Citation:

PANGAEA.920269

Name: Seawater carbonate chemistry and mass and fine-scale morphological changes of Coccolith Gephyrocapsa oceanica
Published: 2018
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Gephyrocapsa oceanica; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species

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Abstract:

Coccolithophores have been extensively studied to understand the environmental control on calcification in a key biological group influencing the alkalinity of seawater. Previous studies have established that bulk calcification scales with cell division rates under a wide range of pH conditions. Yet, the fine scale ultrastructural changes of the coccoliths and therefore the pH‐sensitive underlying mechanisms altering biomineralization of the coccoliths remain largely under‐constrained. Using circularly polarized light and high resolution microscopy, we have generated mass estimates of cultured Gephyrocapsa oceanica coccoliths grown in medium with pH values ranging from 7.4 to 9.0. These mass estimates representing a bulk calcification response were related to the morphological changes within the coccoliths. From optimal (pH 8.6) down to pH 7.4 conditions, we have observed that impaired cell growth and lower calcite quota are accompanied by a 35% decrease in mean coccolith mass. The data further show that seawater acidification does not homogenously affect calcification of the coccoliths, as a clear reduction in the breadth of the tube (a structure surrounding the central area of the coccoliths) was detected, whereas all other ultrastructural components were far less impacted. We discuss this specific sensitivity to acidification as the consequence of the altered interaction of the acidic polysaccharides used for biomineralization and ambient concentration of protons released by calcification that substantially modify the growth patterns, the morphology and ultimately the mass of the coccoliths.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Gephyrocapsa oceanica; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species

Supplement to:

Hermoso, Michael; Minoletti, Fabrice (2018): Mass and Fine-Scale Morphological Changes Induced by Changing Seawater pH in the Coccolith Gephyrocapsa oceanica. Journal of Geophysical Research: Biogeosciences, 123(9), 2761-2774, https://doi.org/10.1029/2018JG004535

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Hermoso, Michael		study
2	Species	Species		Hermoso, Michael
3	Registration number of species	Reg spec no		Hermoso, Michael
4	Uniform resource locator/link to reference	URL ref		Hermoso, Michael		WoRMS Aphia ID
5	pH	pH		Hermoso, Michael
6	Growth rate	µ	1/day	Hermoso, Michael		Cell
7	Cell, diameter	Cell diam	µm	Hermoso, Michael
8	Gray level	GL		Hermoso, Michael		Coccolith
9	Gray level	GL		Hermoso, Michael		Tube
10	Coccoliths, length	Cocco l	µm	Hermoso, Michael
11	Pore width, coccoliths	Cocco pore width	µm	Hermoso, Michael
12	Coccoliths, area	Cocco area	µm²	Hermoso, Michael
13	Area	Area	µm²	Hermoso, Michael		Tube
14	Area	Area	µm²	Hermoso, Michael		Central area
15	Length	l	µm	Hermoso, Michael		Tube
16	Width	w	µm	Hermoso, Michael		Tube
17	Thickness	Thick	mm	Hermoso, Michael		Tube
18	Breadth	Breadth	µm	Hermoso, Michael		Tube
19	Coccoliths, thickness	Cocco thick	µm	Hermoso, Michael
20	Thickness	Thick	mm	Hermoso, Michael		Shield
21	Coccoliths, volume	Cocco vol	µm³	Hermoso, Michael
22	Surface area	SA	µm²	Hermoso, Michael		Entire coccolith
23	Surface area, standard deviation	SA std dev	±	Hermoso, Michael		Entire coccolith
24	Gray level	GL		Hermoso, Michael		Entire coccolith
25	Gray level, standard deviation	GL std dev	±	Hermoso, Michael		Entire coccolith
26	Length	l	µm	Hermoso, Michael		Entire coccolith
27	Length, standard deviation	l std dev	±	Hermoso, Michael		Entire coccolith
28	Width	w	µm	Hermoso, Michael		Entire coccolith
29	Width, standard deviation	w std dev	±	Hermoso, Michael		Entire coccolith
30	Thickness	Thick	mm	Hermoso, Michael		Entire coccolith
31	Thickness, standard deviation	Thick std dev	±	Hermoso, Michael		Entire coccolith
32	Mass	Mass	pg	Hermoso, Michael		Entire coccolith
33	Mass, standard deviation	Mass std dev	±	Hermoso, Michael		Entire coccolith
34	Surface area	SA	µm²	Hermoso, Michael		Tube (gray level > 100)
35	Surface area, standard deviation	SA std dev	±	Hermoso, Michael		Tube (gray level > 100)
36	Gray level	GL		Hermoso, Michael		Tube (gray level > 100)
37	Gray level, standard deviation	GL std dev	±	Hermoso, Michael		Tube (gray level > 100)
38	Height	h	µm	Hermoso, Michael		Tube (gray level > 100)
39	Height, standard deviation	h std dev	±	Hermoso, Michael		Tube (gray level > 100)
40	Mass	Mass	pg	Hermoso, Michael		Tube (gray level > 100)
41	Mass, standard deviation	Mass std dev	±	Hermoso, Michael		Tube (gray level > 100)
42	Thickness, size normalized	SNT		Hermoso, Michael		Tube (gray level > 100)
43	Thickness, size normalized, standard deviation	SNT std dev	±	Hermoso, Michael		Tube (gray level > 100)
44	Length	l	µm	Hermoso, Michael		Tube (local maxima)
45	Length, standard deviation	l std dev	±	Hermoso, Michael		Tube (local maxima)
46	Width	w	µm	Hermoso, Michael		Tube (local maxima)
47	Width, standard deviation	w std dev	±	Hermoso, Michael		Tube (local maxima)
48	Breadth	Breadth	µm	Hermoso, Michael		Tube (local maxima)
49	Breadth, standard deviation	Breadth std dev	±	Hermoso, Michael		Tube (local maxima)
50	Length	l	µm	Hermoso, Michael		Central area
51	Length, standard deviation	l std dev	±	Hermoso, Michael		Central area
52	Width	w	µm	Hermoso, Michael		Central area
53	Width, standard deviation	w std dev	±	Hermoso, Michael		Central area
54	Surface area	SA	µm²	Hermoso, Michael		Central area
55	Surface area, standard deviation	SA std dev	±	Hermoso, Michael		Central area
56	Surface area	SA	µm²	Hermoso, Michael		Shield
57	Surface area, standard deviation	SA std dev	±	Hermoso, Michael		Shield
58	Mass	Mass	pg	Hermoso, Michael		Shield
59	Mass, standard deviation	Mass std dev	±	Hermoso, Michael		Shield
60	Temperature, water	Temp	°C	Hermoso, Michael
61	Salinity	Sal		Hermoso, Michael
62	Alkalinity, total	AT	µmol/kg	Hermoso, Michael
63	Carbon dioxide	CO2	µmol/kg	Hermoso, Michael
64	Bicarbonate ion	[HCO3]-	µmol/kg	Hermoso, Michael
65	Carbonate ion	[CO3]2-	µmol/kg	Hermoso, Michael
66	Calcite saturation state	Omega Cal		Hermoso, Michael
67	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
68	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
69	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
70	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
71	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
72	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
73	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
74	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
75	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

10895 data points

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