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Data Publisher for Earth & Environmental Science

Keller, Isabel Salome; Roth, Olivia (2020): Effects of paternal investment on sexual immune dimorphism in three species of sex-role reversed pipefish: candidate gene expression (Fluidigm) of females and males in different gravity stages. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.921055 (dataset in review)

Abstract:
We sampled the three pipefish species, Nerophis ophidion, Syngnathus rostellatus and Syngnathus typhle in April and May 2016 by snorkelling in seagrass meadows around the Kiel Fjord (54°44'N; 9°53'E). All animals were kept for acclimatisation to laboratory conditions for two weeks in the aquaria at GEOMAR. Males were randomly assigned to one of the three reproductive stage: non-pregnant males, pregnant males and males after parturition. Only males assigned to be sampled at pregnant and after parturition stage were permitted to mate with the females. At mid pregnancy, males were either left in the tanks to be sampled after parturition or sampled immediately. Fish were killed by an overdose of MS222 and their weight and length were measured. Gills were collected and stored in RNAlater (Qiagen) for candidate gene expression analysis.
RNA was extracted from gill tissue with RNeasy 96 Universal Tissue Kit (Qiagen) following the manufacturers protocol for animal tissues. RNA yield was measured by spectrometry (NanoDrop ND-1000; peQLab) and 200 ng/µl were used for reverse transcription with QuantiTect®Reverse-Transcription Kit (Qiagen). We used primer pairs for immune system related and metabolism related genes (Beemelmanns and Roth 2016 doi: 10.1002/ece3.2391). The expression patterns of genes were measured using a Fluidigm-BioMarkTM system based on 96.96 dynamic arrays (GE-Chip).
Keyword(s):
Parental investment; pipefish; resource-allocation trade-off; sex-role reversal; sexual immune dimorphism
Related to:
Keller, Isabel Salome; Roth, Olivia (2020): Parental investment and immune dynamics in sex-role reversed pipefishes. PLoS ONE, 15(9), e0228974, https://doi.org/10.1371/journal.pone.0228974
Comment:
Negative delta threshold cycle, quantitative polymerase chain reaction
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1IdentificationIDKeller, Isabel Salome
2SpeciesSpeciesKeller, Isabel Salome
3Gravity stageGravity stgKeller, Isabel Salome
4SexSexKeller, Isabel Salome
5Length, totalTLmmKeller, Isabel Salome
6MassMassgKeller, Isabel Salome
7Condition factorCFg/cm3Keller, Isabel Salome
8Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDaif: Allograft inflammation factor
9Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDc1qsco: Recognition sub-component (C1q)
10Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDc3: Complement component 3
11Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDc9: Complement component 9
12Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDcalrucul: Calreticulin
13Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDcd45: CD45 (leukocyte common antigen)
14Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDcd74: MHC II antigen presentation
15Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDcf: Coagulation factor II
16Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDddplgy: UDP-glycosyltransferase
17Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDfcrl5: Fc receptor like 5
18Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDg6pd: Glucose 6-phosphate dehydrogenase (G6PD)
19Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDh2k1: Histocompatibility II, K1
20Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDhsp60: Heat shock protein 60
21Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDigmic: Immunoglobulin M light chain
22Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDikcytok: Ik cytokine (RED-production)
23Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDil10: Interleukin 10
24Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDil8: Interleukin 8
25Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDintf: Interferon induced transmembrane protein 3
26Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDkin: Kinesin
27Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDlectpt1: Lectin protein type I
28Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDlectpt2: Lectin protein type II
29Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDlpstnf: LSP - induced TNF _ factor (LITAF)
30Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDlympcyt: lymphocyte cytosolic protein 2
31Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDlymph75: Lymphocyt antigen 75
32Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDnramp: Natural resistance-assoc. macrophage protein
33Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDpepdgly: Peptidoglycan recognition protein II
34Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDripop: Ribosomal protein
35Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDstat1: Signal transducer and activator of transcription 1
36Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDtap: Tag-binding protein (Tapasin)
37Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDtnf: Tumor necrosis factor
38Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDtranfe: Transferrin
39Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDtspo: Translocator protein
40Δ threshold cycle, quantitative polymerase chain reactionΔ Ct qPCRKeller, Isabel SalomeHigh Throughput PCR, Fluidigm BioMark HDtyroprot: Tyroproteinkinase
Size:
3299 data points

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