Citation:

PANGAEA.919895

Name: Seawater carbonate chemistry and physiology and functional gene expression of the coccolithophore Emiliania huxleyi
Published: 2020-07-07
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Gene expression (incl. proteomics); Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Macro-nutrients; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature

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Abstract:

Here, we examined two-way and multiple driver effects of ocean acidification and other key environmental drivers - nitrate, phosphate, irradiance, and temperature - on the growth, photosynthetic and calcification rates, and the elemental composition of E. huxleyi. In addition, changes in functional gene expression were examined to understand the molecular mechanisms underpinning the physiological responses. The single driver manipulation experiments sugguest decreased nitrate supply being the most important driver regulating E. huxleyi physiology, by significantly reducing the growth, photosynthetic and calcification rates. In addition, the interaction of ocean acidification and decreased nitrate supply (projected for year 2100) had more negative synergistic effects on E. huxleyi physiology than all other two-way factorial manipulations, suggesting a linkage between the single dominant driver (nitrate) effects and interactive effects with other drivers. Simultaneous manipulation of all five environmental drivers to the projected year 2100 conditions had the largest negative effects on most of the physiological metrics. Furthermore, functional genes associated with inorganic carbon acquisition (RubisCO, AEL1 and delta CA) and calcification (CAX3, AEL1, PATP and NhaA2) were most down-regulated by the multiple driver manipulation, revealing linkages between responses of functional gene expression and associated physiological metrics.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Chromista; Emiliania huxleyi; Gene expression (incl. proteomics); Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Macro-nutrients; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature

Supplement to:

Feng, Yuanyuan; Roleda, Michael Y; Armstrong, Evelyn; Summerfield, Tina C; Law, Cliff S; Hurd, Catriona L; Boyd, Philip W (2020): Effects of multiple drivers of ocean global change on the physiology and functional gene expression of the coccolithophore Emiliania huxleyi. Global Change Biology, 26, 5630–5645, https://doi.org/10.1111/gcb.15259

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-07-07.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Feng, Yuanyuan		study
2	Species	Species		Feng, Yuanyuan
3	Registration number of species	Reg spec no		Feng, Yuanyuan
4	Uniform resource locator/link to reference	URL ref		Feng, Yuanyuan		WoRMS Aphia ID
5	Bottle number	Bottle	#	Feng, Yuanyuan
6	Treatment	Treat		Feng, Yuanyuan
7	Growth rate	µ	1/day	Feng, Yuanyuan		average last 5 days
8	Net photosynthesis rate, per cell	PN/cell	pg/#/h	Feng, Yuanyuan
9	Calcification rate of carbon per cell	Calc rate C/cell	pg/#/h	Feng, Yuanyuan
10	Ratio	Ratio		Feng, Yuanyuan		Photosynthetic/Calcification
11	Carbon, organic, particulate, per cell	POC/cell	pg/#	Feng, Yuanyuan
12	Carbon, inorganic, particulate, per cell	PIC/cell	pg/#	Feng, Yuanyuan
13	Particulate organic nitrogen per cell	PON/cell	pg/#	Feng, Yuanyuan
14	Phosphorus, organic, particulate, per cell	POP/cell	pg/#	Feng, Yuanyuan
15	Chlorophyll a per cell	Chl a/cell	pg/#	Feng, Yuanyuan
16	Gene expression, fold change, relative	Gene expression		Feng, Yuanyuan		alpha CA
17	Gene expression, fold change, relative, standard deviation	Gene expression std dev	±	Feng, Yuanyuan		alpha CA
18	Gene expression, fold change, relative	Gene expression		Feng, Yuanyuan		gamma CA
19	Gene expression, fold change, relative, standard deviation	Gene expression std dev	±	Feng, Yuanyuan		gamma CA
20	Gene expression, fold change, relative	Gene expression		Feng, Yuanyuan		delta CA
21	Gene expression, fold change, relative, standard deviation	Gene expression std dev	±	Feng, Yuanyuan		delta CA
22	Gene expression, fold change, relative	Gene expression		Feng, Yuanyuan		CAM
23	Gene expression, fold change, relative, standard deviation	Gene expression std dev	±	Feng, Yuanyuan		CAM
24	Gene expression, fold change, relative	Gene expression		Feng, Yuanyuan		CAX3
25	Gene expression, fold change, relative, standard deviation	Gene expression std dev	±	Feng, Yuanyuan		CAX3
26	Gene expression, fold change, relative	Gene expression		Feng, Yuanyuan		AEL_1
27	Gene expression, fold change, relative, standard deviation	Gene expression std dev	±	Feng, Yuanyuan		AEL_1
28	Gene expression, fold change, relative	Gene expression		Feng, Yuanyuan		GPA
29	Gene expression, fold change, relative, standard deviation	Gene expression std dev	±	Feng, Yuanyuan		GPA
30	Gene expression, fold change, relative	Gene expression		Feng, Yuanyuan		NhaA
31	Gene expression, fold change, relative, standard deviation	Gene expression std dev	±	Feng, Yuanyuan		NhaA
32	Gene expression, fold change, relative	Gene expression		Feng, Yuanyuan		PAPT
33	Gene expression, fold change, relative, standard deviation	Gene expression std dev	±	Feng, Yuanyuan		PAPT
34	Gene expression, fold change, relative	Gene expression		Feng, Yuanyuan		RibisCO
35	Gene expression, fold change, relative, standard deviation	Gene expression std dev	±	Feng, Yuanyuan		RibisCO
36	Salinity	Sal		Feng, Yuanyuan
37	Temperature, water	Temp	°C	Feng, Yuanyuan
38	Alkalinity, total	AT	µmol/kg	Feng, Yuanyuan
39	Carbon, inorganic, dissolved	DIC	µmol/kg	Feng, Yuanyuan
40	pH	pH		Feng, Yuanyuan		total scale
41	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Feng, Yuanyuan
42	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Feng, Yuanyuan
43	Bicarbonate ion	[HCO3]-	µmol/kg	Feng, Yuanyuan
44	Carbonate ion	[CO3]2-	µmol/kg	Feng, Yuanyuan
45	Carbon dioxide	CO2	µmol/kg	Feng, Yuanyuan
46	Calcite saturation state	Omega Cal		Feng, Yuanyuan
47	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
49	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

776 data points

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