Citation:

PANGAEA.920209

Name: Seawater carbonate chemistry and respiration rates, protein and lipid content of Balanophyllia elegans
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Balanophyllia elegans; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cnidaria; Coast and continental shelf; Laboratory experiment; North Pacific; Respiration; Single species; Temperate

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Abstract:

Ocean acidification (OA), the global decrease in surface water pH from absorption of anthropogenic CO2, may put many marine taxa at risk. However, populations that experience extreme localized conditions, and are adapted to these conditions predicted in the global ocean in 2100, may be more tolerant to future OA. By identifying locally adapted populations, researchers can examine the mechanisms used to cope with decreasing pH. One oceanographic process that influences pH, is wind driven upwelling. Here we compare two Californian populations of the coral Balanophyllia elegans from distinct upwelling regimes, and test their physiological and transcriptomic responses to experimental seawater acidification. We measured respiration rates, protein and lipid content, and gene expression in corals from both populations exposed to pH levels of 7.8 and 7.4 for 29 days. Corals from the population that experiences lower pH due to high upwelling, maintained the same respiration rate throughout the exposure. In contrast, corals from the low upwelling site had reduced respiration rates, protein content, and lipid‐class content at low pH exposure, suggesting they have depleted their energy reserves. Using RNA‐Seq, we found that corals from the high upwelling site upregulated genes involved in calcium ion binding and ion transport, most likely related to pH homeostasis and calcification. In contrast, corals from the low upwelling site downregulated stress response genes at low pH exposure. Divergent population responses to low pH observed in B. elegans highlight the importance of multi‐population studies for predicting a species' response to future OA.

Keyword(s):

Animalia; Balanophyllia elegans; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Cnidaria; Coast and continental shelf; Laboratory experiment; North Pacific; Respiration; Single species; Temperate

Supplement to:

Griffiths, Joanna S; Pan, Tien-Chien Francis; Kelly, Morgan W (2019): Differential responses to ocean acidification between populations of Balanophyllia elegans corals from high and low upwelling environments. Molecular Ecology, https://doi.org/10.1111/mec.15050

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Median Latitude: 36.665875 * Median Longitude: -121.806485 * South-bound Latitude: 34.417750 * West-bound Longitude: -123.711441 * North-bound Latitude: 38.914000 * East-bound Longitude: -119.901530

Date/Time Start: 2016-06-01T00:00:00 * Date/Time End: 2016-06-30T00:00:00

Event(s):

Goleta * Latitude: 34.417750 * Longitude: -119.901530 * Date/Time Start: 2016-06-01T00:00:00 * Date/Time End: 2016-06-30T00:00:00 * Method/Device: Experiment (EXP)

Point_Arena_Cove * Latitude: 38.914000 * Longitude: -123.711441 * Date/Time Start: 2016-06-01T00:00:00 * Date/Time End: 2016-06-30T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Griffiths, Joanna S
2	Type	Type		Griffiths, Joanna S		study
3	Species	Species		Griffiths, Joanna S
4	Registration number of species	Reg spec no		Griffiths, Joanna S
5	Uniform resource locator/link to reference	URL ref		Griffiths, Joanna S		WoRMS Aphia ID
6	Experiment duration	Exp duration	days	Griffiths, Joanna S
7	Treatment	Treat		Griffiths, Joanna S
8	Location	Location		Griffiths, Joanna S
9	Identification	ID		Griffiths, Joanna S		Coral
10	Identification	ID		Griffiths, Joanna S		Jar
11	Globigerinatheka cf. index	G. cf. index		Griffiths, Joanna S
12	Triacylglycerols, per dry mass	TAG/dm	µg/g	Griffiths, Joanna S
13	Fatty acids, total	FA tot	µg/g	Griffiths, Joanna S
14	Sterols	Sterol	µg/g	Griffiths, Joanna S
15	Phospholipid fatty acids	PLFA	µg/g	Griffiths, Joanna S
16	Lipids, total	Lipids tot	µg/g	Griffiths, Joanna S
17	Proteins, total	TPRT	µg/g	Griffiths, Joanna S
18	Dry mass	Dry m	g	Griffiths, Joanna S
19	Dry mass	Dry m	g	Griffiths, Joanna S		soluble
20	Respiration rate, oxygen	Resp O2	µmol/g/h	Griffiths, Joanna S
21	Incubation duration	Inc dur	weeks	Griffiths, Joanna S
22	Temperature, water	Temp	°C	Griffiths, Joanna S
23	Salinity	Sal		Griffiths, Joanna S
24	pH	pH		Griffiths, Joanna S	Potentiometric	total scale
25	Alkalinity, total	AT	µmol/kg	Griffiths, Joanna S	Potentiometric titration
26	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Griffiths, Joanna S	Calculated using CO2calc
27	Calcite saturation state	Omega Cal		Griffiths, Joanna S	Calculated using CO2calc
28	Aragonite saturation state	Omega Arg		Griffiths, Joanna S	Calculated using CO2calc
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3168 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 Type (study)	3 Species	4 Reg spec no	5 URL ref (WoRMS Aphia ID)	6 Exp duration [days]	7 Treat	8 Location	9 ID (Coral)	10 ID (Jar)	11 G. cf. index	12 TAG/dm [µg/g]	13 FA tot [µg/g]	14 Sterol [µg/g]	15 PLFA [µg/g]	16 Lipids tot [µg/g]	17 TPRT [µg/g]	18 Dry m [g]	19 Dry m [g] (soluble)	20 Resp O2 [µmol/g/h]	21 Inc dur [weeks]	22 Temp [°C]	23 Sal	24 pH (total scale, Potentiometric)	25 AT [µmol/kg] (Potentiometric titration)	26 pCO2water_SST_wet [µatm] (Calculated using CO2calc)	27 Omega Cal (Calculated using CO2calc)	28 Omega Arg (Calculated using CO2calc)	29 CSC flag (Calculated using seacarb afte...)	30 CO2 [µmol/kg] (Calculated using seacarb afte...)	31 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	32 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	33 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	34 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	35 DIC [µmol/kg] (Calculated using seacarb afte...)	36 Omega Arg (Calculated using seacarb afte...)	37 Omega Cal (Calculated using seacarb afte...)
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	30	4	48	47	25	15	95	230	302	159	32			13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	29	6	51	40	39	15	167	312	237	72	6			13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	42	6	97	85	48	15	134	379	270	134	6			13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	34	5	108	70	112	34	325	649	962	185	4			13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	38	5	19	11	33	14	83	160	507	79	6			13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	40	4	16	8	12	2	106	144	140	25	2			13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	31	5	25	12	59	15	169	280	150	168	14			13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	52	6	22	21	52	8	107	210	182	143	9			13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	32	7	304	46	30	14	254	648	207	278	21			13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	39	7	34	13	27	10	103	187	133	130	7			13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	36	8	160	80	80	10	238	568	119	125	6			13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	37	8	192	129	45	13	158	537	283	130	23			13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	28	9	10	3	5	9	45	72	53	155	7			13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	33	9	32	8	60	15	155	270	373	130	8			13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	50	7	7	117	56	26	132	338	443	239	32			13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	43	8	59	39	33	7	184	322	450	227	14			13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	51	9	3	33	33	11	91	171	320	153	11			13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	18	1	5	0	0	13	53	71	210	192	12			13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	13	1	0	9	16	7	132	164	114	89	9			13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	4	2	31	0	5	7	100	143	16	94	11			13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	8	2	6	4	13	6	16	45	376	111	5			13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	6	3	11	12	37	20	54	134	297	62	4			13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	23	1	7	23	23	0	362	415	101	105	6			13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	2	3	0	11	23	19	128	181	117	285	16			13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	20	10	15	9	32	10	388	454	142	177	10			13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	21	10	34	8	26	2	178	248	394	54	4			13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	19	11	20	13	20	8	97	158	165	44	4			13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	10	11	9	4	14	12	102	141	176	141	7			13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	12	12	0	0	44	18	224	286	54	92	4			13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	11	12	4	5	38	18	112	177	300	125	20			13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	1	1	24	20	43	21	142	250	126	312	39			13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	9	11	2	10	40	23	183	258	291	134	23			13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	3	12	8	10	14	13	79	124	149	208	13			13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	7	2	15	5	7	4	152	183	8	154	7			13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	24	11	16	11	22	23	332	404	365	103	14			13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	27	1	6	2	20	3	170	201	82	98	6			13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	30	4										-0.424046390	1	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	35	4										-0.462225482	1	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	29	6										-0.472968788	1	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	42	6										-0.299907306	1	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	34	5										-0.264384245	1	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	38	5										-0.159269017	1	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	40	4										-0.324036837	1	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	31	5										-0.520145696	1	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	52	6										-0.289851417	1	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	32	7										-1.460348064	1	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	39	7										-2.667304206	1	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	36	8										-0.281959706	1	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	37	8										-0.253841945	1	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	28	9										-0.405649642	1	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	33	9										-0.508063765	1	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	50	7										-0.299981404	1	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	43	8										-0.353633651	1	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	51	9										-0.277843746	1	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	18	1										-0.339870318	1	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	13	1										-0.742328415	1	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	4	2										-0.511839007	1	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	8	2										-1.156389358	1	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	6	3										-0.542665859	1	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	5	3										-0.471273958	1	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	23	1										-0.207713910	1	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	15	2										-0.679537871	1	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	2	3										-0.196562766	1	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	20	10										-0.127632679	1	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	21	10										-0.071119770	1	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	19	11										-0.521978598	1	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	10	11										-0.558604028	1	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	12	12										-1.341004538	1	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	11	12										-0.189164940	1	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	1	10										-0.458664105	1	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	9	11										-0.363417941	1	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	3	12										-0.189454657	1	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	30	4										-0.606041530	4	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	35	4										-0.287044130	4	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	29	6										-0.299933850	4	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	42	6										-0.232821340	4	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	34	5										-0.398678120	4	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	38	5										-0.547774240	4	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	40	4										-0.914207020	4	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	31	5										-0.280822920	4	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	high upwelling	52	6										-0.474881530	4	13	29.9	7.40	2075.6	2365.4	0.70	0.44	8	76.29	1860.35	1867.28	1999.64	30.53	2106.46	0.47	0.75
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	32	7										-1.910270680	4	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	39	7										-2.357642570	4	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	36	8										-0.430736520	4	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	37	8										-0.285618050	4	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	28	9										-0.471681180	4	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	33	9										-0.442028200	4	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	50	7										-0.269108900	4	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	43	8										-0.374771690	4	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Point_Arena_Cove	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	high upwelling	51	9										-0.345334520	4	13	29.8	7.79	2072.4	768.4	1.76	1.11	8	29.50	719.00	721.67	1896.11	70.88	1996.49	1.10	1.74
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	18	1										-0.192681290	4	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	13	1										-0.179829580	4	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	4	2										-0.173447830	4	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	8	2										-0.009681610	4	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	6	3										-0.749722060	4	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	5	3										-0.155952800	4	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	23	1										-0.295105740	4	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	15	2										-0.333314560	4	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	Low pH	low upwelling	2	3										-0.062771660	4	13	29.8	7.39	2073.8	2368.9	0.71	0.45	8	78.15	1904.78	1911.88	1999.77	29.76	2107.69	0.46	0.73
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	20	10										-0.104505160	4	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	21	10										-0.041091420	4	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	19	11										-0.501561860	4	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	10	11										-0.544073870	4	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	12	12										-0.610726440	4	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	11	12										-0.428916110	4	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	1	10										-0.187179480	4	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	9	11										-0.334393290	4	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82
Goleta	laboratory	Balanophyllia elegans (coral)	286920	marinespecies.org	29	High pH	low upwelling	3	12										-0.289006880	4	13	29.8	7.81	2072.8	802.3	1.71	1.08	8	28.07	684.03	686.58	1888.91	73.94	1990.92	1.15	1.82