Citation:

PANGAEA.919321

Name: Seawater carbonate chemistry and somatic and otolith growth relationship of Symphodus ocellatus
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Chordata; CO2 vent; Field observation; Growth/Morphology; Mediterranean Sea; Nekton; Pelagos; Single species; Symphodus ocellatus; Temperate

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Abstract:

Ocean acidification (OA) may have varied effects on fish eco-physiological responses. Most OA studies have been carried out in laboratory conditions without considering the in situ pCO2/pH variability documented for many marine coastal ecosystems. Using a standard otolith ageing technique, we assessed how in situ ocean acidification (ambient, versus end-of-century CO2 levels) can affect somatic and otolith growth, and their relationship in a coastal fish. Somatic and otolith growth rates of juveniles of the ocellated wrasse Symphodus ocellatus living off a Mediterranean CO2 seep increased at the high-pCO2 site. Also, we detected that slower-growing individuals living at ambient pCO2 levels tend to have larger otoliths at the same somatic length (i.e. higher relative size of otoliths to fish body length) than faster-growing conspecifics living under high pCO2 conditions, with this being attributable to the so-called 'growth effect'. Our findings suggest the possibility of contrasting OA effects on fish fitness, with higher somatic growth rate and possibly higher survival associated with smaller relative size of otoliths that could impair fish auditory and vestibular sensitivity.

Keyword(s):

Animalia; Chordata; CO2 vent; Field observation; Growth/Morphology; Mediterranean Sea; Nekton; Pelagos; Single species; Symphodus ocellatus; Temperate

Supplement to:

Di Franco, Antonio; Calo, Antonio; Sdiri, Khalil; Cattano, Carlo; Milazzo, Marco; Guidetti, Paolo (2019): Ocean acidification affects somatic and otolith growth relationship in fish: evidence from an in situ study. Biology Letters, 15(2), 20180662, https://doi.org/10.1098/rsbl.2018.0662

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 38.417520 * Longitude: 14.959970

Date/Time Start: 2012-05-01T00:00:00 * Date/Time End: 2013-06-30T00:00:00

Event(s):

Baia_di_Levante * Latitude: 38.417520 * Longitude: 14.959970 * Date/Time Start: 2012-05-01T00:00:00 * Date/Time End: 2013-06-30T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-06-12.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Di Franco, Antonio		study
2	Species	Species		Di Franco, Antonio
3	Registration number of species	Reg spec no		Di Franco, Antonio
4	Uniform resource locator/link to reference	URL ref		Di Franco, Antonio		WoRMS Aphia ID
5	Sampling date	Sampling date		Di Franco, Antonio
6	Site	Site		Di Franco, Antonio
7	Identification	ID		Di Franco, Antonio		Individual
8	Length, total	TL	mm	Di Franco, Antonio
9	Length, total	TL	mm	Di Franco, Antonio		at settlement back-calculated
10	Time in days	Time	days	Di Franco, Antonio		Pelagic larval duration
11	Age	Age	days	Di Franco, Antonio		Post-settlemnet
12	Age	Age	days	Di Franco, Antonio		Total
13	Time in days	Time	days	Di Franco, Antonio		Spawning date (ordinal, from the first date recorded)
14	Time in days	Time	days	Di Franco, Antonio		Settlement date (ordinal, from the first date recorded)
15	pH	pH		Di Franco, Antonio	Potentiometric	NBS scale
16	pH, standard deviation	pH std dev	±	Di Franco, Antonio	Potentiometric	NBS scale
17	Alkalinity, total	AT	µmol/kg	Di Franco, Antonio	Potentiometric titration
18	Salinity	Sal		Di Franco, Antonio
19	Salinity, standard deviation	Sal std dev	±	Di Franco, Antonio
20	Temperature, water	Temp	°C	Di Franco, Antonio
21	Temperature, water, standard deviation	Temp std dev	±	Di Franco, Antonio
22	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Di Franco, Antonio	Calculated using CO2SYS
23	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Di Franco, Antonio	Calculated using CO2SYS
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Di Franco, Antonio	Calculated using CO2SYS	20h
25	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Di Franco, Antonio	Calculated using CO2SYS
26	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Di Franco, Antonio	Calculated using CO2SYS	20h min
27	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Di Franco, Antonio	Calculated using CO2SYS	20h max
28	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
30	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3663 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Sampling date	6 Site	7 ID (Individual)	8 TL [mm]	9 TL [mm] (at settlement back-calculated)	10 Time [days] (Pelagic larval duration)	11 Age [days] (Post-settlemnet)	12 Age [days] (Total)	13 Time [days] (Spawning date (ordinal, from ...)	14 Time [days] (Settlement date (ordinal, fro...)	15 pH (NBS scale, Potentiometric)	16 pH std dev [±] (NBS scale, Potentiometric)	17 AT [µmol/kg] (Potentiometric titration)	18 Sal	19 Sal std dev [±]	20 Temp [°C]	21 Temp std dev [±]	22 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	23 pCO2 std dev [±] (Calculated using CO2SYS)	24 pCO2water_SST_wet [µatm] (20h, Calculated using CO2SYS)	25 pCO2 std dev [±] (Calculated using CO2SYS)	26 pCO2water_SST_wet [µatm] (20h min, Calculated using CO2SYS)	27 pCO2water_SST_wet [µatm] (20h max, Calculated using CO2SYS)	28 CSC flag (Calculated using seacarb afte...)	29 pH (total scale, Calculated using...)	30 CO2 [µmol/kg] (Calculated using seacarb afte...)	31 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	32 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	33 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	34 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	35 DIC [µmol/kg] (Calculated using seacarb afte...)	36 Omega Arg (Calculated using seacarb afte...)	37 Omega Cal (Calculated using seacarb afte...)
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	1	17.0	6.101	12.0	29.5	41.5	13	15	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	high-pCO2	2	25.0	6.030	6.5	32.0	38.5	18	14	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	3	19.0	6.095	8.0	27.0	35.0	20	18	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	high-pCO2	4	25.0	6.084	9.5	42.0	51.5	4	4	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	high-pCO2	5	22.0	7.002	11.0	28.5	39.5	16	18	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	high-pCO2	6	23.0	7.088	14.5	30.0	44.5	12	16	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	7	18.0	5.629	10.5	24.0	34.5	21	21	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	8	25.0	7.671	11.0	31.5	42.5	13	13	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	9	21.0	5.790	7.0	30.5	37.5	17	15	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	10	26.0	6.913	8.0	26.5	34.5	21	19	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	11	27.0	6.561	10.5	42.0	52.5	3	3	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	12	22.0	6.049	10.0	33.0	43.0	12	12	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	13	25.0	6.688	9.5	44.5	54.0	1	1	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	14	18.0	5.613	9.0	33.0	42.0	13	12	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	15	18.0	6.050	8.5	29.0	37.5	17	16	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	16	17.0	5.645	9.0	28.5	37.5	17	17	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	17	21.0	6.433	11.5	31.0	42.5	13	14	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	18	24.0	6.475	11.0	30.0	41.0	14	15	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	19	25.0	6.356	9.0	34.0	43.0	12	11	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	20	25.0	6.518	10.0	30.0	40.0	15	15	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	21	24.0	5.912	9.0	32.0	41.0	14	13	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	22	20.0	6.258	11.5	30.5	42.0	13	15	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	23	17.0	5.311	10.0	31.5	41.5	13	13	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	24	23.5	6.454	11.0	34.5	45.5	9	11	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	25	13.0	5.948	10.5	19.5	30.0	25	25	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	26	22.0	5.869	10.0	31.0	41.0	14	14	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	27	20.0	5.804	8.0	37.5	45.5	9	7	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	28	21.5	6.455	12.5	29.5	42.0	13	15	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	29	21.0	5.609	10.0	30.0	40.0	15	15	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	30	22.0	6.555	9.0	35.0	44.0	11	10	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	31	23.5	6.135	12.0	29.5	41.5	13	15	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	32	26.0	6.402	11.0	33.0	44.0	11	12	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	33	23.5	5.626	9.5	34.5	44.0	11	11	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	34	21.0	5.997	12.0	30.0	42.0	13	15	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	35	23.0	5.442	9.5	42.0	51.5	3	3	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	36	25.0	6.564	13.5	31.5	45.0	10	13	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	37	15.5	6.378	12.5	25.0	37.5	17	20	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	38	16.0	6.275	11.5	25.0	36.5	19	20	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	39	21.0	6.549	12.5	29.0	41.5	13	16	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	40	22.0	7.400	10.0	27.0	37.0	18	18	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	41	27.0	6.483	11.0	29.0	40.0	15	16	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	42	24.0	6.302	10.0	27.0	37.0	18	18	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	43	20.0	5.975	10.5	34.0	44.5	11	11	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-29	high-pCO2	44	24.0	6.549	13.0	30.5	43.5	11	15	7.94	0.09	2484	38.2	0.09	27.4	0.2	834	188	752	221	429	1407	26	7.80	21.60	820.58	823.14	2078.09	166.36	2266.05	2.61	3.91
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	1	21.5	6.145	8.0	35.5	43.5	10	8	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	2	20.0	6.251	12.0	24.5	36.5	18	20	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	3	13.0	5.650	9.5	22.0	31.5	22	22	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	4	15.0	5.120	10.0	30.5	40.5	14	14	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	5	21.0	6.225	8.0	26.5	34.5	20	18	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	6	14.0	5.379	11.0	26.0	37.0	17	18	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	7	21.5	6.253	7.5	29.5	37.0	17	14	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	8	16.0	5.415	7.5	26.0	33.5	20	18	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	9	23.0	7.024	13.0	32.0	45.0	9	12	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	ambient-pCO2	10	21.0	6.308	10.5	27.0	37.5	18	19	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	ambient-pCO2	11	17.0	5.948	10.5	29.0	39.5	16	17	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	ambient-pCO2	12	18.0	5.214	8.0	32.5	40.5	16	14	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	ambient-pCO2	13	25.0	7.086	11.5	36.5	48.0	8	10	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	14	18.5	5.481	9.0	34.0	43.0	11	10	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	15	20.0	5.515	10.5	36.0	46.5	8	8	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	16	17.5	5.238	10.0	30.5	40.5	14	14	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	17	19.0	5.635	9.0	28.0	37.0	17	16	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	18	20.0	6.862	9.5	28.5	38.0	16	16	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	19	16.5	5.641	11.0	29.5	40.5	14	14	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	20	19.0	5.677	9.5	33.0	42.5	12	11	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	21	17.0	5.251	9.0	35.0	44.0	10	9	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	22	19.5	5.287	8.5	34.5	43.0	11	10	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	23	20.0	5.455	9.5	34.0	43.5	10	10	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	24	17.5	6.129	9.5	30.0	39.5	14	14	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	25	20.0	5.989	11.5	31.0	42.5	12	13	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	26	17.0	5.329	8.5	34.0	42.5	12	10	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	27	17.5	6.028	9.0	32.0	41.0	13	12	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	28	20.0	5.808	9.5	33.0	42.5	12	11	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	29	24.0	5.764	10.0	40.0	50.0	4	4	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	30	19.0	6.049	10.5	31.0	41.5	12	13	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	31	19.0	6.002	11.0	30.5	41.5	12	14	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	32	18.0	5.678	10.5	32.5	43.0	11	12	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	33	17.0	5.837	9.0	24.0	33.0	21	20	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	34	21.0	5.570	9.5	37.0	46.5	8	7	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	35	18.0	5.623	12.5	31.5	44.0	10	12	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	36	18.0	6.334	10.0	28.5	38.5	16	16	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	37	22.0	5.433	9.5	32.5	42.0	12	12	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-27	ambient-pCO2	38	18.0	5.790	12.0	26.0	38.0	15	17	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-27	ambient-pCO2	39	16.0	6.209	12.0	18.0	30.0	23	25	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	40	18.0	5.592	8.5	33.0	41.5	12	11	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	41	19.0	5.486	9.5	33.5	43.0	11	10	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	42	15.0	6.231	13.0	23.5	36.5	18	20	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	43	17.0	5.832	10.0	27.5	37.5	16	16	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	44	12.0	5.279	11.0	23.5	34.5	20	20	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	45	16.5	5.593	10.5	34.0	44.5	10	10	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	46	17.0	6.185	10.0	24.5	34.5	20	20	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	47	17.5	5.198	10.5	33.5	44.0	10	10	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	48	16.5	6.040	11.0	26.0	37.0	17	18	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	49	18.0	5.480	8.5	28.0	36.5	18	16	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-28	ambient-pCO2	50	21.0	5.555	9.0	37.0	46.0	8	7	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	ambient-pCO2	51	23.0	6.199	9.0	33.5	42.5	14	12	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	ambient-pCO2	52	16.0	5.464	8.5	30.5	39.0	17	16	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	ambient-pCO2	53	15.0	7.232	15.0	19.0	34.0	22	27	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	ambient-pCO2	54	24.0	5.417	9.0	41.0	50.0	6	5	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58
field	Symphodus ocellatus (fish)	127460	marinespecies.org	2013-07-30	ambient-pCO2	55	18.0	5.144	7.0	38.5	45.5	10	8	8.13	0.03	2507	38.2	0.06	27.2	0.3	493	39	428	47	333	530	26	7.99	12.97	490.18	491.71	1927.11	237.50	2177.58	3.72	5.58