 | -1.55686 | 8.68061 | 7.08757 | 4.74022 | 5.26414 | 0.58646 | 2.04018 | -2.47584 | 7.54378 | 0.43503 | 7.20454 | -2.73948 | -2.14943 | 5.60935 | -1.03222 | |
| 170 | Nerophis ophidion | female | 250 | 1.067 | 0.00007 | 5.46384 | 6.42266 | -5.96991 | -2.78296 | 5.23689 | 1.56763 | -0.95413 | -5.80341 | 1.37373 | 0.68111 | 8.11103 | 5.23399 | -0.37723 | 3.19758 | 5.85124 | -2.76691 | 0.99256 | 2.97557 | -1.93933 | 3.19605 | 6.12838 | 5.56255 | 5.31371 | 0.21686 | 3.63778 | -2.76156 | 7.88729 | 0.91117 | 7.43783 | -1.28040 | -4.28822 | 5.86379 | -1.20773 | |
| 83 | Syngnathus rostellatus | female | 155 | 1.258 | 0.00034 | -3.19797 | 2.62341 | -2.57491 | -5.38901 | -4.12025 | -3.48175 | -1.42898 | -6.09639 | -1.98720 | -4.05276 | 1.36071 | -0.75094 | -2.42924 | -0.14050 | -0.47986 | -5.81019 | 2.22698 | 0.65491 | -4.36202 | 1.72323 | 2.04417 | 1.12860 | 4.10311 | -1.69465 | -0.06959 | -0.96369 | 2.75836 | -4.11827 | 2.02632 | -7.55023 | -0.71209 | -0.24465 | -3.08917 | |
| 96 | Syngnathus rostellatus | female | 153 | 1.112 | 0.00031 | -6.21901 | 4.49022 | -9.44774 | -7.08539 | -5.02669 | 1.77273 | 1.36338 | -10.31769 | -0.49558 | 0.85524 | 5.54116 | 1.57423 | -2.18687 | -3.32502 | -1.64626 | -3.97223 | 0.26300 | 0.60867 | -4.36299 | 2.40571 | 2.78418 | 5.89468 | 3.13976 | -3.51178 | 0.48168 | -2.77563 | 7.12172 | -7.05059 | 3.11877 | -8.03430 | -2.49147 | 1.50486 | -7.55136 | |
| 97 | Syngnathus rostellatus | female | 126 | 0.587 | 0.00029 | 4.21143 | 4.82911 | 0.33987 | -2.10249 | 3.84021 | -1.13503 | -1.92481 | -8.05693 | -0.41261 | -1.19545 | 5.93709 | 2.15441 | -0.69372 | 3.30794 | 4.02410 | -2.83890 | -4.88845 | 1.73437 | -2.13478 | 3.78098 | 1.93003 | 3.72351 | 3.65547 | 0.34522 | 1.43370 | -1.26711 | 4.89096 | -2.03853 | 5.65691 | -3.54315 | -0.47459 | 3.66183 | -2.21514 | |
| 100 | Syngnathus rostellatus | female | 122 | 0.295 | 0.00016 | 1.23171 | 5.39721 | -8.79589 | -5.41158 | -1.03827 | 0.93847 | -1.35662 | -10.58875 | -2.38821 | -1.06411 | 4.53478 | -0.65950 | -1.12046 | -2.13429 | 3.09964 | -2.63180 | 1.29546 | 1.82714 | -3.30211 | 7.69359 | 4.23616 | 3.02603 | 3.51136 | -0.90661 | 0.08741 | -3.74657 | 4.77969 | -1.60278 | 4.65912 | -3.39625 | -5.67848 | 2.25171 | -2.71497 | |
| 101 | Syngnathus rostellatus | female | 131 | 0.603 | 0.00027 | 0.30468 | 4.26277 | -4.73711 | -4.93323 | -3.09389 | 0.37660 | -0.49211 | -8.82119 | -3.03043 | -1.15748 | 4.61253 | 0.03682 | -1.68641 | -1.54143 | 2.86903 | -2.53058 | -1.78324 | 1.95503 | -3.50411 | 1.62577 | 3.94527 | 5.72230 | 3.35030 | -0.86325 | 0.28243 | -1.66460 | 4.96939 | -1.65031 | 3.84774 | -6.09314 | -3.41595 | 3.02499 | -3.90603 | |
| 102 | Syngnathus rostellatus | female | 128 | 0.555 | 0.00026 | 5.97228 | 7.07019 | -1.52898 | -3.24570 | 4.56110 | 2.45655 | 0.19605 | -6.83648 | 0.40235 | 1.74520 | 6.70125 | 3.84434 | -0.31456 | 3.63282 | 5.80016 | -0.47320 | 3.22582 | 3.68081 | -2.21233 | 12.51193 | 8.31248 | 6.21936 | 6.08844 | -0.06228 | 3.58921 | -0.50330 | 6.87576 | 0.06038 | 8.11579 | -3.75791 | -2.45270 | 4.07605 | -0.75677 | |
| 123 | Syngnathus rostellatus | female | 127 | 0.487 | 0.00024 | 4.94625 | 6.64571 | -5.26376 | -2.82316 | 5.89376 | 3.00891 | -1.12564 | -6.58283 | 1.66160 | 0.83202 | 7.97041 | 4.51380 | 0.23410 | 4.50141 | 5.11855 | -1.96623 | -0.29089 | 3.11027 | -1.72486 | 8.21609 | 5.46505 | 5.69077 | 5.30326 | 0.26991 | 2.27212 | -3.42203 | 6.55182 | 0.45341 | 7.30154 | -4.49717 | -4.08626 | 5.47140 | -1.14593 | |
| 131 | Syngnathus rostellatus | female | 138 | 0.695 | 0.00026 | 1.21932 | 5.47226 | -6.82758 | -4.58328 | 0.94586 | 1.32879 | 0.22099 | -8.30367 | -1.03567 | -0.03257 | 6.44330 | 3.17680 | -0.25438 | -1.63819 | 3.70921 | -2.09391 | 1.04842 | 1.24961 | -3.95253 | 3.26112 | 4.06438 | 6.89171 | 3.52004 | -0.38006 | -1.22175 | -2.73051 | 6.41257 | 0.25438 | 0.51472 | -3.16866 | -2.03082 | 4.61616 | -3.20518 | |
| 68 | Syngnathus typhle | female | 136 | 0.808 | 0.00032 | -2.83747 | 2.46799 | -2.34220 | -2.45338 | -0.43373 | -1.12949 | 0.89512 | -4.30049 | -1.19942 | -1.58894 | 3.52836 | 1.63234 | -3.93678 | -0.61651 | 1.28879 | 0.84018 | -2.07007 | -1.63672 | -0.86522 | 4.26159 | 3.72647 | 3.06186 | 2.01763 | -0.08550 | -2.00763 | -2.00882 | 4.11420 | -1.02726 | 2.30808 | -4.65047 | -1.57591 | 1.96194 | -4.00025 | |
| 88 | Syngnathus typhle | female | 143 | 0.743 | 0.00025 | 0.50382 | 3.11319 | -3.63562 | -3.41349 | 1.16592 | -3.70557 | -0.01602 | -5.59391 | -3.76050 | -4.84383 | -0.35620 | -1.65826 | -0.68948 | 1.07543 | -0.66157 | -3.79120 | -2.05138 | 1.45624 | -2.72474 | 1.63091 | 1.11870 | 3.24478 | 1.57582 | -0.49885 | -2.06868 | -0.50549 | 1.62945 | -2.79558 | 1.56083 | -3.24320 | -2.55146 | -1.72948 | -3.54646 | |
| 89 | Syngnathus typhle | female | 128 | 0.508 | 0.00024 | -1.74569 | -1.36104 | -5.72782 | -2.63356 | -3.65390 | 0.53443 | 0.07859 | -8.17936 | 0.09767 | -0.53552 | -1.61786 | 1.31640 | 0.88907 | -0.33711 | 2.45938 | -1.13047 | 1.87857 | 0.52732 | -9.88470 | -0.20368 | 5.89688 | 4.77006 | 2.26794 | 0.28754 | -0.50362 | -3.30765 | -0.60365 | -2.87709 | -2.40236 | -5.61701 | -2.37599 | 3.32239 | -2.70889 | |
| 90 | Syngnathus typhle | female | 127 | 0.511 | 0.00025 | 1.90561 | -2.19070 | -8.36728 | -4.24536 | -0.85196 | 0.34056 | -0.61379 | -9.42011 | -1.43028 | 0.71224 | -2.44752 | 2.12930 | -0.47454 | 0.00602 | 3.03578 | -5.20827 | 0.44002 | 2.34727 | -3.35058 | 4.02912 | 5.23474 | -2.80187 | 4.12182 | 0.36486 | -1.40011 | -4.39740 | 5.09481 | -3.14475 | 4.15708 | 0.35793 | -4.04465 | 2.22367 | -1.02631 | |
| 93 | Syngnathus typhle | female | 133 | 0.842 | 0.00036 | -1.64961 | -0.63429 | -0.57881 | -3.12418 | 4.22274 | -2.77390 | 4.74978 | -2.53710 | -2.30810 | 4.32879 | -0.79276 | 5.21526 | 3.00929 | -2.91721 | -0.11513 | 2.60357 | -3.98536 | 0.75352 | -3.24697 | 3.64492 | 0.38222 | -0.90463 | -0.47962 | 4.89214 | -2.13556 | -2.18568 | 3.79409 | -4.03877 | -2.04886 | -3.57855 | -2.39536 | 6.19431 | 3.62430 | |
| 94 | Syngnathus typhle | female | 168 | 1.567 | 0.00033 | 2.93313 | -3.16156 | -1.20631 | 1.34629 | -3.13074 | -3.30177 | -1.50717 | -7.13954 | -4.01444 | 3.70128 | -1.02754 | -2.43935 | -3.26836 | 3.50473 | -2.11037 | 1.97607 | 3.59558 | -1.38897 | -2.71667 | 5.86838 | 0.27552 | -1.51661 | -1.20038 | 4.26463 | -3.57072 | 1.96261 | 6.60157 | -3.93714 | -2.18663 | 1.99299 | 2.50459 | -1.18257 | 2.99679 | |
| 120 | Syngnathus typhle | female | 162 | 1.605 | 0.00038 | -4.81615 | 0.63134 | -5.62795 | -5.62822 | -5.45604 | -2.85125 | -0.41283 | -7.58624 | -3.60867 | -4.70842 | 0.78897 | -1.33593 | -2.12390 | -3.03751 | -0.80216 | -3.09326 | -2.73632 | -2.26253 | -9.42016 | 2.26715 | 0.35737 | 0.85240 | 0.82749 | -2.89587 | -1.60198 | -2.39948 | 2.29737 | -3.92843 | 0.73957 | -4.90038 | 0.28860 | -1.89039 | -3.85709 | |
| 214 | Nerophis ophidion | after parturition | male | 156 | 0.282 | 0.00007 | 4.06097 | 4.93831 | -5.40757 | -1.97079 | 3.99487 | 1.23302 | -3.90035 | -6.19390 | 1.85639 | 1.43560 | 7.18342 | 4.83897 | -0.64770 | 3.59391 | 4.90430 | -2.12636 | 1.04595 | 2.75966 | -1.70405 | 8.81039 | 5.50522 | 6.53332 | 4.75389 | -0.00992 | 4.07964 | -3.04121 | 7.36427 | 1.54985 | 6.48669 | -4.39876 | -3.94028 | 5.47982 | -1.23367 |
| 219 | Nerophis ophidion | after parturition | male | 160 | 0.315 | 0.00008 | 5.28330 | 6.42482 | -5.21113 | -2.51206 | 4.83487 | 1.13469 | -0.65662 | -6.62343 | 1.37604 | 1.11855 | 7.55283 | 6.59364 | -0.24493 | 2.49746 | 5.32303 | -1.95082 | -1.33730 | 3.88978 | -2.33528 | 7.78179 | 4.91956 | -0.42632 | 4.82662 | -0.61510 | 3.66812 | -3.83844 | 7.39389 | 0.78954 | 6.38569 | -2.95427 | -1.79200 | 4.85106 | -1.74689 |
| 220 | Nerophis ophidion | after parturition | male | 170 | 0.376 | 0.00008 | 5.76479 | 5.67470 | -1.94896 | -2.32250 | 4.00135 | 0.75466 | -0.22431 | -8.12579 | -0.41938 | -0.71193 | 6.81121 | 3.11541 | -1.03365 | 3.21341 | 4.60848 | -2.70321 | 1.67524 | 3.62390 | -3.12165 | 4.51800 | 2.87876 | 5.82637 | 5.27621 | -0.11425 | 1.64092 | -2.45068 | 6.23335 | -0.48765 | 6.93116 | -3.51841 | -2.72703 | 4.67540 | -0.33559 |
| 221 | Nerophis ophidion | after parturition | male | 152 | 0.223 | 0.00006 | 2.74201 | 4.85814 | -4.58440 | -2.95329 | 3.76726 | 2.51509 | -0.48890 | -6.55796 | 0.96607 | -2.20089 | 7.66550 | 2.91416 | -1.05172 | 3.00058 | 4.22192 | -4.17542 | 0.06649 | 2.43846 | -2.06242 | 4.69118 | 3.98558 | 5.74238 | 4.14182 | 0.02726 | 3.14018 | -3.92131 | 6.34878 | -0.08329 | 6.12364 | -3.89664 | -4.53334 | 5.15129 | -1.37866 |
| 227 | Nerophis ophidion | after parturition | male | 150 | 0.282 | 0.00008 | 3.76452 | 4.17448 | -7.79274 | -2.70659 | 4.77988 | 2.59741 | -5.61170 | -7.47312 | 1.16551 | 0.73996 | 6.90643 | 4.19842 | -0.71450 | 2.01917 | 3.80467 | -3.78277 | 0.10409 | 2.14780 | -2.26496 | 7.66778 | 3.41554 | 3.48186 | 3.79612 | 0.00182 | 2.86274 | -3.83610 | 5.93190 | 0.55506 | 5.77298 | -6.05712 | -4.65813 | 4.91218 | -1.63131 |
| 140 | Nerophis ophidion | non-pregnant | male | 143 | 0.277 | 0.00009 | 3.37822 | 3.94905 | -3.46818 | -2.79563 | 4.07020 | -0.48562 | -2.74996 | -8.91272 | 0.19336 | 0.87120 | 6.63157 | 2.59687 | -0.97388 | 2.78424 | 4.03385 | -3.25366 | 0.41540 | 1.18814 | -1.34258 | 6.62944 | 4.41160 | 5.51361 | 4.33305 | 0.04417 | 2.47481 | -4.27002 | 5.40316 | -1.52804 | 6.17059 | -5.64053 | -0.58502 | 4.39153 | -2.02017 |
| 141 | Nerophis ophidion | non-pregnant | male | 145 | 0.214 | 0.00007 | 7.53081 | -1.23271 | -0.20158 | 1.14932 | -4.39218 | 5.45737 | 4.08469 | -5.62950 | -5.21272 | 4.33698 | -1.81946 | 6.76522 | -4.57184 | 6.39440 | -1.26911 | 1.98099 | -3.22331 | -7.99772 | 1.83635 | 3.30905 | 1.54396 | 1.36687 | -2.89107 | 4.01134 | 6.40066 | 1.56819 | 2.12449 | -1.42322 | 0.10982 | 1.61736 | 1.92865 | -4.20623 | 2.58672 |
| 198 | Nerophis ophidion | non-pregnant | male | 119 | 0.155 | 0.00009 | 7.04416 | 8.40985 | -0.68824 | 0.66266 | 6.50146 | 4.97071 | 3.59803 | -3.18962 | -3.35819 | -5.50899 | 9.11997 | 6.27857 | -3.21283 | 5.90774 | -4.86601 | -6.22587 | -6.14770 | 6.41796 | 1.34970 | -4.64593 | -5.19840 | -3.96836 | 7.51724 | 3.52468 | 5.91401 | 1.08153 | 1.94954 | -1.38911 | -4.48973 | 1.13070 | 1.44199 | 6.69937 | 2.10006 |
| 199 | Nerophis ophidion | non-pregnant | male | 140 | 0.238 | 0.00009 | 6.80246 | 8.16816 | -0.92994 | 0.42096 | 6.25976 | 4.72901 | 3.35633 | -7.02730 | -4.93377 | 3.60863 | 8.87827 | 6.03687 | -5.50895 | 5.66604 | 6.92812 | 1.25263 | -6.93245 | 6.17627 | -7.40255 | -4.98825 | 6.37581 | 6.70586 | 7.27554 | 3.28298 | 5.67231 | 0.83983 | 9.14147 | -2.70593 | 8.11269 | 0.88900 | 1.20029 | 6.45767 | 1.85836 |
| 200 | Nerophis ophidion | non-pregnant | male | 119 | 0.151 | 0.00009 | -5.14914 | 8.00196 | -1.09613 | 0.25477 | 6.09357 | 4.56282 | 3.19014 | -3.59750 | -9.44792 | 3.44244 | 8.71208 | -6.63388 | -7.48884 | 5.49985 | -7.43241 | 1.08644 | 3.74987 | 6.01007 | 0.94181 | -2.52011 | -8.56840 | -7.04209 | 7.10935 | 3.11679 | -3.95561 | 0.67364 | 1.11502 | -5.28313 | -5.41527 | 0.72281 | 1.03410 | 6.29148 | 1.69217 |
| 201 | Nerophis ophidion | non-pregnant | male | 154 | 0.282 | 0.00008 | -4.23957 | 7.88411 | -6.90548 | 0.13691 | 5.97571 | 4.44496 | 3.07229 | -3.71536 | -3.01328 | 3.32458 | -5.46192 | -6.00125 | -2.95836 | 5.38199 | -7.74586 | 0.96858 | 3.63202 | 5.89222 | -4.85891 | -2.20840 | -8.04614 | -5.81831 | 6.99149 | 2.99893 | -1.92846 | 0.55578 | 1.79436 | -3.11937 | -4.86431 | 0.60496 | 0.91624 | -6.38928 | 1.57431 |
| 209 | Nerophis ophidion | non-pregnant | male | 175 | 0.448 | 0.00008 | -7.54230 | -5.34662 | -3.45417 | -0.90157 | 1.34738 | 1.51710 | -6.01822 | -5.41246 | -6.01106 | -6.51576 | -6.27773 | -3.95644 | -4.92566 | 1.25688 | -5.89193 | -0.27179 | 1.34773 | 1.80467 | -6.11693 | -0.66602 | -4.95323 | 3.02907 | 3.12805 | 2.01678 | -6.76268 | -0.28524 | 0.87358 | -6.65608 | -5.56576 | -6.75742 | 0.25673 | 3.31895 | 0.74894 |
| 210 | Nerophis ophidion | non-pregnant | male | 162 | 0.304 | 0.00007 | 2.72418 | -4.60304 | -4.82867 | -4.93030 | 3.38629 | -5.62479 | -3.76434 | -4.86645 | -1.21632 | 3.49234 | 5.42233 | 4.37881 | 2.17284 | -6.88186 | 4.08828 | 1.76713 | -3.35482 | -4.62305 | -3.38909 | 6.83651 | 3.03155 | 5.06799 | -5.46801 | 4.05569 | 1.09007 | 1.75367 | -4.86176 | -2.08795 | 4.63783 | 1.78404 | 2.29565 | 5.35786 | 2.78785 |
| 137 | Nerophis ophidion | pregnant | male | 146 | 0.339 | 0.00011 | 6.34139 | 7.04542 | -0.34206 | -0.32092 | 6.25591 | 2.12629 | -3.97710 | -6.49663 | 2.01667 | 1.67344 | 8.48968 | 4.27437 | 0.16251 | 4.78503 | 6.16141 | -2.80345 | 4.27738 | 4.36548 | -1.27408 | 10.32287 | 6.45174 | 1.68581 | 6.52860 | 0.63471 | 3.68208 | 0.60047 | 7.95635 | 0.73562 | 8.18295 | -3.30934 | 1.04740 | 6.76924 | -0.57295 |
| 138 | Nerophis ophidion | pregnant | male | 152 | 0.356 | 0.00010 | 4.58861 | 5.88519 | -4.56557 | -1.65193 | 5.23831 | 1.35794 | -1.28351 | -6.74422 | 1.67922 | 0.49411 | 8.33084 | 5.31898 | 0.15137 | 4.33637 | 5.40425 | -1.86026 | 1.85825 | 2.40842 | -1.22277 | 7.95965 | 5.29625 | 5.55398 | 5.29601 | 0.79207 | 3.89889 | -2.39757 | 7.59667 | 0.35834 | 7.11115 | -3.38950 | -3.24856 | 6.61121 | -1.57449 |
| 165 | Nerophis ophidion | pregnant | male | 166 | 0.363 | 0.00008 | 5.17907 | 4.85702 | -4.59538 | -3.04906 | 4.56210 | -0.46860 | -4.33085 | -4.65272 | 3.40582 | -0.97451 | 7.67621 | 4.96292 | 0.33574 | 1.66803 | 4.43050 | -3.52482 | 0.21310 | 2.44157 | -1.22864 | 2.97323 | 3.38098 | -0.87691 | 3.80065 | 1.01008 | 3.91711 | -3.07523 | 7.00917 | 1.07656 | 5.48560 | -2.72224 | -2.99304 | 0.06543 | -2.26359 |
| 166 | Nerophis ophidion | pregnant | male | 161 | 0.408 | 0.00010 | 5.57411 | 6.25448 | -0.87222 | -1.52577 | 4.97704 | 1.80842 | -1.12862 | -6.37975 | 6.84041 | 0.66799 | 8.46384 | 5.45416 | -0.71920 | 1.66667 | 5.51727 | -2.23913 | 0.85396 | 4.42094 | -1.47274 | 6.90856 | 5.92215 | 5.73883 | 5.23513 | 0.40943 | 4.20001 | -1.83678 | 7.14363 | 0.93109 | 7.21478 | -2.73222 | -1.90622 | 5.92999 | -1.18794 |
| 168 | Nerophis ophidion | pregnant | male | 142 | 0.322 | 0.00011 | 6.89026 | 6.22023 | -3.07142 | -3.08742 | 3.99731 | 0.79682 | 4.42463 | -5.82255 | 1.50019 | 0.59453 | 6.08894 | 3.75827 | 0.36759 | 3.82002 | 5.08154 | -4.40064 | -0.37519 | 5.95105 | -1.28000 | 12.72603 | 5.56742 | 5.78226 | 5.05245 | 0.12415 | 2.18417 | -3.03531 | 7.78843 | -0.00089 | 6.19546 | -0.12137 | -3.81067 | 4.78992 | -2.33478 |
| 192 | Nerophis ophidion | pregnant | male | 155 | 0.536 | 0.00014 | -0.02530 | 1.66132 | 3.20882 | 4.55972 | -0.84986 | -1.13257 | -0.04012 | 0.70744 | 7.10013 | -2.33802 | 2.66817 | 3.00077 | 6.51565 | -0.47431 | 1.36698 | 5.39139 | 8.05482 | 1.72515 | 5.24676 | 5.61181 | 0.48791 | -0.24028 | 1.71070 | 7.42174 | 9.81107 | -0.48853 | 6.97310 | 1.90784 | 2.41382 | 5.02776 | 5.33905 | 1.31331 | 5.99712 |
| 205 | Nerophis ophidion | pregnant | male | 191 | 0.633 | 0.00009 | -7.92313 | 3.12616 | -3.96824 | -1.41564 | 0.83330 | 1.00302 | 1.36034 | -9.73003 | -0.45939 | 0.93935 | 2.86934 | 1.82582 | -6.90495 | 0.74281 | -6.65558 | -0.78586 | 0.83365 | 1.29059 | -8.39979 | -4.65632 | -5.59181 | -4.58492 | -4.53365 | 1.50270 | -7.07803 | -0.79932 | -1.13454 | -4.50885 | -5.18828 | -0.76894 | -0.25734 | 2.80487 | 0.23487 |
| 8 | Syngnathus rostellatus | after parturition | male | 136 | 0.645 | 0.00026 | 4.80792 | 4.71387 | -6.90057 | -5.01782 | 1.49743 | 1.84237 | -1.24188 | -6.75134 | -0.97864 | 0.10098 | 4.77426 | 2.19986 | -0.55515 | 3.45768 | 4.53578 | -3.17241 | 1.18284 | 2.20992 | -3.87919 | 8.37049 | 4.10810 | 6.25722 | 5.04062 | -0.19052 | 0.16741 | -2.58627 | 4.81188 | -1.80740 | 6.67412 | -4.63434 | -0.83672 | 1.37390 | -2.62760 |
| 10 | Syngnathus rostellatus | after parturition | male | 135 | 0.747 | 0.00030 | 3.84097 | 5.20667 | -3.89142 | -2.54052 | 3.29827 | 1.76753 | 0.39485 | -6.39280 | -0.00012 | 0.64714 | 5.91678 | 3.07538 | -0.58459 | 2.70456 | 3.96663 | -1.70885 | 0.95458 | 3.21478 | -1.85349 | 6.65230 | 3.41433 | 3.74438 | 4.31405 | 0.32150 | 2.71082 | -2.12165 | 6.17999 | -0.14007 | 5.15120 | -2.07248 | -1.76120 | 3.49619 | -1.10312 |
| 17 | Syngnathus rostellatus | after parturition | male | 120 | 0.472 | 0.00027 | 5.67701 | 6.16741 | -3.48472 | -3.59610 | 3.81258 | 2.56892 | -1.57585 | -6.24412 | 1.07054 | 0.74612 | 6.68698 | 5.05469 | 0.30036 | 6.41076 | 5.01188 | -1.09837 | 0.02838 | 4.39972 | -1.91026 | 10.94436 | 6.73129 | -0.09250 | 4.19805 | 0.59459 | 2.53504 | -2.97720 | 6.76442 | 1.93312 | 6.66049 | -4.04008 | -2.42592 | 4.20030 | -1.39719 |
| 63 | Syngnathus rostellatus | after parturition | male | 102 | 0.373 | 0.00035 | -0.71629 | 0.46952 | -1.82764 | -1.36402 | -0.98055 | 3.14363 | 1.02283 | -3.78593 | 1.68121 | 3.07996 | 5.00995 | -0.56491 | 1.76045 | -0.50662 | 0.62118 | -2.70026 | -1.79117 | -1.09872 | -3.40329 | 1.18491 | 1.29779 | 2.59269 | 2.41735 | 0.18715 | -0.70554 | 0.00581 | 0.56640 | -0.12812 | 1.44131 | -2.27400 | -2.57174 | -1.43438 | -1.62431 |
| 64 | Syngnathus rostellatus | after parturition | male | 120 | 0.939 | 0.00054 | -1.29552 | 2.59200 | -7.82943 | -4.40304 | -1.30673 | 0.45900 | 0.23471 | -7.36118 | -0.43424 | -0.83519 | 4.67847 | 3.74117 | -0.13733 | -0.39252 | 1.72749 | -2.72626 | 0.24220 | 0.18271 | -2.38452 | 3.76043 | 4.36641 | 4.11157 | 1.42788 | -0.09377 | -0.83004 | -6.51286 | 6.10434 | -0.52652 | -2.22723 | -4.02391 | -3.57904 | 2.73559 | -2.16744 |
| 81 | Syngnathus rostellatus | after parturition | male | 140 | 0.721 | 0.00026 | -1.63697 | 5.01071 | -5.26978 | -6.27303 | 2.96891 | 1.34691 | 0.04524 | -7.77116 | -1.69709 | 0.14445 | 6.30573 | 3.08637 | -0.64701 | -1.60278 | 3.23619 | -2.62399 | -2.13200 | 0.86830 | -4.16470 | 5.03128 | 5.35187 | 6.50377 | 2.92995 | -0.80060 | -0.72028 | -4.12439 | 6.08382 | -1.19360 | 4.70890 | -5.40965 | -3.93786 | 3.38169 | -2.46329 |
| 107 | Syngnathus rostellatus | after parturition | male | 135 | 0.616 | 0.00025 | 4.09925 | 7.87948 | -5.78347 | -3.63437 | 3.99721 | 3.28362 | 2.72232 | -5.84608 | 0.55369 | 2.48584 | 7.12695 | 4.48908 | 1.31432 | 0.45268 | 5.31144 | -0.42482 | 2.36800 | 4.67051 | -3.35968 | 11.04881 | 6.41443 | 9.10651 | 5.23790 | -0.04319 | 1.75719 | -1.30409 | 8.38841 | 0.06754 | 6.46063 | -3.04896 | -1.76552 | 4.11955 | -4.91250 |
| 122 | Syngnathus rostellatus | after parturition | male | 153 | 1.073 | 0.00030 | 1.86353 | 5.35577 | -6.57413 | -3.94950 | 0.98914 | 2.77404 | 0.59459 | -7.32460 | -0.90604 | 1.29060 | 6.97393 | 3.58101 | -0.55259 | -0.29057 | 4.08409 | -1.88931 | -0.96309 | 2.24662 | -2.95064 | 2.06609 | 4.80674 | 5.98942 | 4.82610 | -0.71399 | 0.06791 | -3.57211 | 6.86632 | -1.34256 | 1.99532 | -3.07338 | -3.31145 | 4.05540 | -3.89177 |
| 66 | Syngnathus rostellatus | non-pregnant | male | 150 | 0.706 | 0.00021 | -0.00761 | 3.32278 | -7.88907 | -4.72352 | -1.52717 | 1.40209 | 0.36130 | -8.70364 | -1.03506 | -0.26640 | 4.85162 | 3.24001 | -0.66387 | -0.89793 | 2.57969 | -2.29377 | -0.38309 | 0.01219 | -2.52085 | 6.07457 | 4.97493 | 3.98818 | 2.24317 | 0.14045 | -0.54050 | -5.39339 | -1.85190 | -0.62657 | 3.22899 | -5.32333 | -0.42452 | 2.98351 | -2.96149 |
| 67 | Syngnathus rostellatus | non-pregnant | male | 120 | 0.406 | 0.00023 | 1.22037 | 3.43485 | -7.16578 | -4.10971 | -0.62006 | 0.55865 | 0.13675 | -9.38334 | -1.54202 | 0.57407 | 5.40845 | 3.16102 | -0.94522 | 1.23071 | 2.61775 | -2.18528 | 0.39976 | 2.50628 | -2.73628 | 1.08482 | 3.52174 | 3.95700 | 2.69058 | -0.08483 | -1.03104 | -4.90469 | 6.27810 | -8.82224 | 4.38433 | -4.30859 | 2.01798 | 4.06277 | -1.45926 |
| 78 | Syngnathus rostellatus | non-pregnant | male | 127 | 0.627 | 0.00031 | 1.06710 | 4.99821 | -7.15781 | -4.97879 | 1.01611 | 0.50217 | -1.78952 | -9.37894 | -2.11373 | -0.26471 | 6.07464 | 2.18848 | -0.48029 | -0.27031 | 3.27428 | -2.02595 | 0.12584 | 1.84588 | -2.91246 | 6.21878 | 4.93697 | 6.23960 | 2.95865 | -0.20982 | -1.13506 | -4.04742 | 5.26928 | -2.52456 | 6.05860 | -4.66112 | -3.64789 | 3.76663 | -2.37963 |
| 79 | Syngnathus rostellatus | non-pregnant | male | 126 | 0.607 | 0.00030 | 2.05938 | 5.46150 | -6.09631 | -4.53130 | -0.98380 | 1.17932 | -1.28552 | -8.99646 | -1.47311 | 0.13525 | 5.26453 | 0.73981 | 0.35851 | 1.54812 | 3.35973 | -2.17720 | -0.05757 | 3.90921 | -4.19825 | -4.92307 | 5.68175 | 7.00698 | 3.45280 | 0.05018 | -0.68294 | -3.39936 | 5.45484 | 1.38499 | 5.31256 | -2.74751 | -3.38525 | 2.59747 | -1.95444 |
| 80 | Syngnathus rostellatus | non-pregnant | male | 130 | 0.514 | 0.00023 | 4.32616 | 5.49769 | -6.65725 | -2.96963 | 1.94731 | 1.48859 | 0.84210 | -9.31241 | -1.17436 | 0.06940 | 5.74353 | 1.40517 | 0.79385 | -2.34492 | 3.62211 | -0.56400 | 0.76718 | 4.48424 | -3.38611 | 6.75570 | 5.05402 | 5.30231 | 3.67504 | -0.81104 | 0.55236 | -3.99575 | 6.04729 | -2.49137 | 5.18012 | -3.34832 | -4.75681 | 2.98073 | -2.49206 |
| 119 | Syngnathus rostellatus | non-pregnant | male | 113 | 0.319 | 0.00022 | 2.01825 | 6.54410 | -7.67172 | -3.90709 | 0.20610 | 2.31783 | 0.29384 | -7.65403 | -0.64748 | 0.65300 | 6.06960 | 2.59748 | 0.50570 | 1.61905 | 3.69649 | -1.42963 | 0.22199 | 2.17292 | -2.34151 | 10.97395 | 7.13011 | 6.78430 | 4.33080 | 0.17098 | 0.88525 | -1.97105 | 6.70390 | 0.17157 | 5.62772 | -3.31580 | -2.55671 | 3.66338 | -2.52371 |
| 180 | Syngnathus rostellatus | non-pregnant | male | 129 | 0.559 | 0.00026 | 4.05894 | 4.83213 | -3.20594 | -2.88432 | 4.82080 | 3.84306 | -1.98652 | -6.80810 | 1.73171 | 0.27254 | 8.19036 | 5.49514 | -0.64109 | 4.20335 | 4.44732 | -2.61042 | 0.09684 | 1.86763 | -1.66597 | 4.13881 | 5.25305 | 3.65099 | 3.56747 | 0.59240 | 3.39630 | -2.27568 | 7.40592 | 1.21323 | 6.39538 | -2.06688 | -3.35788 | 5.78434 | -1.55849 |
| 182 | Syngnathus rostellatus | non-pregnant | male | 142 | 0.757 | 0.00026 | 4.50779 | 5.83942 | -6.74832 | -2.63544 | 3.72131 | 0.40536 | -1.05845 | -6.92403 | 0.01281 | -0.68377 | 7.83738 | 3.08648 | -0.51542 | 1.74672 | 5.09359 | -5.52400 | 0.95212 | 3.88844 | -2.17896 | -3.01204 | 3.25037 | 4.26593 | 5.28745 | -0.71092 | 2.64647 | -3.94960 | 6.42726 | -1.27000 | 6.69361 | -4.30846 | -3.03910 | 4.96868 | -1.44957 |
| 41 | Syngnathus rostellatus | pregnant | male | 133 | 0.941 | 0.00040 | 6.25345 | 5.54535 | -4.46877 | -2.81708 | 2.84841 | 0.32642 | -4.37650 | -8.32241 | 0.06828 | 1.10689 | 5.67274 | 2.38422 | 0.01165 | 4.18986 | 4.39525 | -0.02148 | -0.70848 | 4.17256 | -1.84840 | 9.31399 | 4.59452 | 3.40032 | 4.35805 | 0.00309 | 1.46246 | -4.96305 | 5.50449 | -2.70994 | 5.80047 | -3.33809 | 0.95688 | 2.93735 | -2.11035 |
| 45 | Syngnathus rostellatus | pregnant | male | 130 | 0.743 | 0.00034 | 5.09014 | 5.43297 | -6.99326 | -2.64376 | 3.65105 | -0.69049 | -5.02046 | -9.69562 | -0.19842 | -0.38019 | 5.89582 | 2.63899 | -0.19138 | 4.81852 | 4.21883 | -2.91174 | -0.95503 | 1.36097 | -2.32953 | 6.16861 | 3.93615 | 4.16048 | 3.97163 | 0.01071 | 1.37374 | -4.56085 | 5.34330 | -3.33995 | 6.16080 | -5.90049 | -5.26174 | 3.89262 | -1.96646 |
| 60 | Syngnathus rostellatus | pregnant | male | 155 | 0.959 | 0.00026 | 2.80401 | 5.27164 | -8.68206 | -4.76668 | 3.56563 | -0.81078 | -1.77597 | -11.01958 | 0.10559 | -0.94004 | 4.92031 | 1.53646 | -0.57826 | 4.56538 | 3.97597 | -2.43075 | -0.39998 | 0.83553 | -2.03304 | 6.16789 | 4.99226 | 6.15888 | 3.62600 | 0.20368 | 0.99808 | -4.48430 | 4.87734 | -1.79552 | 6.06938 | -5.10445 | -3.45635 | 2.72914 | -1.69911 |
| 61 | Syngnathus rostellatus | pregnant | male | 135 | 0.837 | 0.00034 | 4.43830 | 5.06219 | -4.57407 | -3.64583 | 3.86668 | -0.00389 | -2.47358 | -7.92845 | 0.32828 | 1.09072 | 6.24730 | 2.96127 | -0.23212 | 4.73034 | 4.39160 | -3.20939 | 1.77017 | 3.84433 | -1.42899 | 6.98085 | 5.34837 | 3.97234 | 4.44154 | 0.37295 | -0.26112 | -3.86432 | 5.31596 | -1.91467 | 6.34159 | -4.79057 | -2.27875 | 4.20192 | -0.97208 |
| 62 | Syngnathus rostellatus | pregnant | male | 130 | 0.872 | 0.00040 | 5.00105 | 4.32756 | -4.04230 | -3.37254 | 3.23896 | 0.71444 | -2.79413 | -6.08556 | 0.15291 | 0.15066 | 6.83963 | 5.65529 | -1.07464 | 4.48024 | 4.34941 | -2.03208 | 3.17676 | 2.79143 | -1.57286 | 1.64445 | 1.82647 | 4.33698 | 4.84350 | 0.28108 | 2.75249 | -4.00457 | 6.62795 | 1.09790 | 6.15421 | -5.48842 | -3.24416 | 5.16838 | -1.41715 |
| 71 | Syngnathus rostellatus | pregnant | male | 112 | 0.458 | 0.00033 | 4.22579 | 4.54730 | -5.21333 | -3.90571 | 4.10711 | 0.83021 | -1.56720 | -6.75213 | 0.89799 | 0.53675 | 7.29809 | 4.71480 | -0.68926 | 5.64306 | 4.40339 | -2.27557 | 0.48004 | 1.27826 | -1.93249 | 3.38337 | 2.84122 | 4.79847 | 4.38845 | 0.05235 | 3.12264 | -3.75431 | 6.72199 | -1.09618 | 6.67303 | -4.93910 | -4.67474 | 4.83573 | -1.21683 |
| 136 | Syngnathus rostellatus | pregnant | male | 156 | 1.049 | 0.00028 | 5.54947 | 5.76162 | -4.66522 | -2.15519 | 5.38173 | 1.56588 | 1.20372 | -6.93619 | 2.13769 | 1.64771 | 7.56076 | 4.25005 | -0.47178 | 3.37812 | 5.49954 | -3.14710 | 2.25009 | 3.62828 | -2.46010 | 4.87473 | 3.78336 | 5.08604 | 5.80203 | -0.10476 | 2.32567 | -2.52817 | 7.07385 | 0.19856 | 7.56784 | -0.61989 | -3.00053 | 5.15253 | -1.25902 |
| 24 | Syngnathus typhle | after parturition | male | 139 | 1.115 | 0.00042 | -4.17060 | 1.69681 | -5.65632 | -10.81118 | -6.07308 | -3.18760 | -0.24225 | -7.61461 | -3.04795 | -3.54517 | 1.51126 | -2.26379 | -2.83648 | -2.90916 | -0.34146 | -6.68492 | -2.19788 | -3.44587 | -4.84738 | 1.37328 | 0.89655 | 2.97205 | 1.57778 | -2.93606 | -5.09097 | -5.49571 | 2.30512 | -7.58882 | 1.33921 | -4.47546 | 0.62819 | -1.47449 | -5.31262 |
| 36 | Syngnathus typhle | after parturition | male | 143 | 0.895 | 0.00031 | -0.43189 | 4.11442 | -8.40403 | -3.77666 | 0.08498 | -4.37986 | -4.11296 | -12.32329 | -2.59556 | -0.85133 | 4.97937 | 0.00829 | -1.01176 | 4.59057 | 2.56833 | -2.18941 | -0.36367 | 0.97588 | -4.24562 | -0.92660 | 2.55253 | 3.89271 | 3.39599 | -0.95601 | -2.71881 | -6.52723 | 5.10320 | -5.29759 | 0.01666 | -4.00099 | -4.10494 | 1.96971 | -3.55185 |
| 46 | Syngnathus typhle | after parturition | male | 134 | 0.898 | 0.00037 | 1.33587 | 4.72795 | -6.13484 | -6.73249 | -1.76977 | -0.92202 | -1.84072 | -10.59443 | -2.12133 | -1.05349 | 4.64635 | 0.67228 | -0.53415 | 1.31097 | 3.20954 | -4.38355 | 1.85878 | 4.34008 | -4.43621 | 5.55528 | -4.15719 | 6.32780 | 2.94259 | -0.48928 | -2.14942 | -2.98287 | 4.57679 | -2.40879 | -0.85557 | -4.16798 | -3.76785 | 2.74675 | -3.39302 |
| 50 | Syngnathus typhle | after parturition | male | 144 | 1.012 | 0.00034 | -2.27691 | 5.72517 | -8.68290 | -6.79657 | -5.79562 | -1.46979 | -1.66153 | -11.68315 | -2.21576 | -1.46201 | 3.98102 | 0.20280 | -0.31840 | 0.63017 | 1.16423 | -4.36906 | 0.78278 | 4.56457 | -3.81393 | 7.03173 | 3.20498 | 6.53826 | 4.44232 | -0.02957 | -1.74636 | -2.87949 | 4.92346 | -6.57266 | -1.15391 | -4.59629 | -3.77606 | 1.90846 | -3.88820 |
| 91 | Syngnathus typhle | after parturition | male | 144 | 1.298 | 0.00043 | 3.90228 | 5.11030 | -6.84361 | -1.82142 | -2.44668 | -0.67900 | -1.70793 | -8.37899 | -2.50753 | 0.13026 | 5.74087 | -0.30053 | -1.29316 | 0.17998 | 3.95938 | -2.86966 | 2.40928 | 4.85117 | -4.35591 | 7.79597 | 6.07514 | 5.95635 | 4.89732 | -0.66101 | -1.95324 | -4.20944 | 5.62002 | -2.35769 | 4.54996 | -0.66989 | -4.35011 | 2.23949 | -3.52058 |
| 153 | Syngnathus typhle | after parturition | male | 149 | 1.191 | 0.00036 | -6.67021 | 2.76618 | -7.30764 | -1.77563 | 0.47331 | -9.54018 | 1.00035 | -7.16524 | -0.81938 | -6.62879 | -6.16481 | -4.65741 | -7.94504 | 0.38282 | -4.91021 | -1.14585 | 0.47366 | -5.60432 | -7.14692 | -1.58794 | -6.80606 | -5.74219 | -5.48617 | 1.14272 | -6.53429 | -1.15931 | 3.36499 | -6.57994 | 1.72485 | -6.93232 | -0.61733 | 2.44489 | -0.12512 |
| 188 | Syngnathus typhle | after parturition | male | 149 | 1.612 | 0.00049 | 0.94203 | 4.50109 | -7.95133 | -2.63537 | -0.42015 | 1.09482 | -0.32353 | -8.78906 | -0.89102 | -0.41776 | 5.11864 | 2.32616 | 0.03300 | 0.62182 | 2.91599 | -1.17228 | 1.69554 | 2.13099 | -4.08611 | 0.55930 | 3.67123 | 5.60326 | 3.32262 | 0.06552 | -1.25194 | -3.94774 | 6.57941 | -7.63094 | 4.49817 | -2.21598 | -3.52340 | 2.68066 | -2.53227 |
| 189 | Syngnathus typhle | after parturition | male | 155 | 1.118 | 0.00030 | 0.93230 | 5.58135 | -8.00643 | -4.29531 | -1.55673 | -1.13776 | -2.51617 | -10.95904 | -2.15098 | -0.87662 | 4.17936 | -0.04625 | -0.11113 | -1.72198 | 3.47213 | -2.94833 | 0.72152 | 4.34263 | -8.51698 | 7.54750 | 5.55013 | 6.40442 | 4.54509 | -0.07983 | -2.34435 | -4.23644 | 4.26676 | -3.29689 | 0.41017 | -4.84912 | -4.34317 | 1.53957 | -2.56155 |
| 29 | Syngnathus typhle | non-pregnant | male | 111 | 0.438 | 0.00032 | 3.20779 | 4.83033 | -6.82628 | -3.05829 | -1.13121 | 0.31003 | 0.31667 | -8.14707 | -1.48437 | -0.33663 | 5.51797 | 3.34858 | 0.09207 | -0.13208 | 3.20405 | -2.10724 | -0.05751 | 5.21554 | -2.18770 | 0.12975 | 4.27259 | 6.14358 | 2.79654 | 0.86588 | -0.76869 | -4.68309 | 6.81541 | -7.71749 | 5.51411 | -0.78022 | -3.23284 | 3.23449 | -4.02473 |
| 30 | Syngnathus typhle | non-pregnant | male | 101 | 0.283 | 0.00027 | 1.36500 | 4.10196 | -6.92509 | -4.84528 | -1.09565 | 1.69347 | -0.43110 | -9.42400 | -1.32824 | 1.02816 | 4.99896 | 1.21851 | 0.06692 | 3.19884 | 2.46012 | -0.17855 | 0.11214 | 1.06804 | -3.12347 | 1.06346 | 5.32134 | 4.19663 | 3.01535 | 0.22946 | -1.37477 | -4.84599 | 5.78514 | -8.02601 | 4.99299 | -4.97353 | -3.59456 | 2.77217 | -1.51909 |
| 37 | Syngnathus typhle | non-pregnant | male | 107 | 0.393 | 0.00032 | 1.49967 | 4.09638 | -6.01604 | -4.53067 | -1.38660 | 0.63785 | -1.85891 | -9.99366 | -1.97965 | -0.73319 | 5.02720 | 1.20890 | -0.97458 | 2.53577 | 3.21262 | -3.04486 | 1.95433 | 3.41475 | -2.79579 | 5.32340 | 5.48022 | 4.98442 | 3.22801 | -0.39414 | -2.45444 | -3.36128 | 5.51307 | -2.19680 | 4.49322 | -5.03384 | 0.69462 | 3.44030 | -2.55546 |
| 215 | Syngnathus typhle | non-pregnant | male | 172 | 1.725 | 0.00034 | 0.35830 | 3.31327 | -5.85456 | -1.22854 | 1.02040 | 1.19013 | -5.42820 | -6.39338 | -4.07190 | 1.12646 | 3.05645 | 2.01293 | -5.01671 | -4.68018 | -5.98344 | -5.49482 | 1.02076 | -2.58154 | -6.55357 | -4.54350 | -8.63556 | 2.70210 | -3.90708 | -5.27615 | -1.27582 | -0.61221 | -1.93843 | -5.59061 | -5.08985 | -0.58184 | -0.07024 | 2.99198 | 0.42197 |
| 218 | Syngnathus typhle | non-pregnant | male | 152 | 1.202 | 0.00034 | 3.21107 | 0.32354 | -0.92836 | 1.62423 | 3.87318 | 4.04290 | 4.40022 | -3.83072 | -3.02786 | 3.97923 | 5.90922 | 4.86570 | -3.90152 | -5.67114 | -3.48881 | 2.25402 | -3.03271 | -5.87457 | 0.54862 | -1.86266 | -4.55827 | -1.76316 | 5.65386 | 4.54258 | -4.12542 | 2.24056 | 1.95408 | 0.77115 | 5.12472 | 2.27093 | 2.78253 | -1.96529 | 3.27474 |
| 222 | Syngnathus typhle | non-pregnant | male | 149 | 1.375 | 0.00042 | 0.17625 | 3.13122 | -3.96319 | -1.41059 | 0.83835 | 1.00808 | -5.45692 | -5.92148 | -0.45434 | 0.94441 | -5.80699 | 1.83088 | -5.16334 | 0.74786 | 1.54035 | -7.62553 | 0.83871 | -4.00229 | -6.81347 | -2.76779 | -8.44164 | -6.51368 | 2.61903 | 1.50776 | -4.80718 | -0.79427 | 0.76168 | -6.02614 | 2.08989 | -6.16699 | -0.25229 | 2.80993 | 0.23992 |
| 9 | Syngnathus typhle | pregnant | male | 130 | 1.262 | 0.00057 | -5.29926 | -0.01560 | -6.05512 | -4.79250 | -5.28756 | -2.36940 | -0.36327 | -7.58510 | -3.60690 | -3.75637 | 2.14042 | -1.30427 | -1.38160 | -2.02006 | -0.96900 | -4.68343 | -2.45495 | -3.41585 | -3.78818 | 0.56033 | -0.87125 | 0.24026 | 1.26816 | -3.44564 | -0.85419 | -1.98424 | 2.05152 | -4.35560 | -4.37039 | -4.42788 | -2.03200 | -0.92597 | -3.46367 |
| 133 | Syngnathus typhle | pregnant | male | 140 | 1.300 | 0.00047 | 0.32407 | 3.27904 | -3.81537 | -1.26277 | 0.98617 | 1.15590 | 1.51322 | -5.77366 | -0.30652 | 1.09223 | 3.02222 | 1.97870 | -0.22727 | -9.68959 | -5.38700 | -0.63299 | 0.98653 | -6.16756 | -2.33839 | -4.44074 | -8.06620 | -4.89842 | -5.46219 | 1.65558 | -7.23229 | -5.87101 | -3.76484 | -2.11585 | 2.23771 | -0.61607 | -7.75555 | 2.95775 | 0.38774 |
| 134 | Syngnathus typhle | pregnant | male | 134 | 1.119 | 0.00047 | -5.49377 | 2.53470 | -5.17734 | -2.00711 | 0.24184 | -4.91656 | -6.67274 | -10.12856 | -7.58371 | 0.34789 | 2.27788 | 1.23436 | -6.13181 | -6.05424 | 0.94383 | -1.37732 | -5.98474 | 0.69913 | -6.96971 | -0.70242 | -4.65148 | -3.96720 | -4.71560 | 0.91124 | -7.46671 | -1.39078 | 1.93294 | -10.07107 | -5.31901 | -1.36041 | -0.84881 | 2.21341 | -0.35660 |
| 154 | Syngnathus typhle | pregnant | male | 144 | 1.403 | 0.00047 | -7.33882 | -2.22430 | -3.48977 | -0.93717 | 1.31178 | -4.21792 | -2.90525 | -5.44806 | 0.01909 | -6.20898 | -3.91484 | -2.47143 | -4.46022 | 1.22128 | -4.04566 | -0.30738 | -7.55428 | 1.76907 | -9.36723 | 3.79304 | -5.37012 | -3.48784 | 3.09246 | 1.98118 | -4.57885 | -4.49349 | 2.00180 | -1.79025 | -3.51647 | -0.29047 | 0.22113 | -2.39817 | 0.71334 |
| 159 | Syngnathus typhle | pregnant | male | 165 | 2.246 | 0.00050 | -0.23799 | -5.45334 | -7.24389 | -1.82483 | 0.42411 | -9.40541 | 0.95115 | -8.72045 | -0.86858 | -8.41916 | -9.29066 | -5.20095 | -7.33043 | 0.33361 | -6.92678 | -1.19505 | 0.42446 | -4.30732 | -2.90045 | 0.42529 | -5.95700 | -6.05073 | 2.20479 | -6.91893 | -6.28953 | -1.20851 | 2.42822 | -2.67792 | 1.67565 | -1.17813 | -0.66653 | -4.25656 | -7.32514 |