Citation:

PANGAEA.920009

Name: Seawater carbonate chemistry and expression of c-Fos protein in the brain of marine medaka
Published: 2020-07-10
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Behaviour; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; Nekton; North Pacific; Oryzias melastigma; Pelagos; Single species; Temperate

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Abstract:

Altered behaviors have been reported in many marine fish following exposure to high CO2 concentrations. However, the mechanistic link between elevated CO2 and activation of brain regions in fish is unknown. Herein, we examined the relative quantification and location of c-Fos expression in marine medaka following acute (360 min) and short-term (7 d) exposure to CO2-enriched water (1000 ppm and 1800 ppm CO2). In the control and two treatment groups, pH was stable at 8.21, 7.92 and 7.64, respectively. After acute exposure to seawater acidified by enrichment with CO2, there was a clear upregulation of c-Fos protein in the medaka brain (P < 0.05). c-Fos protein expression peaked after 120 min exposure in the two treatment groups and thereafter began to decline. There were marked increases in c-Fos-labeling in the ventricular and periventricular zones of the cerebral hemispheres and the medulla oblongata. After 1800 ppm CO2 exposure for 7 d, medaka showed significant preference for dark zones during the initial 2 min period. c-Fos protein expression in the ventricular and periventricular zones of the diencephalon in medaka exposed to 1000 ppm and 1800 ppm CO2 were 0.51 ± 0.10 and 1.34 ± 0.30, respectively, which were significantly higher than controls (P < 0.05). Highest doublecortin protein expression occurred in theventricular zones of the diencephalon and mesencephalon. These findings suggest that the ventricular and periventricular zones of the cerebral hemispheres and the medulla oblongata of marine medaka are involved in rapid acid-base regulation. Prolonged ocean acidification may induce cell mitosis and differentiation in the adult medaka brain.

Keyword(s):

Animalia; Behaviour; Bottles or small containers/Aquaria (<20 L); Chordata; Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; Nekton; North Pacific; Oryzias melastigma; Pelagos; Single species; Temperate

Supplement to:

Wang, Xiaojie; Lv, Yutao; Xie, Jinling; Li, Baolin; Zhou, Tangjian; Chen, Yaqi; Chen, Yi; Song, Jiakun (2020): Brain regions of marine medaka activated by acute and short-term ocean acidification. Science of the Total Environment, 720, 137279, https://doi.org/10.1016/j.scitotenv.2020.137279

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Wang, Xiaojie		study
2	Species	Species		Wang, Xiaojie
3	Registration number of species	Reg spec no		Wang, Xiaojie
4	Uniform resource locator/link to reference	URL ref		Wang, Xiaojie		WoRMS Aphia ID
5	Treatment	Treat		Wang, Xiaojie
6	Experiment duration	Exp duration	h	Wang, Xiaojie
7	Experiment duration	Exp duration	days	Wang, Xiaojie
8	Time in minutes	Time	min	Wang, Xiaojie
9	pH	pH		Wang, Xiaojie		NBS scale
10	pH, standard deviation	pH std dev	±	Wang, Xiaojie		NBS scale
11	Gene expression	Gene expression		Wang, Xiaojie		c-Fos protein
12	Gene expression, standard deviation	Gene expression std dev	±	Wang, Xiaojie		c-Fos protein
13	Time point, descriptive	Time point		Wang, Xiaojie
14	Proportion of time	Proportion of time		Wang, Xiaojie		in black zone
15	Proportion, standard deviation	Prop std dev	±	Wang, Xiaojie		in black zone
16	Speed, swimming	Sp swim	cm/s	Wang, Xiaojie
17	Speed, swimming, standard deviation	Sp swim std dev	±	Wang, Xiaojie
18	Gene expression	Gene expression		Wang, Xiaojie		c-Fos protein after exposure to acidified water for 7 d
19	Gene expression	Gene expression		Wang, Xiaojie		DCX protein after exposure to acidified water for 7 d
20	Salinity	Sal		Wang, Xiaojie
21	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Wang, Xiaojie	Calculated using CO2SYS
22	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Wang, Xiaojie	Calculated using CO2SYS
23	Temperature, water	Temp	°C	Wang, Xiaojie
24	Temperature, water, standard deviation	Temp std dev	±	Wang, Xiaojie
25	Alkalinity, total	AT	µmol/kg	Wang, Xiaojie	Potentiometric titration
26	Alkalinity, total, standard deviation	AT std dev	±	Wang, Xiaojie	Potentiometric titration
27	pH	pH		Wang, Xiaojie	Potentiometric	NBS scale
28	pH, standard deviation	pH std dev	±	Wang, Xiaojie	Potentiometric	NBS scale
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1337 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 Exp duration [h]	7 Exp duration [days]	8 Time [min]	9 pH (NBS scale)	10 pH std dev [±] (NBS scale)	11 Gene expression (c-Fos protein)	12 Gene expression std dev [±] (c-Fos protein)	13 Time point	14 Proportion of time (in black zone)	15 Prop std dev [±] (in black zone)	16 Sp swim [cm/s]	17 Sp swim std dev [±]	18 Gene expression (c-Fos protein after exposure ...)	19 Gene expression (DCX protein after exposure to...)	20 Sal	21 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	22 pCO2 std dev [±] (Calculated using CO2SYS)	23 Temp [°C]	24 Temp std dev [±]	25 AT [µmol/kg] (Potentiometric titration)	26 AT std dev [±] (Potentiometric titration)	27 pH (NBS scale, Potentiometric)	28 pH std dev [±] (NBS scale, Potentiometric)	29 CSC flag (Calculated using seacarb afte...)	30 pH (Calculated using seacarb afte...)	31 CO2 [µmol/kg] (Calculated using seacarb afte...)	32 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	33 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	34 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	35 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	36 DIC [µmol/kg] (Calculated using seacarb afte...)	37 Omega Arg (Calculated using seacarb afte...)	38 Omega Cal (Calculated using seacarb afte...)
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		0	8.21	0.05										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		15	8.02	0.03										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		30	7.68	0.02										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		45	7.65	0.04										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		60	7.64	0.03										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		75	7.64	0.01										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		90	7.64	0.01										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		105	7.65	0.01										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		120	7.64	0.02										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		240	7.67	0.02										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		360	7.64	0.01										31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		0	8.20	0.03										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		15	8.10	0.02										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		30	7.90	0.02										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		45	7.89	0.03										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		60	7.90	0.01										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		75	7.90	0.02										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		90	7.91	0.01										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		105	7.90	0.01										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		120	7.91	0.02										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		240	7.92	0.02										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		360	7.91	0.01										31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		0			0.186	0.01								31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		30			0.217	0.03								31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		60			0.353	0.03								31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		120			1.357	0.04								31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm	6		360			0.991	0.01								31	987	78	24.4	0.02	2537	121	7.90	0.03	15	7.76	28.69	974.47	977.61	2241.48	124.25	2394.42	2.01	3.08
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		0			0.461	0.01								31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		30			0.697	0.02								31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		60			1.192	0.04								31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		120			1.782	0.05								31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm	6		360			1.249	0.02								31	1920	102	24.2	0.01	2575	98	7.64	0.02	15	7.50	56.13	1896.88	1903.03	2402.55	72.71	2531.38	1.18	1.80
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	Control		7						0-1 min	0.52	0.11	1.73	0.78			31	440	24	25.3	0.40	2578	124	8.21	0.03	15	8.07	12.50	434.43	435.81	2019.82	235.20	2267.52	3.82	5.85
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	Control		7						1-2 min	0.39	0.15	2.26	1.10			31	440	24	25.3	0.40	2578	124	8.21	0.03	15	8.07	12.50	434.43	435.81	2019.82	235.20	2267.52	3.82	5.85
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	Control		7						2-3 min	0.46	0.17	2.40	1.16			31	440	24	25.3	0.40	2578	124	8.21	0.03	15	8.07	12.50	434.43	435.81	2019.82	235.20	2267.52	3.82	5.85
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	Control		7						3- 6 min	0.51	0.12	2.32	0.48			31	440	24	25.3	0.40	2578	124	8.21	0.03	15	8.07	12.50	434.43	435.81	2019.82	235.20	2267.52	3.82	5.85
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	Control		7						6- 10 min	0.47	0.15	1.98	0.65			31	440	24	25.3	0.40	2578	124	8.21	0.03	15	8.07	12.50	434.43	435.81	2019.82	235.20	2267.52	3.82	5.85
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	Control		7						total	0.48	0.07	2.21	0.56			31	440	24	25.3	0.40	2578	124	8.21	0.03	15	8.07	12.50	434.43	435.81	2019.82	235.20	2267.52	3.82	5.85
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm		7						0-1 min	0.73	0.12	1.64	0.97			31	1950	113	25.2	0.50	2614	112	7.64	0.05	15	7.50	56.05	1943.38	1949.60	2434.23	76.04	2566.32	1.24	1.89
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm		7						1-2 min	0.55	0.18	2.33	1.50			31	1950	113	25.2	0.50	2614	112	7.64	0.05	15	7.50	56.05	1943.38	1949.60	2434.23	76.04	2566.32	1.24	1.89
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm		7						2-3 min	0.41	0.22	2.72	1.52			31	1950	113	25.2	0.50	2614	112	7.64	0.05	15	7.50	56.05	1943.38	1949.60	2434.23	76.04	2566.32	1.24	1.89
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm		7						3- 6 min	0.50	0.10	2.47	0.73			31	1950	113	25.2	0.50	2614	112	7.64	0.05	15	7.50	56.05	1943.38	1949.60	2434.23	76.04	2566.32	1.24	1.89
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm		7						6- 10 min	0.43	0.10	2.15	0.79			31	1950	113	25.2	0.50	2614	112	7.64	0.05	15	7.50	56.05	1943.38	1949.60	2434.23	76.04	2566.32	1.24	1.89
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm		7						total	0.51	0.09	2.30	0.78			31	1950	113	25.2	0.50	2614	112	7.64	0.05	15	7.50	56.05	1943.38	1949.60	2434.23	76.04	2566.32	1.24	1.89
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	control		7											0.145	0.34	31	440	24	25.3	0.40	2578	124	8.21	0.03	15	8.07	12.50	434.43	435.81	2019.82	235.20	2267.52	3.82	5.85
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	1000 ppm		7											0.510	0.75	31	956	55	24.7	0.60	2586	130	7.92	0.03	15	7.78	27.63	945.90	948.94	2270.59	133.05	2431.27	2.16	3.31
laboratory	Oryzias melastigma (fish)	315132	marinespecies.org	2000 ppm		7											1.340	1.16	31	1950	113	25.2	0.50	2614	112	7.64	0.05	15	7.50	56.05	1943.38	1949.60	2434.23	76.04	2566.32	1.24	1.89