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Tornabene, Brian J; Breuner, Creagh W (2020): Morphology and corticosterone release rates of larval northern leopard frogs (Rana pipiens) per day throughout chronic NaCl exposure experiment [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.919205, In: Tornabene, BJ; Breuner, CW (2020): Morphology, survival and corticosterone release rates of larval northern leopard frogs throughout chronic NaCl exposure experiment [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.923785

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Published: 2020-06-19DOI registered: 2022-08-09

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Abstract:
We investigated the influence of exposure to chronic salinity (NaCl) and mifepristone (a glucocorticoid antagonist) for 24 d on corticosterone release rates (baseline, stress-induced and corticosterone increase) of Northern Leopard Frogs (Rana pipiens). The dataset contains per-individual, per-day values for snout-vent length (mm), wet mass (g), developmental stage (Gosner stage), log-transformed corticosterone release rates (baseline, stress-induced, and corticosterone increase; pg/h), and mass-corrected and log-transformed release rates (pg/g/h). It also contains coefficients of determination (%) for each corticosterone release rates calculated following quantification with enzyme immunoassays. Within the dataset, 'Died' indicates if that larvae subsequently died in the experiment
Keyword(s):
Anthropogenic disturbances; conservation physiology; mechanism; salt; stress
Related to:
Tornabene, Brian J; Hossack, Blake R; Crespi, Erica J; Breuner, Creagh W (2021): Corticosterone mediates a growth-survival tradeoff for an amphibian exposed to increased salinity. 335(8), 703-715, https://doi.org/10.1002/jez.2535
Coverage:
Date/Time Start: 2018-01-07T00:00:00 * Date/Time End: 2018-11-06T00:00:00
Event(s):
Rana_pipiens * Method/Device: Experiment (EXP)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Sample IDSample IDTornabene, Brian J
2ReplicateReplTornabene, Brian J
3Died yes/noDiedy/nTornabene, Brian JDuring experiment
4Treatment: hormonesT:hormonesTornabene, Brian JRU486 SKU M8046, Sigma AldrichMifepristone
5Treatment: NaClT:NaClmg/lTornabene, Brian JChloride titration strips, Hach
6DATE/TIMEDate/TimeTornabene, Brian JGeocode
7DayDayTornabene, Brian J
8StageStageTornabene, Brian JGosner staging tables
9MassMassgTornabene, Brian JDigital scale
10Snout-vent lengthSVLmmTornabene, Brian JCaliper
11Corticosterone release rate, mass-correctedC21H30O4 rel m corrpg/g/hTornabene, Brian JCorticosterone enzyme immunoassays (Cayman Chemical)Log-transformed and mass-corrected baseline cort release rate
12Corticosterone release rateC21H30O4 relpg/hTornabene, Brian JCorticosterone enzyme immunoassays (Cayman Chemical)Log-transformed baseline cort release rate
13Corticosterone release rate, mass-correctedC21H30O4 rel m corrpg/g/hTornabene, Brian JCorticosterone enzyme immunoassays (Cayman Chemical)Log-transformed and mass-corrected stress-induced cort release rate
14Corticosterone release rateC21H30O4 relpg/hTornabene, Brian JCorticosterone enzyme immunoassays (Cayman Chemical)Log-transformed stress-induced cort release rate
15Corticosterone increase, mass-correctedC21H30O4 incr m corrpg/g/hTornabene, Brian JCorticosterone enzyme immunoassays (Cayman Chemical)
16Corticosterone increaseC21H30O4 incrpg/hTornabene, Brian JCorticosterone enzyme immunoassays (Cayman Chemical)
17Coefficient of determinationR**2Tornabene, Brian JCalculatedBaseline cort coeffcient of variance (%)
18Coefficient of determinationR**2Tornabene, Brian JCalculatedStress-induced cort coefficient of variance (%)
19Coefficient of determinationR**2Tornabene, Brian JCalculatedCort increase coefficient of variance (%)
Size:
2556 data points

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