Sokolova, Inna; Falfushynska, Halina; Sokolov, Eugene; Piontkivska, Helen (2020): The effects of hypoxia/reoxygenation stress on activities of mitochondrial complexes I and IV and activities of protein kinases and phosphatases in marine bivalves [dataset bundled publication]. PANGAEA, https://doi.org/10.1594/PANGAEA.919121
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Abstract:
Hypoxia commonly occurs in aquatic habitats and puts strong pressure on the benthic sessile organisms unable to escape the oxygen fluctuations. Here we focused on the effects of hypoxia/reoxygenation (H/R) on activities of the mitochondrial Complexes I and IV in hypoxia-tolerant marine bivalves, namely, the blue mussel Mytilus edulis, the Arctic quahog Arctica islandica and the Pacific oyster Crassostrea gigas, exposing them for one or six days to extreme hypoxia (<0.1% O2) followed by one hour of reoxygenation. Furthermore, we examined the potential role of the posttranslational modifications (PTM) by reversible protein phosphorylation in regulation of the Complex I and IV activities and in the mitochondrial responses to H/R stress. The overall level of protein phosphorylation by PKA (serine/threonine protein kinase A) and PKC (protein kinase C) was measured to test for possible association between the H/R-induced changes in the PKA and PKC activities and the respective protein products of these kinases. Our results showed a strong species-specific modulation of the PKA and PKC activities by H/R stress. In the mussels and quahogs, phosphorylation by PKA and PKC led to an increase in activity of Complexes I and IV, whereas in oysters these enzymes were insensitive to PKA and PKC activation. Non-site-specific dephosphoryaltion strongly suppressed the activity of Complex I and IV in all three studied species.
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Sokolova, Inna M; et al. (accepted): THE ROLE OF REVERSIBLE PROTEIN PHOSPHORYLATION IN REGULATION OF THE MITOCHONDRIAL ELECTRON TRANSPORT SYSTEM DURING HYPOXIA AND REOXYGENATION STRESS IN MARINE BIVALVES. Frontiers in Marine Science
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Creative Commons Attribution 4.0 International (CC-BY-4.0)
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Datasets listed in this bundled publication
- Sokolova, I; Falfushynska, H; Sokolov, E et al. (2020): Mitochondrial complex I in hypoxia-tolerant marine bivalves exposed to extreme hypoxia. https://doi.org/10.1594/PANGAEA.919120
- Sokolova, I; Falfushynska, H; Sokolov, E et al. (2020): Mitochondrial complex IV in hypoxia-tolerant marine bivalves exposed to extreme hypoxia. https://doi.org/10.1594/PANGAEA.919123
- Sokolova, I; Falfushynska, H; Sokolov, E et al. (2020): Serine/threonine protein kinase A (PKA) and protein kinase C (PKC) in hypoxia-tolerant marine bivalves exposed to extreme hypoxia. https://doi.org/10.1594/PANGAEA.919125
- Sokolova, I; Falfushynska, H; Sokolov, E et al. (2020): Total phosphatases in hypoxia-tolerant marine bivalves exposed to extreme hypoxia. https://doi.org/10.1594/PANGAEA.919124