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Käse, Laura; Kraberg, Alexandra Claudia; Metfies, Katja; Neuhaus, Stefan; Sprong, Pim; Fuchs, Bernhard M; Boersma, Maarten; Wiltshire, Karen Helen (2020): Eukaryotic microbial community at the LTER site Helgoland Roads from March 2016 to March 2019. PANGAEA, https://doi.pangaea.de/10.1594/PANGAEA.921026 (DOI registration in progress)

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Abstract:
Water samples were collected from the long-term ecological research (LTER) site at Helgoland and the eukaryotic microbial community was assessed. 18S V4 region was amplified using the primer set 528iF /964iR and amplicon sequencing was performed on an Illumina MiSeq™ sequencer in a 2 × 300 bp paired-end run. Sequence data have been deposited in the European Nucleotide Archive (ENA) at the European Bioinformatics Institute (EMBL-EBI) under accession number PRJEB37135 using the data brokerage service of the German Federation for Biological Data (GFBio). 21 million sequences remained after bioinformatic processing and were clustered into Operational Taxonomic Units (OTUs). The Protist Ribosomal Reference database (PR2), version 4.11.1 was used as reference database.
Keyword(s):
DNA barcoding; eukaryotic plankton; OTU; Phytoplankton; protists; Zooplankton
Related to:
Käse, Laura; Metfies, Katja; Kraberg, Alexandra Claudia; Neuhaus, Stefan; Meunier, Cédric Léo; Wiltshire, Karen Helen; Boersma, Maarten (2021): Metabarcoding analysis suggests that flexible food web interactions in the eukaryotic plankton community are more common than specific predator-prey relationships at Helgoland Roads, North Sea. ICES Journal of Marine Science, fsab058, https://doi.org/10.1093/icesjms/fsab058
Käse, Laura; Metfies, Katja; Neuhaus, Stefan; Boersma, Maarten; Wiltshire, Karen Helen; Kraberg, Alexandra Claudia (2021): Host-parasitoid associations in marine planktonic time series: Can metabarcoding help reveal them? PLoS ONE, 16(1), e0244817, https://doi.org/10.1371/journal.pone.0244817
Further details:
Neuhaus, Stefan; Käse, Laura; Kraberg, Alexandra Claudia; Metfies, Katja; Sprong, Pim; Fuchs, Bernhard M; Boersma, Maarten; Wiltshire, Karen Helen (2020): Eukaryotic microbial community at the LTER site Helgoland Roads. European Nucleotide Archive (ENA), insdc:PRJEB37135
Coverage:
Latitude: 54.188330 * Longitude: 7.900000
Minimum Elevation: -10.0 m * Maximum Elevation: -10.0 m
Event(s):
HelgolandRoads (Kabeltonne) * Latitude: 54.188330 * Longitude: 7.900000 * Elevation: -10.0 m * Location: German Bight, North Sea * Campaign: HelgolandRoadsTimeseries * Basis: Meeresstation Helgoland * Method/Device: Monitoring (MON)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Binary ObjectBinaryKäse, Laura
Size:
2 data points

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