Citation:

PANGAEA.920153

Name: Seawater carbonate chemistry and gene expression in the gills of juvenile turbot (Psetta maxima)
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Laboratory experiment; Nekton; North Atlantic; Other studied parameter or process; Pelagos; Psetta maxima; Single species; Temperate

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Abstract:

The data sets stem from a study which aimed to investigate the impact of chronic severe hypercapnia on the cellular stress response in the gills of juvenile turbot (Psetta maxima). The experiment was conducted in the so called respirometer, a recirculating aquaculture respirometer system (RARS) which allowed the persistent, stable manipulation of carbon dioxide concentrations. Juvenile turbot (Psetta maxima) where subjected to different levels of environmental hypercapnia (low: ~3000 μatm, medium: ~15000 μatm, high: ~25000 μatm). After eight weeks, fish where sacrificed and gill samples were taken for further histological and transcriptional analysis. For histological investigation of the gills, samples where fixed in 4% phosphate-buffered formalin and examined via a microscope equipped with a digital camera. In order to investigate changes in the transcriptome, a fraction of the gill samples was stored in RNAlater. Relative gene expression analysis was conducted via RT-qPCR, using a Fluidigm chip system. Calibrated normalized relative quantities (CNRQs) where assessed with the QBASE software. This included the evaluation of the most suitable combination of reference genes with the help of the GeNorm algorithm.

Keyword(s):

Animalia; Chordata; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Laboratory experiment; Nekton; North Atlantic; Other studied parameter or process; Pelagos; Psetta maxima; Single species; Temperate

Related to:

Hermann, Bernd Timo; Würtz, Sven; Vanselow, Klaus Heinrich; Schulz, Carsten; Stiller, Kevin Torben (2019): Divergent gene expression in the gills of juvenile turbot (Psetta maxima) exposed to chronic severe hypercapnia indicates dose-dependent increase in intracellular oxidative stress and hypoxia. Aquatic Toxicology, 206, 72-80, https://doi.org/10.1016/j.aquatox.2018.10.023

Original version:

Hermann, Bernd Timo; Würtz, Sven; Vanselow, Klaus Heinrich; Schulz, Carsten; Stiller, Kevin Torben (2018): Histological and relative gene expression data obtained from the gills of juvenile turbot (Psetta maxima) after the exposure to varying degrees of severe environmental hypercapnia for 8 weeks. PANGAEA, https://doi.org/10.1594/PANGAEA.893884

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Hermann, Bernd Timo		study
2	Species	Species		Hermann, Bernd Timo
3	Registration number of species	Reg spec no		Hermann, Bernd Timo
4	Uniform resource locator/link to reference	URL ref		Hermann, Bernd Timo		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Hermann, Bernd Timo
6	Sample type	Samp type		Hermann, Bernd Timo
7	Sample ID	Sample ID		Hermann, Bernd Timo
8	Treatment	Treat		Hermann, Bernd Timo
9	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Hermann, Bernd Timo
10	Replicate	Repl		Hermann, Bernd Timo
11	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Adenylat Kinase 2
12	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Akirin
13	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Apolipoprotein E
14	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Arginase
15	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Cathepsin D
16	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Copper Chaperone
17	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Cytochrome C Oxidase
18	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Cystatin B
19	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Cytochrome P450
20	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	delta_6-Fatty acid Desaturase
21	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Elongation Factor 1 alpha
22	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Polyunsaturated fatty acid elongase
23	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	growth arrest and DNA-damage inducible protein 45 alpha
24	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	growth hormone receptor
25	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Cell death-regulatory protein
26	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Glutathione-S-Transferase
27	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	3-Hydroxy-3Methylglutaryl-Coenzym A Reductase
28	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Heat Shock Protein 40kDa
29	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Heat Shock Protein 70kDa
30	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Heat Shock Protein 90kDa
31	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Insulin-like growth factor 1
32	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Insulin-like growth factor 2
33	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Interleukin-1 beta
34	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Interferone regulatory factor 3
35	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Interferone regulatory factor 5
36	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Interferone regulatory factor 7
37	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Low molecular weight polypeptide
38	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Lipoprotein-Lipase
39	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Lysocyme C
40	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Natural resistance associated macrophage protein
41	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Protein Phosphatase 1 catalytic subunit beta
42	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Peroxiredoxin 1
43	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Signal transductor and activator of transcription 2
44	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Transferrin
45	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Transferrin receptor
46	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Toll-like receptor 3
47	CNRQs, Quantitative reverse transcription PCR	RT-qPCR		Hermann, Bernd Timo	Quantitative reverse transcription polymerase chain reaction (qRT-PCR)	Tumor necrosis factor alpha
48	Gill, hypertrophied chloride cells	Gill HCC	%	Hermann, Bernd Timo	Microscopy
49	Gill, hyperplasia on basis of primary filament	Gill HbpF	%	Hermann, Bernd Timo	Microscopy
50	Gill, not affected	Gill NA	%	Hermann, Bernd Timo	Microscopy
51	Gill, hypertrophy of secondary lamellae	Gill HoSL	%	Hermann, Bernd Timo	Microscopy
52	Gill, hyperplasia on distal part of secondary lamellae	Gill HodPosL	%	Hermann, Bernd Timo	Microscopy
53	Gill, general hyperplasia	Gill GH	%	Hermann, Bernd Timo	Microscopy
54	Gill, lamellar clubbing	Gill LC	%	Hermann, Bernd Timo	Microscopy
55	Salinity	Sal		Hermann, Bernd Timo
56	Temperature, water	Temp	°C	Hermann, Bernd Timo
57	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Hermann, Bernd Timo
58	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Hermann, Bernd Timo
59	pH	pH		Hermann, Bernd Timo		NBS scale
60	pH, standard deviation	pH std dev	±	Hermann, Bernd Timo		NBS scale
61	Alkalinity, total	AT	µmol/kg	Hermann, Bernd Timo
62	Alkalinity, total, standard deviation	AT std dev	±	Hermann, Bernd Timo
63	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
65	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
66	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
67	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
68	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
69	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
70	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
71	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
72	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

4447 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Samp type	7 Sample ID	8 Treat	9 pCO2water_SST_wet [µatm]	10 Repl	11 RT-qPCR (Adenylat Kinase 2, Quantitati...)	12 RT-qPCR (Akirin, Quantitative reverse ...)	13 RT-qPCR (Apolipoprotein E, Quantitativ...)	14 RT-qPCR (Arginase, Quantitative revers...)	15 RT-qPCR (Cathepsin D, Quantitative rev...)	16 RT-qPCR (Copper Chaperone, Quantitativ...)	17 RT-qPCR (Cytochrome C Oxidase, Quantit...)	18 RT-qPCR (Cystatin B, Quantitative reve...)	19 RT-qPCR (Cytochrome P450, Quantitative...)	20 RT-qPCR (delta_6-Fatty acid Desaturase...)	21 RT-qPCR (Elongation Factor 1 alpha, Qu...)	22 RT-qPCR (Polyunsaturated fatty acid el...)	23 RT-qPCR (growth arrest and DNA-damage ...)	24 RT-qPCR (growth hormone receptor, Quan...)	25 RT-qPCR (Cell death-regulatory protein...)	26 RT-qPCR (Glutathione-S-Transferase, Qu...)	27 RT-qPCR (3-Hydroxy-3Methylglutaryl-Coe...)	28 RT-qPCR (Heat Shock Protein 40kDa, Qua...)	29 RT-qPCR (Heat Shock Protein 70kDa, Qua...)	30 RT-qPCR (Heat Shock Protein 90kDa, Qua...)	31 RT-qPCR (Insulin-like growth factor 1,...)	32 RT-qPCR (Insulin-like growth factor 2,...)	33 RT-qPCR (Interleukin-1 beta, Quantitat...)	34 RT-qPCR (Interferone regulatory factor...)	35 RT-qPCR (Interferone regulatory factor...)	36 RT-qPCR (Interferone regulatory factor...)	37 RT-qPCR (Low molecular weight polypept...)	38 RT-qPCR (Lipoprotein-Lipase, Quantitat...)	39 RT-qPCR (Lysocyme C, Quantitative reve...)	40 RT-qPCR (Natural resistance associated...)	41 RT-qPCR (Protein Phosphatase 1 catalyt...)	42 RT-qPCR (Peroxiredoxin 1, Quantitative...)	43 RT-qPCR (Signal transductor and activa...)	44 RT-qPCR (Transferrin, Quantitative rev...)	45 RT-qPCR (Transferrin receptor, Quantit...)	46 RT-qPCR (Toll-like receptor 3, Quantit...)	47 RT-qPCR (Tumor necrosis factor alpha, ...)	48 Gill HCC [%] (Microscopy)	49 Gill HbpF [%] (Microscopy)	50 Gill NA [%] (Microscopy)	51 Gill HoSL [%] (Microscopy)	52 Gill HodPosL [%] (Microscopy)	53 Gill GH [%] (Microscopy)	54 Gill LC [%] (Microscopy)	55 Sal	56 Temp [°C]	57 pCO2water_SST_wet [µatm]	58 pCO2 std dev [±]	59 pH (NBS scale)	60 pH std dev [±] (NBS scale)	61 AT [µmol/kg]	62 AT std dev [±]	63 CSC flag (Calculated using seacarb afte...)	64 pH (total scale, Calculated using...)	65 CO2 [µmol/kg] (Calculated using seacarb afte...)	66 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	67 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	68 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	69 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	70 DIC [µmol/kg] (Calculated using seacarb afte...)	71 Omega Arg (Calculated using seacarb afte...)	72 Omega Cal (Calculated using seacarb afte...)
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	1	Low pCO2	3000	1	0.922	0.916	1.170	1.803	1.400	0.777	1.062	0.689	1.484	0.979	0.670	1.532	0.884	0.649	0.771	0.254	1.516	0.978	1.002	1.186	0.820	0.852	0.286	0.842	0.662	0.612	0.484	0.824	0.452	1.020	0.210	0.793	1.118	0.393	1.313	0.350	0.561								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	100	High pCO2	25000	8	1.224	1.122	0.962	0.615	0.955	0.882	0.948	0.984	1.451	0.976	0.498	0.778	0.783	0.836	1.549	1.309	1.342	0.978	0.867	0.812	1.636	1.055	0.672	0.824	1.154	0.422	1.177	0.557	1.110	0.790	1.776	0.776	0.705	0.899	0.746	2.243	1.393								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	101	High pCO2	25000	8	1.068	1.136	1.101	0.632	0.556	0.784	0.641	1.085	1.098	1.551	0.621	0.575	1.112	1.088	1.298	0.904	0.854	0.989	0.558	0.652	1.175	1.334	0.740	0.967	0.906	0.897	1.128	0.406	0.333	0.506	2.173	1.086	0.422	0.995	1.269	1.675	1.957								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	102	High pCO2	25000	8	1.155	0.726	1.072	0.971	1.842	1.355	1.060	0.799	0.824	0.921	0.637	1.161	0.857	1.070	0.900	0.450	1.182	1.305	1.753	0.983	1.770	0.827	0.942	0.693	0.845	0.441	0.620	0.375	0.985	1.486	0.063	0.485	0.984	0.536	1.187	1.142	0.280								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	103	High pCO2	25000	8	1.378	1.133	1.587	0.823	0.728	0.966	0.948	1.253	0.699	1.265	0.661	0.716	0.690	0.428	1.196	0.739	1.168	0.921	0.728	0.833	0.414	0.480	2.585	1.008	0.956	0.944	1.635	0.811	0.474	0.676	1.909	1.320	0.744	0.988	0.816	0.634	1.455								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	113	High pCO2	25000	9	0.806	1.406	1.112	0.812	0.752	0.790	0.840	0.923	0.764	1.874	0.639	0.661	1.141	1.068	0.970	0.995	1.423	6.937	0.701	0.950	2.100	1.205	0.748	1.839	0.796	1.743	1.382	1.350	0.600	0.682	2.037	1.038	0.889	0.374	1.293	0.415	1.094								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	114	High pCO2	25000	9	0.968	0.797	0.959	1.189	1.127	0.988	0.960	0.665	1.584	1.529	1.012	1.030	0.807	0.935	0.906	0.836	1.567	0.693	1.120	0.921	1.457	0.952	0.718	0.809	0.905	1.086	0.799	0.823	0.638	0.966	1.479	0.639	1.187	0.314	0.708	0.424	1.157								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	115	High pCO2	25000	9	0.990	0.740	1.588	2.167	1.681	1.393	0.722	0.789	0.404	1.308	0.784	1.477	0.862	0.466	1.015	0.579	1.308	0.667	1.246	0.919	0.541	0.527	2.824	0.758	0.894	1.170	0.715	1.278	0.532	1.277	1.416	0.586	1.042	0.236	0.814	2.378	0.260								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	116	High pCO2	25000	9	0.767	1.211	1.000	0.737	0.950	1.144	0.646	0.994	0.935	1.295	0.852	1.287	1.186	0.738	0.985	0.971	0.985	0.757	0.730	0.775	0.876	0.851	1.487	1.057	1.100	1.742	1.704	1.393	1.052	0.918	1.878	0.946	0.914	1.268	0.519	0.699	1.038								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	117	High pCO2	25000	9	1.094	1.185	0.955	1.015	1.031	0.837	0.751	0.894	0.995	1.961	0.760	1.037	1.121	0.841	0.842	0.996	1.748	0.778	0.918	1.181	1.737	1.144	0.980	1.062	1.029	0.759	1.048	1.032	1.353	0.931	1.612	0.865	1.009	2.923	0.989	1.170	1.108								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	15	Low pCO2	3000	2	1.046	0.681	1.418	1.419	0.995	0.913	1.080	0.664	2.155	0.911	0.839	1.386	0.885	1.217	0.780	0.730	0.768	0.982	1.393	1.197	1.123	1.130	0.320	1.005	1.069	0.564	0.631	0.728	0.744	1.471	1.587	0.557	1.451	0.896	2.386	1.653	0.696								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	16	Low pCO2	3000	2	0.981	0.497	0.978	1.070	1.217	0.784	0.981		3.534	0.814	0.824	1.179	0.776		0.876	5.943	1.395		1.011	1.024	1.538		0.212		0.898		0.911	0.548	0.459	1.099	0.172		0.948	0.373		1.354	1.393								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	17	Low pCO2	3000	2	1.126	1.322	1.180	0.321	0.535	0.844	1.375	1.257	0.672	0.761	1.134	0.650	0.976	0.458	1.174	1.078	0.470	0.826	0.599	0.537	0.278	0.512	0.618	0.948	1.253	1.136	2.002	1.028	0.366	0.482	2.216	1.654	0.594	0.804	0.522	1.725	2.902								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	18	Low pCO2	3000	2	0.743	0.835	1.279	0.965	0.466	0.863	0.871	0.962	0.852	1.179	1.064	0.877	0.719	1.104	0.708	0.972	1.301	1.158	1.073	0.911	0.750	1.022	0.344	0.754	0.417	0.550	0.715	0.766	6.342	0.712	0.914	1.231	1.104	0.078	1.460	0.467	1.473								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	19	Low pCO2	3000	2	0.955	0.674	1.560	1.028	0.793	0.817	0.854	0.714	1.938	0.946	0.829	1.276	0.873	0.775	0.853	0.879	1.233	0.760	0.977	0.930	1.245	0.828	0.477	0.687	0.913	0.394	0.590	0.451	0.879	1.069	1.244	0.713	0.957	0.632	1.182	1.107	0.822								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	2	Low pCO2	3000	1	0.540	1.112	0.679	1.238	1.264	7.617	2.875	0.769	0.829	1.852	2.839	1.017	1.271	0.911	0.674	0.976	0.011	0.691	4.137	2.274	2.738	0.693	1.333	0.735	1.249	1.426	0.341	1.009	1.519	1.256	0.668	0.774	3.604	0.300	0.735	3.363	0.077								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	29	Low pCO2	3000	3	1.035	1.179	1.156	0.815	0.698	0.663	1.296	0.984	1.992	0.935	0.977	1.188	0.939	0.995	1.220	1.326	1.041	0.987	0.874	1.038	0.810	1.305	0.367	0.696	0.924	0.525	1.210	0.644	0.393	0.627	1.648	1.208	0.601	0.241	0.574	0.380	1.182								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	3	Low pCO2	3000	1	0.863	1.218	0.699	1.348	1.536	1.052	0.890	0.750	1.452	0.568	1.025	1.560	1.152	1.355	0.858	1.077	0.982	1.037	0.902	1.234	2.709	1.152	0.680	1.244	1.379	0.978	0.950	1.694	1.124	1.280	1.596	0.761	1.386	1.121	0.684	1.143	0.876								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	30	Low pCO2	3000	3	1.304	0.914	0.982	1.626	1.450	1.249	1.246	0.832	0.844	0.539	1.048	1.595	1.274	0.946	0.838	0.950	1.245	1.126	1.758	1.390	1.036	1.036	1.099	1.614	1.191	1.401	0.844	1.217	1.889	1.410	1.598	0.537	1.670	1.631	0.967	1.361	0.535								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	31	Low pCO2	3000	3	0.864	0.040	0.911	1.196	1.464	1.091	1.060	1.946	0.260	1.004	0.879	0.927	2.604	0.843	1.074	1.696	1.201	1.438	1.203	0.758	0.490	5.369	0.757	1.073	0.704	8.105	0.417	0.811	0.670	1.263	0.120	1.707	1.073	1.120	3.495	0.278	0.384								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	32	Low pCO2	3000	3	0.923	1.054	0.617	0.870	0.746	0.772	0.827	0.858	1.825	0.866	1.466	0.772	0.933	0.727	1.122	1.202	1.284	0.993	0.825	0.965	1.402	1.046	0.967	1.204	0.743	0.683	0.857	0.734	0.890	0.817	1.594	1.022	0.699	1.017	0.730	1.225	0.816								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	33	Low pCO2	3000	3	1.036	0.800	0.631	0.811	1.460	0.986	1.078	0.878	0.514	0.492	1.622	0.805	1.002	0.705	0.831	0.749	0.774	0.606	1.382	1.047	0.245	0.775	1.503	0.780	0.910	1.284	0.850	2.657	0.617	1.059	0.218	0.903	0.972	2.646	0.968	1.132	0.784								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	4	Low pCO2	3000	1	1.322	1.032	0.889	1.647	1.588	1.094	1.190	0.793	2.293	0.588	1.129	1.516	1.453	1.310	0.698	1.385	1.161	0.937	1.497	1.081	3.120	1.347	1.461	0.924	1.383	0.987	0.713	0.707	1.713	1.584	1.401	0.535	1.403	2.001	0.995	0.497	1.001								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	43	Medium pCO2	15000	4	0.938	0.919	0.715	1.189	0.796	0.716	0.823	1.313	0.477	1.173	1.497	0.544	1.071	0.883	1.021	0.859	1.982	0.774	0.774	1.041	1.158	0.770	1.094	0.726	1.313	1.379	1.233	1.448	2.283	1.086	1.938	0.981	0.741	3.297	1.739	1.497	2.264								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	44	Medium pCO2	15000	4	0.912	1.424	0.889	0.760	0.867	0.862	1.117	1.263	1.536	0.709	1.634	0.867	1.178	1.530	1.114	1.570	1.046	1.329	0.783	1.016	2.719	1.500	0.576	0.840	1.030	0.712	1.366	0.701	0.455	0.813	1.773	1.207	0.818	0.708	0.923	1.088	1.296								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	45	Medium pCO2	15000	4	0.918	1.583	0.844	0.530	0.641	0.859	1.148	1.298	1.062	1.084	0.886	0.391	0.973	1.077	1.052	1.211	0.498	1.565	0.409	0.483	1.227	0.667	1.724	1.665	0.905	1.649	1.554	0.986	0.975	0.500	0.388	1.411	0.800	0.277	0.614	0.678	1.882								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	46	Medium pCO2	15000	4	0.941	1.403	0.858	1.134	0.910	0.891	0.990	1.076	1.089	0.757	0.832	0.851	1.457	1.245	1.067	1.585	1.982	1.188	0.872	0.960	1.611	1.392	1.238	1.606	0.935	1.261	1.070	1.455	2.262	0.922	1.703	0.947	1.106	0.965	1.323	0.423	0.996								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	47	Medium pCO2	15000	4	0.888	1.222	0.813	0.959	0.449	7.376	6.488	1.040	0.945	0.821	5.587	1.982	0.898	0.845	1.193	1.088	0.265	0.926	4.301	4.525	1.154	0.882	1.574	1.098	0.855	0.954	0.855	0.672	1.494	1.009	0.877	1.173	3.620	0.954	1.241	9.317	1.199								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	5	Low pCO2	3000	1	0.906	0.999	0.805	1.185	0.915	0.867	1.157	1.211	0.542	0.717	1.413	0.991	1.201	1.271	0.867	0.964	1.596	0.684	1.006	1.186	1.117	0.926	1.357	0.708	1.022	1.080	1.010	1.404	0.685	0.863	2.023	1.143	0.879	0.670	1.339	0.375	1.629								20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	57	Medium pCO2	15000	5	1.230	1.438	1.064	0.976	0.955	0.732	0.959	0.772	2.209	1.152	0.888	1.038	1.059	0.950	0.882	1.348	1.822	1.101	1.122	1.110	0.818	1.290	0.384	1.342	0.997	0.644	1.420	0.730	0.638	0.974	1.390	0.898	0.981	0.606	1.364	0.455	1.073								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	58	Medium pCO2	15000	5	1.319	0.792	1.243	0.697	0.888	0.833	1.120	1.439	0.586	0.958	0.858	0.592	1.000	0.851	1.761	1.034	1.084	0.912	0.814	0.948	1.007	0.893	0.403	0.888	0.639	0.585	1.489	0.627	0.637	0.463	0.402	1.586	0.926	0.322	0.756	1.204	1.491								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	59	Medium pCO2	15000	5	0.897	0.782	0.676	1.742	1.458	2.797	1.146	0.706	0.586	0.494	1.934	2.824	0.935	1.019	0.786	0.781	0.205	0.582	3.035	1.706	1.580	1.049	0.598	0.728	1.298	0.875	0.380	2.076	1.172	1.157	0.014	0.687	2.960	0.386	0.724	2.275	0.017								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	60	Medium pCO2	15000	5	0.898	1.082	0.914	0.545	0.872	0.886	0.747	1.048	0.838	1.136	1.001	0.445	0.742	0.624	1.015	1.045	0.456	0.613	0.678	0.539	0.653	0.481	1.561	1.059	0.926	1.667	1.610	0.537	0.357	0.712	0.199	1.104	1.006	0.406	0.753	1.252	1.185								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	61	Medium pCO2	15000	5	0.755	1.426	0.796	1.381	0.922	0.905	0.984	1.209	0.444	0.409	1.311	0.825	1.344	0.878	0.954	0.923	0.809	1.240	0.955	1.020	1.248	1.166	1.569	1.060	0.978	0.979	1.360	3.915	0.542	1.068	1.968	1.176	1.038	0.744	0.777	1.920	1.139								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	71	Medium pCO2	15000	6	1.104	1.361	0.843	1.533	1.670	1.269	0.864	0.737	2.361	0.505	1.335	1.486	0.997	1.207	1.015	1.354	1.145	0.921	1.429	1.477	0.916	1.346	0.504	0.944	1.294	1.032	1.108	4.540	0.944	1.314	1.938	0.992	1.325	0.559	0.612	1.546	0.820								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	72	Medium pCO2	15000	6	1.093	1.506	1.134	1.073	1.001	0.836	0.897	0.858	2.395	1.348	0.923	0.886	0.968	0.775	0.993	1.154	1.349	1.290	0.878	0.993	0.508	1.171	1.087	1.661	1.094	0.973	1.255	1.081	0.317	0.918	1.660	1.133	1.286	0.504	0.705	0.438	1.229								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	73	Medium pCO2	15000	6	1.043	1.181	0.446	0.781	1.132	0.818	0.701	1.893	0.410	1.028	1.508	0.584	0.743	0.946	0.915	0.689	1.390	1.055	0.743	0.714	2.024	2.204	2.336	1.405	1.045	3.191	0.856	1.228	1.092	0.932	1.428	2.649	0.960	0.764	1.829	0.476	0.890								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	74	Medium pCO2	15000	6	0.944	1.331	1.055	1.228	1.278	0.814	1.247	1.105	0.455	0.749	0.739	1.119	1.437	0.857	1.166	1.064	1.765	1.227	1.316	1.185	0.421	1.180	1.898	1.231	1.170	1.340	1.513	1.637	5.354	1.212	2.133	1.005	1.156	2.654	0.926	1.290	2.278								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	75	Medium pCO2	15000	6	1.275	2.404	0.896	1.425	0.632	0.929	1.286	2.860	2.327	2.137	1.016	1.075	0.844	0.834	1.874	3.407	1.095	1.748	0.685	0.830	0.768	1.352	5.017	1.560	0.776	2.578	1.274	0.646	0.474	0.611	0.400	3.288	0.674	0.281	1.233	2.984	1.917								20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	85	High pCO2	25000	7	0.893	0.994	1.347	1.151	0.784	0.820	0.749	1.060	0.987	2.174	0.753	1.148	0.850	0.774	0.926	0.669	1.917	0.904	0.923	1.112	0.459	0.787	1.522	0.948	0.733	0.878	1.222	0.672	0.850	0.906	1.702	1.074	0.853	0.394	1.235	0.879	1.146								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	86	High pCO2	25000	7	0.765	0.750	1.124	0.684	0.665	0.673	0.654	0.863	1.131	1.427	0.816	0.817	0.699	0.682	0.996	0.649	1.410	0.727	0.714	0.966	0.516	0.783	0.938	0.881	0.899	0.683	1.013	0.474	0.177	0.686	1.469	0.961	0.878	0.469	1.316	1.060	1.217								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	87	High pCO2	25000	7	1.167	0.831	1.284	1.122	0.933	0.808	0.661	0.736	0.550	1.102	0.873	1.359	0.976	0.743	0.847	0.528	2.571	0.867	0.840	1.303	0.550	0.708	1.648	1.099	0.794	1.948	1.277	2.029	0.612	0.860	0.274	0.820	1.094	0.432	0.803	0.773	1.161								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	88	High pCO2	25000	7	1.289	1.078	2.355	0.406	0.634	0.838	0.820	1.224	0.538	1.537	0.675	0.680	1.170	0.578	1.086	1.008	1.179	1.157	0.815	0.601	0.426	0.611	1.664	1.026	1.059	0.449	0.925	0.346	3.017	0.677	1.672	0.846	0.496	1.022	0.755	1.079	0.910								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	89	High pCO2	25000	7	0.810	1.078	1.113	0.905	0.512	0.666	0.730	1.150	0.785	1.239	0.697	0.713	0.435	0.655	1.231	0.832	0.954	0.964	0.454	0.693	1.623	0.806	3.239	0.607	0.858	0.577	1.364	0.658	0.292	0.562	2.036	1.428	0.538	0.309	1.343	0.592	1.856								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	99	High pCO2	25000	8	1.064	1.255	1.288	1.046	1.183	0.779	0.871	0.964	1.375	1.224	0.751	0.961	1.265	1.514	1.116	1.225	1.931	1.100	0.919	0.838	1.530	1.625	0.680	1.286	0.834	0.936	1.281	0.832	0.671	0.784	0.312	1.041	0.810	0.814	1.308	0.612	1.297								20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	1	Low pCO2	3000																																							80	80	49.75	10.00	3.75	14.75	18.25	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	2	Low pCO2	3000																																							80	80	56.00	12.50	7.00	12.50	12.00	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	3	Low pCO2	3000																																							40	60	48.00	22.00	6.50	7.50	16.00	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	4	Low pCO2	3000																																							80	80	46.50	17.00	14.00	3.00	19.50	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	5	Low pCO2	3000																																							80	60	51.50	2.50	18.50	9.00	18.50	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	6	Low pCO2	3000																																							60	60	50.00	9.50	12.00	18.00	10.50	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	7	Low pCO2	3000																																							40	80	45.50	4.50	4.00	27.50	16.50	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	8	Low pCO2	3000																																							100	40	58.50	7.00	11.50	3.50	19.50	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	9	Low pCO2	3000																																							80	40	49.50	4.50	6.00	10.50	29.50	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	10	Low pCO2	3000																																							80	80	26.00	7.00	3.50	24.50	37.50	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	11	Low pCO2	3000																																							100	100	43.00	9.50	13.00	10.00	24.50	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	12	Low pCO2	3000																																							60	40	39.50	9.50	2.00	35.00	14.00	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	13	Low pCO2	3000																																							100	0	70.50	12.00	3.00	11.50	2.00	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	14	Low pCO2	3000																																							40	80	37.00	0.50	8.00	15.00	38.50	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	15	Low pCO2	3000																																							20	40	72.50	5.50	3.00	16.00	3.00	20	17.7	2924	179	7.36	0.00	2190	80	26	7.22	126.26	3361.67	3373.49	2144.10	19.67	2290.03	0.32	0.52
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	16	Medium pCO2	15000																																							100	80	9.50	2.50	1.00	30.00	57.00	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	17	Medium pCO2	15000																																							80	40	70.50	4.50	1.00	5.50	15.00	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	18	Medium pCO2	15000																																							40	80	32.00	8.00	6.00	34.50	18.50	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	19	Medium pCO2	15000																																							100	80	26.00	13.00	8.00	27.50	25.50	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	20	Medium pCO2	15000																																							80	60	40.50	11.50	14.00	14.50	13.00	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	21	Medium pCO2	15000																																							40	40	40.50	6.00	1.00	29.00	22.50	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	22	Medium pCO2	15000																																							20	60	56.50	3.25	1.00	5.00	28.50	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	23	Medium pCO2	15000																																							80	80	41.00	6.50	7.00	29.00	17.00	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	24	Medium pCO2	15000																																							0	20	65.50	2.50	6.00	17.50	8.00	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	25	Medium pCO2	15000																																							100	60	50.50	3.50	13.00	8.50	23.50	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	26	Medium pCO2	15000																																							80	60	51.50	10.50	10.00	20.00	8.50	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	27	Medium pCO2	15000																																							100	80	39.00	10.00	11.00	19.50	20.50	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	28	Medium pCO2	15000																																							60	60	40.50	18.50	6.00	30.00	5.50	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	29	Medium pCO2	15000																																							80	100	16.50	1.00	3.00	25.50	53.50	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	30	Medium pCO2	15000																																							80	60	60.00	2.00	4.00	22.00	12.00	20	17.7	15397	656	6.66	0.01	2210	80	26	6.52	649.68	17298.25	17360.01	2201.00	4.03	2854.71	0.06	0.11
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	31	High pCO2	25000																																							20	40	31.50	15.50	0.50	39.00	13.50	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	32	High pCO2	25000																																							100	80	41.50	13.00	4.50	35.00	6.00	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	33	High pCO2	25000																																							100	80	37.50	17.00	17.50	9.00	17.50	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	34	High pCO2	25000																																							80	60	43.00	9.50	8.50	20.50	17.00	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	35	High pCO2	25000																																							80	40	52.00	6.00	4.50	22.00	13.50	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	36	High pCO2	25000																																							60	0	56.50	8.50	6.00	13.50	12.00	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	37	High pCO2	25000																																							80	80	59.50	20.00	9.50	6.50	4.50	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	38	High pCO2	25000																																							100	60	33.50	12.00	2.50	32.50	18.50	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	39	High pCO2	25000																																							60	60	59.00	16.00	0.50	9.00	15.50	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	40	High pCO2	25000																																							80	60	53.00	12.00	4.50	18.00	12.50	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	41	High pCO2	25000																																							60	20	50.50	24.50	1.00	16.50	7.00	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	42	High pCO2	25000																																							100	100	29.00	13.00	1.50	25.00	31.50	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	43	High pCO2	25000																																							100	80	34.50	12.50	6.00	15.50	31.50	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	44	High pCO2	25000																																							100	100	33.50	24.00	10.50	13.00	18.00	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07
laboratory	Psetta maxima (fish)	154473	marinespecies.org	56	Gill Sample	45	High pCO2	25000																																							100	100	20.50	12.00	5.50	30.00	31.50	20	17.7	24826	1850	6.44	0.00	2250	90	26	6.30	1099.67	29279.66	29385.53	2244.91	2.48	3347.06	0.04	0.07